Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in Medaka

dc.contributor.authorPadilla, Luisa F Arias
dc.contributor.authorCastañeda-Cortés, Diana C. [UNESP]
dc.contributor.authorRosa, Ivana F. [UNESP]
dc.contributor.authorAcosta, Omar D Moreno
dc.contributor.authorHattori, Ricardo S. [UNESP]
dc.contributor.authorNóbrega, Rafael H.
dc.contributor.authorFernandino, Juan I.
dc.contributor.institutionINTECH (CONICET-UNSAM)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSao Paulo Fisheries Institute (APTA/SAA)
dc.date.accessioned2021-06-25T10:26:14Z
dc.date.available2021-06-25T10:26:14Z
dc.date.issued2021-03-01
dc.description.abstractThe production of an adequate number of gametes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key in both sexes. Cystic proliferation of germline stem cells is an especially important step prior to the beginning of meiosis; however, the molecular regulators of this proliferation remain elusive in vertebrates. Here, we report that ndrg1b is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of cystic proliferation of germ cells. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior.en
dc.description.affiliationInstituto Tecnológico de Chascomús INTECH (CONICET-UNSAM)
dc.description.affiliationReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationSalmonid Experimental Station at Campos do Jordão UPD-CJ Sao Paulo Fisheries Institute (APTA/SAA)
dc.description.affiliationUnespReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdAgencia Nacional de Promoción Científica y Tecnológica: 0366/12
dc.description.sponsorshipIdFAPESP: 14/07620-7
dc.description.sponsorshipIdFAPESP: 18/10265-5
dc.description.sponsorshipIdAgencia Nacional de Promoción Científica y Tecnológica: 2501/15
dc.description.sponsorshipIdFAPESP: D 2979/16
dc.identifierhttp://dx.doi.org/10.7554/eLife.62757
dc.identifier.citationeLife, v. 10.
dc.identifier.doi10.7554/eLife.62757
dc.identifier.issn2050-084X
dc.identifier.scopus2-s2.0-85102906236
dc.identifier.urihttp://hdl.handle.net/11449/206081
dc.language.isoeng
dc.relation.ispartofeLife
dc.sourceScopus
dc.titleCystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in Medakaen
dc.typeArtigo
unesp.author.orcid0000-0003-4689-2561[1]
unesp.author.orcid0000-0003-1754-2802[7]

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