Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis

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de Souza, Mariana C.
de Souza Mesquita, Leonardo M.
Pena, Fabíola L.
Tamborlin, Leticia [UNESP]
da Silva, Laise C.
Viganó, Juliane
Antunes, Adriane E. Costa
Luchessi, Augusto D. [UNESP]
Duarte, Marta C. Teixeira
Barbero, Gerardo F.

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The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy.



Cancer therapy, Coupled extraction techniques, Flavonoids, Theaflavin, THP-1

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Food Chemistry Advances, v. 1.