Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa

dc.contributor.authorLopes, Flavia C. M. [UNESP]
dc.contributor.authorRocha, Ana
dc.contributor.authorPirraco, Ana
dc.contributor.authorRegasini, Luis O. [UNESP]
dc.contributor.authorSilva, Dulce Helena Siqueira [UNESP]
dc.contributor.authorBolzani, Vanderlan da Silva [UNESP]
dc.contributor.authorAzevedo, Isabel
dc.contributor.authorCarlos, Iracilda Z. [UNESP]
dc.contributor.authorSoares, Raquel
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Porto
dc.date.accessioned2014-05-20T14:21:11Z
dc.date.available2014-05-20T14:21:11Z
dc.date.issued2009-05-22
dc.description.abstractBackground: Angiogenesis, a complex multistep process that comprehends proliferation, migration and anastomosis of endothelial cells (EC), has a major role in the development of pathologic conditions such as inflammatory diseases, tumor growth and metastasis. Brazilian flora, the most diverse in the world, is an interesting spot to prospect for new chemical leads, being an important source of new anticancer drugs. Plant-derived alkaloids have traditionally been of interest due to their pronounced physiological activities. We investigated the anti-angiogenic potential of the naturally occurring guanidine alkaloid pterogynidine (Pt) isolated from the Brazilian plant Alchornea glandulosa. The purpose of this study was to examine which features of the angiogenic process could be disturbed by Pt.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with 8 mu M Pt and cell viability, proliferation, apoptosis, invasion and capillary-like structures formation were addressed. Nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with Pt. Quantifications were expressed as mean +/- SD of five independent experiments and one-way analysis of variance (ANOVA) followed by the Dunnet test was used.Results: A significant decrease in proliferation and invasion capacity and an effective increase in apoptosis as assessed by bromodeoxyuridine (BrdU), double-chamber and terminal transferase dUTP nick end labeling (TUNEL) assay, respectively, have been found. Pt also led to a drastic reduction in the number of capillary-like structures formation when HUVEC were cultured on growth factor reduced-Matrigel (GFR-Matrigel) coated plates. In addition, incubation of HUVEC with Pt resulted in reduced NF kappa B activity.Conclusion: These findings emphasize the potential use of Pt against pathological situations where angiogenesis is stimulated as tumor development.en
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, Brazil
dc.description.affiliationUniv Porto, Fac Med, Dept Biochem FCT U38, Oporto, Portugal
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Organ Chem, Araraquara Inst Chem, BR-14801902 São Paulo, Brazil
dc.description.sponsorshipFCT
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipERAB, European Advisory Board
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 1008/07-2
dc.description.sponsorshipIdERAB, European Advisory Board: EA0641
dc.description.sponsorshipIdFAPESP: 03/02176-7
dc.format.extent11
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-9-15
dc.identifier.citationBmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 11, 2009.
dc.identifier.doi10.1186/1472-6882-9-15
dc.identifier.fileWOS000267598400001.pdf
dc.identifier.issn1472-6882
dc.identifier.lattes4702004904231248
dc.identifier.lattes4484083685251673
dc.identifier.orcid0000-0002-1516-7765
dc.identifier.urihttp://hdl.handle.net/11449/26333
dc.identifier.wosWOS:000267598400001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Complementary and Alternative Medicine
dc.relation.ispartofjcr2.109
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleAnti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosaen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes4702004904231248[5]
unesp.author.lattes4484083685251673
unesp.author.orcid0000-0002-1516-7765[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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