Crystallization of bothrombin, a fibrinogen-converting serine protease isolated from the venom of Bothrops jararaca

dc.contributor.authorWatanabe, L.
dc.contributor.authorVieira, D. F.
dc.contributor.authorBortoleto, R. K.
dc.contributor.authorArni, R. K.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:02:18Z
dc.date.available2014-05-20T14:02:18Z
dc.date.issued2002-06-01
dc.description.abstractBothrombin, a snake-venom serine protease, specifically cleaves fibrinogen, releasing fibrinopeptide A to form non-crosslinked soft clots, aggregates platelets in the presence of exogeneous fibrinogen and activates blood coagulation factor VIII. Bothrombin shares high sequence homology with other snake-venom proteases such as batroxobin (94% identity), but only 30 and 34% identity with human alpha-thrombin and trypsin, respectively. Single crystals of bothrombin have been obtained and X-ray diffraction data have been collected at the Laboratorio Nacional de Luz Sincrotron to a resolution of 2.8 Angstrom. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 94.81, b = 115.68, c = 155.97 Angstrom.en
dc.description.affiliationUNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, Brazil
dc.format.extent1036-1038
dc.identifierhttp://dx.doi.org/10.1107/S0907444902003645
dc.identifier.citationActa Crystallographica Section D-biological Crystallography. Copenhagen: Blackwell Munksgaard, v. 58, p. 1036-1038, 2002.
dc.identifier.doi10.1107/S0907444902003645
dc.identifier.fileWOS000176271200017.pdf
dc.identifier.issn0907-4449
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/21953
dc.identifier.wosWOS:000176271200017
dc.language.isoeng
dc.publisherBlackwell Munksgaard
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallography
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization of bothrombin, a fibrinogen-converting serine protease isolated from the venom of Bothrops jararacaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Munksgaard
unesp.author.lattes9162508978945887[4]
unesp.author.orcid0000-0003-2460-1145[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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