Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination

dc.contributor.authorSantos, I. Walter
dc.contributor.authorBinsfeld, L. C.
dc.contributor.authorWeiss, R. R.
dc.contributor.authorKozicki, L. E.
dc.contributor.authorRochadelli, R.
dc.contributor.authorResende, M. V. [UNESP]
dc.contributor.authorHossepian de Lima, V. F M [UNESP]
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPontifícia Universidade Católica do Paraná (PUCPR)
dc.date.accessioned2014-05-27T11:22:45Z
dc.date.available2014-05-27T11:22:45Z
dc.date.issued2007-12-31
dc.description.abstractThe objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm.en
dc.description.affiliationDepartamento de Medicina Veterinária UFPR, Rua Pioneiro 2153, 85950-000 Palotina, PR
dc.description.affiliationDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SP
dc.description.affiliationDepartamento de Medicina Veterinária UFPR, Curitiba, SP
dc.description.affiliationDepartamento de Medicina Veterinária PUCPR, Sao Jose dos Pinhais, PR
dc.description.affiliationUnespDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SP
dc.format.extent47-54
dc.identifierhttp://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/article/view/9909
dc.identifier.citationArchives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007.
dc.identifier.file2-s2.0-37349017260.pdf
dc.identifier.issn1517-784X
dc.identifier.scopus2-s2.0-37349017260
dc.identifier.urihttp://hdl.handle.net/11449/70229
dc.language.isoeng
dc.relation.ispartofArchives of Veterinary Science
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBitch
dc.subjectExtenders
dc.subjectOestrus
dc.subjectSemen
dc.subjectBovinae
dc.subjectCanis familiaris
dc.subjectOsiris
dc.titleCryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial inseminationen
dc.typeArtigo
dcterms.licensehttp://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/about/editorialPolicies#openAccessPolicy
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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