Development and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplification

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dc.contributor.authorSouza-Shibatta, Lenice
dc.contributor.authorFerreira, Dhiego Gomes
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.authorAlmeida, Fernanda Simoes de
dc.contributor.authorShibatta, Oscar Akio
dc.contributor.authorSofia, Silvia Helena
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:42Z
dc.date.available2014-12-03T13:08:42Z
dc.date.issued2013-06-30
dc.description.abstractThirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.en
dc.description.affiliationUniv Estadual Londrina, Dept Biol Anim & Vegetal, Ctr Ciencias Biol, Programa Posgrad Ciencias Biol, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, Ctr Ciencias Biol, Programa Posgrad Genet & Biol Mol, BR-86051990 Londrina, PR, Brazil
dc.description.affiliationUniv Paulista Julio Mesquita Filho, Inst Biociencias, Dept Biociencias, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Paulista Julio Mesquita Filho, Inst Biociencias, Dept Biociencias, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipPrograma Universal
dc.description.sponsorshipUniversidade Estadual de Londrina
dc.description.sponsorshipIBAMA/SISBIO
dc.description.sponsorshipIdPrograma Universal05/2011
dc.description.sponsorshipIdPrograma Universal22.633
dc.description.sponsorshipIdIBAMA/SISBIO12120-1
dc.format.extent581-585
dc.identifierhttp://dx.doi.org/10.1590/S1679-62252013000300011
dc.identifier.citationNeotropical Ichthyology. São Paulo: Soc Brasileira Ictiologia, v. 11, n. 3, p. 581-585, 2013.
dc.identifier.fileS1679-62252013000300011.pdf
dc.identifier.issn1679-6225
dc.identifier.scieloS1679-62252013000300011
dc.identifier.urihttp://hdl.handle.net/11449/111500
dc.identifier.wosWOS:000326973700011
dc.language.isoeng
dc.publisherSoc Brasileira Ictiologia
dc.relation.ispartofNeotropical Ichthyology
dc.relation.ispartofjcr1.216
dc.relation.ispartofsjr0,559
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectBrazilian Eastern basinen
dc.subjectCatfishen
dc.subjectEnriched genomic libraryen
dc.subjectGenetic markeren
dc.subjectSSRen
dc.titleDevelopment and characterization of microsatellite loci of microglanis cottoides (siluriformes: pseudopimelodidae) and cross-species amplificationen
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Ictiologia
unesp.author.orcid0000-0002-3443-0696[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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