Trypanosoma cruzi and Its soluble antigens induce NET release by stimulating toll-like receptors

dc.contributor.authorSousa-Rocha, Daniel [UNESP]
dc.contributor.authorThomaz-Tobias, Mariana [UNESP]
dc.contributor.authorDiniz, Larissa Figueiredo Alves [UNESP]
dc.contributor.authorSouza, Priscila Silva Sampaio [UNESP]
dc.contributor.authorPinge-Filho, Phileno
dc.contributor.authorToledo, Karina Alves [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2015-12-07T15:34:08Z
dc.date.available2015-12-07T15:34:08Z
dc.date.issued2015
dc.description.abstractNeutrophils release fibrous traps of DNA, histones, and granule proteins known as neutrophil extracellular traps (NETs), which contribute to microbicidal killing and have been implicated in autoimmunity. The role of NET formation in the host response to nonbacterial pathogens is not well-understood. In this study, we investigated the release of NETs by human neutrophils upon their interaction with Trypanosoma cruzi (Y strain) parasites. Our results showed that human neutrophils stimulated by T. cruzi generate NETs composed of DNA, histones, and elastase. The release occurred in a dose-, time-, and reactive oxygen species-dependent manner to decrease trypomastigote and increase amastigote numbers of the parasites without affecting their viability. NET release was decreased upon blocking with antibodies against Toll-like receptors 2 and 4. In addition, living parasites were not mandatory in the release of NETs induced by T. cruzi, as the same results were obtained when molecules from its soluble extract were tested. Our results increase the understanding of the stimulation of NETs by parasites, particularly T. cruzi. We suggest that contact of T. cruzi with NETs during Chagas's disease can limit infection by affecting the infectivity/pathogenicity of the parasite.en
dc.description.affiliationDepartment of Biological Sciences, Univ. Estadual Paulista-UNESP (FCL-Assis), Assis, São Paulo, Brazil
dc.description.affiliationDepartamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
dc.description.affiliationUnespDepartment of Biological Sciences, Univ. Estadual Paulista-UNESP (FCL-Assis), Assis, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/01522-8
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0139569
dc.identifier.citationPlos One, v. 10, n. 10, 2015.
dc.identifier.doi10.1371/journal.pone.0139569
dc.identifier.filePMC4591979.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes5772565774304020
dc.identifier.pmcPMC4591979
dc.identifier.pubmed26431537
dc.identifier.urihttp://hdl.handle.net/11449/131345
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourcePubMed
dc.titleTrypanosoma cruzi and Its soluble antigens induce NET release by stimulating toll-like receptorsen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes5772565774304020
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
unesp.departmentCiências Biológicas - FCLASpt

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