Comparison of conventional and extractive fermentation using aqueous two-phase system to extract fibrinolytic proteases produced by Bacillus stearothermophilus DPUA 1729

dc.contributor.authorCruz Filho, Raimundo Felipe da
dc.contributor.authordos Santos, Januário Gama
dc.contributor.authorPalheta, Rosana Antunes
dc.contributor.authorSantos-Ebinuma, Valéria Carvalho [UNESP]
dc.contributor.authorViana Marques, Daniela de Araújo
dc.contributor.authorTeixeira, Maria Francisca Simas
dc.contributor.institutionFederal University of Amazonas
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Pernambuco
dc.date.accessioned2020-12-12T01:35:59Z
dc.date.available2020-12-12T01:35:59Z
dc.date.issued2020-01-01
dc.description.abstractFibrinolytic enzymes have been considered promising for treatment and protection of healthy circulation due its ability to dissolve the fibrin in blood clots. Extractive fermentation is a not explored and efficient downstream process which segregates the desired product simultaneously in a fermentation process fast and economically. Extraction of fibrinolytic enzymes by Bacillus stearothermophilus DPUA 1729 employing conventional aqueous two-phase systems (ATPS) and extractive fermentation with ATPS was evaluated. The results of both systems were compared using a factorial design with PEG molar mass, PEG and salt concentrations as independent variables and extraction parameters as a response. In all conditions evaluated it was observed a similar partitioning of fibrinolytic enzymes through the phases, both in conventional ATPS and extractive fermentation. Salt concentration and interaction among PEG and salt concentration influenced in the partition coefficient. The fibrinolytic activity was determined by hydrolysis of fibrin in plate using the extract of one condition from extractive fermentation. The zone degradation presented a diameter of 7.03 ± 0.94 mm. In conclusion, there was no significant difference among the results obtained using conventional ATPS and extractive fermentation, however, the second one presents more advantages and can integrate production and extraction in one single step, reducing the costs.en
dc.description.affiliationCulture Collection DPUA/UFAM Federal University of Amazonas
dc.description.affiliationDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP-University Estadual Paulista
dc.description.affiliationLaboratory of Biotechnology Applied to Infectious and Parasitic Diseases Biological Science Institute University of Pernambuco
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP-University Estadual Paulista
dc.identifierhttp://dx.doi.org/10.1080/10826068.2020.1805756
dc.identifier.citationPreparative Biochemistry and Biotechnology.
dc.identifier.doi10.1080/10826068.2020.1805756
dc.identifier.issn1532-2297
dc.identifier.issn1082-6068
dc.identifier.scopus2-s2.0-85089860983
dc.identifier.urihttp://hdl.handle.net/11449/199297
dc.language.isoeng
dc.relation.ispartofPreparative Biochemistry and Biotechnology
dc.sourceScopus
dc.subjectAqueous two-phase system
dc.subjectBacillus
dc.subjectextractive fermentation
dc.subjectfibrinolytic enzymes
dc.titleComparison of conventional and extractive fermentation using aqueous two-phase system to extract fibrinolytic proteases produced by Bacillus stearothermophilus DPUA 1729en
dc.typeArtigo
unesp.author.orcid0000-0002-9703-1932[6]

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