Gene discovery in Boophilus microplus, the cattle tick - the transcriptomes of ovaries, salivary glands, and hemocytes

Nenhuma Miniatura disponível




Santos, IKFD
Valenzuela, J. G.
Ribeiro, JMC
De Castro, M.
Costa, J. N.
Costa, A. M.
Da Silva, E. R.
Neto, OBR
Rocha, C.
Daffre, S.

Título da Revista

ISSN da Revista

Título de Volume


New York Acad Sciences


The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.



Boophilus microplus, transcriptomes, salivary glands, ovary, hemocytes

Como citar

Impact of Ecological Changes on Tropical Animal Health and Disease Control. New York: New York Acad Sciences, v. 1026, p. 242-246, 2004.