Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide

dc.contributor.authorOliveira, Rodrigo Juliano
dc.contributor.authorPesarini, João Renato
dc.contributor.authorSalles, Maria José Sparça
dc.contributor.authorKanno, Tatiane Yumi Nakamura
dc.contributor.authorLourenço, Ana Carolina Dos Santos
dc.contributor.authorLeite, Véssia Da Silva
dc.contributor.authorSilva, Ariane Fernanda Da
dc.contributor.authorMatiazi, Hevenilton José
dc.contributor.authorRibeiro, Lúcia Regina [UNESP]
dc.contributor.authorMantovani, Mário Sérgio
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-09-30T18:18:30Z
dc.date.available2014-09-30T18:18:30Z
dc.date.issued2014
dc.description.abstractβ-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In the fifth and sixth weeks, the percentage ranged from 10.20-52.54% and -0.95-62.35%, respectively. Besides the chemopreventive efficiency it appears that the β-glucan, when combined with cyclophosphamide, is able to improve the reproductive performance of males verified by the significant reduction in rates of post-implantation losses and reabsorption in the mating of nulliparous females with males treated with cyclophosphamide.en
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul Núcleo de Hospital Universitário Terapia Celular e Genética Toxicológica
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul Faculdade de Medicina Dr. Hélio Mandetta
dc.description.affiliationUniversidade Federal de Mato Grosso do Sul Centro de Ciências Biológicas e da Saúde
dc.description.affiliationUniversidade Estadual de Londrina Departamento de Biologia Geral
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)
dc.format.extent111-119
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572014000100017
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 37, n. 1, p. 111-119, 2014.
dc.identifier.doi10.1590/S1415-47572014000100017
dc.identifier.fileS1415-47572014000100017.pdf
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572014000100017
dc.identifier.urihttp://hdl.handle.net/11449/109609
dc.identifier.wosWOS:000333626400017
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectmutationen
dc.subjectpost-implantation lossesen
dc.subjectchemopreventionen
dc.subjectmicronucleusen
dc.subjectnulliparous femalesen
dc.titleEffects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamideen
dc.typeArtigo

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