Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites

dc.contributor.authorLeone, Francisco Assis
dc.contributor.authorMasui, Douglas Chodi
dc.contributor.authorde Souza Bezerra, Thais Milena
dc.contributor.authorGarcon, Daniela Pereira
dc.contributor.authorValenti, Wagner Cotroni [UNESP]
dc.contributor.authorAugusto, Alessandra da Silva [UNESP]
dc.contributor.authorMcNamara, John Campbell
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:22Z
dc.date.available2014-05-20T13:12:22Z
dc.date.issued2012-04-01
dc.description.abstractWe investigated modulation by ATP, Mg2+, Na+, K+ and NH4 (+) and inhibition by ouabain of (Na+,K+)-ATPase activity in microsomal homogenates of whole zoeae I and decapodid III (formerly zoea IX) and whole-body and gill homogenates of juvenile and adult Amazon River shrimps, . (Na+,K+)-ATPase-specific activity was increased twofold in decapodid III compared to zoea I, juveniles and adults, suggesting an important role in this ontogenetic stage. The apparent affinity for ATP ( (M) = 0.09 +/- A 0.01 mmol L-1) of the decapodid III (Na+,K+)-ATPase, about twofold greater than the other stages, further highlights this relevance. Modulation of (Na+,K+)-ATPase activity by K+ also revealed a threefold greater affinity for K+ ( (0.5) = 0.91 +/- A 0.04 mmol L-1) in decapodid III than in other stages; NH4 (+) had no modulatory effect. The affinity for Na+ ( (0.5) = 13.2 +/- A 0.6 mmol L-1) of zoea I (Na+,K+)-ATPase was fourfold less than other stages. Modulation by Na+, Mg2+ and NH4 (+) obeyed cooperative kinetics, while K+ modulation exhibited Michaelis-Menten behavior. Rates of maximal Mg2+ stimulation of ouabain-insensitive ATPase activity differed in each ontogenetic stage, suggesting that Mg2+-stimulated ATPases other than (Na+,K+)-ATPase are present. Ouabain inhibition suggests that, among the various ATPase activities present in the different stages, Na+-ATPase may be involved in the ontogeny of osmoregulation in larval The NH4 (+)-stimulated, ouabain-insensitive ATPase activity seen in zoea I and decapodid III may reflect a stage-specific means of ammonia excretion since functional gills are absent in the early larval stages.en
dc.description.affiliationUniv São Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeira Preto, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, BR-11330900 Sao Vicente, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, BR-11330900 Sao Vicente, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)
dc.description.sponsorshipIdINCT ADAPTA/FAPEAM: 573976/2008-2
dc.format.extent201-215
dc.identifierhttp://dx.doi.org/10.1007/s00232-012-9431-9
dc.identifier.citationJournal of Membrane Biology. New York: Springer, v. 245, n. 4, p. 201-215, 2012.
dc.identifier.doi10.1007/s00232-012-9431-9
dc.identifier.issn0022-2631
dc.identifier.lattes0588804414769477
dc.identifier.lattes3167813441577674
dc.identifier.urihttp://hdl.handle.net/11449/341
dc.identifier.wosWOS:000303875500005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Membrane Biology
dc.relation.ispartofjcr1.638
dc.relation.ispartofsjr0,567
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subject(Na+,K+)-ATPase activityen
dc.subjectGill microsomeen
dc.subjectCation-binding siteen
dc.subjectMacrobrachium amazonicumen
dc.subjectOntogenetic stageen
dc.subjectEnvironmental salinityen
dc.titleKinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sitesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes0588804414769477
unesp.author.lattes3167813441577674[6]
unesp.author.orcid0000-0002-8526-1052[5]
unesp.author.orcid0000-0001-7002-9042[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept
unesp.departmentCiências Biológicas - IBCLPpt

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