Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites
dc.contributor.author | Leone, Francisco Assis | |
dc.contributor.author | Masui, Douglas Chodi | |
dc.contributor.author | de Souza Bezerra, Thais Milena | |
dc.contributor.author | Garcon, Daniela Pereira | |
dc.contributor.author | Valenti, Wagner Cotroni [UNESP] | |
dc.contributor.author | Augusto, Alessandra da Silva [UNESP] | |
dc.contributor.author | McNamara, John Campbell | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:12:22Z | |
dc.date.available | 2014-05-20T13:12:22Z | |
dc.date.issued | 2012-04-01 | |
dc.description.abstract | We investigated modulation by ATP, Mg2+, Na+, K+ and NH4 (+) and inhibition by ouabain of (Na+,K+)-ATPase activity in microsomal homogenates of whole zoeae I and decapodid III (formerly zoea IX) and whole-body and gill homogenates of juvenile and adult Amazon River shrimps, . (Na+,K+)-ATPase-specific activity was increased twofold in decapodid III compared to zoea I, juveniles and adults, suggesting an important role in this ontogenetic stage. The apparent affinity for ATP ( (M) = 0.09 +/- A 0.01 mmol L-1) of the decapodid III (Na+,K+)-ATPase, about twofold greater than the other stages, further highlights this relevance. Modulation of (Na+,K+)-ATPase activity by K+ also revealed a threefold greater affinity for K+ ( (0.5) = 0.91 +/- A 0.04 mmol L-1) in decapodid III than in other stages; NH4 (+) had no modulatory effect. The affinity for Na+ ( (0.5) = 13.2 +/- A 0.6 mmol L-1) of zoea I (Na+,K+)-ATPase was fourfold less than other stages. Modulation by Na+, Mg2+ and NH4 (+) obeyed cooperative kinetics, while K+ modulation exhibited Michaelis-Menten behavior. Rates of maximal Mg2+ stimulation of ouabain-insensitive ATPase activity differed in each ontogenetic stage, suggesting that Mg2+-stimulated ATPases other than (Na+,K+)-ATPase are present. Ouabain inhibition suggests that, among the various ATPase activities present in the different stages, Na+-ATPase may be involved in the ontogeny of osmoregulation in larval The NH4 (+)-stimulated, ouabain-insensitive ATPase activity seen in zoea I and decapodid III may reflect a stage-specific means of ammonia excretion since functional gills are absent in the early larval stages. | en |
dc.description.affiliation | Univ São Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeira Preto, BR-14040901 Ribeirao Preto, SP, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, BR-11330900 Sao Vicente, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, BR-11330900 Sao Vicente, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) | |
dc.description.sponsorshipId | INCT ADAPTA/FAPEAM: 573976/2008-2 | |
dc.format.extent | 201-215 | |
dc.identifier | http://dx.doi.org/10.1007/s00232-012-9431-9 | |
dc.identifier.citation | Journal of Membrane Biology. New York: Springer, v. 245, n. 4, p. 201-215, 2012. | |
dc.identifier.doi | 10.1007/s00232-012-9431-9 | |
dc.identifier.issn | 0022-2631 | |
dc.identifier.lattes | 0588804414769477 | |
dc.identifier.lattes | 3167813441577674 | |
dc.identifier.uri | http://hdl.handle.net/11449/341 | |
dc.identifier.wos | WOS:000303875500005 | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartof | Journal of Membrane Biology | |
dc.relation.ispartofjcr | 1.638 | |
dc.relation.ispartofsjr | 0,567 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | (Na+,K+)-ATPase activity | en |
dc.subject | Gill microsome | en |
dc.subject | Cation-binding site | en |
dc.subject | Macrobrachium amazonicum | en |
dc.subject | Ontogenetic stage | en |
dc.subject | Environmental salinity | en |
dc.title | Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
dcterms.rightsHolder | Springer | |
unesp.author.lattes | 0588804414769477 | |
unesp.author.lattes | 3167813441577674[6] | |
unesp.author.orcid | 0000-0002-8526-1052[5] | |
unesp.author.orcid | 0000-0001-7002-9042[6] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicente | pt |
unesp.department | Ciências Biológicas - IBCLP | pt |
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