Glufosinate Resistance Level is Proportional to Phosphinothricin Acetyltransferase Gene Expression in Glufosinate-Resistant Maize
Nenhuma Miniatura disponível
Data
2018-12-05
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
Phosphinothricin acetyltransferase (pat) gene confers resistance to glufosinate by transforming this herbicide into N-acetyl-l-glufosinate (NAG). The pat gene was inserted in six maize hybrids (Herculex, Agrisure TL, Herculex Yieldgard, Leptra, Viptera 3, Power Core) as a selectable marker, and its expression was evaluated by qPCR in comparison with the maize glufosinate-susceptible cultivar VTPRO. In addition, the levels of NAG, glufosinate degradation, ammonia accumulation, electron transport rate (ETR), visual injury, and biomass were also investigated. The VTPRO, Herculex, Agrisure, and Viptera showed lower pat gene expression, and consequently lower NAG contents and glufosinate degradation, as well as reduced ETR and biomass accumulation. In contrast, greater ammonia accumulation and higher visual injury were observed. The ranking of pat gene expression was Leptra > Power Core > Herculex Yieldgard ≫ Herculex > Agrisure TL = Viptera 3 > VTPRO. This gene expression was proportional to the glufosinate resistance level observed in each maize hybrid.
Descrição
Idioma
Inglês
Como citar
Journal of Agricultural and Food Chemistry, v. 66, n. 48, p. 12641-12650, 2018.