Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study

dc.contributor.authorBorges, Aline Chiodi [UNESP]
dc.contributor.authorde Morais Gouvêa Lima, Gabriela [UNESP]
dc.contributor.authorMayumi Castaldelli Nishime, Thalita [UNESP]
dc.contributor.authorVidal Lacerda Gontijo, Aline [UNESP]
dc.contributor.authorKostov, Konstantin Georgiev [UNESP]
dc.contributor.authorKoga-Ito, Cristiane Yumi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:21:10Z
dc.date.available2018-12-11T17:21:10Z
dc.date.issued2018-06-01
dc.description.abstractThe aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity.en
dc.description.affiliationDepartment of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemistry and Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Chemistry and Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2014/02354-7
dc.description.sponsorshipIdFAPESP: #2015/ 21989-6
dc.description.sponsorshipIdFAPESP: #2015/21989-6
dc.description.sponsorshipIdFAPESP: #2016/07196-6
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0199832
dc.identifier.citationPLoS ONE, v. 13, n. 6, 2018.
dc.identifier.doi10.1371/journal.pone.0199832
dc.identifier.file2-s2.0-85049152644.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes1946509801000450
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.scopus2-s2.0-85049152644
dc.identifier.urihttp://hdl.handle.net/11449/176515
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleAmplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo studyen
dc.typeArtigo
unesp.author.lattes1946509801000450
unesp.author.lattes6543563161403421[6]
unesp.author.orcid0000-0002-2416-2173[6]

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