Photobiomodulation of inflammatory-cytokine-related effects in a 3-D culture model with gingival fibroblasts

Cardoso, Laís Medeiros [UNESP]; Pansani, Taisa Nogueira [UNESP]; Hebling, Josimeri [UNESP]; de Souza Costa, Carlos Alberto [UNESP]; Basso, Fernanda Gonçalves

Photobiomodulation of inflammatory-cytokine-related effects in a 3-D culture model with gingival fibroblasts

Data

2020-07-01

Autores

Cardoso, Laís Medeiros

Pansani, Taisa Nogueira

Hebling, Josimeri

de Souza Costa, Carlos Alberto

Basso, Fernanda Gonçalves

Tipo

Artigo

Resumo

The aim of this study was to assess the effects of IL-6 and IL-8 cytokines on human gingival fibroblasts (HGF) cultured in a 3-D model and the possible photobiomodulation (PBM) of such effects by low-level laser therapy. In complete culture medium (DMEM), HGF from a healthy patient were seeded in a type I collagen matrix inserted into 24-well plates. After 5 days of incubation, the cytokines were added or not to serum-free DMEM, which was applied to the cell-enriched matrices. Then, PBM was performed: three consecutive irradiations using LaserTable diode device (780 nm, 0.025 W) at 0.5 J/cm2 were delivered or not to the cells. Twenty-four hours after the last irradiation, cell viability and morphology, gene expression, and synthesis of inflammatory cytokines and growth factors were assessed. The histological evaluation demonstrated that, for all groups, matrices presented homogeneous distribution of cells with elongated morphology. However, numerous cytokine-exposed cells were rounded. IL-6 and IL-8 decreased cell viability, synthesis of VEGF, and gene expression of collagen type I. PBM enhanced cell density in the matrices and stimulated VEGF expression, even after IL-6 challenge. Reduced TNF-α synthesis occurred in those cells subjected to PBM. In conclusion, PBM can penetrate collagen matrix and stimulate HGF, highlighting the relevance of this research model for further phototherapy studies and in vitro biomodulation of the healing process.