Development of HA/Ag-NPs composite coating from green process for hip applications

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Lozoya-Rodrfguez, Denisse A.
De Lima, Renata
Fraceto, Leonardo F. [UNESP]
Pérez, Antonio Ledezma
Domfnguez, Mercedes Bazaldua
Batres, Roberto Gómez
Rojas, Armando Reyes
Carmona, Víctor Orozco

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In the present study, biological hydroxyapatite (HA) was obtained from bovine bones through a thermal process. A total of 0% and 1% of silver nanoparticles (Ag-NPs) synthesized from Opuntia ficus (nopal) were added to the biological hydroxyapatite coatings using an atmospheric plasma spray (APS) on a Ti6Al4V substrate. Following this, its antimicrobial efficiency was evaluated against the following bacterial strains: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. This was conducted according to the Japanese Industrial Standard (JIS) Z2801:2000 Antimicrobial Product-Test for Antimicrobial Activity and Efficacy. Scanning electron microscopy (SEM) showed that the silver nanoparticles (Ag-NPs) were evenly distributed on the coating surface. Energy dispersive X-ray spectroscopy (EDX) shows that apatite deposition occurs on a daily basis, maintaining a Ca/P rate between 2.12 and 1.45. Biocompatibility properties were evaluated with osteoblast-like cells (MC3T3-E1) by single-cell gel electrophoresis assay and Tali image cytometry.



Antibacterial test, Atmospheric plasma spray, Biological hydroxyapatite, Coating, Cytotoxic test, Silver nanoparticles

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Molecules, v. 22, n. 8, 2017.