Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)

dc.contributor.authorEto, Silas F. [UNESP]
dc.contributor.authorFernandes, Dayanne C. [UNESP]
dc.contributor.authorMoraes, Alessandra C. [UNESP]
dc.contributor.authorPrado, Ed Johnny R. [UNESP]
dc.contributor.authorBaldassi, Amanda C. [UNESP]
dc.contributor.authorManrique, Wilson G.
dc.contributor.authorSilva, Ives C. [UNESP]
dc.contributor.authorMedeiros, Andrea S.R. [UNESP]
dc.contributor.authorBelo, Marco A.A. [UNESP]
dc.contributor.authorBalbuena, Tiago S. [UNESP]
dc.contributor.authorSamara, Samir I. [UNESP]
dc.contributor.authorPizauro, João M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionRondônia Federal University
dc.date.accessioned2018-12-11T17:18:22Z
dc.date.available2018-12-11T17:18:22Z
dc.date.issued2018-05-01
dc.description.abstractStreptococcus agalactiae (Sta), which belongs to Lancefield group B, causes sepsis, endocarditis and bacterial meningitis in human neonates and Nile tilapia. Because the pathophysiology of Sta infection is partially similar in both species, the identification of biomarkers for the diagnosis and study of this disease is of importance for human and animal health. Therefore, in the present study, we produced an immunoglobulin Y (IgY) by immunizing laying hens with Sta proteins and evaluated its ability to detect Sta in paraffinized tilapia brain and cardiac tissue by direct immunofluorescence (IMF) and indirect immunohistochemistry (IHC). The IgY produced was effective in the diagnosis of Sta infection in Nile tilapia, justifying the use of this species as a biomodel for the study of this disease.en
dc.description.affiliationDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05, Jaboticabal
dc.description.affiliationInstitute of Chemistry of Unesp
dc.description.affiliationDepartment of Preventive Veterinary Medicine of Unesp
dc.description.affiliationLaboratory of Animal Pathology Rondônia Federal University
dc.description.affiliationUnespDepartment of Technology School of Agrarian and Veterinary Sciences Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05, Jaboticabal
dc.description.affiliationUnespInstitute of Chemistry of Unesp
dc.description.affiliationUnespDepartment of Preventive Veterinary Medicine of Unesp
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 140505/2016-4
dc.description.sponsorshipIdCNPq: 429245/2016-5
dc.format.extent153-160
dc.identifierhttp://dx.doi.org/10.1016/j.fsi.2018.02.048
dc.identifier.citationFish and Shellfish Immunology, v. 76, p. 153-160.
dc.identifier.doi10.1016/j.fsi.2018.02.048
dc.identifier.file2-s2.0-85042783205.pdf
dc.identifier.issn1095-9947
dc.identifier.issn1050-4648
dc.identifier.scopus2-s2.0-85042783205
dc.identifier.urihttp://hdl.handle.net/11449/175971
dc.language.isoeng
dc.relation.ispartofFish and Shellfish Immunology
dc.relation.ispartofsjr1,126
dc.relation.ispartofsjr1,126
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntibody
dc.subjectImmunodiagnostics
dc.subjectNeuropathy
dc.subjectTeleost fish
dc.titleValidation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia (Oreochromis niloticus)en
dc.typeArtigo

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