Protic ionic liquids as constituent of aqueous two-phase system based on acetonitrile: Synthesis, phase diagrams and genipin pre-purification

Alves Camêlo, Lídia Cristina; de Souza Dias Santos, Gabriela; Lucena de Souza, Ranyere; Faria Soares, Cleide Mara; Brandão Pereira, Jorge Fernando [UNESP]; Lima, Álvaro Silva

Protic ionic liquids as constituent of aqueous two-phase system based on acetonitrile: Synthesis, phase diagrams and genipin pre-purification

Data

2020-03-01

Autores

Alves Camêlo, Lídia Cristina

de Souza Dias Santos, Gabriela

Lucena de Souza, Ranyere

Faria Soares, Cleide Mara

Brandão Pereira, Jorge Fernando

Lima, Álvaro Silva

Tipo

Artigo

Resumo

The aim of this study was to evaluate the formation of aqueous two-phase systems (ATPS) based on different protic ionic liquids (PIL), synthesized using amines, and organic or inorganic acid, and acetonitrile (ACN). The ATPSs are used to pre-purify genipin from genipap (Genipa americana L.). All PILs (purity above 98%) were able to form ATPSs with ACN, the size of the biphasic region being directly dependent on salting-out aptitude of PIL anions and relative hydrophilicity of the corresponding cations. The highest solid–liquid extraction of genipin from genipap was achieved by using aqueous ACN solution at 75% (16.79 ± 0.06 mg g−1 dry raw material) as extractant. After the extraction stage, the ATPS was used as an integrated platform to pre-purify the genipin present in the extract. Three different overall mixture point compositions were tested, and those formed by [MEA][H2Cit] (25 wt%) + ACN (45 wt%) + water (30 wt%) at 298.15 ± 1.00 K and 0.10 ± 0.01 MPa showed the highest selectivity (S = 4.202 ± 0.009) and recovery of genipin in top phase (RTG = 56.74 ± 0.04%), and the purification factor in top phase was 5.9 fold.