Detection of circulating Paracoccidioides brasiliensis antigen in urine of paracoccidioidomycosis patients before and during treatment

dc.contributor.authorSalina, Margarete Aparecida [UNESP]
dc.contributor.authorShikanai-Yasuda, Maria Aparecida
dc.contributor.authorMendes, Rinaldo Poncio [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:19:35Z
dc.date.available2014-05-27T11:19:35Z
dc.date.issued1998-06-01
dc.description.abstractFor the diagnosis and follow-up of paracoccidioidomycosis patients undergoing therapy, we evaluated two methods (immunoblotting and competition enzyme immunoassay) for the detection of circulating antigen in urine samples. A complex pattern of reactivity was observed in the immunoblot test. Bands of 70 and 43 kDa were detected more often in urine samples from patients before treatment. The immunoblot method detected gp43 and gp70 separately or concurrently in 11 (91.7%) of 12 patients, whereas the competition enzyme immunoassay detected antigenuria in 9 (75%) of 12 patients. Both tests appeared to be highly specific (100%), considering that neither fraction detectable by immunoblotting was present in urine samples from the control group. gp43 remained present in the urine samples collected during the treatment period, with a significant decrease in reactivity in samples collected during clinical recovery and increased reactivity in samples collected during relapses. Reactivity of some bands was also detected in urine specimens from patients with 'apparent cure.' The detection of Paracoccidioides brasiliensis antigens in urine appears to be a promising method for diagnosing infection, for evaluating the efficacy of treatment, and for detecting relapse.en
dc.description.affiliationDepto. de Analises Clinicas Faculdade de Cie. Farmaceuticas UNESP, Araraquara
dc.description.affiliationDepto. Doencas Infecciosas P. Faculdade de Medicina USP, São Paulo
dc.description.affiliationDepto. de Molestias Infecciosas Faculdade de Medicina UNESP, Botucatu
dc.description.affiliationLab. de Micologia Clínica Depto. de Analises Clinicas UNESP, R. Expedicionarios do Brasil, 1621, Araraquara SP, CEP 14801-902
dc.description.affiliationUnespDepto. de Analises Clinicas Faculdade de Cie. Farmaceuticas UNESP, Araraquara
dc.description.affiliationUnespDepto. de Molestias Infecciosas Faculdade de Medicina UNESP, Botucatu
dc.description.affiliationUnespLab. de Micologia Clínica Depto. de Analises Clinicas UNESP, R. Expedicionarios do Brasil, 1621, Araraquara SP, CEP 14801-902
dc.format.extent1723-1728
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC104907/
dc.identifier.citationJournal of Clinical Microbiology, v. 36, n. 6, p. 1723-1728, 1998.
dc.identifier.file2-s2.0-0031968577.pdf
dc.identifier.issn0095-1137
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.scopus2-s2.0-0031968577
dc.identifier.urihttp://hdl.handle.net/11449/65454
dc.identifier.wosWOS:000073641300044
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Microbiology
dc.relation.ispartofjcr4.054
dc.relation.ispartofsjr2,256
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectantigen detection
dc.subjectclinical article
dc.subjectdiagnostic value
dc.subjectenzyme immunoassay
dc.subjecthuman
dc.subjectimmunoblotting
dc.subjectparacoccidioides brasiliensis
dc.subjectpriority journal
dc.subjectrecurrence risk
dc.subjectreinfection
dc.subjectsouth american blastomycosis
dc.subjecturinalysis
dc.subjectAntifungal Agents
dc.subjectAntigens, Fungal
dc.subjectHumans
dc.subjectImmunoblotting
dc.subjectImmunoenzyme Techniques
dc.subjectParacoccidioides
dc.subjectParacoccidioidomycosis
dc.subjectRecurrence
dc.subjectSensitivity and Specificity
dc.subjectParacoccidioides brasiliensis
dc.titleDetection of circulating Paracoccidioides brasiliensis antigen in urine of paracoccidioidomycosis patients before and during treatmenten
dc.typeArtigo
unesp.author.lattes6840524602748457[4]
unesp.author.orcid0000-0002-9855-5594[4]
unesp.author.orcid0000-0002-8059-0826[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
2-s2.0-0031968577.pdf
Tamanho:
319.34 KB
Formato:
Adobe Portable Document Format