Microallelotyping defines novel regions of loss of heterozygosity in uterine leiomyomas

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dc.contributor.authorCanevari, R. D.
dc.contributor.authorPontes, Anaglória [UNESP]
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:35:05Z
dc.date.available2014-05-20T13:35:05Z
dc.date.issued2005-03-01
dc.description.abstractUterine leiomyomas are extremely common, benign, smooth muscle tumors that represent a significant public health problem. Although there have been few molecular studies of uterine leiomyomas, most of them have reported a very low frequency of loss of heterozygosity (LOH) in different regions of the genome. The detection of LOH has been used to identify genomic regions that harbor tumor suppressor genes and to characterize different tumor types. We have used a set of 15 microsatellite polymorphism markers to examine the frequency of allele loss in a panel of 64 human uterine leiomyomas matched to normal DNAs. The markers were chosen from regions involved in losses identified by comparative genomic hybridization in a subset of uterine leiomyomas described in a previous report. DNA from tumors and normal tissue was amplified by the polymerase chain reaction and subsequently analyzed using an ABI Prism 377 DNA automated sequencer. The frequency of LOH observed was low, except for the markers D15S87 (15q26.3), D7S493 (7p15.3), and D7S517 (7p22.2). No changes in microsatellite size were detected in our samples. These results provide useful clues for identifying putative tumor suppressor genes associated with a subset of uterine leiomyomas. (C) 2004 Wiley-Liss, Inc.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Urol, NeoGene Lab, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Obstet & Gynecol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biosci, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Urol, NeoGene Lab, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Obstet & Gynecol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biosci, Dept Genet, BR-18618000 Botucatu, SP, Brazil
dc.format.extent177-182
dc.identifierhttp://dx.doi.org/10.1002/mc.20074
dc.identifier.citationMolecular Carcinogenesis. Hoboken: Wiley-liss, v. 42, n. 3, p. 177-182, 2005.
dc.identifier.doi10.1002/mc.20074
dc.identifier.issn0899-1987
dc.identifier.lattes0514178654667684
dc.identifier.lattes2259986546265579
dc.identifier.urihttp://hdl.handle.net/11449/12043
dc.identifier.wosWOS:000227200000006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofMolecular Carcinogenesis
dc.relation.ispartofjcr3.851
dc.relation.ispartofsjr1,277
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmicroallelotypingpt
dc.subjectloss of heterozygositypt
dc.subjectuterine leiomyomaspt
dc.titleMicroallelotyping defines novel regions of loss of heterozygosity in uterine leiomyomasen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes0514178654667684
unesp.author.lattes2259986546265579
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Urologia - FMB
Artigos - Genética - IBB
Artigos - Ginecologia e Obstetrícia - FMB