Apoptosis in the early developing periodontium of rat molars

dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.authorFreymüller, Edna
dc.contributor.authorKatchburian, Eduardo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:19:53Z
dc.date.available2014-05-27T11:19:53Z
dc.date.issued2000-02-01
dc.description.abstractDevelopment of the periodontium involves a series of complex steps that result in the formation of root dentine, cementum, bone and fibres of the ligament. These precisely controlled and timed events require the participation of the enamel organ derived epithelial cells of Hertwig's (HRS) and ectomesenchymal cells of the dental follicle. These events involve rapid turnover of the tissues and cells, including disappearance of epithelial cells of HRS. Thus, it seemed likely to us that programmed cell death (apoptosis) may play a role in the development of the periodontium. Fragments of first molars, obtained from 14- and 29-day-old rats, were fixed in glutaraldehyde-formaldehyde and processed for light and electron microscopy. For the TUNEL method for detection of apoptosis, specimens were fixed in 4% formaldehyde and embedded in paraffin. Results confirmed that epithelial cells of HRS maintain a close relationship with the forming dentine root, and that they may become trapped in the dentino-cemental junction. Some of the epithelial cells exhibited ultrastructural features which are consistent with the interpretation that they were undergoing programmed cell death, i.e. apoptosis. Periodontal fibroblast-like cells showed typical images of apoptosis and engulfed apoptotic bodies. TUNEL positive structures were present in all corresponding regions. It seems therefore that apoptosis of epithelial cells of HRS and fibroblast-like cells of the periodontal ligament constitutes an integral part of the developmental process of the tissues of the periodontium. (C) 2000 Wiley-Liss, Inc.en
dc.description.affiliationDepartment of Morphology São Paulo State University, São Paulo
dc.description.affiliationElectron Microscopy Centre Fed. University of São Paulo, 04023-900, São Paulo
dc.description.affiliationDepartment of Morphology Fed. University of São Paulo, 04023-900, Säo Paulo
dc.description.affiliationDepartment of Morphology School of Medicine Fed. University of São Paulo, Rua Botucatu 740, 04023-900 São Paulo, S.P.
dc.description.affiliationUnespDepartment of Morphology São Paulo State University, São Paulo
dc.format.extent136-144
dc.identifierhttp://dx.doi.org/10.1002/(SICI)1097-0185(20000201)258:2<136
dc.identifier.citationAnatomical Record, v. 258, n. 2, p. 136-144, 2000.
dc.identifier.doi10.1002/(SICI)1097-0185(20000201)258:2<136
dc.identifier.file2-s2.0-0342632812.pdf
dc.identifier.issn0003-276X
dc.identifier.lattes3278495911207882
dc.identifier.orcid0000-0001-5756-5828
dc.identifier.scopus2-s2.0-0342632812
dc.identifier.urihttp://hdl.handle.net/11449/66093
dc.language.isoeng
dc.relation.ispartofAnatomical Record
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectApoptosis
dc.subjectCementum
dc.subjectHertwig's root sheath
dc.subjectPeriodontal ligament
dc.subjectPeriodontium
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectcementum
dc.subjectenamel
dc.subjectepithelium cell
dc.subjectmolar tooth
dc.subjectnonhuman
dc.subjectperiodontium
dc.subjectpriority journal
dc.subjectrat
dc.subjecttooth development
dc.subjecttooth root
dc.subjectAging
dc.subjectAnimals
dc.subjectDental Enamel
dc.subjectIn Situ Nick-End Labeling
dc.subjectMicroscopy, Electron
dc.subjectMolar
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectAnimalia
dc.titleApoptosis in the early developing periodontium of rat molarsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes3278495911207882[1]
unesp.author.orcid0000-0001-5756-5828[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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