Detecção e caracterização molecular de Cryptosporidium spp. em canários mantidos em cativeiro (Serinus canaria) por diferentes métodos de diagnóstico

dc.contributor.authorCamargo, Vinícius da Silva [UNESP]
dc.contributor.authorSantana, Bruna Nicoleti [UNESP]
dc.contributor.authorFerrari, Elis Domingos [UNESP]
dc.contributor.authorNakamura, Alex Akira
dc.contributor.authorNagata, Walter Bertequini [UNESP]
dc.contributor.authorNardi, Ana Rita Moraes
dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdades Adamantinenses Integradas
dc.contributor.institutionFundação Municipal de Ensino Superior
dc.date.accessioned2018-12-11T17:36:36Z
dc.date.available2018-12-11T17:36:36Z
dc.date.issued2018-01-01
dc.description.abstractThis study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather’s solution. Cryptosporidium spp. diagnosis was performed using three diagnostic methods: malachite green negative staining, nested PCR targeting the 18S rRNA gene, followed by sequencing the amplified fragments, and duplex real-time PCR targeting the 18S rRNA specific to detect Cryptosporidium galli and Cryptosporidium avian genotype III. The overall positivity for Cryptosporidium spp. (total samples positive in at least one protocol) from the microscopic analysis, nested PCR and duplex real-time PCR protocol results was 13.3% (66/498). The positivity rates were 2.0% (10/498) and 4.6% (23/498) for Cryptosporidium spp. by microscopy and nested PCR, respectively. Sequencing of 20 samples amplified by nested PCR identified C. galli (3.0%; 15/498), Cryptosporidium avian genotype I (0.8%; 4/498) and Cryptosporidium avium (0.2%; 1/498). Duplex real-time PCR revealed a positivity of 7.8% (39/498) for C. galli and 2.4% (12/498) for avian genotype III. Malachite green negative staining differed significantly from nested PCR in detecting Cryptosporidium spp. Duplex real-time PCR was more sensitive than nested PCR/sequencing for detecting gastric Cryptosporidium in canaries.en
dc.description.affiliationFaculdade de Medicina Veterinária Universidade Estadual Paulista – UNESP
dc.description.affiliationCurso de Medicina Veterinária Faculdades Adamantinenses Integradas
dc.description.affiliationFundação Municipal de Ensino Superior
dc.description.affiliationUnespFaculdade de Medicina Veterinária Universidade Estadual Paulista – UNESP
dc.format.extent61-66
dc.identifierhttp://dx.doi.org/10.1590/S1984-296120180010
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 27, n. 1, p. 61-66, 2018.
dc.identifier.doi10.1590/S1984-296120180010
dc.identifier.fileS1984-29612018000100060.pdf
dc.identifier.issn0103-846X
dc.identifier.lattes0903513897615274
dc.identifier.scieloS1984-29612018000100060
dc.identifier.scopus2-s2.0-85045050113
dc.identifier.urihttp://hdl.handle.net/11449/179748
dc.language.isoeng
dc.relation.isnodouble153972*
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBirds
dc.subjectCryptosporidiosis
dc.subjectDiagnosis
dc.subjectEpidemiology
dc.titleDetecção e caracterização molecular de Cryptosporidium spp. em canários mantidos em cativeiro (Serinus canaria) por diferentes métodos de diagnósticopt
dc.title.alternativeDetection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methodsen
dc.typeArtigo
unesp.author.lattes0903513897615274

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