ORIGINAL ARTICLE In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese Sabrina Neves Casarotti1,2 & Bruno Moreira Carneiro3 & Svetoslav Dimitrov Todorov4 & Luis Augusto Nero4 & Paula Rahal5 & Ana Lúcia Barretto Penna1 Received: 10 August 2016 /Accepted: 7 February 2017 /Published online: 28 February 2017 # Springer-Verlag Berlin Heidelberg and the University of Milan 2017 Abstract The aim of this study was to evaluate the safety and probiotic potential characteristics of ten Lactobacillus spp. strains (Lactobacillus fermentum SJRP30, Lactobacillus casei SJRP37, SJRP66, SJRP141, SJRP145, SJRP146, and SJRP169, and Lactobacillus delbrueckii subsp. bulgaricus SJRP50, SJRP76, and SJRP149) that had previously been isolated from water buffalo mozzarella cheese. The safety of the strains was analyzed based on mucin degradation, hemo- lytic activity, resistance to antibiotics and the presence of genes encoding virulence factors. The in vitro tests concerning probiotic potential included survival under simulated gastro- intestinal (GI) tract conditions, intestinal epithelial cell adhe- sion, the presence of genes encoding adhesion, aggregation and colonization factors, antimicrobial activity, and the pro- duction of the β-galactosidase enzyme. Although all strains presented resistance to several antibiotics, the resistance was limited to antibiotics to which the strains had intrinsic resis- tance. Furthermore, the strains presented a limited spread of genes encoding virulence factors and resistance to antibiotics, and none of the strains presented hemolytic or mucin degra- dation activity. The L. delbrueckii subsp. bulgaricus strains showed the lowest survival rate after exposure to simulated GI tract conditions, whereas all of the L. casei and L. fermentum strains showed good survivability. None of the tested lactobacilli strains presented bile salt hydrolase (BSH) activity, and only L. casei SJRP145 did not produce the β-galactosidase enzyme. The strains showed varied levels of adhesion to Caco-2 cells. None of the cell-free supernatants inhibited the growth of path- ogenic target microorganisms. Overall, L. fermentum SJRP30 and L. casei SJRP145 and SJRP146 were revealed to be safe and to possess similar or superior probiotic characteristics com- pared to the reference strain L. rhamnosus GG (ATCC 53103). Keywords Lactic acid bacteria . Dairy . Safety . Antibiotic resistance . Beneficial properties . Gastrointestinal tract survival Introduction Lactobacillus spp. belong to the group of lactic acid bacteria (LAB) and have a long history of use in the production of dairy products due to their ability to convert lactose into lactic acid (Tulumoğlu et al. 2014). In addition to their use as tech- nological agents in the food industry, some Lactobacillus spe- cies can confer health benefits to the host when they are ad- ministered adequately as probiotics. Probiotics are currently defined Bas live microorganisms that, when administered in adequate amounts, confer health benefit on the host^ (Hill et al. 2014). Although probiotics have been extensively * Sabrina Neves Casarotti sabrinacasarotti@yahoo.com.br * Ana Lúcia Barretto Penna analucia@ibilce.unesp.br 1 Departamento de Engenharia e Tecnologia de Alimentos, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), R. Cristovão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo, Brazil 2 Departamento de Alimentos e Nutrição, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil 3 Instituto de Ciências Exatas e Naturais, Universidade Federal de Mato Grosso (UFMT), 78735-901 Rondonópolis, MT, Brazil 4 Departamento de Veterinária, Universidade Federal de Viçosa (UFV), 36570-000 Viçosa, MG, Brazil 5 Departamento deBiologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP, Brazil Ann Microbiol (2017) 67:289–301 DOI 10.1007/s13213-017-1258-2 http://crossmark.crossref.org/dialog/?doi=10.1007/s13213-017-1258-2&domain=pdf studied and commercialized, and are the subject of national and international regulations, there is no agreement concerning the amount of probiotic bacteria necessary to pro- duce their beneficial effects. Generally, probiotic food prod- ucts must contain 106 CFU/mL or CFU/g (Shah 2000). Nevertheless, some authors state that beneficial effects can be achieved even when bacteria lose their viability (Adams 2010). Some of the health effects attributed to probiotic consump- tion include the regulation of gastrointestinal (GI) functions, relief of lactose intolerance, prevention of different types of diarrhea besides urogenital infections, reduction in cholesterol levels, reduction in atopic and food allergies, and modulation of the immune system. Furthermore, in vitro studies have shown that probiotic bacteria reduce the number of pathogens and their metabolic activities in the human intestine and com- pete with these microorganisms for attachment sites to intes- tinal epithelial cells and nutrients (Guarner and Malagelada 2003; Mishra et al. 2015). Although a large number of probiotic strains are available for commercial use worldwide, the isolation and characteriza- tion of new strains from different species is desirable; thus, many studies in this field have been published in recent years (Jeronymo-Ceneviva et al. 2014; Peres et al. 2014; de Paula et al. 2015; Oh and Jung 2015). Probiotics targeted for human consumption are usually isolated from humans or animals because strains from these origins can better adapt to the con- ditions encountered in the human/animal GI tract, which en- ables more successful gut colonization (Argyri et al. 2013). However, certain food-associated Lactobacillus strains have probiotic characteristics even though they do not belong to the gut microbiota (Solieri et al. 2014; Tulumoğlu et al. 2014). According to the FAO/WHO (2002), a bacterial strain should fulfill a number of requirements to be considered pro- biotic; these requirements must be verified by in vitro and in vivo tests. In vitro tests are useful for the selection of strains that have greater probiotic potential; these tests increase knowledge regarding the strain as well as the mechanisms underlying the beneficial effects. Although LAB, particularly Lactobacillus, are generally recognized as safe (GRAS), ad- ditional tests should be performed to check the safety of these strains because some cases recently associated systemic infec- tion with the consumption of probiotics (Liong 2008; Sharma and Devi 2014). Thus, evaluating their safety, assessing their resistance to antibiotics, investigating the presence of viru- lence genes, and determining hemolytic activity are important (Jeronymo-Ceneviva et al. 2014; Vijayakumar et al. 2015). Given these points, the aim of this study was to characterize the safety features and probiotic potential attributes of autoch- thonous Lactobacillus spp. isolated from water buffalo moz- zarella cheese using in vitro tests. Candidates that met the established criteria may be used in the production of fermented products to promote their probiotic characteristics. Materials and methods Bacterial strains Ten Lactobacillus strains previously isolated and identified through 16S rRNA gene sequencing by our group (Silva et al. 2015; Silva 2015) as Lactobacillus fermentum (SJRP30), Lactobacillus casei (SJRP37, SJRP66, SJRP141, SJRP145, SJRP146, and SJRP169), and Lactobacillus delbrueckii subsp. bulgaricus (SJRP50, SJRP76 and SJRP149) were screened for their safety and probiotic poten- tial. Lactobacillus rhamnosusGG (ATCC 53103) was used as a probiotic reference strain. The strains were maintained at −80 °C in MRS broth (Difco, Becton Dickinson, Sparks, MD) supplemented with 25% (v/v) glycerol (Vetec, Duque de Caxias, RJ, Brazil). Each culture was sub-cultured at least twice in MRS broth before use in the assays. Assessment of safety characteristics Hemolytic activity Fresh lactobacilli broth cultures (8.0–9.0 log CFU/mL) were streaked in triplicate on Columbia agar plates containing 5% (w/v) sheep blood (NewProv, Pinhais, PR, Brazil). After 48 h of incubation at 37 °C, the plates were examined for hemolytic reactions. The Lactobacillus rhamnosus GG (ATCC 53103) and Staphylococcus aureus ATCC 6538 strains were used as the negative and positive controls, respectively (Pieniz et al. 2014). The assay was repeated on three independent occasions in triplicate. Mucin degradation Mucin degradation was determined according to Zhou et al. (2001). Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 and Lactobacillus rhamnosus GG (ATCC 53103) were used as the positive and negative controls, respectively. The assay was repeated on three inde- pendent occasions in triplicate. Presence of genes encoding virulence factors, antibiotic resistance and biogenic amines The Lactobacillus strains were tested for the presence of vir- ulence, antibiotic resistance and amino acid decarboxylase genes (Table 1). DNA was extracted using the QIAgen DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), followed by DNA concentration estimation using the NanoDrop2000 spectrophotometer (Thermo Scientific, Waltham, MA). PCRs were performed according to the refer- ences listed in Table 1, and the amplified products were sep- arated by electrophoresis in 0.8 to 2.0% (w/v) agarose gels in 290 Ann Microbiol (2017) 67:289–301 T ab le 1 Pr es en ce of ge ne s im pl ic at ed in vi ru le nc e fa ct or s, an tib io tic re si st an ce an d bi og en ic am in e pr od uc tio n in La ct ob ac ill us sp p. st ra in s G en e E nc od ed fa ct or L. fe rm en tu m L. ca se i L. de lb ru ec ki is ub sp .b ul ga ri cu s R ef er en ce S JR P3 0 S JR P 37 S JR P 66 S JR P 14 1 S JR P1 45 SJ R P 14 6 S JR P1 69 SJ R P 50 S JR P7 6 S JR P 14 9 V ir ul en ce ge lE G el at in as e –a – + – – – – – + + V an ke rc kh ov en et al .( 20 04 ) hy l H ya lu ro ni da se – – – – – – – + + – V an ke rc kh ov en et al .( 20 04 ) as a1 A gg re ga tio n su bs ta nc e – – – – – – – + – – V an ke rc kh ov en et al .( 20 04 ) es p E nt er oc oc ca ls ur fa ce pr ot ei n – + + + – – + – – – V an ke rc kh ov en et al .( 20 04 ) cy lA C yt ol ys in – – – – – – + – – – V an ke rc kh ov en et al .( 20 04 ) ef aA E nd oc ar di tis an tig en – – – – – – – – – – M ar tín -P la te ro et al .( 20 09 ) ac e A dh es io n of co lla ge n – – – – – – – – – – M ar tín -P la te ro et al .( 20 09 ) fs rA G el at in as e – + + + + – + – – – L op es et al .( 20 06 ) fs rB G el at in as e – + + + – – + – – – L op es et al .( 20 06 ) fs rC G el at in as e – – – – – – – + + – L op es et al .( 20 06 ) sp rE Se ri ne pr ot ea se – – – – + – – – – + L op es et al .( 20 06 ) cc f Se x ph er om on es ,c he m ot ac tic fo r hu m an le uk oc yt es ;f ac ili ta te co nj ug at io n – + – + + + – + – – E at on an d G as so n (2 00 1) co b Se x ph er om on es ,c he m ot ac tic fo r hu m an le uk oc yt es ;f ac ili ta te co nj ug at io n – – – – – – – + – + E at on an d G as so n (2 00 1) cp d Se x ph er om on es ,c he m ot ac tic fo r hu m an le uk oc yt es ;f ac ili ta te co nj ug at io n – + – + – + + + – – E at on an d G as so n (2 00 1) B io ge ni c am in e hd c1 H is tid in e de ca rb ox yl as e – + – – – – + – – + de L as R iv as et al .( 20 05 ) hd c2 H is tid in e de ca rb ox yl as e – – – – – – – – – – de L as R iv as et al .( 20 05 ) td c Ty ro si ne de ca rb ox yl as e – – – – – – – + + – de L as R iv as et al .( 20 05 ) od c O rn ith in e de ca rb ox yl as e – – – – – – – – – – de L as R iv as et al .( 20 05 ) A nt ib io tic re si ta nc e va n A V an co m yc in re si st an ce – + + + – – + – – – M ar tín -P la te ro et al .( 20 09 ) va n B V an co m yc in re si st an ce – + + + – + + – – – M ar tín -P la te ro et al .( 20 09 ) va nC 1 V an co m yc in re si st an ce – – – + – – – + + + D ut ka -M al en et al .( 19 95 ) va nC -2 ,v an C -3 V an co m yc in re si st an ce + – – – – – + – – – D ut ka -M al en et al .( 19 95 ) va nC 1 V an co m yc in re si st an ce + – – – – – + – – – M ie le et al .( 19 95 ) te t (M ) Te tr ac yc lin e re si st an ce – – – – – – – – + – A ar es tr up et al .( 20 00 b) te t(L ) Te tr ac yc lin e re si st an ce – – – + – – – + + – A ar es tr up et al .( 20 00 b) Ann Microbiol (2017) 67:289–301 291 T ab le 1 (c on tin ue d) G en e E nc od ed fa ct or L. fe rm en tu m L. ca se i L. de lb ru ec ki is ub sp .b ul ga ri cu s R ef er en ce SJ R P3 0 SJ R P3 7 SJ R P6 6 SJ R P 14 1 SJ R P1 45 S JR P1 46 SJ R P1 69 SJ R P5 0 SJ R P7 6 SJ R P 14 9 te t(K ) Te tr ac yc lin e re si st an ce – + – – – – – + – + A ar es tr up et al .( 20 00 b) te t(O ) Te tr ac yc lin e re si st an ce – + + + + + + – – – A ar es tr up et al .( 20 00 b) te t(S ) Te tr ac yc lin e – – – – – – – – – – A ar es tr up et al .( 20 00 a) bc r( B ) B ac itr ac in re si st an ce – – + – + + + – – – M an so n et al .( 20 04 ) bc r( D ) B ac itr ac in re si st an ce – – – – – – – – – – M an so n et al .( 20 04 ) bc r( R ) B ac itr ac in re si st an ce – – – – – + – – – – M an so n et al .( 20 04 ) er m (A ) E ry th ro m yc in re si st an ce – – – – – – – – – – Su tc lif fe et al .( 19 96 ) er m (B ) E ry th ro m yc in re si st an ce – – – – – – – – – – Su tc lif fe et al .( 19 96 ) er m (C ) E ry th ro m yc in re si st an ce – + – + + + + – + – Su tc lif fe et al .( 19 96 ) er m (B ) E ry th ro m yc in re si st an ce – – – – – – – – – – G ev er s et al .( 20 03 ) an t(4 ′)- Ia A m in og ly co si de re si st an ce + + + + + – + – – – Fo rt in a et al .( 20 08 ) ap h( 3′ )- II I- a A m in og ly co si de re si st an ce – + – – – – + + + + Fo rt in a et al .( 20 08 ) ap h( 2″ )- Ib A m in og ly co si de re si st an ce + + – + + + – – – – Fo rt in a et al .( 20 08 ) ap h( 2″ )- Ic A m in og ly co si de re si st an ce – + + + + – + – – – Fo rt in a et al .( 20 08 ) ap h( 2″ )- Id A m in og ly co si de re si st an ce – – – + + – – + + + Fo rt in a et al .( 20 08 ) aa c( 6′ )- Ie -a p- h( 2″ )- Ia A m in og ly co si de re si st an ce + + + – + – – + – + Fo rt in a et al .( 20 08 ) aa c( 6′ )- Ii A m in og ly co si de re si st an ce + + + + + – + + + – C os ta et al .( 19 93 ) ca tA (P IP 50 1) C hl or am ph en ic ol re si st an ce – + + – – – – – – – A ar es tr up et al .( 20 00 a) in t-T n Te tr ac yc lin e re si st an ce – – + – – – – – – + Fo rt in a et al .( 20 08 ) in t T ra ns po so n in te gr as e ge ne + + + + – + – + + + G ev er s et al .( 20 03 ) a + In di ca te s th e pr es en ce an d – ab se nc e of ge ne s 292 Ann Microbiol (2017) 67:289–301 0.5× TAE buffer. The gels were stained in 0.5× TAE buffer containing 0.5 μg/mL of ethidium bromide (Sigma-Aldrich, St. Louis, MO). Antibiotic susceptibility The disc diffusion assay was applied to determine the antibi- otic susceptibility of the strains. Diluted culture (100 μL; 6.0 log CFU/mL) was spread onto MRS agar media (Difco), and antibiotic discs (Oxoid, Basingstoke, UK) containing (per disc) ampicillin (10 μg), vancomycin (30 μg), gentamicin (10 μg), kanamycin (30 μg), streptomycin (300 μg), tetracy- cline (30 μg), chloramphenicol (30 μg), erythromycin (15 μg), and clindamycin (2 μg) were placed manually on the surface of the inoculated plates using sterile forceps. These antibiotics were chosen according to the list proposed by the European Food Safety Authority (EFSA 2012). The plates were incubated at 37 °C under anaerobic conditions, and the diameters of the inhibition zones were evaluated 24 h after incubation. The susceptibility of the isolates was scored as resistant, moderately susceptible, or susceptible ac- cording to the cut-off values proposed by Charteris et al. (1998). The assay was repeated on three independent occa- sions in triplicate. Assessment of probiotic potential characteristics Tolerance to simulated GI tract conditions The tolerance to simulated GI tract conditions test was per- formed by successively exposing the strains to gastric and enteric simulated juices as described by Botta et al. (2014). The lactobacilli strains were grown for 18 h at 37 °C in MRS broth, and 1 mL of each culture (8.0–9.0 CFU/mL) was dis- tributed into four sterile flasks (two for the gastric phase and two for the enteric phase). The solutions simulating the gastric and enteric juices were prepared according to the method of Bautista-Gallego et al. (2013). The pH values used in the gastric and enteric phases were 2.5 and 8.0, respectively. All enzyme solutions were prepared and filter-sterilized using a 0.22-μmmembrane filter (Merck Millipore, Cork, Ireland) on the day of analysis. The cells were counted at the beginning (T0) and the end of the gastric phase (T120) and after the enteric phase (T360). The cell count was performed by serial dilution and plating in MRS agar (Difco). The plates were incubated at 37 °C for 48 h under anaerobic conditions (Anaerobac, Probac, São Paulo, Brazil). The commercial probiotic L. rhamnosus GG (ATCC 53103) was used as a reference strain. The assay was repeated on three independent occasions in duplicate. Bile salt hydrolase activity Fresh bacterial cultures of the studied lactobacilli (8.0–9.0 log CFU/mL) were screened for bile salt hydrolase (BSH) activity as previously described by de Paula et al. (2014) using MRS plates supplemented with taurodeoxycholic acid sodium salt (TDCA) or taurocholic acid sodium salt hydrate (TC); MRS plates without TDCA and TC were used as negative controls, whereas L. mesenteroides SJRP 55 was used as a positive control. The plates were incubated anaerobically at 37 °C for 48 h. The presence of precipitated bile acid around the spots was considered a positive result (Rodríguez et al. 2012). The assay was repeated on three independent occasions in triplicate. Adhesion to Caco-2 cells The Caco-2 cell line BCRJ 0059 (Rio de Janeiro Cell Bank, Rio de Janeiro, Brazil) was cultured (passages 29–31) in Dulbecco’s modified Eagle’s minimum (DMEM, Sigma- Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Cultilab, Campinas, Brazil), a mixture of penicillin (100 UI/mL) and streptomycin (100 μg/mL) (Sigma- Aldrich), and 1% non-essential amino acid solution (Sigma- Aldrich) at 37 °C in a 5% CO2 atmosphere. The adhesion assay was performed as described by Argyri et al. (2013). All bacterial cultures were grown for 18 h in MRS at 37 °C before the assays, harvested by centrifugation (7000 g, 7 min, 5 ° C), washed twice with phosphate-buffered saline (PBS) and re-suspended in DMEM without any serum or antibiotics. The commercial probiotic L. rhamnosus GG (ATCC 53103) was used as a reference strain. Subsequently, 1 mL containing approximately 8.0–9.0 log CFU bacterial cells was added to each well, and each strain was evaluated for adherence in duplicate wells in each experiment. After incubation for 2 h at 37 °C, the cells were washed three times with sterile PBS to remove non-adherent bacteria, and then detached from each well by the addition of 1 mLTriton X-100 (0.5% v/v) (Sigma-Aldrich). Following incubation for 5 min at 37 °C, the cell lysates were serially diluted and plated on MRS agar. Bacterial adhesion (%) was calculated by the ratio of adhered bacteria to the total number of added bacteria. The experiment was performed on three independent occasions. Presence of genes encoding adhesion, aggregation and colonization factors The investigated Lactobacillus strains were tested for the pres- ence of adhesion, aggregation and colonization genes (Table 2) as described in the section BPresence of genes encoding virulence factors, antibiotic resistance and biogenic amines^. Ann Microbiol (2017) 67:289–301 293 Antimicrobial activity All lactobacilli strains were tested for antimicrobial activity against Escherichia coli ATCC 25922, E. coli ATCC 8739, Listeria innocua ATCC 33090, Listeria monocytogenes ATCC 15313, Klebsiella pneumoniae subsp. pneumoniae ATCC 10031, Staphylococcus aureus subsp. aureus ATCC 25923, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 and Shigella sonnei ATCC 25931 according to the method described by de Paula et al. (2014). The antibiotic ciprofloxacin (5 μg) was used as a positive control, whereas MRS broth adjusted to pH 6.5 and filtered was used as a negative control. The assay was repeated on three independent occasions in triplicate. β-Galactosidase activity The β-galactosidase activity of the Lactobacillus spp. strains was assessed by employing sterile filter paper discs impreg- nated with o-nitrophenyl-β-D-galactopyranose (ONPG Discs, Fluka, Buchs, Switzerland) according to the manufacturer’s instructions. The test was performed in three independent ex- periments in duplicate. S. enterica subsp. enterica serovar Typhimurium ATCC 14028 and E. coli ATCC 25922 were used as the negative and positive controls, respectively. Statistical analysis The statistical analysis was performed using the Statistica 7.0 software (StatSoft, Inc., 2004, Tulsa, OK). One-way ANOVA followed by Tukey’s test was applied to detect significant differences (P ≤ 0.05) in the data regarding tolerance to simu- lated GI tract conditions and in vitro adhesion to Caco-2 cells. Results Hemolytic activity None of the examined strains revealed β-hemolytic (i.e., red blood cell lysis) activity when grown in Columbia sheep blood agar. Most of the strains (L. fermentum SJRP30 and L. casei strains SJRP37, SJRP66, SJRP145, SJRP146, and SJRP169) wereγ-hemolytic (i.e., no hemolysis), whereas four strains showed partial hemolysis (L. delbrueckii subsp. bulgaricus SJRP50, SJRP76, and SJRP149 and L. casei SJRP141). Staphylococcus aureus ATCC 6538 (positive con- trol) showed hemolytic activity. Mucin degradation Neither the Lactobacillus spp. nor the reference strain (nega- tive control) showed mucinolytic activity in either type ofT ab le 2 Pr es en ce of ge ne s im pl ic at ed in ad he si on ,a gg re ga tio n an d co lo ni za tio n in La ct ob ac ill us sp p. st ra in s G en e E nc od ed fa ct or L. fe rm en tu m L. ca se i L. de lb ru ec ki is ub sp .b ul ga ri cu s R ef er en ce S JR P3 0 S JR P 37 S JR P6 6 S JR P 14 1 S JR P1 45 SJ R P 14 6 S JR P 16 9 S JR P5 0 S JR P 76 SJ R P 14 9 M ub A dh es io n pr ot ei ns –a – – – – – – + – + R am ia h et al .( 20 07 ) m ap A A dh es io n pr ot ei ns – – – – + – – + – + R am ia h et al .( 20 07 ) E F -T u E lo ng at io n fa ct or – + + + + + – + + + R am ia h et al .( 20 07 ) E F 26 62 -c bp C ho lin e bi nd in g pr ot ei n – – – – – – – – – + F or tin a et al .( 20 08 ) E F 12 49 -fb p Fi br in og en bi nd in g pr ot ei n – – – – + + – – + F or tin a et al .( 20 08 ) E F 23 80 -m az M em br an e- as so ci at ed zi nc m et al lo pr ot ea se – – + + – + – + – – F or tin a et al .( 20 08 ) pr gB Su rf ac e pr ot ei n – – – – – + – – – – F or tin a et al .( 20 08 ) a + In di ca te s th e pr es en ce an d – ab se nc e of ge ne s 294 Ann Microbiol (2017) 67:289–301 tested medium (with or without glucose). Conversely, S. enterica subsp. enterica serovar Typhimurium ATCC 14028 (positive control) was able to degrade mucin in vitro in a medium in which mucin was the only energy source. Presence of genes encoding virulence factors, antibiotic resistance and biogenic amines None of the tested isolates presented a positive result for the efaA, ace, hdc2, odc, tet(S), erm(A), and erm(B) genes, which were related to endocarditis antigen, collagen adhesion, tyro- sine decarboxylase, ornithine decarboxylase, tetracycline re- sistance and erythromycin resistance, respectively (Table 1). L. casei SJRP169 had the highest frequency of genes encoding virulence factors, antibiotic resistance and biogenic amines (42.55%). Conversely, the L. fermentum SJRP30 strain showed the lowest frequency (14.89%) of positive results. Antibiotic susceptibility All of the strains were sensitive to ampicillin, tetracycline, chloramphenicol, erythromycin, and clindamycin, which are frequently used to treat bacterial infections (Table 3). All of the L. delbrueckii subsp. bulgaricus strains were sensitive to vancomycin and gentamicin, whereas the other strains were resistant. Most strains were susceptible to streptomycin, with the exception of L. fermentum SJRP30, which was classified as moderately susceptible. All of the tested strains were clas- sified as resistant to kanamycin. Tolerance to simulated GI tract conditions There was a significant decrease (P ≤ 0.05) in the popula- tions of all strains evaluated after consecutive exposure to the gastric and small intestine conditions (Fig. 1). The L. delbrueckii subsp. bulgaricus strains showed the lowest population at the end of the in vitro assay, with a cell count reduction of 3.38 log CFU/mL on average. In contrast, L. casei and L. fermentum showed good viability during the simulated GI digestion, with reductions of 0.85–2.48 log units (Fig. 1). Two groups of tolerance were outlined after exposure to simulated gastric juice at pH 2.5; L. casei SJRP37, SJRP66, SJRP141, SJRP145, SJRP146 and SJRP169, L. delbrueckii subsp. bulgaricus SJRP76 and L. rhamnosus GG maintained the same populations, whereas L. fermentum SJRP30 and L. delbrueckii subsp. bulgaricus SJRP50 and SJRP149 showed a significant (P ≤ 0.05) reduction in their populations. Tolerance to the enteric condition was variable among the strains. The L. fermentum SJRP30 and L. casei SJRP146 strains suf- fered a reduction of less than 1 log unit after exposure to simulated enteric juice. The other L. casei strains and L. delbrueckii subsp. bulgaricus SJRP50 suffered a reduc- tion between 1 and 2 log units, whereas L. delbrueckii subsp. bulgaricus SJRP76 and SJRP149 revealed a reduc- tion of 3.07 and 3.59 log CFU/mL, respectively. BSH activity All L. casei and L. fermentum strains were able to grow in MRS agar plates containing 0.5% (w/v) TDCA sodium salts, whereas the growth of L. delbrueckii subsp. Table 3 Antibiotic susceptibilitya of Lactobacillus spp. strains. AMPAmpicillin, VA vancomycin, CN gentamicin, K kanamycin, S streptomycin, TE tetracycline, C chloramphenicol, E erythromycin, DA clindamycin Species Strains AMP (10 μg) VA (30 μg) CN (10 μg) K (30 μg) S (300 μg) TE (30 μg) C (30 μg) E (15 μg) DA (2 μg) L. fermentum SJRP30 29 S 0 R 9 R 0 R 14 MS 26 S 27 S 27 S 27 S L. casei SJRP37 29 S 0 R 9 R 0 R 19 S 30 S 29 S 30 S 27 S SJRP66 32 S 0 R 13 S 0 R 23 S 35 S 31 S 37 S 34 S SJRP141 33 S 0 R 12 R 0 R 26 S 36 S 29 S 37 S 33 S SJRP145 28 S 0 R 10 R 0 R 20 S 31 S 27 S 31 S 27 S SJRP146 33 S 0 R 13 S 0 R 22 S 38 S 32 S 37 S 32 S SJRP169 35 S 0 R 14 S 0 R 25 S 36 S 34 S 36 S 33 S L. delbrueckii subsp. bulgaricus SJRP50 28 S 21 S 10 R 11 R 24 S 31 S 29 S 32 S 30 S SJRP76 27 S 21 S 10 R 7 R 23 S 31 S 30 S 32 S 29 S SJRP149 38 S 23 S 10 R 0 R 25 S 31 S 30 S 33 S 31 S L. rhamnosus GG ATCC 53103 30 S 0 R 10 R 0 R 22 S 33 S 31 S 32 S 28 S a Inhibition zones were measured in millimeters, and the susceptibility of the isolates was scored as resistant (R), moderately susceptible (MS) and susceptible (S) according to the cut-off values proposed by Charteris et al. (1998) Ann Microbiol (2017) 67:289–301 295 bulgaricus was completely inhibited. Conversely, all strains grew in MRS agar plates with 0.5% (w/v) TC. Nevertheless, none of the tested lactobacilli strains showed BSH activity. Adhesion to Caco-2 cells All of the tested strains could adhere to Caco-2 cells, but they did so to different degrees. The adhesion rates ranged from 2.59% to 18.58%. L. fermentum SJRP30, L. casei strains SJRP37, SJRP145, and SJRP146 and L. delbrueckii subsp. bulgaricus SJRP76 showed similar adherence to the reference strain after 2 h of incubation (P ≤ 0.05). L. casei SJRP141 was the most adhesive strain and presented adherent bacteria counts higher than the positive control L. rhamnosus GG. L. casei SJRP66 and L. delbrueckii subsp. bulgaricus SJRP149 had the lowest adhesion capacities. The results indi- cate that adhesion properties are strain-specific because the strains do not show similar adhesion values even though they are from the same species or genus (Fig. 2). Presence of genes encoding adhesion, aggregation and colonization factors L. delbrueckii subsp. bulgaricus SJRP149 showed positive results for all tested genes encoding adhesion, aggregation and colonization factors with the exception of the prgB gene. In contrast, strain L. fermentum SJRP30 did not harbor any of these genes. The other studied strains possessed at least one of the genes (Table 2). Antimicrobial activity The growth of pathogenic target microorganisms was not inhibited by any of the cell-free supernatants (CFS) (adjusted to pH 6.5) obtained from the tested LAB strains (data not shown). β-galactosidase activity Strains L. casei SJRP146 and L. delbrueckii subsp. bulgaricus SJRP50 and SJRP76 displayed an intense yellow color in their Fig. 1 Survival of Lactobacillus spp. strains before ( ) and during exposure to in vitro simulated gastric conditions for 120 min ( , pH 2.5) and enteric conditions for 360 min ( , pH 8). Different capital letters denote significant differences (P ≤ 0.05) among strains during the same sampling period of the in vitro assay. Different lower case letters denote significant differences (P ≤0.05) among sampling periods for the same strain in the in vitro assay. The results are expressed as the mean ± SD. n = 3 296 Ann Microbiol (2017) 67:289–301 tests. The other strains, except for L. casei SJRP145, also showed positive results; however, these strains produced a less intense yellow color. The reference strain L. rhamnosus GG and L. casei SJRP145 did not present β-galactosidase activity. Discussion In this study, we performed an in vitro analysis to determine the safety and probiotic potential characteristics of ten Lactobacillus strains. The assays were chosen based on inter- national guidelines for evaluation of probiotic potential (FAO/ WHO 2002). Although a large number of studies has been published in this field in the past, the identification of new strains with probiotic potential is always desirable, mainly because each strain shows different methods of action and several benefits to health. Nevertheless, before being used as a probiotic, the safety of the strains needs to be assessed to ensure that they will not represent a risk to consumer health. Recently, some cases relating infections to probiotic consump- tion have been reported (Kochan et al. 2011; Zbinden et al. 2015). Therefore, determining whether a strain is safe is of great concern among researchers. A lack of hemolytic activity is considered a safety require- ment when selecting a probiotic strain (FAO/WHO 2002) be- cause such bacteria are not virulent, and the lack of hemolysin ensures that opportunistic virulence will not appear among strains (Peres et al. 2014). Previous reports also revealed that different LAB species did not show hemolysis (Argyri et al. 2013; Bautista-Gallego et al. 2013; Ryu and Chang 2013; Ilavenil et al. 2015). The production of enzymes capable of degrading mucin was proposed as a determinant factor of virulence for some enteropathogens. Therefore, this property is not considered a desirable feature for probiotic strains be- cause it contributes to changes in the intestinal mucosal barrier in addition to favoring mucosal invasion by pathogens and other toxic agents (Monteagudo-Mera et al. 2012; Peres et al. 2014). Our results suggest that the evaluated probiotic candidates may not be able to invade the intestinal mucosa. These findings were in agreement with previous studies that investigated mucin degradation by several LAB species (Fernández et al. 2005; Abe et al. 2010; Rodríguez et al. 2012). The main concern regarding probiotic safety is the resis- tance to antibiotics because these strains may transfer anti- biotic resistance genes to pathogenic bacteria in the intesti- nal habitat, which can represent a serious risk for the treat- ment of infected patients. Antibiotic resistance is consid- ered a negative characteristic for probiotics (Lee et al. 2014). The strains were found to be resistant to vancomycin, gentamicin, streptomycin, and kanamycin; however, this resistance pattern is considered an intrinsic feature of LAB because it is chromosomally encoded and, thus, the corre- sponding genes will not be transferred to pathogens (Tulini et al. 2013; Botta et al. 2014; Sharma et al. 2015). Taking these reports into consideration, the resistance to vancomy- cin, kanamycin, streptomycin, and gentamicin found in the strains and in the reference strain L. rhamnosus GG can be considered acceptable. During the course of testing the strains for the presence of virulence genes, at least one of the genes responsible for gelatinase production (gelE, fsrA, fsrB and fsrC) was detected in all strains except L. fermentum SJPR30. The gelE gene is responsible for the production of gelatinase, which is an en- zyme that hydrolyzes gelatin and collagen. Moreover, gelE expression is thought to be regulated in a cell density- dependent manner by the products of fsrA, fsrB and fsrC. However, the presence of gelE does not seem to be sufficient for gelatinase activity, and a complete fsr operon may be man- datory for gelE expression (Lopes et al. 2006). In our study, none of the strains contained the complete fsr operon. Only L. casei SJRP169 presented the cylA gene; howev- er, four isolates (L. casei SJRP141 and L. delbrueckii subsp. bulgaricus SJRP50, SJRP76 and SJRP149) showed partial hemolysis in the phenotypic test, whereas SJRP169 did not. Other lytic genes most likely cause this hemolytic reaction in the phenotypic tests when the cylA gene is not expressed (Perin et al. 2014). Four L. casei strains contained the esp gene, which may be a result of horizontal transference by the Enterococcus genus. Conversely, the adhesion proper- ties conferred by the esp gene can be a significant charac- teristic for potential probiotic bacteria (de Paula et al. 2014). Sex pheromone genes (ccf, cob and cpd) were present in some of the evaluated strains. These genes are also consid- ered virulence factors because they might induce an inflam- matory response. Moreover, these genes have shown Fig. 2 Adhesion capacity of Lactobacillus spp. strains to Caco-2 cells. The adhesion capacity is calculated using the ratio of the number of bacterial cells that remained attached to the total number of bacterial cells added initially to each well. Different lower case letters denote significant differences (P ≤ 0.05) among the adhesion capacities of the strains. The results are expressed as the mean ± SD. n = 3 Ann Microbiol (2017) 67:289–301 297 in vitro chemotactic activity for human and rat polymorpho- nuclear leukocytes, and elicited superoxide production and the secretion of lysosomal enzymes (Eaton and Gasson 2001). Genes encoding antibiotic resistance were also tested. The antibiotic resistance genes vanA and vanB were fre- quently present among the tested strains. Almost all of the L. casei strains showed positive results for both genes, ex- cept L. casei SJRP145 and L. casei SJRP146 in which the vanA gene was absent. The vanC genes were harbored by L. fermentum SJRP30, L. casei SJRP141 and SJRP169 and all of the L. delbrueckii subsp. bulgaricus strains. The vanA phenotype is characterized by a higher resistance level to vancomycin than the vanB phenotype and cross-resistance to teicoplanin. The gene cluster for both vanA and vanB resistance is usually located on a plasmid that is transfer- able, and thus represents a major concern for safety due to the spread of antibiotic resistance via horizontal gene trans- fer (Klein et al. 2000; Perin et al. 2014). Conversely, vanC is located in the bacterial chromosome (Martín-Platero et al. 2009). Therefore, despite the observation of resistance dur- ing the disc diffusion test, the resistance towards vancomy- cin in L. fermentum SJRP30 and L. casei SJRP145 recorded in our study was considered intrinsic, chromosomally encoded, and not inducible or transferable (Tynkkynen et al. 1998). The lack of genes encoding vancomycin resis- tance has been reported (Casado Muñoz et al. 2014). However, other studies have demonstrated the presence of vanA and vanB genes in a variety of LAB (Jeronymo- Ceneviva et al. 2014; Perin et al. 2014). In this study, all strains contained at least one gene encoding aminoglycoside resistance, which could be associ- ated with the intrinsic resistance towards this antibiotic class among Lactobacillus spp. The most common tetracycline re- sistance gene among Lactobacillus spp. strains was tet(O), which was detected in all of the L. casei strains. However, tet(S) was not recorded in any of the strains. Tetracycline resistance genes have been found in other Lactobacillus spp. strains isolated from fermented dry sausages, cheese and yo- gurt (Zonenschain et al. 2009; Zhou et al. 2012). The int gene was detected in eight strains, indicating that these strains might harbor the transposon responsible for tet gene dissem- ination. Although the int gene has not been found in Lactobacillus strains to date, it has been identified in Enterococcus and Lactococcus strains with food origins (Bulajić et al. 2015; Morandi et al. 2015; Jaimee and Halami 2016). Regarding erythromycin resistance, only the erm(C) genewas found among the Lactobacillus sp. strains. This gene was previously detected in a variety of Lactobacillus species (Kastner et al. 2006; Klare et al. 2007; Egervärn et al. 2009). The hdc1 and tdc genes, which are related to biogenic amine production, were present in five strains. The hdc1, hdc2, tdc and odc genes express enzymes that degrade histamine, tyramine and ornithine into biogenic amines, re- spectively. Low levels of biogenic amines in food are not considered a serious risk to the consumer; however, they can be toxic when present in high concentrations (50–100 mg) (Jeronymo-Ceneviva et al. 2014). The presence of these genes has been reported for other LAB (Coton et al. 2010; Jeronymo-Ceneviva et al. 2014). Concerning the probiotic potential characteristics, the eval- uated strains showed good resistance to simulated gastric juice. In response to stress caused by acid, LAB use various mechanisms to overcome the damage, including maintaining the intracellular pH and cell membrane functionality and in- ducing stress-response proteins (Wu et al. 2014). These mech- anisms vary both within species and according to exogenous conditions, including the growth media and incubation condi- tions (Madureira et al. 2011). The negative effect of bile on the viability of most lactobacilli was more accentuated than the effect of low pH. The bile concentration in the human body usually ranges from 0.3% to 0.5% (García-Ruiz et al. 2014); therefore, probiotic bacteria must to be able to withstand these bile concentrations. Bile is a toxic component that damages the membrane by modifying its integrity and permeability. Additionally, bile disturbs the stability of macromolecules, including RNA, DNA and proteins, and may cause oxidative stress (Begley et al. 2006). Generally, L. casei strains showed higher viability after successive exposures to gastric and intestinal conditions than L. delbrueckii subsp. bulgaricus. The survival of the selected strains, with the exception of L. delbrueckii subsp. bulgaricus SJRP149, was greater than the survival observed for the com- mercial strain used as a reference; similar results were obtain- ed by Argyri et al. (2013). The good tolerance of Lactobacillus strains to gastric juice and bile is in accordance with the results reported in a previous study (Jensen et al. 2012). Nevertheless, no recovery in cell viability was ob- served for any of the strains in the subsequent treatment with enteric juice, which was in contrast to reports by other authors (Corsetti et al. 2008; Bautista-Gallego et al. 2013). This dis- crepancy most likely occurred because the strains did not have the ability to metabolize conjugated bile salts. The ability to hydrolyze bile salts is usually included as one of the criteria for the selection of strains with probiotic potential (Rodríguez et al. 2012). Nonetheless, BSH activity is rare among bacteria that have been isolated from environments with an absence of bile, such as the strains used in the present study. This finding is in agreement with other studies (Bautista-Gallego et al. 2013; Solieri et al. 2014). Adhesion to intestinal epithelial cells is commonly includ- ed as an in vitro test to select probiotic strains. Although in vitro adhesion assays are useful for providing information on the differences among the strains being assessed, the results obtained from adhesion tests are different from the reality in vivo. The human GI tract has defense systems, resident 298 Ann Microbiol (2017) 67:289–301 flora, and bowel movements that may change the strain adhe- sion ability (Jensen et al. 2012). Moreover, different values for lactobacillus adhesion to Caco-2 cells have been reported, ranging from less than 1% to more than 70% (Jensen et al. 2012; Nikolic et al. 2012; Ramos et al. 2013; Tulumoğlu et al. 2014), possibly due to variation in the conditions used during the assay, such as the type of cell line, incubation time and number of probiotic cells added. The presence of genes encoding adhesion, aggregation and colonization factors is desirable in LAB and can also indicate that the bacteria are able to adhere to the mucus layer (Jeronymo-Ceneviva et al. 2014). However, we did not find a correlation between the in vitro adhesion assay and the pres- ence of adhesion genes because L. delbrueckii subsp. bulgaricus SJRP149 had the lowest ability to adhere to the Caco-2 cell model, despite presenting genes encoding adhe- sion, aggregation and colonization factors (except the prgB gene). Although in vitro tests are considered useful indicators of strain adhesion, they do not always reflect the ability of the bacteria to adhere to the mucus covering intestinal cells (Ramiah et al. 2007). Antimicrobial activity was not detected in any of the neutralized CFSs, leading to the conclusion that no anti- microbial peptides or bacteriocin-like compounds were produced by these strains. This result is consistent with findings for other LAB strains, including Leuconostoc mesenteroides, Ln. pseudomesenteroides, L. plantarum, L. pentosus, L. paraplantarum, and L. paracasei subsp. paracasei (Argyri et al. 2013; Briggiler Marcó et al. 2014). Additionally, Ren et al. (2014) reported that most of the neutralized supernatants (pH 6.5) from lactobacilli and washed lactobacilli cells resuspended in fresh MRS broth lost their inhibitory activities against E. coli, B. cereus, and S. aureus when compared with fresh over- night lactobacilli strain cultures. The production of β-galactosidase was investigated be- cause the ability to produce this enzyme is an advantageous feature for probiotic strains. β-Galactosidase hydrolyzes lac- tose and is important for both consumers of dairy products who have lactose intolerance and for the production of dairy products. Taking all of the results into account, L. fermentum SJRP30 and L. casei SJRP145 and SJRP146 are considered safe for future application as probiotics in co-culture with starter strains according to the tests suggested by FAO/WHO (ATCC 53103). Additionally, the selected strains possessed similar or superior probiotic potential characteristics com- pared to the reference strain L. rhamnosusGG. The promising results found for these strains suggest that additional in vitro or in vivo tests should be performed to verify the possible beneficial effects toward human health, including cholesterol-reducing ability, immunomodulatory effects and lowering the risk of GI diseases. 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Abstract Introduction Materials and methods Bacterial strains Assessment of safety characteristics Hemolytic activity Mucin degradation Presence of genes encoding virulence factors, antibiotic resistance and biogenic amines Antibiotic susceptibility Assessment of probiotic potential characteristics Tolerance to simulated GI tract conditions Bile salt hydrolase activity Adhesion to Caco-2 cells Presence of genes encoding adhesion, aggregation and colonization factors Antimicrobial activity β-Galactosidase activity Statistical analysis Results Hemolytic activity Mucin degradation Presence of genes encoding virulence factors, antibiotic resistance and biogenic amines Antibiotic susceptibility Tolerance to simulated GI tract conditions BSH activity Adhesion to Caco-2 cells Presence of genes encoding adhesion, aggregation and colonization factors Antimicrobial activity β-galactosidase activity Discussion References