Revista Brasileira de Herbicidas, v.12, n.1, p.78-86, jan./abr. 2013 (ISSN 2236-1065) DOI: http://dx.doi.org/10.7824/rbh.v12i1.175 www.rbherbicidas.com.br Association effects of glyphosate and phosphite in maize plants1 Efeitos da associação de glyphosate e fosfito em plantas de milho Giovanna Larissa Gimenes Cotrick Gomes2; Edivaldo Domingues Velini3; Caio Antonio Carbonari4; Maria Lucia Bueno Trindade5 Abstract - The objective of this study was to evaluate the effects of glyphosate herbicide association with potassium phosphite in maize plants. The experiment was accomplished in green house conditions, with seeding of Pioneer 30F53H simple hybrid in vases. Experimental design used was entirely randomized with six treatments and four replications. Treatments application was made with plants in V7 developmental stage. Evaluations of electron transport rate (ETR) in photo system II were conducted during periods of 1, 2, 5, 32, 56, 80, 104, 128, 152, 176, 200 and 360 hours after application (HAA), as well as visual determinations of intoxication in the same plants at 2, 4, 6, 10 and 15 days after application (DAA). At the end of experiment (15 DAA) it was determined plants height with a graduated ruler from soil level as far as the insertion of last leaf fully expanded, and leaves and stalks dry mass. Glyphosate association with potassium phosphite Fosway® (3.0 L ha-1, with 30% of P2O5 and 20% of K2O) with 72 g a.e. ha-1 dose increased the levels of plant intoxication. Maize plants submitted to 72 g a.e. ha-1 dose alone showed higher growth than control. ETR of treated plants showed an increase in the first hours after application, and subsequent reduction in plants exposed to higher doses of glyphosate associated or not with potassium phosphite. Keywords: electron transport rate, phytotoxicity, Zea mays L. Resumo - O objetivo deste estudo foi avaliar os efeitos da associação do herbicida glyphosate com fosfito de potássio em plantas de milho. O experimento foi conduzido em casa-de-vegetação, com a semeadura do híbrido simples Pioneer 30F53H em vasos. O delineamento experimental utilizado foi inteiramente casualizado com seis tratamentos e quarto repetições. A aplicação dos tratamentos foi realizada com as plantas em estágio V7. Foram realizadas avaliações da taxa de transporte de elétrons (TTE) no fotossistema II nos períodos de 1, 2, 5, 32, 56, 80, 104, 128, 152, 176, 200 e 360 horas após a aplicação (HAA) e também se efetuou determinações visuais de intoxicação das mesmas plantas aos 2, 4, 6, 10 e 15 dias após a aplicação (DAA). No final do experimento (15 DAA) determinou-se a altura das plantas com régua graduada rente ao solo até a inserção da última folha totalmente expandida, e a massa seca de folhas e colmos. A associação do fosfito de potássio Fosway® (3,0 L ha-1, com 30% de P2O5 e 20% de K2O) com o glyphosate na dose de 72 g e.a. ha-1 aumentou os níveis de intoxicação das plantas. As plantas de milho submetidas à dose de 72 g e.a. ha-1 isolada, apresentaram maior crescimento do que as da 1 Recieved for publication in 12/07/2012 and accepted in 21/10/2012. 2 Doctor Science Academic from Programa de Pós-Graduação em Agronomia (Agricultura) da Faculdade de Ciências Agronômicas (FCA/UNESP), Botucatu/SP. E-mail: . (Corresponding Author). 3 Adjunt Professor, FCA/UNESP Botucatu/SP. 4 Assistent Professor, FCA/UNESP Botucatu/SP. 5 Engineer Agronomist. Drª., Bioativa Pesquisa e Compostos Bioativos Ltda Botucatu/SP. Gomes et al. 79 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 testemunha. O TTE das plantas tratadas apresentou incremento nas primeiras horas após a aplicação, e posterior redução nas plantas submetidas a 720 g e.a. ha-1 de glyphosate associada ou não ao fosfito de potássio. Palavras - chave: taxa de transporte de elétrons, fitotoxicidade, Zea mays L. Introduction Brazilian agribusiness, relative to cultivated area with maize crop, stands out in second place, with soybean crop reaching the first one. Brazil produced 54 million tons in 2009/2010 agricultural year, being the fourth largest world producer. Countries with the biggest productions are United States and China, with 324 and 162 million tons, respectively (AGRIANUAL, 2010). Among registered herbicides for weeds control in maize crop, glyphosate applied in maize crop pre-planting and in weeds post- emergence points out. This product belongs to the chemical group of replaced glycine, classified as non-selective and systemic action, with action mechanism that inhibits 5- enolpiruvil chiquimato 3-phosphate synthase (EPSPs) enzyme with consequent accumulation of shikimic acid (Franz et al., 1997). EPSPs enzyme inhibition by glyphosate action, affects metabolic pathway of shikimic acid that produces the three aromatic amino acid, phenylalanine, tyrosine and tryptophan. Besides it, this pathway is responsible for the synthesis of phenolic compounds that may represent up to 35% of vegetal biomass (Boudet et al., 1985). Shikimic acid accumulation in plants exposed to glyphosate represents an expressive carbon drain in Calvin cycle, by the deviation of erythrose-4-phosphate that would be employed in ribulose-1,5-bisphosphate regeneration. This is an important effect of the inhibition caused by glyphosate, drastically reducing the photosynthetic production (Geiger et al., 1986; Geiger et al., 1987; Serviates et al., 1987; Shieh et al., 1991). Due to the fact that glyphosate is considered a non-selective herbicide, it may cause injury in crops without resistance. Thus some authors have shown a possible effect of phosphite in intoxication reducing of some crops when applied with glyphosate herbicide (Castro, 2007; Ibrahim et al., 2010a; Ibrahim et al., 2010b). However, Lucas et al. (1979), observed severe intoxication in maize plants when treated with phosphite and injuries were very similar to those that glyphosate causes on the plants. This fact may be explained by the study of Mitchell & Adams (2004), which asserted that glyphosate has a phosphite group in its structural formula that could cause injury in plants. This study aimed to evaluate the association effects of glyphosate herbicides with potassium phosphite in maize plants. Material and Methods Experiments were conducted in greenhouse conditions, at Núcleo de Pesquisas Avançadas em Matologia (NUPAM), belonging to Faculdade de Ciências Agronômicas campus de Botucatu/SP da Universidade Estadual “Julio de Mesquita Filho” (FCA/UNESP), during August and December, 2010. Maize genotype used in the experiments was Pioneer 30F53H simple hybrid, cultivated in vases containing 5 L of Bioplant substrate (organic material of vegetable origin and distended vermiculite) with pH 5.7 (± 0.5). Maize sowing was accomplished on August 25, 2010, seeding six seeds per vase. At 14 days after sowing, plants were submitted to roughing, maintaining only two plants in each experimental unit. Experimental design used was entirely randomized with six treatments and four replications. Studied treatments were: glyphosate (72 g a.e. ha-1); glyphosate (720 g Gomes et al. 80 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 a.e. ha-1); glyphosate (72 g a.e. ha-1) + potassium phosphite (3 L ha-1 c.p.); glyphosate (720 g a.e. ha-1) + potassium phosphite (3 L ha- 1 c.p.); potassium phosphite (3 L ha-1 c.p.) and a check without application. Commercial products used for spray solution preparation were Roundup Original (360 g a.e. L-1) and potassium phosphite Fosway®, with 30% of P2O5 and 20% of K2O. When plants reached seven fully expanded leaves (V7 developmental stage), at 57 days after sowing, occurred treatments application, by using a stationary spray, consisting of metallic structure (spray bar), with 2.0 m wide, that travels through an useful area of 6.0 m2 in the direction of its length. The bar was equipped with four spray nozzles XR 110.02 VS, spaced at 0.5 m from each other, and willing to 0.5 m tall in relation to maize plants. Operating pressure used by the equipment was 2.0 kgf cm-2, with a speed of 3.6 km h-1 and spray solution consumption of 200 L ha-1. Evaluations of electron transport rate (ETR) in photo system II during periods of 1, 2, 5, 32, 56, 80, 104, 128, 152, 176, 200 and 360 hours after application (HAA) were conducted. Equipment used in this evaluation was a portable fluorometer (Multi-Mode Chlorophyll Fluorometer OS5p – Opti Sciences®), with readings accomplished in six points of two leaves per plant, one mature and one young, using Yield Protocol. Visual assessments of intoxication were also carried out in the same plants at 2, 4, 6, 10 and 15 DAA, by using a notes percentage scale of notes, where "0" corresponds to any injury and "100" means plant death. At the end of the experiment it was determined plant height with a graduated ruler from soil level as far as the insertion of last leaf fully expanded. Leaves and stalks of each plant were collected and packed in paper bags, and dried in forced air chamber at 60°C for seven days. Subsequently, dry material was weighed on accuracy electronic scales (0.001 g) for determination of dry matter from aerial part. For the results of electron transport rate (ETR) in distinct periods, it was established the confidence intervals for t test at 10% probability. In order to determine confidence interval, it was used the following equation: CI = (t x stdev)/nr root, where: CI = confidence interval; t = t value tabled, at 10% probability level; stdev = standard deviation; root nr = square root of repetitions number. Results of dry mass, plant height, and phytotoxicity of maize plants were submitted to variance analysis by "F" test at 5% probability and when it was significant, means were compared by t test at 10% probability. Results and Discussion According to the results, it was observed that at 72 g ha-1 of glyphosate dose plants were little affected with small chlorotic spots in young leaves. The association of this dose with phosphite, disagreeing with results presented by Ibrahim et al. (2010a,b) in pupunha palm cabbage plants, caused greater intoxication in maize plants than the application of isolated glyphosate. At 15 DAA, plants submitted to glyphosate application (72 g ha-1) showed intoxication around 1.3%, however, when it was associated with phosphite, intoxication was 16.3%. Plants showed high levels of intoxication when subjected to 720 g ha-1 dose of isolated glyphosate and in association with phosphite. At 15 DAA, plants intoxication submitted to glyphosate application was 14.3% higher than those where herbicide was associated with phosphite. Maize plants did not show intoxication symptoms during evaluated periods, after potassium phosphite application isolated (Table 1). Gomes et al. 81 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 Table 1. Percentage of maize plants intoxication submitted to glyphosate and phosphite application associated or isolated. Treatments Intoxication (%) 2 DAA1 4 DAA 6 DAA 10 DAA 15 DAA Check/Control 0,0 a 0,0 a 0,0 a 0,0 a 0,0 a glyphosate (72 g ha-1) 3,0 b 3,0 a 0,5 a 1,3 a 1,3 a glyphosate (72 g ha-1)+phosphite (3 L ha-1) 5,0 b 9,3 b 7,5 b 21,3 b 16,3 b glyphosate (720 g ha-1) 14,3 d 17,5 c 23,8 c 53,8 c 84,3 d glyphosate (720 g ha-1)+phosphite (3 L ha-1) 11,3 c 20,8 c 28,5 d 65,0 d 70,0 c Phosphite (3 L ha-1) 0,0 a 0,0 a 0,0 a 0,0 a 0,0 a F treatment 36,587** 39,940** 79,602** 100,757** 87,133** C.V. (%) 35,19 33,90 28,72 24,65 28,69 D. M. S. 2,404 3,498 3,537 7,116 10,071 ** Significant by F test at 1% probability level; * significant by F test at 5% probability level; 1Days after treatments application. Means followed by the same letter in column do not differ by t test at 10% probability level. It was observed greater accumulation of stalk dry mass, and plant height from those submitted to 72 g ha-1 of isolated glyphosate, when compared to plants without application (Table 2). For the association of this same dose with phosphite, it was verified significant reduction of plant height, around 54% in relation to the check, evidencing high levels of phytotoxicity. Several papers demonstrate stimulation of plant growth in relation to low doses glyphosate herbicide (Schanbenberger et al., 1999; Wagner et al., 2003; Cedergreen et al., 2007; Velini et al., 2008). Partial blockage of EPSPs, through the application of glyphosate reduced doses, implicated in growth stimulation of various plant species including eucalyptus, pines, maize and soybean (no transgenic and susceptible to glyphosate). The stimulation of plant growth by low doses of a toxic compound is called "hormesis" (Velini et al., 2010). Table 2. Stalks and leaves dry mass (g) and height (cm) of maize plants submitted to glyphosate and phosphite application with and without association. Treatments Leaves dry mass (g) Stalks dry mass (g) Height (cm) Check/Control 20,0 a 28,8 b 104,3 b glyphosate (72 g ha-1) 21,3 a 44,5 a 136,0 a glyphosate (72 g ha-1) + phosphite (3 L ha-1) 20,3 a 31,8 b 47,8 c glyphosate (720 g ha-1) 10,8 c 12,5 c 41,5 c glyphosate (720 g ha-1) + phosphite (3 L ha-1) 12,8 bc 14,3 c 42,5 c phosphite (3 L ha-1) 17,5 ab 34,8 ab 114,5 b F treatment 3,210* 6,191** 52,916** C.V. (%) 28,48 35,74 14,25 D. M. S. 5,965 12,162 14,172 ** Significant by F test at 1% probability level; * significant by F test at 5% probability level. Means followed by the same letter in column do not differ by t test at 10% probability level. Although the stimulation of plant growth reported in these works occur due to the application of glyphosate low doses of (1.8 to 36 g ha-1), growth stimulation observed for maize in this work was for 72 g ha-1 dose. According to Velini et al. (2010), low doses of glyphosate are not recommended as growth stimulants for crops, because the dose that causes the stimulating effect may range considerably depending on many factors, Gomes et al. 82 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 including weather, genotype, development stage and glyphosate formulation. However, Magalhães et al. (2001) did not observe increase in variables related to growth and development of maize plants (height, leaf area and dry matter) submitted to sub doses of glyphosate application. Plants subjected to the application of glyphosate higher dose (720 g ha-1) associated with phosphite showed inferior losses of stalk and leaves dry mass, although not statistically significant, than to those who were submitted to the same herbicide dose not associated with phosphite. This behavior confirms the minor intoxication observed in plants subjected to this dose associated with phosphite and, in this way, it promoted a protection of these plants against herbicide phytotoxicity effect. Phosphite isolated application did not result in significant differences for stalks and leaves dry mass and plants height, when compared with the check (Table 2). In Figures 1 and 2 are presented the electron transport rate (ETR) in photo system II of young and mature leaves, respectively, after glyphosate and phosphite application. Values are expressed as a percentage in relation to control. Young leaves were the first to show intoxication symptoms after glyphosate application (720 g ha-1) isolated and in association with phosphite, occurring reduction of 20% for electron transport rate at 2 DAA. Maximum reduction of ETR occurred 200 hours after application (HAA), approximately 8 DAA, with reduction around 70% in relation to the check, remaining until the last evaluated period. In study conducted by Zobiole et al. (2010), it was observed reduction of pants photosynthetic rate reduction of soybean plants glyphosate-resistant after the application of this herbicide, with more pronounced decrease with doses increasing used in the experiment (450, 675, 900, 1350 and 1800 g ha-1). For glyphosate application (72 g ha-1) associated with phosphite, it was observed a reduction of 20% in ETR, with subsequent recovery until 350 HAA (15 DAA). However when applying the same dose of glyphosate reported previously, not associated with phosphite, authors did not observe ETR reductions in relation to the check or control during the evaluated period. Figure 1. Electron transport rate (ETR) of young leaves from maize plants submitted to glyphosate and phosphite application associated and isolated. In relation to mature leaves (Figure 2), it was observed a reduction in ETR, around 20%, five days after glyphosate application (720 g ha-1), isolated and in association with 0 20 40 60 80 100 120 140 0 50 100 150 200 250 300 350 ET R (% in re la tio n to th e ch ec k) Hours after application (HAA) Glyphosate 72 g ha-1 Glyphosate 720 g ha-1 Glyphosate 72 g ha-1 + Phosphite Glyphosate 720 g ha-1 + Phosphite Phosphite Gomes et al. 83 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 phosphite. For the application of isolated glyphosate, it was observed greater reduction (70%) at final period of the experiment, when compared with combined application with phosphite, with maximum reduction of ETR, in approximately 45%. All other treatments did not differ from the check/ control, and this way, there was not change in electrons transportation rate in maize plants. It was observed that ETR reduction, both in young and mature leaves (Figures 1 and 2), was accompanied by increased of plants intoxication during the evaluated period (Table 1). Shieh et al. (1991), reported for Beta vulgaris, that the accumulation of shikimic acid resulting from the action of glyphosate may represent an expressive drain of carbon in Calvin cycle, by redirecting erythrose-4- phosphate, severally reducing photosynthetic rate. Fuchs et al. (2002) also showed a drastic reduction in carbon dioxide assimilation after glyphosate application in Abuthilon teophrasti. Figure 2. Electron transport rate (ETR) of mature leaves from maize plants submitted to glyphosate and phosphite application associated and isolated. In the early hours after treatments application (2 HAA), there was an increase in ETR of young leaves when comparing with the check/control (Figure 3). In Figure 4 it is possible to observe an increase in ETR of mature leaves, five hours after treatments application. Cedergreen and Olesen (2010) observed increased in photosynthetic rate of barley plants subjected to 22 and 45 g ha-1 doses just a few hours after application. This effect on ETR may be related to an increase in the growth of these plants (Table 2) because second these authors, to achieve an increase in dry mass that ranges from 10 to 25% in one week or more, plants should increase photosynthetic rate or decrease respiration rate in response to low doses of glyphosate. However the physiological mechanism involved in this apparent process of growth change is currently unknown. 0 20 40 60 80 100 120 140 0 50 100 150 200 250 300 350 ET R (% in re la tio n to th e ch ec k) Hours after application (HAA) Glyphosate 72 g ha-1 Glyphosate 720 g ha-1 Glyphosate 72 g ha-1 + Phosphite Glyphosate 720 g ha-1 + Phosphite Phosphite Gomes et al. 84 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 Figure 3. Electron transport rate (ETR) of young leaves from maize plants submitted to glyphosate and phosphite application associated and isolated until 32 hours after application (HAA). Figure 4. Electron transport rate (ETR) of mature leaves from maize plants submitted to glyphosate and phosphite application associated and isolated until 32 hours after application (HAA). In the treatments that glyphosate was applied on the lowest dose, there was no ETR reduction, and in most evaluation periods, these plants showed bigger ETR than check/control without application. Gravena et al. (2009) reported that photosynthesis of lemon clove was little affected by application glyphosate doses (180, 360 and 720 g ha-1) and that chlorophyll levels increased during the evaluation period and were not affected by the herbicide. CO2 assimilation rate followed by chlorophyll content showed a tendency to increase daily, without significant effect of herbicide. Potassium phosphite applied alone did not cause toxic effect on maize plants. Maize plants subjected to 72 g ha-1 dose of isolated potassium phosphite, presented higher growth 0 20 40 60 80 100 120 140 0 5 10 15 20 25 30 35 ET R (% in re la tio n to th e ch ec k) Hours after application (HAA) Glyphosate 72 g ha-1 Glyphosate 720 g ha-1 Glyphosate 72 g ha-1 + Phosphite Glyphosate 720 g ha-1 + Phosphite Phosphite 0 20 40 60 80 100 120 140 0 5 10 15 20 25 30 35 ET R (% in re la tio n to th e ch ec k) Hours after application (HAA) Glyphosate 72 g ha-1 Glyphosate 720 g ha-1 Glyphosate 72 g ha-1 + Phosphite Glyphosate 720 g ha-1 + Phosphite Phosphite Gomes et al. 85 Rev. Bras. Herb., v.12, n.1, p.78-86, jan./abr. 2013 than control (without glyphosate application). When associated with the highest glyphosate dose (720 g ha-1), phosphite was responsible for reducing plants toxicity and ETR increase, when compared with the application of isolated glyphosate. Electron transport rate showed an increase in treated plants a few hours after glyphosate application associated or not to phosphite, and during later evaluating periods, only plants subjected to higher glyphosate dose (720 g ha-1) in association or not with potassium phosphite, was observed an ETR reduction in young leaves first, and subsequently in the mature ones. This increase in photosynthetic efficiency already in early hours may be related with growth stimulus effect on the lowest dose. 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