Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1217 Vol.53, n. 5: pp. 1217-1224, September-October 2010 ISSN 1516-8913 Printed in Brazil BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY A N I N T E R N A T I O N A L J O U R N A L Evaluation of the Use of Vinasse as a Biostimulation Agent for the Biodegradation of Oily Sludge in Soil Sérgio Henrique Rezende Crivelaro1, Adriano Pinto Mariano1* Luis Tadeu Furlan2, Renato Almeida Gonçalves2, Paulo Negrais Seabra3 and Dejanira de Franceschi de Angelis1 1Departamento de Bioquímica e Microbiologia; Instituto de Biociências; Universidade Estadual Paulista; Av. 24-A; 1515; C. P.: 199; 13506-900; Rio Claro - SP - Brasil. 2Refinaria de Paulínia-REPLAN (Petrobras); Paulínia - SP - Brasil. 3Centro de Pesquisas e Desenvolvimento Leopoldo Américo Miguez de Mello (Petrobras); Rio de Janeiro – RJ - Brasil ABSTRACT This work aimed to study the effect of vinasse on the biodegradation in soil of oily sludge from the Replan-Petrobras oil refinery. The Bartha respirometric method was used to verify the efficiency of the treatments with soil, oily sludge (7 and 14% m/m) and soil moisture adjustment with or without vinasse (0.11 mL/g dry soil) for 121 days. Although an increase in the soil microbial population was obtained with the vinasse, it demonstrated not to be adequate to enhance the biodegradation efficiency of the oily sludge in soil, since there was no difference between the CO2 produced from treatments with or without vinasse after the complete consumption of the vinasse. Thus, the use of vinasse as an amendment to biodegradation processes showed not to be efficient under the studied conditions. Key words: biodegradation, soil, oily sludge, vinasse, landfarming *Author for correspondence: adrianomariano@yahoo.com.br INTRODUCTION The oil industry is responsible for the generation of high amounts of oily and viscous residues, which are formed during the production, transportation and refining. Such residues, called oily sludge, are basically composed of oil, water, solids, and their characteristics, such as varied composition, make their reutilisation very difficult, and confer on them high recalcitrance. Moreover, incineration of this residue is not recommended due to high energy costs, the potential risk of air pollution and the persistence of PAHs (Ururahy et al., 1998). Thus studies must be carried out aiming the improvement of the current suitable technologies or the development of new ones. The landfarming system is the most world-wide known technology used to treat the oily sludge. This system is designed to ensure the aerobic biodegradation of the residue in the soil superficial layer (Kataoka, 2001). However, this technology demands long process times and large areas and there is a potential risk of groundwater contamination if the landfarming is not well constructed. Due to the low biodegradation efficiency observed in the treatments where the oily sludge is mixed with soil (landfarming, biopile, and composting) Crivelaro, S. H. R. et al. Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1218 (Bossert et al., 1984; Mishra et al., 2001; Juteau et al., 2003; Ayotamuno et al., 2007; Machín- Ramírez et al., 2008), nutritional amendment is one of the possibilities to tackle this problem (Brown1 Hahn and Loehr, 1992; Chokshi and Nelson, 2003; Mrayyan and Battikhi, 2004). According to Ururahy et al. (1998), oily sludges possess limited amounts of nitrogen and phosphate, and most of the nitrogen is not available, since it is part of complex structures, relatively inaccessible to microorganisms. In Brazil, it is very common to dispose vinasse (a by-product of the alcohol distillation process with fertilisation characteristics) in agricultural fields, a process called ferti-irrigation, as a partial or total substitute for mineral fertilisers, mainly potassium. In general, to produce one litre of alcohol, 10 to 15 litres of vinasse are produced, depending on sugar cane quality and the industrial process (Cortez et al., 1992), which corresponds to approximately 170 billion litres per year of this residue in Brazil (Agrianual, 2004). Considering that the ethanol production in Brazil is in expansion due to a rising internal and world- wide demand for alternative energy sources and that soils have a limited support capability to receive the vinasse, the search for additional application for the vinasse is very important. Thus, this work evaluated the potential use of vinasse as a biostimulation agent for the biodegradation of oily sludge in soil. MATERIALS AND METHODS Oily sludge and vinasse characteristics The oily sludge was obtained from Replan/Petrobras oil refinery (Paulinia/SP/Brazil) prior to its disposal in the landfarming system. Vinasse was collected at Usina América (Assis/SP/Brazil). Tables 1 and 2 show the physicochemical characteristics of the oily sludge and vinasse, respectively. The analyses were performed by Icasa laboratory (Instituto Campineiro de Análise de Solo e Adubo – Icasa), according to the methodology proposed by Embrapa (1997). Table 1 - Oily sludge characteristics. N % 0.53 total P (P2O5) % 0.0908 K K2O (%) 0.025 Ca % 0.29 Mg % 0.11 S % 0.32 Fe % 0.69 Mn % 0.0138 Cu % 0.00275 Zn % 0.0683 B % 0.005 Na % 0.13 Co % 2.50 Mo % -a Al % 0.71 Ba % 0.005211 Cd % 0.000028 Cr % 0.005781 Ni % 0.001414 Pb % 0.001323 moisture 65oC % 80.86 moisture 105oC % 81.33 organic matter % 63.85 ashes % 36.15 density g/mL 1.03 C/N ratio 66.93 pH 7.20 a not detected. Evaluation of the Use of Vinasse as a Biostimulation Agent Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1219 Table 2 - Vinasse characteristics. N % 0.18 B % 0.0015 total P (P2O5) % 0.009 Na % 0.01 K K2O % 0.48 Co % -a Ca % 0.13 Mo % -a Mg % 0.0388 Al % 0.0025 S % 0.12 organic matter % 2.88 Fe % 0.00725 C/N ratio 8.89 Mn % 0.00075 density g/mL 1.01 Cu % -a pH 4.24 Zn % 0.0005 a not detected. Soil respirometric experiment The soil sample was collected from a loan area at a landfill in Rio Claro/SP/Brazil. Until performing the biodegradation experiment, the samples were stored at 5 oC. Table 3 summarises some physicochemical characteristics of the soil sample. The physicochemical analyses were performed by Icasa laboratory according to the methodology proposed by Embrapa (1997). In order to verify the influence of the vinasse in the biodegradation of the oily sludge, a batch biodegradation experiment (121 days) was carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production (Bartha and Pramer, 1965; Régis and Bidoia, 2005; Inazaki et al., 2004; Mariano et al., 2008a-c and 2009b). Table 4 shows the treatments simulated in the respirometric experiments. The quantity of water or vinasse added to adjust the soil moisture to 64.2% of the soil field capacity was 0.11 mL/g dry soil. For each treatment, the biometer flasks were prepared in triplicates (3 x 50 g of soil) and incubated at 27 oC in the dark. The CO2 produced was trapped in a 10.0 mL solution of KOH (0.2 N), located in the side-arm of the biometer. This solution was periodically withdrawn by syringe, and the amount of carbon dioxide absorbed was then measured by titrating the residual KOH [after the addition of barium chloride solution (1 mL; 1.0 N) used to precipitate the carbonate ions] with a standard solution of HCl (0.1 N). During this procedure, the biometers were aerated for 1.5 minute through the ascarite filters. At the end of the experiment, replicates of each treatment were thoroughly mixed together for microbiological analysis. Table 3 - Soil characteristics. (mmolc/dm3) pH (CaCl2) 4.1 Ca 8.0 organic matter (%) 0.5 Mg 5.0 P (%) 0.0003 Al 21.0 moisture (%) 12.0 K 0.6 field capacity (g H2O/g soil) 0.36 grain size distribution (%) sand 47.2 silt 22.7 clay 30.1 micronutrients (%) S Na Fe Mn Cu Zn B 0.001 0.0007 0.0004 0.00011 0.00001 0.00007 0.000048 Crivelaro, S. H. R. et al. Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1220 Table 4 - Respirometric experiments. treatment experimental condition 1 soil + water 2 soil + vinasse 3 soil + oily sludge (7% m/m) + water 4 soil + oily sludge (7% m/m) + vinasse 5 soil + oily sludge (14% m/m) + water 6 soil + oily sludge (14% m/m) + vinasse Total heterotrophic bacteria and fungi were counted by using the pour plate technique on Plate Count Agar (PCA) and Potato Dextrose Agar (PDA + plus antibiotic), respectively. The plate counts were performed as follows: samples of 1 g of soil were added to 9 mL of 0.85 % sterile saline solution in test tubes and agitated mechanically for 2 minutes. After appropriate serial dilutions, 1 mL of the suspension were spread over the surface of duplicate petri dishes and incubated for 48 h at 35 oC (bacteria) or 28 oC (fungi). The total heterotrophic bacteria count was carried out at the beginning and at end of the respirometric experiment. RESULTS AND DISCUSSION The daily CO2 productions in the respirometric experiment are represented in Figures 1 to 3, where each error bar represents one SD (standard deviation) of three replicates. The evolution of the CO2 production in treatment 2 (soil + vinasse) (Fig. 1) showed that the vinasse was practically totally biodegraded in approximately 40 days after being applied to the soil. When considering the treatments with oily sludge and vinasse (Fig.2 and 3), a similar behaviour was observed, i.e. the CO2 production in the treatments with vinasse initially differentiate from the controls and after similar period (40 days), it decreased until reaching values next to the controls. Thus, since there was no difference between the CO2 produced from the treatments with or without vinasse after the complete consumption of the vinasse, it showed that the vinasse did not biostimulate the biodegradation of the oily sludge at the mineralization level. It is important to emphasise that mineralization studies involving measurements of total CO2 production can provide excellent information on the biodegradability potential of hydrocarbons (Balba et al., 1998). 0 50 100 150 200 250 300 350 0 20 40 60 80 100 120 time (days) C O 2 p ro d u ce d ( µ m o l.d -1 ) (1) - soil (2) - soil + vinasse Figure 1 - CO2 production for treatments 1 and 2. Evaluation of the Use of Vinasse as a Biostimulation Agent Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1221 0 50 100 150 200 250 300 350 400 0 20 40 60 80 100 120 time (days) C O 2 p ro d u ce d ( µ m o l.d -1 ) (3) - soil + oily sludge 7% (4) - soil + oily sludge 7% + vinasse Figure 2 - CO2 production for treatments 3 and 4. 0 50 100 150 200 250 300 350 400 0 20 40 60 80 100 120 time (days) C O 2 p ro d u ce d ( µ m o l.d -1 ) (5) - soil + oily sludge 14% (6) - soil + oily sludge 14% + vinasse Figure 3 - CO2 production for treatments 5 and 6. The microorganisms (total heterotrophic bacteria and fungi) counts carried out at the initial and final times of the respirometric experiment are shown in Figures 4 and 5. In almost all counts, the treatments with vinasse had a microbial population greater at the final time than the controls without vinasse. The exception was verified in the total heterotrophic bacteria count for treatments with an oily sludge concentration of 7 % (treatments 3 and 4, Fig. 5). It indicated that generally vinasse had a positive effect on the soil microbial population, overcoming the toxicity of the oily sludge that could have negative effects on the microbiot, as observed by Mielniczuk (1991). The increase in the soil microbial population caused by the vinasse was also observed by Prata et al. (2001) and Mariano et al. (2009a) in their biodegradation experiments. However, as observed by Hickman and Novak (1989), the total microbial biomass could be a poor predictor for determining the biodegradation potential, mainly because the active biomass could differ in species composition and in metabolic regimes. Certainly these additional microorganisms preferentially biodegraded the more labile carbon sources as the glycerol present in the vinasse (Rodella et al., 1983; Prata et al., 2001); otherwise, a marked decrease in the CO2 production after the consumption of the vinasse would not be observed. Crivelaro, S. H. R. et al. Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1222 0 500 1000 1500 2000 2500 3000 1 2 3 4 5 6 treatment C F U / (g d ry s o il) x 1 04 Initial time Final time Figure 4 - Total heterotrophic bacteria at initial and final time of the treatments. 0 100 200 300 400 500 600 700 800 900 1 2 3 4 5 6 treatment C F U / (g d ry s oi l) x 1 04 Initial time Final time Figure 5 - Total fungi at initial and final time of the treatments. . CONCLUSIONS Although an increase in the soil microbial population was obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of the oily sludge at the mineralization level. Thus, the use of vinasse as an amendment to biodegradation processes showed not to be efficient under the studied conditions. ACKOWLEDGMENTS The authors acknowledge Agência Nacional do Petróleo, Gás Natural e Biocombustíveis (ANP) (PRH-05) for the financial support, and REPLAN/PETROBRAS and Usina América for supplying oily sludge and vinasse, respectively. Evaluation of the Use of Vinasse as a Biostimulation Agent Braz. Arch. Biol. Technol. v.53 n.5: pp. 1217-1224, Sept/Oct 2010 1223 RESUMO Este trabalho objetivou estudar o efeito da vinhaça na biodegradação em solo da borra oleosa proveniente da refinaria de petróleo Replan- Petrobras. Foi utilizado o método respirométrico de Bartha para verificar a eficiência de tratamentos constituídos de solo, borra oleosa nas concentrações 7 e 14 % (m/m) e ajuste da umidade do solo com e sem vinhaça (0,11 mL/g solo seco) durante 121 dias. 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