Journal of Agricultural Science; Vol. 6, No. 6; 2014 ISSN 1916-9752 E-ISSN 1916-9760 Published by Canadian Center of Science and Education 54 Animal Performance, Feeding Behaviour and Carcass Traits of Feedlot Cattle Diet Fed With Agro-Industrial By-Product as Fat Source Angelo Polizel Neto1, Roberto O. Roça2, Renata H. Branco3, Sarah M. F. Bonilha3, Ernani N. Andrade2, Tatiane L. S. Corvino3 & Helen F. B. Gomes1 1 Instituto de Ciências da Saúde, Universidade Federal do Mato Grosso, Sinop, Mato Grosso, Brazil 2 Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agronômicas, Departamento de Economia, Sociologia e Tecnologia, Botucatu, São Paulo, Brazil 3 Instituto de Zootecnia, Centro de Pesquisas em Pecuária de Corte, Sertãozinho, SP, Brazil Correspondence: Angelo Polizel Neto, Instituto de Ciências da Saúde, Universidade Federal do Mato Grosso, Avenida Alexandre Ferronato, 1200, Sinop, Mato Grosso, Brazil. E-mail: polizelneto@ufmt.br Received: February 21, 2014 Accepted: March 25, 2014 Online Published: May 15, 2014 doi:10.5539/jas.v6n6p54 URL: http://dx.doi.org/10.5539/jas.v6n6p54 Abstract In feedlot system is particularly important to reduce the cattle feeding cost without impact on the animal gain and carcass, in this sense, aimed with this study to evaluate animal performance and carcass traits of the young Nellore male (n = 40) finished with agro-industrial by-products in feedlot diet. The addition of cottonseed by-product (CSB) was based on the ether extract (EE) contents in the feedlot diet: 3, 4 and 5%; and two other reference treatments were also tested, with 3 and 5% of EE content and soybean by-product (SOB) as fat source, totalling five experimental diets. In diets with 3% EE, CSB did not alter performance, gain cost or carcass traits compared to the SOB. In diets with 5% EE, animals fed with CSB showed greater dry matter (DM) intake than animals fed with SOB (10.34 versus 8.94 kg/day), but because CSB is a cheaper ingredient than SOB, it reduced the gain cost from 1.60 to 1.35 US$/kg. The CBS used in diet with 3, 4 and 5% EE increased the daily gain (1.17, 1.38 and 1.50 kg/day) and the rumination time (225, 338 and 370 min/day, respectively). So, CSB does not change the carcass traits nor the feeding behaviour when compared to SOB. The increased of CSB concentration in the diet raised the daily gain, DM intake and rumination time, with no changes in carcass traits. Keywords: behaviour, cottonseed by-product, fat source, gain cost 1. Introduction Cattle feedlot finishing faces challenges imposed by feeding cost minimization, which is most of the costs, and the growing demand for a rational and sustainable use of natural resources. One way to achieve this cost minimization is replacing traditional grains used in the animal feeding by other protein and energy sources presenting lower cost and not competing with the ingredients used in foods for human consumption. Millen, Pacheco, Arrigoni, Galyean, and Vasconcelos (2009) reported that 80% of the Brazilian feedlot consulting nutritionists used a by-product in their formulations for beef cattle, especially cottonseed. A similar study conducted in the United States showed that 82% of Americans nutritionists also use some by-product in their formulations, mainly corn derived from ethanol distillers (Vasconcelos & Galyean, 2007). Based on modifications of the process of ethanol production, Berger and Singh (2010) predicted effects in animal nutrition, bearing in mind the amount and nutritional characteristics of the new by-products. They also suggested that beef cattle can efficiently use these by-products as replacements for the traditional sources of protein and energy. Similar results were obtained by Wogar and Ayuk (2012) with grasscutter in Nigeria farms fed with low-cost agro-industrial by-products. The extraction of oil from cottonseed aims at obtaining vegetable oil widely used in the food industry and increasingly in biodiesel production (Meneghetti et al., 2006). Winterholler, Lalman, Hudson, and Goad (2009) reported that some industries have developed mechanical techniques for cottonseed oil extraction because of high demand for cottonseed oil as a raw material for biofuel production, generating cottonseed meal extruded and pelleted as by-product, which can be used in animal feeding. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 55 Studies have been conducted for the feasibility of the use of cotton by-products in ruminant feeding, especially cottonseeds (Aferri, Leme, Silva, Putrino & Pereira, 2005; Stelzleni, Froetschel, & Pringle, 2013) cottonseed meal, (Pina et al., 2006) cottonseed hulls (Magalhães et al., 2005; Kazama et al., 2008) and cottonseed cake for dairy cows (Alves et al., 2010); and wheat offal and soybean meal to grasscutters (Wogar & Ayuk, 2012). However, differences in dry matter (DM) intake related to fat sources using cottonseed and its by-products were also observed (Cranston et al., 2006). Furthermore Costa et al. (2011) found a linear decrease in DM intake and weight gain of cattle with increase of cottonseed levels; or Gunn, Weaver, Lemenager, Gerrard, Claeys, and Lake (2009), who found a reduction in gain of Angus cattle fed with distillery waste and soybean oil. Thus, the objective of this study was to evaluate animal performance, feeding behaviour and carcass traits of young Nellore bulls finished in feedlots with increasing of agro-industrial by-products in the diet. 2. Methods 2.1 Animal Welfare, Pens and Management All procedures in this trial were approved by the Ethics Committee of Animal Experimentation of UNESP “Julio de Mesquita Filho”, Campus of Botucatu – Sao Paulo, Brazil (48/2008-CEEA). The experiment was carried out in the Advanced Center of Technological Research of Beef Cattle Agribusiness, a sector in the Institute of Animal Science, linked to the Agriculture and Supply Secretary of São Paulo State, located in Sertaozinho, Sao Paulo, Brazil, latitude 21º10′S and longitude 48º05′W. This region has humid tropical climate, with annual average temperature of 24ºC and annual average rainfall of 1,300 mm. Forty 20±2 month-old Nellore bulls were assigned to eight different plots according to their initial body weight (BW) (313.8±41.2 kg) before their adaptation to diets. The animals were housed in individual pens, so that each animal was considered an experimental unit. Their adaptation to diets and pens lasted 22 days, and they were evaluated during a 102 days experimental period. The 10 m2 area pens were partially covered and had concrete floor, with an individual 1.5 meter linear trough and a 100 litre Australian water trough between every two pens. The animals were individually identified by a tattoo on the left ear, treated against endoparasites and ectoparasites, and vaccinated against clostridiosis (Clostridium sp; Ourovac® Clostridium; Ourofino, Cravinhos, Brazil) and foot-and-mouth disease. 2.2 Treatments The two agro-industrial by-products used were cottonseed by-product (CSB) and soybean by-product (SOB), and the feedlot diets were formulated according to the nutritional demands estimated by CNCPS v. 6.1 – Cornell Net Carbohydrate and Protein System (Fox, Sniffen, O'Connor, Russell, & Van Soest, 1992) The addition of CSB was based on the ether extract (EE) contents in the feedlot diet: 3, 4 and 5%. Two other reference treatments were also tested, namely 3 and 5% of EE content and SOB as fat source, totalling five experimental diets, presented in Table 1 and abbreviated as: 3SOB = 3% of ether extract in the diet with fat source from soybean by-product; 5SOB = 5% of ether extract in the diet with fat source from soybean by-product; 3CSB = 3% of ether extract in the diet with fat source from cottonseed by-product; 4CSB = 4% of ether extract in the diet with fat source from cottonseed by-product; 5CSB = 5% of ether extract in the diet with fat source from cottonseed by-product. The CSB utilized, provided by Bunge Alimentos Ltda, and is the resulting by-product of the physical extraction of semi-delinted cottonseed oil, similar to the by-product described by Winterholler et al. (2009). In the SOB diets, acidic soybean dreg was used as fat source, which is a residual by-product of the refining process of commercial soybean oil. Protein diet values were extrapolated so that they provided greater CSB inclusion, and, therefore, the diets were formulated with contents close to 18% CP, keeping the concentrate: roughage ratio close to 80:20, including 31.5% of corn. In diets with the same EE content, similar amounts of urea and molasses were used. The ration was provided as total diet, twice a day, at 08:00 and 16:00, in amounts that allowed leftovers between 5 and 10% of the observed DM voluntary intake, as a way to allow animals to express the consumption. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 56 Table 1. Composition of diets provided to feedlot animals (%DM) Ingredients Diets+ 3SOB 5SOB 3CSB 4CSB 5CSB Brachiaria sp hay 19.5 19.9 19.5 19.4 19.4 Ground corn 31.2 31.9 31.2 31.1 31.1 Ground sorghum 35.2 14.7 33.02 19.31 5.52 Soybean meal (46% CP) 5.84 24.1 - - - Soybean by-product (SOB) 1.66 7.43 - - - Cottonseed by-product (CSB) ++ - - 9.77 26.1 42.2 Molasses 2.63 0.34 2.63 1.55 0.33 Urea 2.65 0.32 2.67 1.47 0.31 Mineral supplement+++ 1.10 1.10 1.10 1.10 1.10 Feed cost (U$ kg-1 dry matter) 0.212 0.261 0.196 0.195 0.193 Chemical Analysis Total digestible nutrients 66.1 68.7 64.2 64.4 64.5 Metabolizable energy (Mcal/kg) 2.39 2.43 2.32 2.33 2.34 Crude protein 17.9 18.9 17.7 18.6 19.4 Ether extract 3.07 5.00 3.01 3.86 4.79 Neutral detergent fiber 40.6 40.1 45.8 45.2 49.1 Lignin 2.07 3.34 2.26 4.00 5.57 +3SOB and 5SOB = diets with 3 and 5% ether extract and soybean by-product as lipid source; 3CSB, 4CSB and 5CSB = diets with 3, 4 and 5% ether extract and cottonseed by-product as lipid source, respectively. ++ CSB composition: 91.2% DM; 9.33% EE; 28.5% CP; 51.7% NDF; 31.9% ADF; gossypol content was under the minimum limit of analytical quantification (5 mg/kg). SOB composition: 56.3% DM and 38.4% of EE. +++Composition of the mineral supplement (amount/kg): 180 g Ca; 90 g P; 10 g Mg, 13 g S; 93 g Na; 145 g Cl; 17 mg Se; 1000 mg Cu; 826 mg Fe; 4000 mg Zn; 1500 mg Mn; 150 mg I; 80 mg Co; 900 mg Fl; 38.7 ppm Monensine. 2.3 Performance The adaptation period lasted 22 days, with the initial supply of diets around 2.3% BW, and following the protocol of sequential reduction in roughage:concentrate ratio: 5 days with 50:50; 5 days with 40:60; 5 days with 30:70 and 7 days with 20:80. At the end of the adaptation period the animals were re-weighed, and this weight was considered as the weight at the onset of the experiment (initial BW). The animals were weighed in subperiods: after 28, 56, 84 and 102 days from the beginning of the experiment; all weighing were made a 14 hours solid fasting with free access all time to water. Daily were weighed the diet offered and the leftovers to determination of feed intake. Representative samples of diets and leftovers for individual animals were collected weekly, forming a composite sample of each diet and other individual leftovers in each subperiod. Diets and leftovers were dried in a forced-air ventilation oven 55 ± 5ºC to constant weight and they were ground in a Stationary Mill type Willey, using 1 mm sieves. The dry matter (item 934.01), ether extract (item 920.39), crude proteins by the micro-Kjeldahl method (item 954.01) were determined according to Association Official Analysis Chemistry (2007). The levels of lignin, acid and neutral detergent fiber were measured according to Van Soest, Robertson and Lewis (1991). 2.4 Feeding Behaviour Feeding behaviour was observed for 3 days at random on days 49th, 65th and 96th, during an uninterrupted period of 24 hours, in 10-minute intervals, through direct and individual observation, with previous adaptation of the animals, to determine the time spent in feeding and rumination, as well as the time in which the animal remained lying. During the night observation, the environment was maintained with artificial illumination, also with previous adaptation of the animals. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 57 Feeding and rumination times were adjusted to the average DM intake observed on two consecutive days: the day before and the day of behaviour observation. This procedure was performed to adjust the feeding time to intake, because the beginning of the observation did not always coincide with the exact time at which the diet was served to the animals, and the time spent on rumination also reflected the intake of the previous day. 2.5 Slaughter and Carcass Evaluation The animals were slaughtered at Frigonobre Slaughterhouse in Torrinha, SP, Brazil, 204 km from the experiment location. All animals were slaughtered in a single day in the same lot. At exsanguination, the animals were identified by their tattoos and numbered in ordinal order according to their entrance. The animals were stunned by a pneumatic hammer, and immediately after the procedures of exsanguination, skinning, evisceration and carcass preparation for chilling were done. At evisceration, the liver and kidney, pelvic and heart fat (KPH) were collected and weighed. After the cooling period (24 hours at 7ºC), the beef carcass side was re-weighed to obtain the cold carcass weight. Next, the internal length of half carcasses, the distance between the cranial extremity of the iliac tuberosity and the cranial border of the first rib were all measured. The half carcasses were divided into three primary cuts: forequarter, composed of the cranial portion of the cross section between the 5th and 6th ribs; spare ribs, composed of the latero-ventral part of the abdomen and the 6th to 13th ribs, dorsally sectioned at ± 20 cm from the midline of the half-carcass, and the hindquarter, comprised by the full leg and all loin from the 6th rib. The Musculus longissimus (LM), as well as its bone base was sectioned between the 12th and 13th ribs to enable the measurement of both the subcutaneous fat thickness, with the aid of digital callipers, and the LM area, by vegetal paper tracing, for further evaluation on a digital table with planimetry system, in the Laboratory of Remote Sensing in the Department of Rural Engineering - FCA, UNESP – Botucatu. The proportion of the whole carcass meat cuts was calculated by the equation proposed by Cross, Carpenter & Smith (1973), currently accepted by the United States Department of Agriculture: Cutability = 51.34 – 2.277 x SBF – 0.462 x KPH + 0.1147 x LMarea – 0.0205 x HCW (1) where: SBF=subcutaneous fat thickness (cm); KPH = kidney, pelvic and heart fat (percentage of HCW); LMarea = M. longissimus area (cm2); HCW = hot carcass weight (kg). 2.6 Statistical Analysis Data on final BW, feeding behaviour and carcass traits were evaluated with animal (random), diet and plot effects (fixed) with initial BW as co-variable; data on performance, nutrients and DM intake were added sub-period experimental effect as repeated measures. Means were tested by four contrasts, the first two aimed to analyse if, for the same dietary EE content, CSB differed from SOB: C1 = 3SOB versus 3CSB and C2 = 5SOB versus 5CSB; the other two contrasts (C3 and C4) aimed to evaluate the linear and quadratic effect of CSB inclusion. All data were analysed using PROC MIXED by Statistical Analysis System Version 9.0 (SAS, 2002) and the contrasts were tested by Scheffé’s test, considering the difference to be significant when P< 0.05. Moreover, when significant at C3 and/or C4 the CSB increase was evaluated by PROC REG. 3. Results and Discussion 3.1 Performance The fat sources, SOB or CSB at 3 and 5 % of EE in the diet, did not affect (P ≥ 0.05) the final BW and the daily gain (Table 2; C1 and C2, respectively), which is in agreement with Lofgreen (1965) that studied cattle fed residual oil from restaurants and cottonseed, with Zinn (1989a) who compared combinations of restaurant waste oil, animal fat and vegetable in the diet of feedlot cattle, with Cranston et al. (2006) who provided the feedlot cattle diets with cottonseed and cottonseed by-products, and all studies did not find difference in beef cattle weight gain. In addition, this finding was similar to that of Stelzleni et al. (2013), who found no change in performance and DM intake in heifers supplemented with full-fat extruded cottonseed pellets. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 58 Table 2. Estimated means of performance, intake and gain of Nellore cattle fed with agro-industrial by-products Component Means Treatments+ SEM++ P+++ 3SOB 5SOB 3CSB 4CSB 5CSB Model C1 C2 C3 C4 Initial BW (kg) 314 309 319 308 313 319 6.32 0.01 - - - - Final BW (kg) 452 440 464 433 454 467 7.65 0.03 0.57 0.83 0.02(2) 0.69 Daily gain(kg/day) 1.35 1.24 1.47 1.17 1.38 1.50 0.10 0.03 0.57 0.83 0.02(3) 0.69 DM intake(kg/day) 9.20 8.43 8.94 8.34 9.90 10.3 0.23 0.01 0.89 0.04 0.01(4) 0.36 DM/BW (%) 2.41 2.27 2.30 2.24 2.58 2.65 0.05 0.01 0.85 0.02 0.02(5) 0.29 Gain:Feed (g/kg) 161 162 179 154 154 157 0.01 0.03 0.31 0.01 0.42 0.78 Gain Cost++++ (US$/kg gain) 1.45 1.45 1.60 1.46 1.41 1.35 0.10 0.04 0.98 0.03 0.14 0.92 Nutrients Daily Intake CP intake (kg/day) 1.56 1.40 1.56 1.29 1.68 1.85 0.05 0.00 0.32 0.02 0.00(6) 0.25 CP intake/BW (%) 0.41 0.38 0.40 0.35 0.44 0.47 0.01 0.00 0.21 0.01 0.00(7) 0.17 EE intake (kg/day) 0.31 0.26 0.36 0.24 0.34 0.37 0.01 0.00 0.34 0.88 0.00(8) 0.06 EE intake/BW (%) 0.08 0.07 0.09 0.06 0.09 0.09 0.002 0.00 0.16 0.93 0.00(9) 0.02 NDF intake (kg/day) 6.31 6.62 5.81 6.83 6.40 5.81 0.11 0.01 0.47 0.99 0.12 0.94 NDF intake/BW (%) 1.66 1.78 1.50 1.84 1.67 1.50 0.02 0.02 0.35 0.97 0.08 0.93 +3SOB and 5SOB = diets with 3 and 5% ether extract and soybean by-product as lipid source; 3CSB, 4CSB and 5CSB = diets with 3, 4 and 5% ether extract and cottonseed by-product as lipid source, respectively. ++SEM = standard error of the mean, n=40. +++C1 = 3SOB vs 3CSB, C2 = 5SOB vs 5CSB, C3 = linear effect of CBS inclusion, C4 = quadratic effect of CBS inclusion, contrasts tested by Scheffé’s test. ++++Gain Cost, calculated by equation = [(Dry Matter Intake x Diet Costs)/Daily Gain] BW = body weight; DM = dry matter; CP = crude protein; EE = ether extract; NDF = fiber in neuter detergent Final BW = 79.53 + 1.07 x Initial BW + 9.13 x EE of the diet with CSB (R2=0.69; P<0.001) (2) Daily gain = 0.99 + 0.093 x EE of the diet with CSB (R2=0.07; P=0.22) (3) DM intake = 1.65 + 0.018 x Initial BW + 0.534 x EE of the diet with CSB (R2=0.30; P=0.03) (4) DM intake/BW = 2.09 + 0.1 x EE of the diet with CSB (R2=0.07; P=0.21) (5) CP intake = -0.26 + 0.003 x Initial BW + 0.21 x EE of the diet with CSB (R2=0.53; P<0.001) (6) CP intake/BW = 0.28 - 2.1x10-4 x Initial BW + 0.05 x EE of the diet with CSB (R2=0.42; P=0.002) (7) EE intake = -0.07 + 6.4x10-4 x Initial BW + 0.046 x EE of the diet with CSB (R2=0.51; P<0.001) (8) EE intake/BW = 0.038 + 0.01 x EE of the diet with CSB (R2=0.40; P<0.001) (9) However, the CSB increase in the diet changed (P < 0.05) the final BW and daily gain, in that animals showed 1.17 ± 0.14 kg/day of the gain when CSB was used 3% EE diet and 1.50 ± 0.19 kg/days in 5% EE diet, which is an increase superior to 28%. An opposite finding was reported by Gunn, Weaver, Lemenager, Gerrard, Claeys, and Lake (2009), who found a reduction in gain of Angus cattle with increase the diet EE, using distillery waste and soybean oil as fat source. The difference in C3 for final BW and the non-significance in C4 for the same variable indicates that the mean in the intermediate level of CSB inclusion is not different (P ≥ 0.05) from the mean of the extreme levels, indicating a linear relationship between the final live weight with increasing inclusion of CSB, confirmed by the multiple linear regression [equation 2], in which the final BW of 433 ± 20, 454 ± 16 and 467 ± 16 kg were found for an increase of 3, 4 and 5% of EE dietary content with CSB as source. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 59 Despite the significant contrasts that were detected for daily gain, as well as for the final BW, the daily weight gain data did not fit the proposed linear equation (P = 0.22) [Equation 3]. Therefore, the linearity of the gain as a function of the increasing content of CSB in the diet cannot be stated. A similar conclusion was reported by Vander Pol, Luebbe, Crawford, Erickson, and Klopfenstein (2009) who provided confined cattle with corn by-product from distillers in increasing levels of EE dietary (4, 6.4 and 8.8%) and found no changes in animal performance. The ratio of DM intake by BW was 2.41 ± 0.05, which was close to the value of 2.55% found by Machado Neto et al. (2010), for Nellore finished in feedlot. Ítavo, Valadares Filho, Silva, Valadares, and Cecon (2002), when studying Nellore fed with different levels of dietary protein, found the proportion of 2.21% for diets with levels 18% of protein. However, the average DM intake found in this study was substantially higher than the mean value of 1.9% shown by Messana, Berchielli and Arcuri (2010) with cannulated Nellore animals fed 80% DM of cottonseed processed residue. In addition, those authors found that intake might have been affected by the high lignin amount in the fibrous portion of that dietary ingredient. In 3% EE diets, the use of fat sources did not affect (P = 0.89) the DM intake of animals, which were found to be 8.43 ± 0.49 and 8.34 ± 0.56 kg for SOB and CSB, respectively. However, in the 5% EE diets, a greater DM intake was found, in kg/day (P = 0.04) and proportion to BW (P = 0.02). Animals had a 1.42 kg greater of DM intake for the CSB diet, which significantly reduced feed efficiency of 179 ± 0.01 g/kg for 5SOB to 157 ± 0.01 g/kg for 5CSB. This means that even in the diets with 42% of CSB (5CSB diet; Table 1) DM intake was not affected by the inclusion of this by-product, as reported in Messana et al. (2010). The occurrence of increased DM intake and reduced feed efficiency with the use of CSB can be attributed to the lower energy intake of this ingredient (Table 1), which has possibly been compensated by a greater intake by the animals, because the energy density of the diet is an important intake regulatory factor (Zinn, 1989a). This interpretation is corroborated by the study of Galyean and Defoor (2003), who stated that bovine fed diets with lower energy might raise intake to achieve the same energy level as long as the intake is not limited by the filling of the digestive tract. Differences in DM intake related to fat sources were also observed by Cranston et al. (2006), using cottonseed and its by-products, and the same authors reported that the increase in DM intake in diets with cottonseed and its by-products may be the effect of greater fiber and lower energy concentration of these diets. Regarding the dietary volume effect of CSB, a by-product that presents a large amount of cottonseed hulls, the data from this study are partly consistent with the studies of Chizzotti et al. (2005), who observed that cottonseed hulls increased the DM intake without changing the weight gain. Moore, Poorem, and Swingle (1990) found that animals that consumed cottonseed hulls instead of alfalfa hay had greater homogeneity of the diet in the rumen and ruminal escape probably was greater and therefore DM intake was stimulated. However, the greater DM intake of animals consuming CSB did not avoid the use of this by-product in the diets with greater EE content. CBS is considered a cheap ingredient [on April, 2009, the price of the ingredient was US$ 372.64 and US$ 150.52 (considering US$ 1.00 = R$ 0.4589) respectively for each ton of DM of soybean meal and CSB]. The use of CSB reduced the cost of the diet (Table 1) and thereby reduced the cost of gain, even with greater DM intake: 1.60 US$/kg of gain with the use of SOB and 1.35 US$/kg of gain with CSB, in diets containing 5% EE. As it was found a difference in DM intake in the fat sources, the increasing levels of inclusion of CSB increased linearly (P = 0.03) [equation 4] the DM intake, 8.34 ± 0.56, 9.90 ± 0.38 and 10.34 ± 0.43 kg/day of DM for diets with 3, 4 and 5% EE, respectively, and CSB as fat source. Zinn (1989a) working with feedlot, found that a fat content of 2, 5.5 and 9%EE in the diet did not affect DM intake of Brahman crossbreed animals, which is not in agreement with this study. Felton and Kerley (2004a) first observed a positive linear effect on DM intake of young crossed steers as a function of dietary EE content in 52 days of experiment with diets ranging from 4.5 to 10% EE, using soybean as fat source. Nevertheless, the same authors, in a second 72-day experiment, found no effect of increasing the EE content in diets on DM intake of animals. Vander Pol et al. (2009) in two reported experiments with diets ranging from 3.5 to 8.8%EE also found no effect on DM intake of animals. Contrary to the previous studies, Costa et al. (2011) found a linear decrease in DM intake of cattle as levels of cottonseed were increased, and justified this reduction as an effect of the reduction in fiber digestibility and the increase in rumen retention time of ingestion caused by greater amount of EE in the diet. Interestingly, the same authors found that this effect occurred in diets with levels of EE above 5%, and, in this experiment, 5% was the maximum threshold used. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 60 An influence (P < 0.05) of fat sources and increasing levels of CSB was found for protein intake (Table 2). In 3% EE diets, the fat sources did not influence (P ≥ 0.05) the protein intake, absolute and relative to BW; however, greater protein intake (P < 0.05) was found in the 5% EE diet and CSB. This increase in protein intake is due to the greater DM intake and the diet protein content (Table 1). The non-significance (P ≥ 0.05) of the contrasts that compared the neutral detergent fiber (NDF) intake of as a function of the fat sources (C1 and C2) was contrary to what was expected, especially in 5CSB, which presented greater DM intake and it also presented a numerically greater content of NDF than the diet with SOB (Table 1). Mathematically, a greater NDF intake was expected, but did not occur due to the selectivity trait of the animal, which avoided the intake of more voluminous ingredients. Based on data from all cattle categories, Detmann et al. (2003) reported the reduction in DM intake due to the increase in the amount of NDF in the diet. The same authors also found that the peak NDF intake [2.95% BW] in tropical conditions was with 40.4% DM of NDF in the diet. A difference (P < 0.05) between extreme levels of CSB inclusion was found in the nutrient intake, with a positive linear relationship (P< 0.02) [equations 6 to 9] with CBS inclusion to protein and EE intake, due to the linear increase in DM intake [Equation 4] which increased the ingestion of these nutrients. However, NDF intake was not changed with increasing CSB levels, as the means achieved for the NDF intake proportional to BW were 1.84 ± 0.5, 1.67 ± 0.03 and 1.50 ± 0.08% for 3CSB, 4CSB and 5CSB, respectively. The overall mean NDF intake, related to BW, was 1.66 ± 0.02%; Detmann et al. (2003) reported a NDF intake of 1.12%, with a range from 0.03 to 2.21%, regarding animals of various categories and an average NDF content of 48.4% DM in the diets. Ítavo et al. (2002) found an average NDF intake of 0.99% BW for Nellore cattle at age and weight similar to those used in this experiment, using diets with 18% DM of protein. Compared to the previous studies, the increase in the relative NDF intake here reported is due to the use of CBS, which, despite being included in the diet as fat source, it is also an ingredient rich in fiber and protein. 3.2 Feeding Behaviour Regarding the values related to the ingestive behaviour of the animals (Table 3), no influence (P ≥ 0.05) of the fat source and CSB inclusion at feeding time was observed, but an effect of increasing inclusion (P < 0.001) of CSB in the rumination time of the animals in feedlot was found. The DM intake in the behaviour observation days did not follow the pattern of over the experiment, whereas, on the behaviour observation days, a greater DM intake was found with the use of CSB (P < 0.05) in the diets with 5% EE, like the all experiment; however, there was no difference (P ≥ 0.05) of DM intake with the inclusion of CSB, as it occurred with the analysis of data of the entire experiment (Table 2). Fat sources (SOB and CSB, at 3 and 5 % of EE in the diet) and the CSB increasing levels did not change (P ≥ 0.05) the feeding time, absolute and relative to the DM intake. A greater feeding was expected with the greater DM intake in diets with 5% EE and CSB as fat source; however, such difference was not observed, demonstrating good acceptance of CSB by animals. The rumination time was also not affected by the CSB (P ≥ 0.05) compared to SOB at 3 and 5% of EE in the diet; however, the CSB addition in the diet increased linearly (P < 0.001) [equation 10 and 11] the rumination time. Considering that an increase in the physically effective NDF in the diet increases the time spent in rumination in dairy cows, as observed by Beauchemin and Yang (2005), together with the information that the CSB increase did not change the NDF intake (Table 3), the physical effectiveness of the NDF of this by-product was demonstrated by the positive relationship between rumination time and content of physically effective NDF (Beauchemin & Yang, 2005). www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 61 Table 3. Feeding behaviour of Nellore cattle fed with agro-industrial by-products Component Means Treatments+ SEM++ P+++ 3SOB 5SOB 3CSB 4CSB 5CSB Model C1 C2 C3 C4 DM intake (kg/day) 8.27 7.84 7.49 8.12 8.80 8.89 0.30 0.02 0.73 0.04 0.34 0.66 Feeding time (min/day) 164 156 155 180 164 171 7.60 0.00 0.17 0.32 0.59 0.41 Feeding time/DM intake (min/kg) 20.8 21.1 20.3 25.1 19.0 19.5 1.53 0.06 0.19 0.79 0.07 0.18 Rumination time (min/day) 298 267 287 225 338 370 14.0 0.00 0.21 0.12 0.00(10) 0.17 Rumination time /DM intake (min/kg) 37.1 33.9 38.9 31.1 36.6 42.7 2.06 0.02 0.46 0.26 0.00(11) 0.91 Lying time (min/day) 897 893 929 881 897 884 10.7 0.08 0.68 0.13 0.92 0.57 +3SOB and 5SOB = diets with 3 and 5% ether extract and soybean by-product as lipid source; 3CSB, 4CSB and 5CSB = diets with 3, 4 and 5% ether extract and cottonseed by-product as lipid source, respectively. ++SEM = standard error of the mean, consider n=40. +++ C1 = 3SOB vs 3CSB; C2 = 5SOB vs 5CSB; C3 = linear effect of CBS inclusion; C4 = quadratic effect of CBS inclusion, contrasts tested by Scheffé’s test. DM = dry matter. Rumination Time = 21.11 + 72.5 x EE of the diet with CSB (R2=0.47; P<0.001) (10) Rumination Time/DM intake = 13.77 + 5.79 x EE of the diet with CSB (R2=0.30; P<0.001) (11) Another explanation for the increase in rumination time may be due to the greater intake of EE by animals, which probably caused a decrease in apparent digestibility of fiber, which is one of the factors responsible for stimulating rumination, as noted by Zinn (1989b). However, provided that it does not reduce animal performance (Table 2) and does not impair carcass traits (Table 4), this apparent reduction of fiber digestibility can be seen as an advantage in using more concentrated diets. Beauchemin and Yang (2005) reported that increase the rumination time, due to physical effectiveness of the fibre and subsequent buffering of rumen pH, therefore avoiding clinical and subclinical acidosis and allowing the inclusion of a greater proportion of concentrated ingredients, with a proportional reduction of roughage ingredients. 3.3 Carcass Evaluation The fat sources used, CSB and SOB at 3 and 5% of EE in the diet, did not affect (P ≥ 0.05) the carcass traits of the animals (Table 4), which is in agreement with Zinn (1989a), Felton and Kerley (2004b), Cranston et al. (2006), Larraín, Schaefer, Arp, Claus, and Reed (2009),Vander Pol et al. (2009), who also found no changes in carcass traits using different fat sources in diets for feedlot cattle. The extreme levels of CSB changed (P < 0.05) KPH, in kg, but the data did not fit a linear regression equation (P = 0.08) [Equation 12] and the effect became non-significant (P ≥ 0.05) when the data were corrected for hot carcass weight (HCW). The amount of forequarter was found to be greater (P = 0.02) in the treatment with 5CSB than in the 3CSB (C3) treatment, with a positive linear relationship (P = 0.01) [Equation 13] as CSB was increased in the diet, but no differences were found when the forequarter was expressed in proportion to the cold carcass weight. Thus, the increasing inclusion of CSB in the diet did not affect carcass traits, which is similar to the observations of Felton and Kerley (2004a), who found no changes in the carcass of animals fed increasing levels of soybean. The overall mean of KPH in relation to HCW in this study was greater than the one found by Cranston et al. (2006) (2.33 versus 4.68 ± 0.13%); however, the animals in this study showed a lower mean of HCW than the animals studied by Cranston et al. (2006) (362.2 versus 250.4 ± 4.74 kg). The average value of KPH was also greater than that observed by Bonilha et al. (2008), in summarized data of eight progenies of Nellore animals of the same pedigree of those used in this study; they found KPH of 7.62 kg, and HCW of 277.5 kg, with a percentage ratio of 2.74%. In addition, Larraín et al. (2009) found a mean of KPH of 3.64% in Angus with average HCW of 354 kg. www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 62 Table 4. Estimated means of carcass traits of Nellore cattle fed with agro-industrial by-products Component Means Treatments+ SEM++ P+++ 3SOB 5SOB 3CSB 4CSB 5CSB Model C1 C2 C3 C4 HCW (kg) 250 245 257 239 254 256 4.74 0.23 0.49 0.84 0.09 0.36 CCW (kg) 247 243 254 236 251 253 4.69 0.24 0.44 0.87 0.09 0.38 HCW/ Final BW (%) 55.4 55.7 55.8 55.2 55.6 54.7 0.22 0.54 0.45 0.13 0.55 0.30 Carcass length (cm) 125 126 125 124 124 125 0.88 0.52 0.56 0.99 0.67 0.54 CCW/Carcass length (kg/cm) 1.98 1.93 2.02 1.89 2.02 2.01 0.03 0.85 0.53 0.82 0.10 0.21 Liver (kg) 5.09 5.04 5.19 4.76 5.29 5.19 0.09 0.51 0.36 0.99 0.19 0.23 Liver/HCW (%) 2.05 2.07 2.02 2.00 2.09 2.04 0.04 0.91 0.36 0.79 0.59 0.13 KPH (kg) 11.8 11.1 12.3 9.8 12.4 12.6 0.40 0.01 0.26 0.79 0.03(12) 0.03 KPH/HCW (%) 4.68 4.58 4.75 4.06 4.87 4.90 0.13 0.24 0.19 0.70 0.06 0.07 Forequarter (kg) 90.3 88.3 93.2 83.2 93.2 93.5 2.00 0.00 0.09 0.90 0.02(13) 0.07 Forequarter/CCW (%) 37.0 37.1 37.1 35.8 37.7 37.5 0.27 0.11 0.11 0.64 0.06 0.13 Spare Ribs (kg) 35.0 34.2 35.6 34.0 36.2 35.3 0.75 0.86 0.93 0.89 0.52 0.36 Spare Ribs /CCW (%) 14.4 14.4 14.6 14.1 14.2 14.7 0.14 0.55 0.51 0.97 0.34 0.60 Hindquarter (kg) 118 116 121 115 118 120 2.15 0.56 0.84 0.80 0.27 0.90 Hindquarter/CCW (g kg-1) 48.6 48.6 48.7 49.6 47.7 48.3 0.24 0.18 0.19 0.65 0.14 0.06 LM area (cm2) 74.7 74.7 78.6 74.0 77.3 68.8 1.24 0.14 0.86 0.12 0.22 0.09 LM area/HCW (cm/100 kg) 30.1 30.5 31.0 31.1 30.7 27.3 0.63 0.24 0.73 0.09 0.08 0.31 SBF (mm) 3.62 3.27 3.45 3.46 4.87 3.05 0.27 0.23 0.82 0.64 0.66 0.14 SBF/HCW (mm/100 kg) 1.46 1.35 1.37 1.45 1.90 1.22 0.10 0.25 0.77 0.66 0.53 0.15 Cutability (%) 51.8 52.1 52.2 52.3 51.5 51.1 0.17 0.39 0.61 0.34 0.65 0.63 Cutability (kg) 128 127 132 124 129 129 2.30 0.25 0.49 0.43 0.26 0.44 3SOB and 5SOB = diets with 3 and 5% ether extract and soybean by-product as lipid source; 3CSB, 4CSB and 5CSB = diets with 3, 4 and 5% ether extract and cottonseed by-product as lipid source, respectively. ++SEM = standard error of the mean, n=40. +++C1 = 3SOB vs 3CSB; C2 = 5SOB vs 5CSB; C3 = linear effect of CBS inclusion; C4 = quadratic effect of CBS inclusion, contrasts tested by Scheffé’s test. HCW = hot carcass weight; CCW = cold carcass weight; BW = body weight; KPH = kidney, pelvic and heart fat; LM area = M. longissumus area; SBF= subcutaneous fat thickness. KPH, kg = 1.3 + 0.02 x Initial BW + 0.7 x EE of the diet with CSB (R2=0.20; P=0.08) (12) Forequarter, kg = -7.76 + 0.26 x Initial BW + 3.59 x EE of the diet with CSB (R2=0.72; P=0.01) (13) According to the recommendations of Luchiari Filho (2000) to achieve a good yield of the cuts, the value of the LM area should be 29 cm/100 kg of HCW and fat cover should be within the range from 2 to 2.5 mm/100 kg of HCW. The values observed in this study are above the recommended ones for LM area (30.1 ± 0.63 cm/100 kg of HCW) and quite below for the subcutaneous fat (1.4 ± 0.1 mm/100 kg of HCW). In the description of the normal curve of growth of cattle made by Owens, Gill, Secrist, and Coleman (1995) the muscle growth, strong at puberty, tends to slow down with age and is followed by fat deposition (first KPH, then subcutaneous and, finally, marbling). Thus, the substantial percentage of KPH in relation to cold carcass weight observed in this study is related to the fact that these animals were slaughtered after the phase of fast muscle growth, stimulated mainly by the high levels of protein in the diets (Table 1) and after the deposition of KPH, but before the main period for subcutaneous fat deposition. Nevertheless, on average, 3.62 ± 0.27 mm of subcutaneous fat thickness was found. In addition, in all treatments, the values of the subcutaneous fat thickness fat were above www.ccsenet.org/jas Journal of Agricultural Science Vol. 6, No. 6; 2014 63 the minimal threshold of 3 mm required by the Brazilian meat industry, in contrast with the observations made by Duarte et al. (2011), who found no averages greater than 3 mm in cattle raised in pasture with more than 8 incisors teeth (about 48 months of age), this finding further demonstrates the potential use of feedlot to improve carcass fat finishing. In general, the average HCW was 250.4 ± 4.74 kg and the length was 125 ± 0.88 cm, parameters that were close to the 234.3 kg and 130 cm reported by Maggioni, Marques and Rotta (2010) for non-castrated Nellore animals. In addition, the values of 55.4 ± 0.22% for HCW/Final BW, 37.0 ± 0.27% for forequarter/HCW, 14.4 ± 0.14% for spare ribs/HCW and 48.6 ± 0.24% for hindquarter/HCW found in this work were close to those reported by Leme et al. (2000) for non-castrated Nellore cattle, that found in same traits: 53.7, 40.6, 12.6 and 46.8%, respectively; and by Zorzi et al. 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