Scientific Notes 517 FIRST RECORD OF A NATIVE HETEROPTERAN PREYING ON THE INTRODUCED EUCALYPTUS PEST, THAUMASTOCORIS PEREGRINUS (HEMIPTERA: THAUMASTOCORIDAE), IN BRAZIL Gabriely K. Souza1, TiaGo G. PiKarT1, FiliPe C. PiKarT2, JoSé eduardo Serrão3, CarloS F. WilCKen3 and JoSé C. zanunCio1 1Departamento de Entomologia, Universidade Federal de Viçosa, 36570-000 Viçosa, Minas Gerais State, Brazil 2Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, 88520-000 Lages, Santa Catarina State, Brazil 3Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viçosa, nas Minas Gerais State, Brazil 4Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, 18603-970 Botucatu, São Paulo State, Brazil The bronze bug, Thaumastocoris peregrinus Carpintero & Dellape, 2006 (Hemiptera: Thau- mastocoridae), is a threat to commercial plan- tations of Eucalyptus (Myrtales: Myrtaceae) in Argentina, Australia, Brazil, Chile, Kenya, South Africa, Uruguay and Zimbabwe (Carpintero & Dellape 2006; Martínez & Bianchi 2010; Nadel et al. 2010; Noack et al. 2011). Thaumastocoris peregrinus is a typically gregarious sucking insect with nymphs and adults of different generations overlapping throughout the year (Noack & Rose 2007; Wilcken et al. 2010). Adults are about 3 mm long, with a flattened light brown body (Noack et al. 2011). Females lay about 60 black eggs usually in clusters on leaves, branches and at the base of host plant trunks (Jacobs & Neser 2005; Noack & Rose 2007). The high reproductive capacity of T. peregrinus facilitates rapid colonization and infestation. The pest initially causes silvering of the leaves, which subsequently shift to brown and red coloration, and this makes the trees look bronzed (Jacobs & Neser 2005). These canopy coloration changes al- low identification of the presence of T. peregrinus before leaf drop and plant death occurs. The mechanism that induces plant death is not known, but the symptoms of feeding by nymphs and adults of T. peregrinus on eucalyptus leaves are similar to those of Cardiaspina psyllids (He- miptera: Psyllidae) (Woodburn & Lewis 1973; Crawford & Wilkens 1996). The psyllids insert their stylets through the apertures of stomata to reach food supply causing cell degeneration of the mesophyll tissue that resembles the process of leaf senescence (Crawford & Wilkens 1996). The use of the systemic insecticide imidaclo- prid injected into the trunk has been shown effec- tive to control T. peregrinus in field tests (Noack et al. 2009), but chemical control in forest planta- tions is questionable due to potentially adverse environmental impacts and high costs (Zanuncio et al. 1994). Thus, biological control, such as the introduction or enhancement of populations of predators, parasitoids and pathogens, is impor- tant (Silva 2000). Pest control with natural en- emies is growing in popularity; and heteropteran predators are important agents of biological con- trol in integrated pest management programs (Molina-Rugama et al. 1997; Lemos et al. 2003). Here, we report the occurrence of the preda- tory stinkbug, Supputius cincticeps Stal, 1860 (Hemiptera: Pentatomidae), nymphs feeding on T. peregrinus nymphs and adults in Lages, Santa Catarina State, Brazil. Nymphs and eggs of S. cincticeps, and nymphs and adults of T. peregri- nus were found on Eucalyptus viminalis Labill (Myrtales: Myrtaceae) trees at the Centro de Ciências Agroveterinárias of the Universidade do Estado de Santa Catarina (CAV-UDESC) (Fig. 1) in the urban perimeter of Lages, Santa Cata- rina State, Brazil (27°47’S, 50°18’W) in January and February 2011. There were high densities of eggs, nymphs and adults of T. peregrinus on the eucalypts. The infested trees exhibited silver colored leaves (Fig. 1), but defoliation was not high. Damage by Gonipterus scutellatus Gyllen- hal, 1833 (Coleoptera: Curculionidae) weevils to E. viminalis leaves was also observed (Fig. 2), in- dicating the coexistence of the bronze bug with other eucalyptus pests, causing multifaceted and potentially more severe damage. Adults of T. peregrinus were collected, stored in 70% alcohol and observed under stereomicro- scope to confirm species identification (Carpin- tero & Dellape 2006). Second and fourth instars S. cincticeps were collected and kept individually in Petri dishes (diam 9.0 × 1.2 cm) with nymphs of T. peregrinus ad libitum during 4 d for feeding behavior observation. Petri dishes were observed during five minutes at intervals of 60 min from 9 am to 6 Pm, and the number of T. peregrinus nymphs predated was counted. Subsequently the S. cincticeps nymphs were brought to the Labo- ratory of Biological Control of Insects (LCBI) of the Universidade Federal de Viçosa (UFV) where they were fed on pupae of Tenebrio molitor L. 518 Florida Entomologist 95(2) June 2012 (Coleoptera: Tenebrionidae) to obtain adults for taxonomic identification. Some of the preda- tor nymphs were maintained with T. peregrinus nymphs ad libitum as their sole food source to as certain if they could complete the life cycle feeding on this prey. The average consumption of T. pereg- rinus nymphs per S. cincticeps nymph was 10.26 ± 1.58 per day. Third instar larvae of the predator Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) showed a similar capacity, consum- ing 10.43 ± 0.51 T. peregrinus nymphs per day in laboratory (Barbosa et al. 2010). Predatory stink- bug, such as Podisus spp. and S. cincticeps, usu- ally attack/kill more prey at higher prey densities than are needed to supply their food require- ments (De Clercq & Degheele 1994, Vivan et al. 2002). This behavior tends to enable the predator to find the prey more easily (Holling 1966); and the gregariousness of T. peregrinus may facilitate the foraging behavior of S. cincticeps. Moreover, we observed that S. cincticeps reached the adult stage when it fed only on T. peregrinus nymphs, which suggests a potential novel host shift that could reduce populations of this prey. Thaumastocoris peregrinus was first reported as a pest in 2002 on eucalypts in urban areas of Sydney, Australia (Noack & Rose 2007). In subse- quent years, this pest spread to other countries, including Brazil, where it has been detected in Rio Grande do Sul, São Paulo, Minas Gerais, Es- pirito Santo, Rio de Janeiro, Mato Grosso do Sul and Paraná States (Wilcken et al. 2010), and in the western region of Santa Catarina (Savaris et al. 2011). Lages is located in the center of a major forest hub, and is a connection point between the Federal Highway BR-116, that connects Santa Catarina State to neighboring states Rio Grande do Sul and Paraná, with the Federal Highway BR-282 that crosses this state. This connection of federal highways supports the hypothesis that the transport of timber and eucalypt seedlings along these highways is a major mode of spread of T. peregrinus (Wilcken et al. 2010). Furthermore, it illustrates the vulnerability of South America to exotic pests, and the need to invest in plant health checks and quarantine, especially at ports and airports (Wilcken et al. 2010). Thaumastocoris peregrinus was recently in- troduced into Brazil, and there are no effective strategies for its control in commercial planta- tions. The wasp, Cleruchoides noackae Lin & Hu- ber, 2007 (Hymenoptera: Mymaridae), has been observed parasitizing eggs of the bronze bug in Australia (Lin et al. 2007) and the systemic in- secticide imidacloprid was tested on trees in ur- ban areas to control this pest (Noack et al. 2009 ). Supputius cincticeps is a generalist predator that naturally controls defoliating insects in Eucalyp- tus plantations in Brazil (Zanuncio et al. 1993, 1994). Moreover, this predator is easily reared in the laboratory and has potential for biological control programs (Zanuncio et al. 2004, 2005). Thus, additional studies on the biology, mass rearing and handling, and predation of S. cincti- ceps on T. peregrinus, both in the laboratory and field, should be undertaken with the goal of using this natural enemy in biological control programs of the bronze bug. Summary Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) is a threat to commercial Fig. 1. Supputius cincticeps on Eucalyptus vimina- lis leaf (shown at the center point). The leaf presents characteristic symptoms (silvering) of feeding by Thau- mastocoris peregrinus. A nymph of egg clusters of Thau- mastocoris peregrinus are shown by the white and black arrows, respectively. Fig. 2. Eucalyptus viminalis leaves showing dam- age caused by Gonipterus scutellatus (white arrows) and Thaumastocoris peregrinus (bronzed aspect of the leaves). An egg cluster of T. peregrinus is located at the tip of the black arrow. Scientific Notes 519 plantations of Eucalyptus species in South Af- rica, Argentina, Australia, Brazil, Chile, Kenya, Uruguay and Zimbabwe, and there are no ef- fective strategies for its control. We report here for the first time, the occurrence of the preda- tory stink bug Supputius cincticeps (Hemiptera: Pentatomidae) nymphs feeding on T. peregrinus nymphs and adults. Supputius cincticeps is a na- tive generalist predator that is easily reared in the laboratory and may be utilized as a biologi- cal control agent. Thus, studies on the biology and predatory capacity of S. cincticeps on T. per- egrinus should be conducted to use this natural enemy in biological control programs targeting T. peregrinus. Key Words: eucalyptus, biological control, Het- eroptera, Supputius cincticeps reSumo Thaumastocoris peregrinus (Hemiptera: Thau- mastocoridae) é uma ameaça a plantios comer- ciais de Eucalyptus na África do Sul, Argentina, Austrália, Brasil, Chile, Quênia, Uruguai e Zim- bábue e não existem estratégias efetivas para o controle desse inseto. Este trabalho registra, pela primeira vez, a ocorrência de ninfas do percevejo predador Supputius cincticeps (Hemiptera: Pen- tatomidae) predando ninfas e adultos de T. pere- grinus. Supputius cincticeps é um predador ge- neralista nativo facilmente criado em laboratório e pode ser utilizado como um agente de controle biológico. Dessa forma, estudos como a biologia e a capacidade predatória de S. cincticeps em T. peregrinus devem ser desenvolvidos visando à utilização desse inimigo natural em programas de controle biológico de T. peregrinus. Palavras-chave: eucalipto, controle biológico, Heteroptera, Supputius cincticeps aCKnoWledGmenTS We express heartfelt thanks to “Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)”, “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)” and “Fundação de Amparo à pesqui- sa do Estado de Minas Gerais (FAPEMIG)” for financial support. We thank Asia Science for English editing and correction of this manuscript. reFerenCeS CiTed barboSa, l. r., SanToS, F., barddal, H. P. o., maCHado, b. o., WilCKen, C. F., and Soliman, e. P. 2010. Preda- ção de Thaumastocoris peregrinus por Chrysoperla externa. EMBRAPA FLORESTAS, Colombo, Paraná. Comun. Técn. No. 257. CarPinTero, d. l., and dellaPé, P. m. 2006. A new spe- cies of Thaumastocoris Kirkaldy from Argentina (Heteroptera: Thaumastocoridae: Thaumastocori- nae). Zootaxa 1228: 61-68. CraWFord, S. a., and WilKenS, S. 1996. Ultrastructural aspects of damage to leaves of Eucalyptus camaldu- lensis by the psyllid Cardiaspina retator. Micron 27: 359-366. de ClerCq, P., and deGHeele, d. 1994. Laboratory mea- surement of predation by Podisus maculiventris and Podisus sagitta (Heteroptera: Pentatomidae) on beet armyworm (Lepidoptera: Noctuidae). J. Econ. Ento- mol. 87: 76-83. HollinG, C. S. 1966. The functional response of inverte- brate predators to prey density. Mem. Entomol. Soc. Canada 48: 5-86. JaCobS, d. H., and neSer, S. 2005. Thaumastocoris aus- tralicus Kirkaldy (Heteroptera: Thaumastocoridae): a new insect arrival in South Africa, damaging to Eucalyptus trees. South African J. Sci. 101: 233-236. lemoS, W. P., ramalHo, F. S., Serrão, J. e., and zanun- Cio, J. C. 2003. Effects of diet on development of Po- disus nigrispinus (Dallas) (Het., Pentatomidae), a predator of the cotton leafworm. J. Appl. Entomol. 127: 389-395. lin, n. q., Huber, J. T., and laSalle, J. 2007. The Aus- tralian genera of Mymaridae (Hymenoptera: Chalci- doidea). Zootaxa 1596: 1-111. marTínez, G., and bianCHi, m. 2010. Primer registro para Uruguay de la chinche del eucalipto, Thaumas- tocoris peregrinus Carpintero y Dellappé, 2006 (Het- eroptera: Thaumastocoridae). Agrociencia 14: 15-18. molina-ruGama, a. J., zanunCio, J. C., TorreS, J. b., and zanunCio, T. V. 1997. Longevidad y fecundidad de Podisus nigrispinus (Heteroptera: Pentatomidae) al- imentado con Musca domestica (Diptera: Muscidae) y frijol. Rev. Biol. Trop. 45: 1125-1130. nadel, r. l., SliPPerS, b., SCHoleS, m. C., laWSon, S. a., noaCK, a. e., WilCKen, C. F., bouVeT, J. P., and WinG- Field, m. J. 2010. DNA bar-coding reveals source and patterns of Thaumastocoris peregrinus invasions in South Africa and South America. Biol. Invas. 12: 1067-1077. noaCK, a. e., and roSe, H. a. 2007. Life-history of Thau- mastocoris peregrinus and Thaumastocoris sp. in the laboratory with some observations on behaviour. Gen. Appl. Entomol. 36: 27-33. noaCK, a. e., KaaPro, J., barTimoTe-auFFliCK, K., manS- Field, S., and roSe, H. a. 2009. Efficacy of Imidaclo- prid in the control of Thaumastocoris peregrinus on Eucalyptus scoparia in Sydney, Australia. Arboric. Urban For. 35: 192-196. noaCK, a. e., CaSSiS, G., and roSe, H. a. 2011. Systemat- ic revision of Thaumastocoris Kirkaldy (Hemiptera: Heteroptera: Thaumastocoridae). Zootaxa 3121: 1-60. SaVariS, m., lamPerT, S., Pereira, P. r. V. S., and Sal- Vadori, J. r. 2011. Primeiro registro de Thaumasto- coris peregrinus para o estado de Santa Catarina, e novas áreas de ocorrência para o Rio Grande do Sul, Brasil. Cienc. Rural 41: 1874-1876. SilVa, C. a. d. 2000. Microorganismos entomopatogêni- cos associados a insetos e ácaros do algodoeiro. EM- BRAPA-CNPA, Campina Grande, Paraíba. Doc. No. 77. ViVan, l. m., TorreS, J. b., VeiGa, a. F. S. l., and za- nunCio, J. C. 2002. Comportamento de predação e conversão alimentar de Podisus nigrispinus sobre a traça-do-tomateiro. Pesq. Agropec. Bras. 37: 581- 587. WilCKen, C. F., Soliman, e. P., noGueira de Sá, l. a., barboSa, l. r., diaS, T. K. r., Ferreira FilHo, P. J., and oliVeira, r. J. r. 2010. Bronze bug Thaumasto- coris peregrinus Carpinteiro & Dellapé (Hemiptera: 520 Florida Entomologist 95(2) June 2012 Thaumastocoridae) on Eucalyptus in Brazil and its distribution. J. Plant Prot. Res. 50: 184-188. Woodburn, T. l., and leWiS, e. e. 1973. A comparative histological study of the effects of feeding by nymphs of four psyllid species on the leaves of eucalypts. J. Aust. Entomol. Soc. 12: 134-138. zanunCio, J. C., naSCimenTo, e. C., GarCia, J. F., and za- nunCio, T. V. 1994. Major lepidopterous defoliators of eucalyptus, in the Southeast Brazil. Forest Ecol. Manag. 65: 53-63. zanunCio, J. C., laCerda, m. C., zanunCio Jr., J. S., za- nunCio, T. V., SilVa, a. m. C., and eSPindula, m. C. 2004. Fertility table and rate of population growth of the predator Supputius cincticeps (Heteroptera: Pentatomidae) on one plant of Eucalyptus cloeziana in the field. Ann. Appl. Biol. 144: 357-361. zanunCio, J. C., beSerra, e. b., molina-ruGama, a. J., zanunCio, T. V., Pinon, T. b. m., and maFFia, V. P. 2005. Reproduction and longevity of Supputius cinc- ticeps (Het.: Pentatomidae) fed with larvae of Zopho- bas confusa, Tenebrio molitor (Col.: Tenebrionidae) or Musca domestica (Dip.: Muscidae). Braz. Arch. Biol. Techn. 48: 771-777. zanunCio, T. V., zanunCio, J. C., naSCimenTo, e. C., and Vilela, e. F. 1993. Descrição das ninfas do predador Supputius cincticeps Stål (Heteroptera: Pentatomi- dae). An. Soc. Entomol. Bras. 22: 221-229.