S p M a C b C a A R R A A K L d D S C d 1 ( w r g l W & d t d � s s b m T a P s 0 d Carbohydrate Polymers 88 (2012) 1440– 1444 Contents lists available at SciVerse ScienceDirect Carbohydrate Polymers j ourna l ho me pag e: www.elsev ier .com/ locate /carbpol tructural characterization of a new dextran with a low degree of branching roduced by Leuconostoc mesenteroides FT045B dextransucrase ary Helen Palmuti Braga Vettori a, Sandra Mara Martins Franchettib, Jonas Contieroa,∗ Unesp–Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Laboratory of Industrial Microbiology, 24A Ave., Bela Vista, IBRC, Rio laro, SP, CEP 13506-900, Brazil Unesp–Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Laboratory of Polymer Biotreatments, 24A Ave., Bela Vista, IBRC, Rio laro, SP, CEP 13506-900, Brazil r t i c l e i n f o rticle history: eceived 11 October 2011 eceived in revised form 14 February 2012 ccepted 18 February 2012 vailable online 25 February 2012 a b s t r a c t This paper offers the physical and chemical characterization of a new dextran produced by Leu- conostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and 1H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endo- dextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the eywords: euconostoc mesenteroides FT045B extransucrase extran tructural characterization omparison with commercial B-512F FT045B dextran were determined by the measurement of the reducing value using the copper bicinchon- inate method and the measurement of total carbohydrate using the phenol–sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of d-glucose residues, containing 97.9% �-(1,6) linkages in the main chains and 2.1% �-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% �-(1,6) linkages in the main chains and 5% �-(1,3) branch linkages. extran . Introduction Dextran is an extracellular bacterial homopolysaccharide Sidebotham, 1974; Monsan et al., 2001), the main chain of hich is composed of contiguous �-(1,6)-linked d-glucopyranose esidues and branches, stemming mainly side-chains of �-(1,6) lucose units attached by �-(1,3) branch linkages to �-(1,6)- inked chains (Buchholz & Monsan, 2001; Dols, Remaud-Simeon, illemot, Vignon, & Monsan, 1998; Naessens, Cerdobbel, Soetaert, Vandamme, 2005; Robyt, 1995; Seymour & Knapp, 1980). Some extrans have �-(1,2) or �-(1,4) branch linkages, depending on he bacterial source. The exact structure of each type of dextran epends on the degree of branching, involving �-(1,2), �-(1,3) and -(1,4) linkages (Seymour & Knapp, 1980), which depends on the pecific microbial strain and, hence, on the specificity of dextran- ucrase(s) EC.2.4.1.5 involved (Jeanes et al., 1954). Dextrans can e produced by growing various bacterial strains of Leuconostoc esenteroides and Streptococcus species on a medium containing Abbreviations: DPn, number average degree of polymerization; FTIR, Fourier ransform Infrared; 1H NMR, 1H Nuclear Magnetic Resonance; MWn, number- verage molecular weight; NCBI, National Center for Biotechnology Information; yr/Ac, Pyridine/Acetate; TFA, trifluoroacetic acid; TLC, thin layer chromatography. ∗ Corresponding author. Tel.: +55 19 3526 4180; fax: +55 19 3526 4176. E-mail addresses: marypalmuti@bol.com.br (M.H.P.B. Vettori), amaramf@rc.unesp.br (S.M.M. Franchetti), jconti@rc.unesp.br (J. Contiero). 144-8617/$ – see front matter © 2012 Elsevier Ltd. All rights reserved. oi:10.1016/j.carbpol.2012.02.048 © 2012 Elsevier Ltd. All rights reserved. sucrose (Kim, Robyt, Lee, Lee, & Kim, 2003). Dextran production is influenced by a number of factors: physical and chemical properties (Jeanes, 1965), such as solubility, viscosity, specific optical rotation and the content of nitrogen, phosphorus and ash in the medium, which further depends on the specific microorganisms involved (Jeanes et al., 1954). A single enzyme can catalyze the synthesis of several types of dextran linkages, thereby permitting the forma- tion of a branched polymer (Neely & Nott, 1962; Smith, Sahnley, & Goodman, 1994). On the other hand, certain bacterial strains have been shown to produce dextrans of different structures, which have been attributed to the excretion of different dextransucrases (Côté & Robyt, 1982; Figures & Edwards, 1981; Zahley & Smith, 1995). Thus, the structure of each dextran is a characteristic of the specific dextransucrase (Jeanes et al., 1954). The present study discusses the structure of a water-soluble dextran produced by a dextransucrase elaborated by L. mesenteroides FT045B. Fourier Transform Infrared (FTIR) spectroscopy, 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy and TLC (Thin Layer Chromatography) of oligosaccha- rides obtained from polymer hydrolysis by endodextranase isolated from Penicillium sp were used for the determination of the struc- ture. The number average molecular weight (MWn) and degree of polymerization (DPn) were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol–sulfuric acid method. dx.doi.org/10.1016/j.carbpol.2012.02.048 http://www.sciencedirect.com/science/journal/01448617 http://www.elsevier.com/locate/carbpol mailto:marypalmuti@bol.com.br mailto:samaramf@rc.unesp.br mailto:jconti@rc.unesp.br dx.doi.org/10.1016/j.carbpol.2012.02.048 drate P 2 2 m C w J 2 ( ( ( 2 2 4 s s d ( M.H.P.B. Vettori et al. / Carbohy . Experimental .1. Organism L. mesenteroides FT045B was isolated from an alcohol and sugar ill plant and was provided by the Microbiology Industrial Process ontrol Division of Fermentec (Brazil). The nucleotide sequence as submitted to the GenBank sequence database (GenBank ID: F812153.1). .2. Enzymes a) L. mesenteroides B-512FMC dextransucrase (heretofore B- 512FMC dextransucrase) was obtained from a constitutive mutant of L. mesenteroides B-512FMC (Kim & Robyt, 1994; Zahley & Smith, 1995) which was provided by the Laboratory of Carbohydrate Chemistry and Enzymology, Iowa State Univer- sity (USA). The culture supernatant was concentrated by passing it through a polysulfone ultrafiltration hollow fiber cartridge (0.1 m × 1.0 m, H5P100-43, Amicon, Inc., Beverly, MA, USA) at a flow rate of 4 L min−1 at 21 ◦C. When the volume of the concen- trate was approximately 1 L, small molecules were removed by passing, (10 times) through 500 mL of 20 mM pyridine/acetic acid buffer, pH 5.2, containing 0.04% PVA 10 K and 1 mM CaCl2. The concentrated enzyme was then collected and the hollow fiber cartridge was washed with approximately 400 mL of the buffer which was added to the concentrate (Kitaoka & Robyt, 1998). B-512FMC dextransucrase activity was 89 IU mL−1, as determined by the 14C-sucrose assay (Vettori, Mukerjea, & Robyt, 2011), in which 1.0 IU = 1.0 �mol of �-d-glucose incor- porated into dextran per min. b) L. mesenteroides FT045B dextransucrase (hereinafter FT045B dextransucrase) was obtained from L. mesenteroides FT045B. The growth medium for L. mesenteroides FT045 B was sucrose (40 g L−1), yeast extract (20 g L−1), K2HPO4 (20 g L−1), CaCl2 (0.02 g L−1), MgSO4 (0.2 g L−1), NaCl (0.01 g L−1), FeSO4 (0.01 g L−1) and MnSO4 (0.01 g L−1). The strain was stored at −20 ◦C in a cryogenic solution until preparation of the inocu- lum for fermentation. The fermentation inoculum accounted for up to 5% (v/v) of the total volume of the medium. The microorganism was transferred from the stock culture to the same medium and was grown for 18 h at 25 ◦C and 150 rpm. The culture supernatant was obtained by centrifugation for 30 min at 13,000 × g and stored at 4 ◦C during the execu- tion of the experiments. FT045B dextransucrase activity was 2 IU mL−1, as determined by the 14C-sucrose assay described above. c) Dextranase from Penicillium sp was obtained from Sigma [lyophilized powder, 10–25 units mg−1 of solid]; one unit releases 1.0 �mole of isomaltose (measured as maltose) per min at pH 6.0 and 37 ◦C using dextran as substrate. The powder was dissolved in a buffer solution (about 4 mL of 20 mM Pyr/Ac, pH 5.5 for 1 mg of powder) to obtain 5 U mL−1. .3. Dextran reference The B-512F dextran was purchased from Sigma Chemical Co. .4. Dextran production The enzyme digest solution was prepared with the addition of 0 mL of buffer solution (20 mM Pyr/Ac, pH 5.2) to 50 mL of 400 mM ucrose and preheated to 30 ◦C for 10 min. The enzyme reaction was tarted by adding 10.0 mL of the dextransucrase (2 U mL−1) pro- uced by L. mesenteroides FT045B and incubated at 30 ◦C for 33 h 2 conversion periods). At the end of the reaction 200 mL of cold olymers 88 (2012) 1440– 1444 1441 ethanol was added to precipitate the synthesized dextran and the solution was kept at 4 ◦C for 24 h. The precipitated dextran was cen- trifuged at 13,000 × g for 30 min and dissolved in 100 mL of water. After complete solubilization, the solution was cooled in ice for 10 min. The dextran was precipitated again with the addition of 200 mL of ethanol, which was mixed and centrifuged for 30 min at 13,000 × g. Solubilization and precipitation were repeated once more to ensure that there was no remaining fructose or sucrose. The dextran was treated five to seven times with acetone (30 mL) and once with anhydrous ethanol (30 mL) and then placed into a vacuum oven at 40 ◦C for 18 h. 2.5. The number-average molecular weight (MWn) and degree of polymerization (DPn) The MWn and DPn (Jane & Robyt, 1984) of the dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrates using the phenol–sulfuric acid method (Fox & Robyt, 1991); DPn = [(total carbohydrates in �g of d-glucose)/(reducing value in �g of maltose)] × 1.9; MWn = [(DPn) × 162] + 18. 2.6. Dextran hydrolysis by endodextranase The dextran (10 mg) was dissolved in 960 �L of buffer solution (20 mM Pyr/Ac, pH 5.5); an aliquot of 40 �L of Penicillium sp endo- dextranase (5 U mL−1) was added and incubated at 37 ◦C for 14 h. Aliquots of 100 �L were taken at 10, 20, 30, 40, 50, 60 and 840 min. Five �L of concentrated trifluoroacetic acid (TFA) were added and the aliquots (final concentration of 6.14 M TFA) were heated to 50 ◦C for 10 min to stop the reaction. 2.7. Thin layer chromatography An aliquot of 1 �L of each sample and of the isomaltodextrin and maltodextrin standards was applied 15 mm from the bottom of a thin layer chromatography (TLC) plate (Whatman 5K) using a 5 �L Hamilton microsyringe pipette. Each spot size was kept between 2 and 3 mm distant from each other by adding 1 �L at a time, with intervals for drying. The plate was irrigated with three ascents to the top at 20–22 ◦C using, 85:20:50:70 (v/v/v/v) ace- tonitrile/ethyl acetate/1-propanol/water. After development, the carbohydrates were visualized by dipping the TLC plate into an ethanol solution containing 0.5% (w/v) �-naphthol and 5% (v/v) H2SO4. After air drying, the TLC plate was placed into an oven at 120 ◦C for 10 min. The densities of the carbohydrate spots on the TLC plate were determined using an imaging densitometer (Bio- Rad densitometer, Model GS-670) (Robyt & Mukerjea, 1994; Robyt, 2000). 2.8. 1H NMR spectroscopy One milligram of dextran from B-512F and 1 mg of dextran from FT045B were dissolved separately in 0.5 mL of pure D2O and then placed into 5 mm NMR tubes. 1H NMR spectra were obtained from a Bruker DRX 500 spectrometer, operating at 500 MHz at 25 ◦C. Ace- tone (2.22 ppm) was used as the standard for adjusting the 1H NMR chemical shifts. 2.9. Fourier-Transform Infrared spectrometric analysis The samples of FTIR spectra for the native dextran from B-512F and FT045B were prepared by mixing 150 mg of KBr with 5 mg of each dextran under press. 1442 M.H.P.B. Vettori et al. / Carbohydrate Polymers 88 (2012) 1440– 1444 Fig. 1. TLC analysis of hydrolysis of dextran FT045B with endodextranase (5 U/mL) at 37 ◦C. Lane 1, 2, 3, 4, 5, 6, and 7 refer to samples taken at 10, 20, 30, 60, 90, 120 and 840 min, respectively; IM = isomaltodextrin standards (IM = isomaltose, I G 3 a t s ( S L i p & h m i T a 8 7 d c n i Fig. 3 displays the 1H NMR spectra of the native dextran from L. mesenteroides B-512F and FT045B in D2O. The results show that the dextran from L. m. B-512F and L. m. FT045B have a simi- lar structure, although the �-(1,3) branch linkages and �-(1,6) Table 1 Assignments of individual signals in 1H NMR spectra of the native dextran from B-512F (used as standard) and from FT045B dextran. B-512F (reported values) B-512F (experimental) FT045B (experimental) 1H (ppm)a 1H (ppm) 1H (ppm) C1 H 4.99 4.91 4.90 C2 H 3.6 3.50 3.51 C3 H 3.76 3.65 3.63 C4 H 3.52 4.45 3.42 C5 H 3.88–4.04 3.85 3.85 M3 = isomaltotriose, and so forth); MS = maltodextrin standards (G2 = maltose; 3 = maltotriose, and so forth). . Results and discussion The dextran produced by L. mesenteroides FT045B had a number verage molecular weight of 91,536 Da and DPn of 565. Dex- ran hydrolysis by endodextranase requires a minimum of six or even non-substituted contiguously linked �-(1,6) glucose residues Boune, Huston, & Weigel, 1962; Côté & Robyt, 1984; Richards & treamer, 1978). Thus, the decision was made to make certain that . mesenteroides FT045B was producing a real dextran with predom- nantly �-(1,6)-glucopyranosidic linkages within the main chains rior to carrying out the other characterization methods (Buchholz Monsan, 2001; Dols et al., 1998; Seymour & Knapp, 1980). The mixture of oligosaccharides obtained from FT045B dextran ydrolysis were applied to TLC (Fig. 1) and densities were deter- ined to find the quantitative amount of each product by scanning maging densitometry, as described by Robyt and Mukerjea (1994). he limit of hydrolysis by endodextranase was expressed as the pparent conversion into isomaltose. The major products after 40 min of hydrolysis with Penicillium sp dextranase (Fig. 1, lane ) were isomaltose (33.2%) and isomaltotriose (24.6%). These were erived from the linear regions of the �-(1,6) glucan backbone hains. Moreover, the branched oligosaccharides, representing sig- ificant branches in dextran, branched isomaltotetraose (0.4%), somaltopentaose (3.9%) and higher branched isomaltodextrins Fig. 2. FTIR spectra for commercial dextran from B512F from FT045B. were obtained, indicating FT045B dextran branching. The type of the branching was then confirmed by NMR as �-(1,3) linkage. The type of linkages and the functional groups of the com- mercial dextran from L. mesenteroides NRRL B-512F and dextran from FT045B were characterized by FTIR spectroscopic analysis. Fig. 2 shows the FTIR spectra of commercial dextran from L. mesen- teroides B-512F and dextran from FT045B. Both dextrans gave very similar spectra, indicating a similar structure, although with differ- ent degrees of �-(1,3) branch linkages. Dextran from FT045B and B-512F contain �-(1,6) linkages, as demonstrated by the peak at 1010 cm−1, which characterizes the considerable chain flexibility present in dextran around this type of glycosidic bond (Shingel, 2002). The �-glycosidic bond is also confirmed by the peak at 916 cm−1 and the band at 1159 cm−1 caused by covalent vibrations of the C O C bond and glycosidic bridge. The peak at 1111 cm−1 was due to the vibration of the C O bond at the C-4 position of the glucose residue (Shingel, 2002). The hydroxyl stretching vibration of the polysaccharide caused the band in the region of 3410 cm−1 and the C H stretching vibration caused the band in the region of 2926 cm−1 (Capek et al., 2011; Liu, Lin, Ye, Xing, & Xi, 2007). The band in the region of 1639 cm−1 was due to bound water (Park, 1971). The �-(1,6) linkages were further confirmed by 1H NMR analysis. The 1H NMR spectra afford compelling evidence for the main structural features of dextran. The individual assignments derived from the present experiments and those reported in the literature are displayed in Table 1. C6 H 3.88–4.04 3.93 3.95 C6′ H 3.81–3.86 3.70 3.70 a Gil et al. (2008). M.H.P.B. Vettori et al. / Carbohydrate Polymers 88 (2012) 1440– 1444 1443 Table 2 Different linkage structures found in all dextran series and reported in literature. Percent of glycosidic linkagesa �-1,6 �-1,3 �-1,3 branch �-1,4 branch �-1,2 branch Leuconostoc mesenteroides FT 045B Sb 97.9e 2.1e B-512F S 95 5 B-742 Lc 87 13 B-742 S 50 50 B-1355 L 95 5 B-1355 S 54 35 11 B-1299 L 66 7 27 B-1299 S 65 35 B-1498 S 50 50 B-1501 S 50 50 Streptococcus downei Mfe28 12 88 Mfe28 90 10 Streptococcus mutans GS5 13 87 GS5 15 85 GS5 70 30 6715 S 64 36 6715 Id 4 94 2 a Adapted from Monsan et al. (2001). b S = soluble. c L = low soluble. d I = insoluble. e Determined in this study. Fig. 3. 1H NMR spectra of native dextran from Leuconostoc mesenteroides B512-F and FT045B in D2O. linkages differ in terms of percentage. Dextrans of different struc- tures can be produced by specific dextransucrases, each excreted by a certain bacterial strain (Zahley & Smith, 1995). Table 2 dis- plays the different linkage structures found in the dextran series produced by Leuconostoc mesenteroides, Streptococcus downei, and Streptococcus mutans as well as the comparison between FT045B dextran, with 97.9% �-1,6 linkages and 2.1% �-1,3 branch linkages, and B-512F dextran, which has about more than twice as many �-1,3 branch linkages (5%). The three signals that appear centered at ∼5.25 ppm (not shown) represent important structural characteristics of �-(1,3)- linked-d-glucosyl residues. The peak at 4.90 ppm is due to the H-1 of the �-(1,6) glucosyl residues of the main chain (Purama, Goswami, Khan, & Goyal, 2009; Seymour, Knapp, & Bishop, 1979). The integration of signals 5.25 and ∼4.90 gives the percentage of �-(1,3)-linked d-glucosyl and �-(1,6)-linked d-glucosyl linkages, respectively (Table 2). The FTIR and 1H NMR spectral analyses confirmed that the polysaccharide produced from L. mesenteroides FT045B is an �- (1,6) low-branched dextran, with 2.1% �-(1,3) branch linkages, giving an average chain length of (1/2.1) × 100 = 48 glucose units between branch linkages, with 97.9% �-(1,6) linkages. The com- mercial B-512F dextran from Sigma exhibits 5.0% branching, giving (1/5) × 100 = 20 glucose units between branch linkages, which is a significant difference. There are 21 branch linkages for every 1000 glucose units of FT045B dextran and 50 branch linkages for every 1000 glucose units of B-512F dextran, indicating a significant dif- ference in structure. B-512F dextran has 5% �-(1,3) branch linkages with an average chain length of 20 glucose units and FT045B dex- tran has 2.1% linkages with an average chain length of 48 glucose units, which is more than twice that of B-512F dextran. Branching, however, can also occur in different amounts and different distribu- tions, such as (1) random distribution on the main and/or secondary chains, (2) regular distribution on every other glucose unit in the main chains, or (3) on every glucose unit in the main chains and (4) different branch chain lengths, depending on the amount of branch- ing. Thus, there may be many different dextran structures that have yet to be identified and characterized and that may have different properties to be determined in further studies. FT045B dextran is 1 drate P o B c t B g o a s t e 4 d o a t ( 4 d g o d m A H L m S R 2 R B B C C C D 444 M.H.P.B. Vettori et al. / Carbohy ne of these, in which the branching is less than half that of the -512F commercial dextran or any other dextran that has been haracterized so far. This produces a chain length that is more than wice the length (48 vs. 20 glucose units, for FT045B dextran and -512F dextran, respectively). This increased chain length should ive different chemical and physical properties, in comparison to ther dextrans. The discovery of new dextrans that have different structures nd, hence, potentially different properties, resulting from different tructures, is important with regard to potential novel applica- ions and the potential improvement in or alterations to previously stablished applications. . Conclusions The present experiments demonstrated that the dextran pro- uced by L. mesenteroides FT045B has a structure similar to that f L. mesenteroides B-512F dextran, but with 97.9% �-1,6 linkages nd 2.1% �-1,3 branch linkages, as compared to B-512F dex- ran, which has more than twice as many �-1,3 branch linkages 5%). The FT045B dextran has an average branch chain length of 8 d-glucopyranose residues/molecule, as compared to B-512F extran, which that has an average branch chain length of 20 d- lucopyranose residues/molecule. 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Applied and Environmental Microbiology, 61, 1120–1123. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B d... 1 Introduction 2 Experimental 2.1 Organism 2.2 Enzymes 2.3 Dextran reference 2.4 Dextran production 2.5 The number-average molecular weight (MWn) and degree of polymerization (DPn) 2.6 Dextran hydrolysis by endodextranase 2.7 Thin layer chromatography 2.8 1H NMR spectroscopy 2.9 Fourier-Transform Infrared spectrometric analysis 3 Results and discussion 4 Conclusions Acknowledgements References