RSC Advances PAPER Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online View Journal | View Issue Study on the stru 2 aFaculdade de Ciências e Tecnologia, UNES Simonsen, 305, 19060-900, Presidente Pru unesp.br; Tel: +55 1832295748 bInstituto de Biociências, Letras e Ciências E Rua Cristóvão Colombo, 2265, 15054-000 S cInstituto de Qúımica de São Carlos, Univers 13566-590, Brazil † Electronic supplementary informa 10.1039/c6ra22508j Cite this: RSC Adv., 2016, 6, 104529 Received 8th September 2016 Accepted 17th October 2016 DOI: 10.1039/c6ra22508j www.rsc.org/advances This journal is © The Royal Society of C ctural and electrocatalytic properties of Ba +- and Eu3+-doped silica xerogels as sensory platforms† Paulo A. Raymundo-Pereira,abc Diego A. Ceccato,ab Airton G. B. Junior,ab Marcos F. S. Teixeira,ab Sergio A. M. Limaab and Ana. M. Pires*ab This work reports on the sol–gel synthesis of barium- and europium-doped silica xerogel and its use as an electrocatalytic sensor. Barium was chosen to assist the network framing and europium to provide electronic properties, including the Eu2+/Eu3+ redox process. The results from different molecular and structural techniques indicated that this xerogel is a composite material that combines a non-crystalline polymeric silica network with a high level of agglomeration and a low degree of reticulation formed by the hydrolysis and condensation of TEOS and M–O bonds (M ¼ Ba2+ and/or Eu3+), whereas the oxygen atoms belong to the xerogel phase. The electrochemical behavior of the silica xerogel:Ba2+,Eu3+ with different amounts of Eu3+ under several different conditions was investigated. The electrochemical sensing platform showed a well-defined redox coupling with a formal potential of 0.06 V, assigned to europium redox sites in silicate. Electrocatalytic activity was observed with an increasing anodic peak current to the isoniazid oxidation, indicating that the electrochemical sensing platform designed here was successfully achieved. 1. Introduction The development of electrocatalytic devices has been an area that has seen great advancement in recent years1–10 due to their low cost of instrumentation and the possibility of being assembled by different materials,1–12 with several reviews found in the literature.11,13–15 Furthermore, by adding different chem- ical species to the electrode surface, electron transfer in slow electrochemical reactions is facilitated9 as the physicochemical nature of the electrode/solution interface is changed. The operation mechanism of these devices depends on the proper- ties of the material immobilized at the electrode surface to promote electron transfer toward the target species.16 Carbon paste electrodes (CPEs) have been extensively applied for this purpose since their surfaces are easily modied.9 Electro- catalytic devices based on CPE have been widely applied toward the determination of trace amounts of elements, the evaluation of electrochemical processes, the preparation of biosensors, P – Univ Estadual Paulista, Rua Roberto dente, SP, Brazil. E-mail: anapires@fct. xatas, UNESP – Univ Estadual Paulista, ão José do Rio Preto, SP, Brazil idade de São Paulo-USP, São Carlos, SP, tion (ESI) available. See DOI: hemistry 2016 and the investigation of electrocatalytic mechanisms and organic species.1–10 Lanthanides are very versatile and can be used in the production of glasses, ceramics,17 alloys, and electronics, and they also nd application in agriculture and natural sciences.18 Lanthanide luminescent-based chemical and biological sensors have been highlighted due to their photophysical properties and their selective emission response to specic analytes.19–23 Despite the huge and increasing number of applications using rare earth ions, proportionally there are few studies related to the development and use of rare earth-based electrocatalysts for sensors;3,11,24–34 nonetheless, this application should be explored due to the excellent catalytic behavior that lanthanides exhibit. Silica xerogels obtained from the sol–gel method have been the focus of attention for electrochemists because of their advantageous features, such as mechanical stability, durability, and the possibility of doping them with metal cations.35 In this context, our strategy was to develop a carbon paste electrode modied with a composite material that would combine a high surface area and high porosity allied with the peculiar electronic properties of europium ions. Despite the fact that 3+ is the most stable oxidation state for europium, depending on the chemical environment, europium can be stabilized in its reduced atypical 2+ state. Therefore, due to having a similar ionic radius and the same charge, Ba2+ was chosen as the network polymeric frame in order to provide suitable sites to stabilize Eu2+ ions, thus favoring the electrochemical Eu3+/Eu2+ redox process.36 To fulll the physical characteristics of the material, the chosen RSC Adv., 2016, 6, 104529–104536 | 104529 http://crossmark.crossref.org/dialog/?doi=10.1039/c6ra22508j&domain=pdf&date_stamp=2016-11-01 https://doi.org/10.1039/c6ra22508j https://pubs.rsc.org/en/journals/journal/RA https://pubs.rsc.org/en/journals/journal/RA?issueid=RA006106 RSC Advances Paper Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online synthesis method was the sol–gel method.37–39 Finally, in order to evaluate the potential of this system for use in sensing devices, the electrocatalytic activity of the silica xerogel:Ba,Eu was explored to identify isoniazid molecules in physiological media. 2. Experimental section 2.1 Synthesis of silica xerogel:Ba2+,Eu3+ via the sol–gel route Silica xerogels containing Ba2+ and Eu3+ (xerogel:Ba2+,Eu3+) were synthesized via the sol–gel route by using Ba(CH3COO)2 (VETEC, 99.9%), Eu2O3 (Aldrich, 99.99%), C8H20O4Si, (TEOS, Fluka, 99.9%), and CH3COOH (VETEC, 97%) as the starting reagents. A sample containing only barium, i.e., silica xero- gel:Ba2+, was prepared as a control sample. The amount of Ba2+ ions was kept constant by using 2eqBa(CH3COO)2:1eqTEOS, while the amount of Eu3+ ions was varied relative to the Ba2+ ions as 0.5, 1.0, 5.0, and 10 ch% (charge percentage). Here, we considered an isoelectronic doping process or charge compen- sation mechanism (CCM), since Ba2+ and Eu3+ have different charges, whereby for each three Ba2+ ions taken, we introduced two Eu3+ ions, so the total positive charge remained unchanged. For the gel synthesis, stoichiometric amounts of barium and europium acetates dissolved in 10 mL of acetic acid and TEOS in an isopropyl alcohol solution (5.55 � 10�1 mol L�1) were used as the starting reagents (2Ba2+:1 TEOS). The doping percentage was calculated in relation to Ba2+ ions. Then, 0.4 mL of deionized water was added to the mixture to initiate the hydrolysis reactions. Acetic acid was also added and acted as a solvent and catalyst. The mixture was kept under stirring for 6 h at room temperature until gel formation. The gel was thermally treated at 100 �C in a static air atmosphere for 2 h to allow solvent evaporation, resulting in the xerogel phase. Then, the silica xerogel:Ba2+,Eu3+ (1.0 ch%) sample was calcinated at 100, 200, 300 or 400 �C in an O2 atmosphere with a heating ramp of 5 �C min�1 for 2 h in an EDG muffle furnace type. 2.2 Structural and molecular characterization The produced xerogel samples were used to develop a sensory platform, and their electrochemical performance were investi- gated by using cyclic voltammetry. The inuence of several parameters, such as pH, oxidizing atmosphere, supporting electrolyte, europium amount (%), and potential scan rate, on the electrochemical behavior of the silica xerogel:Ba2+,Eu3+ were analyzed by the cyclic voltammetry. The sample xero- gel:Ba2+,Eu3+ (1.0%) calcinated at 100 �C was also characterized by Fourier Transform Infrared Spectroscopy (FTIR) using a Shi- madzu Spectrometer IR Affinity-1 model; by scanning electron microscopy (SEM) using a Carls Zeiss model EVO LS15 scanning electron microscope with a detector of secondary electron (SE) in a high vacuum and at constant temperature; and by thermal analysis using a Universal V45A TA Instruments, no ow of air, and a heating ramp of 10 �C min�1 up to 1200 �C. Solid-state NMR of 29Si{1H} cross-polarization experiments were conduct- ed using a Bruker Avance spectrometer at a magnetic eld of 9.4. The 29Si{1H} CP-MAS spectra were measured under 104530 | RSC Adv., 2016, 6, 104529–104536 a spinning speed of 10 kHz and using a CP contact time of 2.5 ms and relaxation delay of 5 s. All the spectra were acquired with TPPM proton decoupling during the data acquisition applying decoupling pulses. Chemical shis are reported herein relative to the TMS referencing standard. 2.3 Preparation of electrocatalytic devices with silica xerogel:Ba2+,Eu3+ As described elsewhere,24,40 the modied carbon paste electrode (MCPE) used to set up the sensory platform was prepared by carefully mixing 55 wt% of graphite powder (1–2 mm particle size, Aldrich), 25 wt% silica xerogel:Ba2+,Eu3+ with different percentages of Eu3+ (0, 0.5, 1.0, 5.0, or 10 ch%), and 20 wt% of mineral oil (Aldrich), for a total mass of 0.5 g. This mixture was prepared under magnetic stirring in a Becker (50 mL) contain- ing 20 mL of hexane. The nal paste was obtained by evapora- tion of the solvent. The modied carbon paste was packed into an electrode body, consisting of a plastic cylindrical tube (o.d. 7 mm, i.d. 4 mm) equipped with a stainless steel staff serving as an external electric contact. Appropriate packing was achieved by pressing the electrode surface (surface area of 12.6 mm2) against a lter paper. 2.4 Reagents and solutions for electrochemical assays All the solutions were prepared using water puried with a Millipore Milli-Q system. All the chemicals were of analytical grade and used without further purication. The supporting electrolyte used for the initial experiments was a 0.1 mol L�1 KCl solution. The isoniazid (1.0 � 10�3 mol L�1) was prepared in solutions of NaCl 0.1 mol L�1, and then used as the storage solution. The solutions were purged with nitrogen to remove dissolved oxygen before the measurements. 2.5 Voltammetric apparatus All the voltammetric measurements were carried out in a 50 mL thermostatted glass cell at 25 �C with a three-electrode cong- uration: a sensory platform as the working electrode, a satu- rated calomel electrode (SCE) as the reference electrode, and platinum wire as the auxiliary electrode. During the measure- ments, the solution in the cell was neither stirred nor aerated. The measurements were conducted with a mAutolab type III (Eco Chimie) connected to a microcomputer and controlled by GPES soware. 3. Results and discussion 3.1 Silica xerogel doped samples characterization Thermogravimetric (TG) and differential scanning calorimetry (DSC) analyses for the silica xerogel:Ba2+,Eu3+ (1.0 ch%) and xerogel:Ba2+ samples showed a mass loss of approximately 5%, with exothermic peaks around 450 �C related to the formation of barium carbonate, and an endothermic peak at 1100 �C with no mass loss related to the formation of barium silicate (Fig. 1). X-ray diffraction patterns recorded for the xerogel:Ba2+ sample heated under 450 �C and another one heated under 1100 �C This journal is © The Royal Society of Chemistry 2016 https://doi.org/10.1039/c6ra22508j Fig. 1 Thermogravimetric (TG) and differential scanning calorimetry (DSC) analyses for the silica xerogel:Ba2+,Eu3+ (1 ch%) sample. Fig. 2 FTIR spectra of (a) silica xerogel:Ba2+ and (b) silica xero- gel:Ba2+,Eu3+ (1 ch%), both in KBr pellets. Fig. 3 SEM images of silica xerogel:Ba2+ (a) and (b); and silica xero- gel:Ba2+,Eu3+ (1 ch%) (c) and (d). Paper RSC Advances Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online supports this phase assignment, and can be found in the ESI, S1.† The solid-state 29Si NMR spectra for the silica xerogel:Ba2+ and silica xerogel:Ba2+,Eu3+ (1.0 ch%) samples treated at 100 �C, as seen in Fig. S2(a) and (b),† exhibit three peaks, each at�90.70 (�92.98) ppm, �104.38 (�103.60) ppm, and �112.35 (�111.96) ppm, assigned to the Si(OSi)2(OH)2 group (Q2), Si(OSi)3(OH) group (Q3), and Si(OSi)4 group (Q4), respectively, all corresponding to the inorganic polymeric structure of silica.41 Since the CP-MAS (29Si{1H}) cross-polarization acquisition mode was used, those groups that have hydrogen atoms near by the silicon nucleus are intensied; therefore, in both samples, the most intense signal corresponds to the Q3 site. Probably, the Si(OSi)3(OH) groups are on the xerogels surface. The intensity of the signal assigned to the Q2 groups apparently increases for the sample containing europium, but this could be associated with the europium paramagnetic effect on the silica surface causing a signal loss. In the FTIR spectra of the silica xerogel:Ba2+ and silica xerogel:Ba2+,Eu3+ (1 ch%) samples, as seen in Fig. 2, bands centered near 1014 cm�1 and 892 cm�1 assigned to the (Si–O– Si)n and Si–OH vibration modes, respectively, can be observed, which conrms the formation of polymeric silica as a result of TEOS hydrolysis and condensation. Also, the presence of vibration modes with an energy lower than 700 cm�1 related to M–O bonds (M¼ Eu3+ and/or Ba2+) ensure that metallic ions are combined to oxygen atoms in the xerogel network. Bands centered near 1550, 1420, and 1280 cm�1 indicate the presence of carbonates in the xerogel structure namely originating from the thermal decomposition of acetic acid. SEM images of the silica xerogel:Ba2+ and silica xero- gel:Ba2+,Eu3+ (1 ch%) samples treated at 100 �C, as seen in Fig. 3, show that both samples exhibit a high level of agglom- eration. According to Rios et al.,42 the presence of non- hydrolyzable groups from TEOS leads to a xerogel with a lower degree of reticulation.42 This journal is © The Royal Society of Chemistry 2016 3.2 Inuence of the silica xerogel:Ba2+,Eu3+ synthesis conditions on the sensory platform electrochemical performance To assess the effect of the europium doping concentration in the silica xerogel:Ba2+,Eu3+ on the sensory platform, all the samples were prepared by heating the gel at 100 �C for 2 h. Voltammetric proles were evaluated in 0.1 mol L�1 KCl solu- tion at pH 5.8, while the cyclic scans were conducted in the unstirred solution at a potential scan rate of 50 mV s�1 from �0.50 to +0.50 V vs. SCE, recording rst the oxidation step and then the reduction step in the presence of oxygen. All the vol- tammograms shown in Fig. 4 were recorded in similar condi- tions. Fig. 4(a) shows the inuence of the amount of Eu3+ ions varying from 0.5 ch% up to 10 ch% in the silica xero- gel:Ba2+,Eu3+ phase on the anodic and cathodic peak current signal of the sensory platform. A dened current peak can only RSC Adv., 2016, 6, 104529–104536 | 104531 https://doi.org/10.1039/c6ra22508j Fig. 4 Cyclic voltammograms obtained at a potential scan rate of 50 mV s�1 between +0.5 and�0.5 V vs. SCE in the presence of oxygen for the sensory platform with: (a) silica xerogel:Ba2+,Eu3+ containing 0.5, 1.0 5.0, or 10 ch% of Eu3+ ions; (b) xerogel:Ba2+,Eu3+ (1 ch%) pretreated thermally at 100–400 �C; and (c) silica xerogel:Ba2+, xerogel:Ba2+,Eu3+ (1 ch%), and Eu2O3. 104532 | RSC Adv., 2016, 6, 104529–104536 RSC Advances Paper Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online be observed for the sample containing 1.0 ch% of Eu3+ ions in the xerogel. When the concentration is higher than 1.0 ch%, the ionic mobility in the electrode surface is blocked, making the electron transfer process difficult in the xerogel, consequently suppressing the electrochemical process in the electrode surface. For lower concentrations, e.g., the 0.5 ch% sample, we observe that the electrochemical process does not take place; probably the amount of active species is not enough to promote the oxidation/reduction processes at the surface when the potential scan is applied onto the sensory platform. Thus, the silica xerogel:Ba2+,Eu3+ containing 1.0 ch% of Eu3+ ions was selected for the further studies because it exhibited the best voltammetric prole with a well-dened peak potential. The silica xerogel:Ba2+,Eu3+ (1 ch%) sample was thermally treated at different temperatures prior to the preparation of the sensory platform, and the results are shown in Fig. 4(b). It is possible to observe that the anodic and cathodic peak currents dramati- cally decrease for the sensory platform prepared with the silica xerogel samples that were heat-treated under higher tempera- tures, even disappearing completely for the sensory platform with the xerogel treated at 400 �C. By increasing the treatment temperature, the xerogel gradually acquires a long distance organization, creating a crystalline structure, whereas europium ions canmove to the interstices, thus decreasing its efficiency in terms of the electron transfer process and ion mobility. Thus, Eu3+ ions within a crystalline network have difficulty in changing their oxidation state. Consequently, the sensory plat- form prepared with silica xerogel:Ba2+,Eu3+ (1 ch%) treated at 100 �C was selected for further investigation. In order to verify if the electrochemical response was due to the composite combined properties or the individual components, in Fig. 4(c) we compare the voltammetric proles of the sensory platform prepared with silica xerogel:Ba2+, silica xerogel:Ba2+,Eu3+ (1 ch%), and pure europium(III) oxide (Eu2O3). Only the sample containing silica xerogel:Ba2+,Eu3+ (1 ch%) exhibits a typical cyclic voltammogram, with two peaks at +0.061 V (EPA) and �0.168 V (EPC), both remaining stable aer the second cycle, with a quasi-reversible wave with a DEP ¼ 229 mV. The peaks can be attributed to the reversible single-electron-reduction/ oxidation process of the EuII/EuIII pair. This is evidence of Eu redox activity for the electrode, although in general the redox peaks are somewhat ill dened.43,44 3.3 General electrochemical properties of the silica xerogel:Ba2+,Eu3+ sensory platform The surface concentration of electroactive species (G/mol cm�2) was estimated from the background-corrected electric charge (Q), under the anodic peaks in accordance with the theoretical relationship31 as follows G ¼ Q/nFA, where Q (C) is the background-corrected electric charge, calculated by integrating the anodic peak of the cyclic voltammogram (v ¼ 5 mV s�1) in KCl aqueous solution; n is the number of electrons transferred; F is the Faraday constant; and A is the electrode geometric area. The calculated Q value was 1.9 � 10�3 C, and the estimated surface concentration was 1.6 � 10�7 mol cm�2. To determine the inuence of the oxygen on the electrochemical behavior of This journal is © The Royal Society of Chemistry 2016 https://doi.org/10.1039/c6ra22508j Fig. 5 Evolution of the anodic peak potential of the silica xero- gel:Ba2+,Eu3+ (1 ch%) sensory platform as a function of the [ionic charge]/[ionic radii] and hydration enthalpies for alkaline ions (a) and for earth-alkaline ions (b). Paper RSC Advances Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online the sensory platform, some experiments were carried out in degassed solution, and the prole, which is included in the ESI in Fig. S3,† exhibited a marked small alteration in the redox potential in the absence of dissolved O2 (DEP(presence)�(absence) ¼ 40 mV), while the current values were not altered. Therefore all the analyses were performed in the absence of oxygen. Furthermore, this signicant difference in the current magni- tude at �0.40 V vs. SCE could be assigned to the reduction of dissolved oxygen in solution.45 Thus, these results indicate that the silica xerogel:Ba2+,Eu3+ sensory platform has promise as a sensor for dissolved oxygen. The effect of the potential scan rates (5–300 mV s�1) on the voltammetric response of the silica xerogel:Ba2+,Eu3+ sensory platform in 0.1 mol L�1 KCl solution was also investigated. The recorded cyclic voltammograms revealed that the anodic peak current increases and that the peak potential shis as the scan rate increases, as shown in Fig. S4.† The anodic and cathodic peak currents varied linearly with the square root of the scan rates (inset Fig. S4†). This linearity indicates that the redox process follows a diffusion-controlled mechanism. This behavior suggests a mobility of the counter-ions of the sup- porting electrolyte, which is necessary for charge transport or to keep the electroneutrality at the electrode surface during the redox process.46 Moreover, in Fig. S4 (ESI†), it can be seen well- dened peaks aer 300 CV, indicating that the leaching of ions is not observed. In addition, the voltammetric performance of the sensory platform was investigated in different supporting electrolytes: alkaline chlorides (CsCl, RbCl, KCl, NaCl, and LiCl), earth-alkaline chlorides (BaCl2, SrCl2, CaCl2, and MgCl2), and different sodium counter-ions (NaClO4, NaCl, NaNO3, Na2SO4, Na2CO3, and Na3PO4), all at a concentration of 0.1 mol L�1. The potential peaks (Eu(II)/Eu(III)) in different alkaline ions decrease with the decrease in the ionic radius (from Cs+ to Na+), as indicated in Fig. 5(a). The lithium ions, however, show an anomalous behavior, which can be easily explained by the high degree of hydration hindering the mobility of lithium ions. For the earth-alkaline ions, as seen in Fig. 5(b), the peak potential (Eu(II)/Eu(III)) increases in line with the decreasing ionic radius (from Ba2+ to Mg2+), demonstrating the counter-ions inuence on the voltammetric behavior of the silica xerogel:Ba2+,Eu3+ for both the alkaline and earth-alkaline ions. The peak potential was found to be linearly dependent upon the ratio [charge]/ [radius], indicating the migration of such ions into the silica xerogel:Ba2+,Eu3+ network. From these results, we assume that a mobility of the supporting electrolyte is necessary for the charge transport and electroneutrality on the electrode surface during the redox process. The inuence of the anions: chloride, acetate, nitrate, perchlorate, sulfate, phosphate, and carbonate in the support- ing electrolyte on the voltammetric behavior of the silica xero- gel:Ba2+,Eu3+ sensory platform was also investigated, and the results can be seen in Fig. S5.† When carbonate and phosphate ions were used, the redox process of Eu(II)/Eu(III) disappears because the ions are in their hydrolyzed form, and consequently they are in a lower concentration. We did not nd in this study a clear dependence of the [ionic charge]/[ionic radius] ratio with the peak potentials; however, chloride ion had the lowest peak This journal is © The Royal Society of Chemistry 2016 potentials. Therefore, the NaCl 0.1 mol L�1 solution was chosen as the best pattern due to its better voltammetric prole, and also because it had the most negative potential observed among the electrolyte solutions. The electrochemical behavior of the silica xerogel:Ba2+,Eu3+ sensory platform in a 0.10 mol L�1 NaCl solution was studied over a pH range of 2.0–12.0 using cyclic voltammetry and a potential scanning from�0.5 to +0.5 V (vs. SCE) at a 50 mV s�1 scan rate. The electrolytic solutions were adjusted with hydro- chloric acid and sodium hydroxide. The experimental results showed that the silica xerogel:Ba2+,Eu3+ sensory platform is more stable in neutral aqueous solutions than in basic solutions. The voltammetric prole of the silica xero- gel:Ba2+,Eu3+ sensory platform was slightly changed at pH 2–10, which is indicative that the mechanism did not involve a proton transfer process but rather a single electron transfer process under the experimental conditions. For pH levels higher than 10, a single anodic peak of irreversible character was observed for a more positive potential. This behavior is probably more related to the europium hydroxide formation being in equilib- rium with the silica xerogel:Ba2+,Eu3+ on the electrode surface RSC Adv., 2016, 6, 104529–104536 | 104533 https://doi.org/10.1039/c6ra22508j RSC Advances Paper Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online than to counter-ion inuences on the voltammetric perfor- mance of the silica xerogel:Ba2+,Eu3+. The peak potential and peak current values were related to the pH values but no linear relationship was obtained (Fig. S6†); thus, the pH of the elec- trolyte solution (i.e., pH 6.0) was selected for the sequencing studies. 3.4 Application of the silica xerogel:Ba2+,Eu3+ sensory platform for the electrocatalytic oxidation of isoniazid Voltammetric measurements were carried out in 0.1 mol L�1 NaCl solution (pH 6.0) containing different isoniazid concen- trations in order to obtain the analytical curve. Fig. 6 illustrates the anodic peak current for different isoniazid concentrations, suggesting an interaction between the silica xerogel: Ba2+,Eu3+ and isoniazid. The current values (at +0.06 V) obtained provided a linear relationship with the isoniazid concentration ranging from 2.5� 10�6 to 2.4� 10�5 mol L�1. From 2.4� 10�5 mol L�1 onwards, a deviation from linearity occurred owing to satura- tion of the electrode surface.47 The increase in the anodic peak current clearly shows the catalytic process of isoniazid oxidation by the central metallic cation in the silica xerogel:Ba2+,Eu3+. The suggested interaction is similar to an electrochemical–chemical (EC0) mechanism.48 Two redox steps can describe the electrocatalytic mechanism of the sensory platform. In the rst: when a positive potential higher than 0.061 V vs. SCE is applied to the working electrode, an electrochemical oxidation of silica xerogel:Ba2+,Eu2+ occurs, producing silica xerogel:Ba2+,Eu3+ on the electrode surface. When the cathodic potential is swept, the silica xero- gel:Ba2+,Eu2+ is electrochemically regenerated. In the second step, the xerogel in the oxidized form reacts with isoniazidred generating isoniazidoxi (see Fig. 6), consequently reducing the silica xerogel:Ba2+,Eu3+ to silica xerogel:Ba2+,Eu2+, which is electrochemically reduced. The increase in the magnitude of the anodic peak current obtained at 0.061 V (versus SCE) is proportional to the analyte concentration in solution. Fig. 6 Linear scan voltammograms obtained at 50mV s�1 for the silica xerogel:Ba2+,Eu3+ (1 ch%) sensory platform containing different concentrations of isoniazid. In the inset, analytical curves for isoniazid obtained from the voltammograms. 104534 | RSC Adv., 2016, 6, 104529–104536 The detection limit was calculated following the method described in the experimental section and the value obtained was 2.91 � 10�7 mol L�1. This low detection limit can be explained by the synergetic effect between the silica xero- gel:Ba2+,Eu3+ and isoniazid, which facilitates the electron transfer. The silica xerogel:Ba2+,Eu3+ sensory platform exhibited convincing repeatability without a signicant loss of electro- catalytic activity, with a relative standard deviation (RSD) lower than 5%. The results showed that the relative standard devia- tion (RSD) of the peak current of Eu2+/Eu3+ varies, where the intra-day repeatability was 3.3% (with n ¼ 10), suggesting that the prepared silica xerogel:Ba2+,Eu3+ sensory platform has good stability. This could be ascribed to the excellent stability and renewability of the surface. When ve parallel-fabricated sensors were compared for inter-day repeatability, a relative standard deviation (RSD) of 2.9% was observed, indicating a good reproducibility of the sensor assembling. Moreover, the stability of the sensor was also evaluated. Fiy measurements were recorded and the initial electrochemical response decreased by only 4.9%; these experimental results indicate that the current response is reproducible and that the silica xero- gel:Ba2+,Eu3+ sensory platform possesses long-term stability. 4. Conclusions A Ba2+,Eu3+-doped silica xerogel was fully characterized by TG, DSC, FTIR, SEM, and solid-state 29Si NMR, which showed that it is a composite material that combines a non-crystalline poly- meric silica network with a high level of agglomeration and a low degree of reticulation formed by the hydrolysis and condensation of TEOS and M–O bonds (M ¼ Ba2+ and/or Eu3+), whereas the oxygen atoms belong to the xerogel phase. Furthermore, cyclic voltammetry data indicated that Eu3+ ions present in the xerogel network facilitate the oxidation of isoni- azid at lower potentials (0.061 V vs. SCE). Therefore, the elec- trochemical properties of the Ba2+,Eu3+-doped silica xerogel in addition to its catalytic ability showed greater efficiency in the isoniazid detection, with the added advantage that the device preparation was not time consuming. Finally, the electro- chemical properties of the silica xerogel:Ba2+,Eu3+ electro- chemical sensing platform combined with its high catalytic activity yielded values for the detection limit in the micromolar range, exhibited high reproducibility and repeatability, and excellent sensitivity. In essence, this study highlights the potential improvement involved in the unexploited direct application of an inorganic composite in electrode surface modication. The low cost of the Ba2+,Eu3+-doped silica xerogel sensory platform applied to isoniazid electrocatalytic oxidation compared to graphene, carbon nanotubes, or metallic nano- particles devices, represents a competitive advantage for this device as a suitable tool for electrochemical applications. Acknowledgements The authors are thankful to the Brazilian agencies FAPESP, CNPq (302728/2012-0) and CAPES for the nancial research support. Paulo A. Raymundo-Pereira is particularly grateful to This journal is © The Royal Society of Chemistry 2016 https://doi.org/10.1039/c6ra22508j Paper RSC Advances Pu bl is he d on 1 8 O ct ob er 2 01 6. D ow nl oa de d by U N IV E R SI D A D E F E D E R A L S A O C A R L O S on 6 /5 /2 01 9 5: 56 :1 9 PM . View Article Online the São Paulo research Foundation (FAPESP) for the award of a scholarship (Grant No. 2008/07298-7, 2012/17689-9, 2016/ 01919-6 and 2015/10394-1). 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Acta, 2012, 66, 265–270. This journal is © The Royal Society of Chemistry 2016 https://doi.org/10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j Study on the structural and electrocatalytic properties of Ba2tnqh_x002B- and Eu3tnqh_x002B-doped silica xerogels as sensory platformsElectronic supplementary information (ESI) available. See DOI: 10.1039/c6ra22508j