Autoaggregation assay performed under static conditions All cultures were homogenized at the start of experiment and then kept at static conditions for further measurements. The missing values represent an outlier that was removed or strains that were not used on specific experiments. Assay 1 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.119 1.080 1.073 20 1.106 0.873 1.062 40 1.139 0.496 0.737 60 1.097 0.345 0.417 80 1.108 0.292 0.382 100 1.059 0.295 0.336 120 1.090 0.266 0.338 Assay 2 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.052 1.004 1.016 20 1.052 - 1.076 40 1.037 0.373 0.981 60 1.016 0.354 0.884 80 0.985 0.356 0.838 100 1.014 0.340 0.819 120 0.959 0.305 0.814 Assay 3 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.079 1.057 1.011 20 1.081 0.965 1.035 40 1.080 0.617 0.781 60 1.070 0.406 0.605 80 1.036 0.361 0.565 100 1.008 0.290 0.543 120 1.014 0.295 0.540 Assay 4 Time (minutes) Optic density at 600 MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.069 1.043 1.031 20 1.067 1.033 1.059 40 1.072 0.794 1.046 60 1.060 0.624 0.974 80 1.054 0.581 0.858 100 1.020 0.505 0.817 120 0.898 0.486 0.763 Assay 5 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.075 0.989 1.042 20 1.076 0.912 1.042 40 1.089 0.464 0.857 60 1.094 0.377 0.686 80 1.064 0.413 0.608 100 1.064 0.411 0.590 120 1.038 0.406 0.582 Assay 6 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.053 1.041 1.001 20 1.093 1.008 1.075 40 1.086 0.464 1.050 60 1.093 0.278 0.826 80 1.085 0.267 0.787 100 1.071 0.280 0.764 120 1.063 0.292 0.745 Assay 7 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.033 1.011 1.078 20 1.058 1.033 0.983 40 1.050 0.759 0.653 60 1.054 0.495 0.557 80 1.051 0.420 0.483 100 1.037 0.395 0.494 120 1.014 0.377 0.502 Assay 8 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.068 1.038 1.010 20 1.081 1.057 1.099 40 1.083 1.005 1.069 60 1.081 0.702 1.034 80 1.081 0.560 0.813 100 1.073 0.498 0.775 120 1.053 0.457 0.733 Assay 9 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.081 1.046 1.118 20 1.091 1.054 1.094 40 1.105 0.921 1.080 60 1.048 0.656 1.007 80 1.079 0.525 0.786 100 1.069 0.471 0.720 120 1.062 0.446 0.705 Assay 10 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.065 1.015 - 20 1.085 1.068 - 40 1.085 0.983 - 60 1.089 0.691 - 80 1.095 0.604 - 100 1.075 0.531 - 120 1.067 0.491 - Assay 11 Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.094 1.069 - 20 1.039 0.994 - 40 1.087 0.597 - 60 1.083 0.354 - 80 1.045 0.267 - 100 1.030 0.221 - 120 1.051 0.228 - Average ± Standard deviation Time (minutes) Optic density at 600 nm MS427(pBAD) MS427(pIC) MS427(pCO4) 0 1.076 ±0.025 1.012 ±0.043 1.058 ±0.041 20 1.055 ±0.029 1.032 ±0.027 1.068 ±0.033 40 1.071 ±0.020 1.040 ±0.023 1.068 ±0.036 60 1.077 ±0.033 1.049 ±0.024 1.056 ±0.041 80 1.067 ±0.045 1.044 ±0.031 1.054 ±0.043 100 1.081 ±0.021 1.039 ±0.036 1.065 ±0.035 120 1.073 ±0.039 1.029 ±0.032 1.048 ±0.055