Cytogenetic Analysis of A-, B-chromosomes and ZZ/ZW Sex Chromosomes of Characidium gomesi (Teleostei, Characiformes, Crenuchidae) Edson Luis Maistro1,*, Célia Maria de Jesus2, Claudio Oliveira3, Orlando Moreira-Filho2 and Fausto Foresti3 1 Instituto de Farmácia e Nutrição, UNIFENAS, 37130–000, Alfenas, MG, Brazil 2 Departamento de Genética e Evolução, Universidade Federal de São Carlos, 13565–905, São Carlos, SP, Brazil 3 Departamento de Morfologia, Instituto de Biociências, UNESP, Câmpus de Botucatu, 18618–000, Botucatu, SP, Brazil Received February 4, 2004; accepted February 21, 2004 Summary Different cytogenetic techniques were used to analyze the chromosomes of Characidi- um gomesi with the main objective of comparing the base composition of ZZ/ZW sex-chromosomes, B-chromosomes and the heterochromatin of A-chromosomes. The results of digestion of chromo- somes with AluI restriction endonuclease (RE), silver and CMA3 staining, C-banding and fluores- cence in situ hybridization (FISH) with the 18S rDNA probe suggested the existence of composition- al differences between the heterochromatin of ZZ/ZW sex-chromosomes, A- and B-chromosomes, and indicated the presence of different numbers and morphology of B-chromosomes in the samples of this population. Key words Fish cytogenetics, Characidium gomesi, B-chromosomes, ZZ/ZW sex chromosome system, Heterochromatin differentiation. The genus Characidium belongs to the Crenuchidae family and comprises small sized fishes which rarely exceed a standard length of 100 mm (Buckup 1991). This group includes about 58 nominal species but cytogenetic data are available for only few species of the genus Jobertina and Characidium (Miyazawa and Galetti-Jr. 1994, Maistro et al. 1998, Centofante et al. 2001). Kary- otypic data for the genus Characidium have shown a relatively stable pattern, with all species hav- ing a diploid number of 2n�50 and biarmed chromosomes (meta-submetacentrics). Sex chromo- some systems, presence of B chromosomes as well as natural triploidy were verified in some of the populations and species analyzed (Miyazawa and Galetti-Jr. 1994, Maistro et al. 1998, Centofante et al. 2001). Several reports have shown the occurrence of B chromosomes among Neotropical fish species (Salvador and Moreira-Filho 1992, Vicente et al. 1996). These chromosomes vary in size from mi- crochromosomes, such as those in Prochilodus lineatus (Maistro et al. 2000), to medium-sized chromosomes, as in Rhamdia hilarii (Fenocchio and Bertollo 1990, Maistro et al. 2002), and even macrochromosomes, as in some A. scabripinnis populations (Salvador and Moreira-Filho 1992, Maistro et al. 2001). In spite of several new reports about B-chromosomes, little information is available about their structure in fish (Vicente et al. 1996, Maistro et al. 2000, 2001, 2002). In the present study several cytogenetic techniques were employed to analyze the chromo- somes of Characidium gomesi in order to compare the general structures and base compositions of A- and B-sets of chromosomes of this species. © 2004 The Japan Mendel Society Cytologia 69(2): 181–186, 2004 * Corresponding author, e-mail: edson.maistro@unifenas.br Materials and methods Thirty-one specimens (21 females and 10 males) of Characidium gomesi from the Pardo River, Botucatu, State of São Paulo, Brazil, were analyzed. The fish were identified and have been deposited in the fish collection of the Laboratory of Genetics and Fish Biology, UNESP, Botucatu, São Paulo, Brazil. Mitotic chromosomes were obtained from a cell suspension of anterior kidney and gill tissues, as described by Foresti et al. (1993). The arm ratio limits suggested by Levan et al. (1964) were employed for the classification of chromosome types. C-banding was performed by the method of Sumner (1972), and silver-staining of the nucleolus organizer regions was performed by the tech- nique of Howell and Black (1980). Chromomycin A3 staining was performed by the method of Schweizer (1980). Restriction endonuclease (RE) AluI suspended in the appropriate buffer was ap- plied at the concentration of 0.3 U/m l to air-dried chromosome preparations, and slides were incu- bated in a moist chamber at 37°C for 4 h, washed in distilled water and stained with 5% Giemsa for 5–10 min. As a control, cells on other slides were treated with AluI buffer without enzyme. The 18S rDNA probe was produced by the polymerase chain reaction (PCR) using genomic DNA from Prochilodus affinis and the procedure for fluorescent in situ hybridization (FISH) was carried out as described by Wasko and Galetti-Jr. (2000). Results and discussion The specimens of Characidium gomesi analyzed in the present study had 2n�50 chromo- somes (32M�18SM) confirming the results obtained by Maistro et al. (1998) in which the species was identified as Characidium cf. fasciatum. A characteristic of this species is the presence of biarmed chromosomes, usually meta-submetacentrics (Miyazawa and Galetti-Jr. 1994, Maistro et al. 1998, Centofante et al. 2001). The only exception was observed for Characidium pterostictum which showed a medium-sized subtelocentric pair in its complement (Miyazawa and Galetti-Jr. 1994). Some fishes presented 1 to 4 ST/A B-chromosomes (Figs. 1, 2). The interindividual numeri- cal chromosome variation observed in C. gomesi from the Pardo river is probably due the occur- rence of meiotic instability of the supernumerary chromosomes, as was proposed for Prochilodus lineatus (Pauls and Bertollo 1990, Maistro et al. 2000) and Rhamdia hilarii (Maistro et al. 2002) populations. The karyotype of Characidium gomesi showed a small amount of heterochromatin after C- banding, with a few chromosomes showing some more clearly visible bands (Fig. 1b). C-banding patterns were basically characterized by the presence of small heterochromatic blocks in the cen- tromeric region of most chromosomes. B chromosomes were heterochromatic. This pattern is also a general feature observed in the chromosomes of other Characidium samples (Miyazawa and Galet- ti-Jr. 1994, Centofante et al. 2001). A conspicuous block was observed in the interstitial position near the centromere in the two chromosomes of pair 19 in males; in females, one of the chromo- somes of pair 19 was totally heterochromatic (Fig. 1b). This heteromorphic chromosome pair ob- served in females indicate the occurrence of a ZZ/ZW system of sex chromosome, confirming the results initially obtained by Maistro et al. (1998). A similar kind of sex chromosome mechanism was detected in Characidium gomesi from the Paiol Grande stream, where the W chromosome is also completely heterochromatic but, differently from what was observed in the present study, the W is smaller than the Z chromosome (Centofante et al. 2001). One NOR-bearing chromosome pair was detected in the most of the samples investigated by silver staining. NORs were located terminally on the long arm of pair 17 and showed positive C- banding. In one specimen, few cells showed a third chromosome with the Ag-NOR in the terminal position (Fig. 2a). Comparable Ag-NOR positions were described for a C. gomesi population from 182 Edson Luis Maistro et al. Cytologia 69(2) the Paiol Grande stream (Centofante et al. 2001). These authors also could distinguish the kary- otypes of C. gomesi and C. cf. gomesi by the NOR position. Though each population/species of Characidium shows more frequently one NOR-bearing chromosome pair, some specimens from some populations have shown multiple Ag-NORs (Miyazawa and Galetti-Jr. 1994, Maistro et al. 1998). Miyazawa and Galetti-Jr. (1994) suggest that the multiplicity of NORs observed in some Characidium gomesi populations could be a particular chromosomal characteristic of differentia- 2004 Cytogenetic Analysis of Chromosomes and ZZ/ZW Sex Chromosomes 183 Fig. 1. Karyotypes of Characidium gomesi from the Pardo River. a) Giemsa staining (in the inset, the B-chromosome), b) C-banding (in the inset, two B-chromosomes), c) After AluI restriction enzyme cleavage (in the inset, the B-chromosome); Bar�10 mm. tion, or may be due to processes of regulation of genetic activity of ribosomal cistrons, since only active cistrons in the preceding interphase are stained by silver (Miller et al. 1978). The Ag-NORs were positively stained by the CMA3 fluorochrome (Fig. 2b), suggesting that the rDNA loci of C. cf. gomesi may contain spacer sequences or NOR-associated heterochromatin rich in GC base pairs, as found in other fish species (Amemiya and Gold 1986, Maistro et al. 2002, among others). CMA3 staining also revealed that the heterochromatin located at the terminal posi- tion on the long arm of the micro B-chromosomes was rich in GC base pairs. Similar results were obtained for the Astyanax scabripinnis species from the Cascatinha stream (Maistro et al. 2001). Since some animals and plants have NORs in the B-chromosomes (Green 1990, Beukeboom 1994) we applied FISH with an 18S rDNA probe to check if the strong staining of the extremities of the B-chromosomes of C. gomesi after CMA3 staining could represent inactive NORs in this population. FISH applied to slides from four specimens revealed only one chromosome pair as de- scribed after Ag-NOR staining and did not show the presence of rDNA sites on B-chromosomes (Fig. 2c). Maistro et al. (1998) previously observed the presence of multiple NORs in some speci- 184 Edson Luis Maistro et al. Cytologia 69(2) Fig. 2. Somatic metaphases of Characidium gomesi from the Pardo River. a) Ag-NOR bearing chro- mosomes (arrows), b) CMA3 staining, c) FISH with the 18S rDNA probe (On b and c, small arrows shows B-chromosomes and large arrows shows NOR bearing chromosomes). mens of C. gomesi (identified as C. cf. fasciatum). Since the FISH technique was applied only to few specimens and revealed only a single chromosome pair with rDNA sites, the divergence ob- served could indicate the existence of interindividual polymorphism of the NOR chromosome num- ber. Further studies are necessary to clarify this question. When fixed metaphase chromosomes were digested with AluI restriction endonuclease, the telomeric regions on A-chromosomes, the heterochromatin attached to the centromere the Z and W chromosomes and the terminal region of the long arm of the ST/A B-chromosome were not digest- ed, virtually resembling the C-banding pattern, except for a few differences (Fig. 1c). These find- ings suggest that the heterochromatin of the telomeres, Z, W and the extremity of the long arm of B-chromosomes are poor in AluI sequences. The dark staining of some chromosome regions after restriction endonuclease treatment may be related to the absence of DNA sequences recognized by the enzyme in these regions or to inaccessibility of highly compacted heterochromatin for diges- tion, or to both factors (Mezzanotte and Ferrucci 1984). The results obtained with the different techniques applied in the present study suggest the existence of some compositional differences be- tween the heterochromatin present in the A-, B- and sex chromosomes of Characidium gomesi (Table 1). Similar findings were reported for other fish species when these combined techniques were used (Maistro et al. 2000, 2001, 2002, Swarça et al. 2001, among others) and have contributed to a better characterization of the fish chromosomes. The fact that only two close populations of Characidium showed B-chromosomes (Maistro et al. 1998) and that Characidium gomesi fish represent local populations restricted to small tribu- taries of some rivers, permit us to suggest an independent origin of these chromosomes in some populations. Moreover, considering that B-chromosomes probably arose from A-chromosomes (Green 1990, Beukeboom 1994), the existence of a different chromatin composition between them may indicate that the origin of B-chromosomes in Characidium gomesi is not a recent event, with enough time having elapsed for these chromosomes to accumulate some modifications on their structure during evolution. Acknowledgements The authors are grateful to Dr. Paulo Buckup for the taxonomic identification of the species. Funds supporting this study were provided by FAPESP, FAPEMIG, CNPq, and UNIFENAS. References Amemiya, C. T. and Gold, J. R. 1986. Chromomycin A3 stains nucleolus organizer regions of fish chromosomes. Copeia 1: 226–231. Beukeboom, L. W. 1994. Bewildering Bs: an impression of the 1st B-chromosome Conference. Heredity 73: 328–336. Buckup, P. A. 1991. 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