REVISTA DE ODONTOLOGIA DA UNESP Rev Odontol UNESP. 2018 Nov-Dec; 47(6): 383-387 © 2018 - ISSN 1807-2577 ORIGINAL ARTICLE Doi: https://doi.org/10.1590/1807-2577.11018 This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Alkalizing potential and calcium release of residues from intracanal dressing containing calcium hydroxide Potencial de alcalinização e liberação de cálcio de resíduos de medicação intracanal contendo hidróxido de cálcio Mariana Pires CRESPOa, Kamila de Figueiredo PEREIRAb*, Milton Carlos KUGAb, Thiago Soares PORTOc, Luciana ARMADAa aUniversidade Estácio de Sá, Curso de Odontologia, Departamento de Endodontia, Rio de Janeiro, RJ, Brasil bUNESP – Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, Departamento de Odontologia Restauradora, Araraquara, SP, Brasil cCase Western Reserve University, School of Dental Medicine, Department of Comprehensive Care, Cleveland, Ohio, United States of America Resumo Introdução: Se a restauração temporária, que sela o canal radicular contra o meio bucal, falhar ou solubilizar, a medicação intracanal pode ser parcialmente removida, diminuindo a eficiência do hidróxido de cálcio (CH). Objetivo: Avaliar o potencial alcalinizante e a liberação de íons cálcio de resíduos de diferentes medicações intracanal, em diferentes períodos. Material e método: Incisivos humanos foram endodonticamente instrumentados e divididos aleatoriamente (n=10). Os canais radiculares foram preenchidos com: G1⎯Hidróxido de cálcio (CH); G2⎯CH com 0.4% de digluconato de clorexidina (CHC); G3⎯CH com paramonoclorofenol camforado e glicerina (HPG); G4⎯CH mantido durante todo experimento, como controle positivo (PC) e G5⎯Sem medicação intracanal, como controle negativo (NC). Os espécimes foram imersos em água destilada durante 7 dias. A medicação intracanal foi removida dos grupos experimentais com um instrumento F1 (Protaper). Os dentes foram armazenados em água destilada por 24 horas, 7, 14 e 28 dias. Os dados foram submetidos aos testes ANOVA one-way e Tukey. Resultado: Todos os grupos experimentais tiveram redução no potencial alcalinizante e na liberação dos íons cálcio, quando comparados ao controle positivo (p<0.05). Não houve diferença estatisticamente significante entre os grupos experimentais, independente do período (p>0.05). Conclusão: O potencial alcalinizante e a liberação de íons cálcio dos resíduos de diferentes medicações intracanal, em diferentes períodos, foram similares entre si e menores que o controle positivo, onde a medicação foi mantida dentro do canal radicular, encorajando a sua troca quando a restauração falhar. Descritores: Curativos biológicos; hidróxido de cálcio; clorexidina; endodontia. Abstract Introduction: If the restoration, that seal the root canal from oral environment, failed or solubilize, the intracanal dressing could be partially removed, decreasing the efficiency of calcium hydroxide (CH). Objective: To evaluate the alkalizing potential and calcium ions release of intracanal residues from different medications, in different periods. Materials and method: Human incisors were endodontically instrumented and randomly divided (n=10). The root canals were filled with: G1⎯Calcium hydroxide (CH); G2⎯CH with 0.4% chlorhexidine digluconate (CHC); G3⎯CH with camphorated paramonochlorophenol and glycerin (HPG); G4⎯CH was maintained during all experiment, as positive control group (PC) and G5⎯ No medication was performed in the negative control group (NC). The specimens were immersed in distilled water for 7 days. The intracanal dressing was removed from the experimental groups using F1 instrument (Protaper). The teeth were stored in distilled water for 24 hours, 7, 14 and 28 days. pH value and calcium ions released were evaluated and the data were submitted to ANOVA one-way and Tukey tests. Result: All experimental groups presented lower alkalizing potential and calcium ions release than PC (P<0.05). No significant difference was found among the experimental groups, regardless of the period (P>0.05). Conclusion: The alkalizing potential and calcium ions release of residues from different intracanal dressing, in different periods, were similar and lower than the positive control group, that the medication was maintained inside the root canal, encouraging the replacement of intracanal dressing when the restoration fail. Descriptors: Intracanal dressing; calcium hydroxide; chlorhexidine; endodontics. Crespo, Pereira, Kuga et al. Rev Odontol UNESP. 2018 Nov-Dec; 47(6): 383-387384 384/387 INTRODUCTION Calcium hydroxide (CH) is used as intracanal dressing, because of excellent biologic and antimicrobial effects1. To facilitate the handling of the CH, some vehicles have been proposed, providing different pH values and calcium ions release2-4. Associations of CH with other antimicrobial substances, such as chlorhexidine (CHX) or camphorated paramonochlorophenol (CMPC) have also been evaluated5,6. CHX has a wide use as endodontic solution due to antimicrobial activity7. Studies have suggested that CHX can be used in combination with CH to improve antimicrobial efficacy against resistant microorganisms to CH8-10. On the other hand, the combination of CH and CMPC was proposed to promote an antimicrobial synergistic effect against some facultative or anaerobic bacteria11. CMPC forms calcium para-chlorophenolate, which maintains a high pH and allows a controlled liberation of calcium and hydroxyl ions for a longer period4,12. The efficiency of CH as intracanal dressing, in several chemical compositions, is attributed directly to the capacity of dissociating into calcium and hydroxyl ions, which promote alkalization over the root length around 120 days3,13-15. However, this medication must be removed from the root canal for final obturation and it is a difficult procedure that routinely generates residues on the root dentin surface16-18. Some researches have reported that residues may negatively interfere on the endodontic treatment success and/or interact with endodontic sealers19. Moreover, if the temporary restoration, that seal the root canal from oral environment, failed or solubilize, the medication could be partially removed, decreasing the efficiency of CH. The clinical significance of the residues persistence is still controversial. The question whether the residues presence would present similar alkalizing potential and calcium ions release is still unsolved. Therefore, the aim of this study was to evaluate the alkalizing potential and calcium ions release of residues from different dressings containing calcium hydroxide (CH), associated to polyethylene glycol (Calen), polyethylene glycol with 0.4% chlorhexidine digluconate or CMPC with glycerin, when compared with root canal totally filled (positive control group) with CH or without any dressing (negative control group), in the periods of 24 hours, 7, 14 and 28 days. The null hypothesis was that there was no difference in the alkalinizing potential and calcium ions release, in the experimental periods. MATERIAL AND METHOD Fifty extracted human mandibular incisors with similar root canal anatomy and 16mm length were selected and stored in 0.1% thymol. The root canals were endodontically instrumented with ProTaper System until F2 instrument (Dentsply, Maillefer). The root canals were irrigated with 2.5% NaOCl (Cloro Rio, São José do Rio Preto, SP, BR). After the biomechanical preparation, the teeth were filled with 17% EDTA (Biodinâmica Ind. Com., Ibiporã, PR BR) for 3 minutes, rinsed with 5ml of 2.5% NaOCl and dried using absorbent paper points. The specimens were randomly divided (n=10) according to the intracanal dressings and filled using a lentulo spiral (Maillefer, Ballaigues, Switzerland). Radiographs were taken in buccolingual direction to confirm complete filling of the root canals: G1⎯(CH): Calcium hydroxide with polyethylene glycol (Calen, SS White, São Paulo, SP, BR) at ratio of 2.5g of calcium hydroxide, 1g of zinc oxide, 0.05g of colophony and 2ml of polyethylene glycol; G2⎯(CHC): Calcium hydroxide with polyethylene glycol (Calen; SS White) and 0.4% chlorhexidine digluconate at ratio of 1.96g paste to 0.04ml of 20% chlorexidine digluconate (Farmácia Arte e Ciência Araraquara, SP, BR), according to Silva et al.10; G3⎯(HPG): Calcium hydroxide paste with camphorated paramonochlorophenol (Biodinâmica Ind. Com. Ltda, Ibiporã, PR, BR) and glycerin (SS White, São Paulo, SP, BR) at ratio of 1g: 0.5ml: 0.5ml, according to Vianna et al.3; G4⎯(PC): CH was maintained during all experiment, as positive control group; G5⎯(NC): Without intracanal dressing inside the root canal, as negative control group. The sealing of the roots was made with glass ionomer cement (Maxion R, FGM, Joinville, SC, BR), and an external waterproofing was applied as a temporary restoration on the cervical (Hidronorth, Londrina, PR, BR). Each specimen was immersed in 10ml of distilled water for 7 days. After this period, the temporary restoration was removed and the intracanal dressing was partially removed using F1 instrument (ProTaper System) in the total tooth length, except for the positive control group. Each tooth was immersed in 1ml of distilled water and the specimens were placed inside new flasks with 10ml of distilled water after 24 hours, 7, 14 and 28 days. The distilled water was exchanged every analyzed period. pH measurements were performed directly in the solutions that the specimens were immersed, using the pHmeter DM 23 (Digimed, São Paulo, SP, BR) and pH electrode DME-CV1 (Digimed, São Paulo, SP, BR), both devices were calibrated at 25°C, according to the manufacturer´s instructions. Calcium ions release was obtained using a calcium-ion selective meter with calcium-ion selective electrode (Q400I, Q838-Ca2, Quimis, Diadema, SP, BR). After calibration using 2M and 4M calcium chloride solutions (molecular weight of anhydrous calcium chloride = 110.98g/mol), two reference points were established. The ion-selective electrode was immersed in each specimen and the concentration mg/L was obtained. The data of pH value and calcium ions release were submitted to ANOVA one-way and Tukey tests, at 5% significance. In order to confirm the dressing residues presence, 3 teeth were randomly selected from each group and were longitudinally sectioned in the mesial-distal direction, and the buccal face was analyzed using a stereomicroscope, with magnification of 10x (Leica Microsystems, Germany). Rev Odontol UNESP. 2018 Nov-Dec; 47(6): 383-387 Alkalizing potential and calcium release… 385 385/387 RESULT The negative control group presented acid pH (6.10) in distilled water, and the calcium ions were not detected during all periods. The positive control presented alkaline pH (8.50) and calcium-ion presence in all periods. The means of pH and calcium ions release from experimental and control groups are described in Tables 1 and 2. The experimental groups did not differ among them regardless of the period, in both pH and calcium ions release evaluation (p>0.05), however they presented lower values than positive control group (p<0.05) and higher values than negative control group (p<0.05). Stereomicroscopy images showed the presence of residues from calcium hydroxide paste in the buccal face of the root canal, regardless of paste composition. Figure 1 shows the representative images of the calcium hydroxide residues on radicular dentin surface in the experimental and positive control groups. Table 1. Mean and standard deviation of pH values of experimental and controls groups, at different periods CH CHC HPG PC NC 24 h 6.17b (0.31) 6.30b (0.28) 6.97b (1.29) 8.50a (0.25) 6.10c (0.0) 7 days 6.22b (0.24) 6.09b (0.13) 6.22b (0.13) 8.31a (0.15) 6.10c (0.0) 14 days 6.22b (0.19) 6.11b (0.22) 6.22b (0.13) 8.51a (0.10) 6.10c (0.0) 28 days 6.21b (0.14) 6.10b (0.20) 6.22b (0.16) 8.58a (0.15) 6.10c (0.0) Different letters in the same line indicate significant differences between groups (p<0.05). CH - calcium hydroxide with polyethylene glycol; CHC - calcium hydroxide with polyethylene glycol and 0.4% chlorhexidine digluconate; HPG - calcium hydroxide with camphorated paramonochlorophenol and glycerin; PC - positive control and NC - negative control. Table 2. Mean and standard deviation of calcium ion release (mg/l) of experimental and controls groups, in different periods CH CHC HPG PC NC 24 h 2.79b (1.22) 2.67b (1.71) 3.42b (1.07) 9.51a (0.02) 0.00c (0.0) 7 days 0.38b (0.83) 0.78b (1.11) 0.92b (0.97) 7.37a (0.15) 0.00c (0.0) 14 days 1.39b (0.98) 1.86b (1.29) 1.48b (1.39) 5.31a (0.25) 0.00c (0.0) 28 days 1.34b (1.14) 1.82b (1.16) 1.51b (1.17) 4.52a (0.12) 0.00c (0.0) Different letters in the same line indicate significant differences between groups (p<0.05). CH - calcium hydroxide with polyethylene glycol; CHC - calcium hydroxide with polyethylene glycol and 0.4% chlorhexidine digluconate; HPG - calcium hydroxide with camphorated paramonochlorophenol and glycerin; PC - positive control and NC - negative control. Figure 1. Representative stereomicroscopy images of experimental groups (10X): (A) G1 - calcium hydroxide with polyethylene glycol (CH); (B) G2- calcium hydroxide with polyethylene glycol and 0.4% chlorhexidine digluconate (CHC); (C) G3 - calcium hydroxide with camphorated paramonochlorophenol and glycerin (HPG) and (D) G4 - positive control (PC). White arrows show calcium hydroxide residues on radicular dentin. Scale: 250µm. Crespo, Pereira, Kuga et al. Rev Odontol UNESP. 2018 Nov-Dec; 47(6): 383-387386 386/387 DISCUSSION The null hypothesis was rejected, because the alkalinizing potential and calcium ion release of CH paste residues with polyethylene glycol (CH), CH paste with polyethylene glycol associated with 0.4% chlorhexidine digluconate (CHC) or CH paste with CMPC and glycerin (HPG) were lower than positive control group (PC), in all periods. This study evaluated the residues effect on the alkalizing capacity and calcium ions release of intracanal dressings. The efficiency of CH is due to chemical dissociation into calcium and hydroxyl ions in aqueous or viscous solutions13,20. The calcium ions participate in mineralization healing process and the hydroxyl ions promotes an alkaline osteogenic environment in the surrounding tissues15,21,22. When used as intracanal dressing has an effective alkalizing potential and provide calcium ion release4,22. However, after intracanal removal, frequently occurs persistence of CH residues on radicular dentin, mainly in the apical radicular third16,18. In present study, the teeth length were standardized at 16 mm and instrumented until F2 instrument. This procedure aimed to standardize the root canal diameter, apical foramen and consequently the amount of CH dressing placed intracanal. After 7 days, the F1 instrument was purposely used to removal of CH dressing, in order to keep only residues in the root canals, which were confirmed by representative images made with stereomicroscopy. The pH methodology, used in this study, is established in literature23,24. The atomic absorption spectroscopy is used to evaluate the calcium release of calcium hydroxide pastes23, but this method only quantified the calcium total present in sample24. Due to the ionized calcium participate in biological processes we used selective electrodes to calcium ions assessment21,22. The results of the experimental groups were similar in all periods and presented lower pH values and calcium ions release than the positive control group. In relation to alkalizing potential, the intracanal residues of calcium hydroxide dressing were unable to promote alkalization of aqueous environment, possibly in function of the low disintegration CH residues, because of the root canal was maintained dry. Hosoya et al.25 observed that the alkalization potential of the mixtures with water were statistically quicker and greater than when the CH powder was used as intracanal dressing. The overfilling of the root canal occurred in all samples, consequently, in 24h period was detected calcium ions release, probably from residues present around of the radicular apex25. In another periods the calcium ions release was lower than 24h, possibly because the CH intracanal does not disintegrated and/or extruded to beyond to radicular apex. The positive control group (root canal filled with CH) presented the highest calcium ions release, in accordance with Duarte et al.23. Therefore, intracanal CH residues, regardless of the composition and/or mixture paste, do not induce the alkalization and calcium ions release. Further research focusing on the interaction of the residues with the filling of root canals are necessary in order to establish the possible interferences on the endodontic treatment prognosis. CONCLUSION The alkalizing potential and calcium ions release of residues from different intracanal dressings with CH, during different experimental periods, were similar and significantly lower than the medication when it was maintained inside the root canal, encouraging the replacement if the temporary restoration fail. REFERENCES 1. Siqueira JF Jr, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. 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