A new species of tube-dwelling anemone (Cnidaria, Anthozoa, Ceriantharia, Ceriantheopsis) from the Warm Temperate South-western Atlantic se’rgio n. stampar 1,2 , fabrizio scarabino 3,4,5 , guido pastorino 6 and andre’ c. morandini 7 1Universidade Estadual Paulista, UNESP/Assis, Departamento de Ciências Biológicas, Laboratório de Evolução e Diversidade Aquática – LEDA, Av. Dom Antônio, 2100, Assis, SP – 19806-900, Brazil, 2Instituto Laje Viva, Santos, Brazil, 3Dirección Nacional de Recursos Acuáticos, Constituyente 1497, C.P 11200, Montevideo, Uruguay, 4Museo Nacional de Historia Natural, C.C. 399, C.P 11000, Montevideo, Uruguay, 5Centro Universitario Regional Este – Cure, Sede Rocha, Universidad De La República, Ruta 9, Km 208, C.P 27000, Rocha, Uruguay, 6Museo Argentino de Ciencias Naturales ‘Bernardino Rivadavia’, Av. Angel Gallardo 470, 38 piso lab. 80, C1405DJR Ciudad Autónoma de Buenos Aires, Argentina, 7Universidade de São Paulo, Instituto de Biociências, Departamento de Zoologia, Rua do Matão, trav. 14, 101, São Paulo, SP, 05508-090, Brazil A new species of tube-dwelling anemone of the genus Ceriantheopsis (Cnidaria: Ceriantharia), Ceriantheopsis lineata sp. nov., is described and can be found in fine sand or mud in the sublittoral zone (0–200 m) from Argentina to Brazil (Warm Temperate South-western Atlantic). This new species is distinguished from its congeners by a number of features, however some characters (directive tentacles, line on tentacles and length of P2) allow a conclusive identification among the other Ceriantheopsis species. Keywords: Atlantic Ocean, taxonomy, Anthozoa Submitted 20 July 2015; accepted 4 October 2015; first published online 3 November 2015 I N T R O D U C T I O N Tube-dwelling anemones (Anthozoa: Ceriantharia) are organ- isms that live in benthic marine communities either in shallow or deep waters (Carlgren, 1912). The tubes produced by these animals vary between Ceriantharia groups and apparently are related to the habit of the species (Stampar et al., 2015). Due to a number of morphological and molecular features outlined in den Hartog (1977) and Stampar et al. (2014a), the subclass Ceriantharia is divided in two clades: Penicilaria and Spirularia. The most peculiar group within Spirularia is the family Cerianthidae Milne-Edwards & Haime, 1852, that is defined by lacking specialized nematocyst-bearing internal structures called acontioids or cnidorages (den Hartog, 1977). This family is composed of four genera known from adults and larvae (Ceriantheomorphe Carlgren, 1931, Ceriantheopsis Carlgren, 1912, Cerianthus Delle Chiaje, 1841 and Pachycerianthus Roule, 1904), and several ‘genera’ known only from larval forms (Tiffon, 1987; Daly et al., 2007). The genus Ceriantheopsis was described based on Ceriantheopsis americana (Agassiz in Verrill, 1864), a species found in large numbers in some areas of the USA Atlantic Coast (Shepard et al., 1986). There are only two other described species, C. austroafricana Molodtsova et al., 2011 and C. nikitai Molodtsova, 2001, both from the Atlantic coast of the African continent (Temperate Southern Africa Zone – cf. Spalding et al., 2007) (Molodtsova et al., 2011). Within the Warm Temperate South-western Atlantic (after Spalding et al., 2007) there is only one uncertain record of Ceriantheopsis (Cerianthus americana) by Hertwig (1882) from the Uruguayan coast. However, the identification of the specimen was only performed by examination of the exter- nal morphology, as the diagnostic characters for identification of genera and species are related to internal anatomy (see more in Molodtsova et al., 2011; Stampar et al., 2014b). For this geographic area there are also some Cerianthidae records without specific identification (see Scarabino, 2006). The aim of this work is to describe a species of the genus Ceriantheopsis based on both morphology and anatomy of specimens collected in Argentina and Brazil, emphasizing that this is the first species of this genus for this biogeographic region (Warm Temperate South-western Atlantic). M A T E R I A L S A N D M E T H O D S Specimens were collected by scuba diving from Quequén Port (two specimens), Buenos Aires province, in very thin grained and soft clay close to the northern breakwater (Escollera Norte), or obtained from dredging (Projeto Camarão – st 776) on the coast of Santa Catarina (one specimen), Brazil. Collected polyps were preserved in 4% seawater-buffered for- maldehyde solution for morphological studies. Many Corresponding author: S.N. Stampar Email: stampar@assis.unesp.br 1475 Journal of the Marine Biological Association of the United Kingdom, 2016, 96(7), 1475–1481. # Marine Biological Association of the United Kingdom, 2015 doi:10.1017/S0025315415001745 https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at mailto:stampar@assis.unesp.br https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core additional photographic records noted based on images taken during regular scuba diving operations of the Laje Viva NGO (www.lajeviva.org.br) – COTEC 41097/2007 to delimit the shallow distributional area (less than 50 m). Comparative spe- cimens from the American Museum of Natural History (AMNH) collection (Ceriantheopsis americana) and a speci- men of Ceriantheopsis austroafricana collected in Cape Town (South Africa) were also examined. The holotype and one paratype were deposited in the cnidarian collection of Museu de Zoologia da Universidade de São Paulo (MZUSP). Morphological study The anatomical study of the polyps and cnidome was based on criteria defined by several authors (van Beneden, 1897; Carlgren, 1912; den Hartog, 1977; Tiffon, 1987; Stampar et al., 2012). Two whole animals were cut apart through the ventral side (opposite to the siphonoglyph) using surgical scal- pels, then the dissected polyp was fastened using acupuncture needles. The classification of cnidae followed den Hartog (1977) and England (1991), but nomenclature was based solely on Molodtsova et al. (2011). Thirty measurements (undischarged capsules) were taken from each cnida type out of each body region of the holotype and paratype specimens. The cnidome was analysed under a Nikon Eclipse 80i microscope with phase contrast. All parts of the body were analysed sep- arately so that contamination would be avoided. The two parts of mesenterial filaments (cnidoglandular tract and cili- ated tract) were analysed together using 30 measurements from each part. Cnidae that showed no overlap in sizes were tested via Mann–Whitney test in order to check the con- sistency of divergence between species. Four specimens of Ceriantheopsis americana (AMNH 730/AMNH 3451) from Jamaica and USA (Florida) and one specimen of Ceriantheopsis austroafricana from Cape Town, South Africa were also examined for comparison. R E S U L T S A N D D I S C U S S I O N systematics Class ANTHOZOA Ehrenberg, 1834 Subclass CERIANTHARIA Perrier, 1883 (see details in Fig. 1. Ceriantheopsis lineata sp. nov.: (A) Live image of the holotype from Quequén, Argentina (MZUSP 2686), (B) Live specimen at Laje de Santos, São Paulo, Brazil, image only (photo from Noeli Lara Ribeiro), (C) Live specimen at Laje de Santos, São Paulo, Brazil, image only (photo from Armando de Luca Jr); (D) Live specimen from off Paranaguá, Paraná, Brazil, image only (photo from Marcelo Krause) and (E) General view of the holotype in the long tube (MZUSP 2686). 1476 se’ rgio n. stampar et al. https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at http://www.lajeviva.org.br https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core Stampar et al., 2014a) Suborder SPIRULARIA den Hartog, 1977 Family CERIANTHIDAE Milne Edwards & Haime, 1852 Genus Ceriantheopsis Carlgren, 1912 Type species – Ceriantheopsis americana (Agassiz in Verrill, 1864) by subsequent designation (Carlgren, 1912: p. 24). Distribution: The genus is restricted to the Atlantic Ocean (Caribbean Sea, Gulf of Mexico, US Coast and South Africa Coast). Diagnosis Cerianthids with alternating sterile and fertile mes- enteries. Second protomesenteries fertile, reaching aboral pole. Metamesenteries arranged in mBMb. Length of all but M-metamesenteries diminishes toward multiplication chamber. Length of M-metamesenteries of first 2–4 quartets can increase toward the multiplication chamber. M3 and further mesenteries can diminish towards multiplication chamber (Carlgren, 1912). Ceriantheopsis lineata sp. nov. Figures 1–4 type material (2 specimens) Holotype: MZUSP 2686, adult specimen (29 cm long), �12 m depth, Quequén Port (38834′41.39′′S, 58841′49.80′′W), Quequén, Buenos Aires, Argentina, G. Pastorino coll. (10/xii/2008) (I). See pictures of the animal inside the tube (Figure 1E). Paratype: MZUSP 2687, adult specimen (18 cm long), 130 m depth, offshore Joinville (26819′00′′S 47800′00′′W), Santa Catarina, Brazil, Projeto Camarão (st 776) coll. (12/ xii/1969) (II). comparative material examined AMNH 730, Ceriantheopsis americana, Jamaica; AMNH 3451, Ceriantheopsis americana, USA (Florida). First author collection, Ceriantheopsis austroafricana, from Waterfront (33854′29′′S 18825′6′′E) (10/v/2013), 8 m, Cape Town, South Africa. diagnosis Marginal tentacles with a dark longitudinal line. Two mesen- teries attached to siphonoglyph, absence of directive labial tentacle and four types of microbasic b-mastigophores. Description (based on MZUSP 2686 and 2687 specimens) Long (up to 300 mm long) and thin (10–20 mm width) cer- ianthid; 48–68 brown, purple or green marginal tentacles (40– 45 mm in preserved specimens), one dark line over tentacle length (Figure 1), arrangement 1212.1212.1212, with more than 8 pores per tentacle, unpaired labial tentacle absent; 40– 60 brown to purple labial tentacles (up to 10 mm long in pre- served specimens), arrangement 4231.4231.4231, unpaired labial tentacle absent; pleated stomodeum extending over 1/5 to 1/6 of total body length, hyposulcus 5 mm long, hemisulci distinct; siphonoglyph narrow, connected only to directives; free part of sterile directive mesenteries almost the same length of siphonoglyph, without mesenterial filament. Second protomesenteries almost reaching aboral pole, fertile, bearing ciliated tract with bundle of craspedonemes, followed by very short cnido-glandular tract and long (�70% of the mesentery) craspedion tract. Third protomesenteries sterile, longer than half directives, with craspedonemes. M and m-metamesenteries long, fertile; M1 reach 2/3 of total body length and M2 reaching almost aboral pore (longer than M1), with bundle of craspedo- nemes; B and b-mesenteries, sterile, with almost same length; see Figure 2 for schematic arrangement of mesenteries. The cnidome of the species (Figure 4) is composed of spirocysts, holotrics, microbasic b-mastigophores (four types) and ptycho- cysts distributed as shown in Table 1. Distribution – from Argentina, Quequén (Buenos Aires) to Brazil, Laje de Santos (São Paulo) (Figure 3). The southern- most record is Quequén (Buenos Aires) and the northernmost record is from Laje de Santos, São Paulo state. On the coast of Argentina (higher latitudes) specimens can be observed at shallow waters (less than 10 m) in mud. Specimens from Brazil were recorded in much deeper areas (30–130 m) and coarser sediment (fine to medium sand). Etymology – The specific name ‘lineata’ (Latin linea), female, is an allusion to the longitudinal central line on mar- ginal tentacles. Colour variation – The colour of marginal tentacles vary from yellowish-brown to a deep purple or pale green but always with a dark longitudinal line. The labial tentacles are always very similar to predominant colour of marginal tentacles. Tube symbiosis – Phoronida specimens were found on tubes of Ceriantheopsis lineata sp. nov., at Laje de Santos (Brazil), but not identified like the ones observed by Stampar et al. (2010) over Ceriantheomorphe brasiliensis. Other epizoic animals like the bryozoan colonies were not Fig. 2. Graphical representation of the arrangement of mesenteries Ceriantheopsis lineata sp. nov. MC, Multiplication chamber; dm – Directives, M – M-mesenteries, m – m-mesenteries, B – B-mesenteries, b – b-mesenteries, TP – Terminal pore and S – Siphonoglyph. a new species of ceriantheopsis ( ceriantharia ) 1477 https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core found, as described by Vieira & Stampar (2014) for Pachycerianthus schlenzae. Holotype description (MZUSP 2686) (Figures 1 & 2) Elongated polyp, 28 cm long, 25 mm diameter just below mar- ginal tentacles and 10 mm near aboral end. Marginal tentacles 67, arranged in two pseudocycles, 35–40 mm long and 6– 5 mm in diameter near the base, brown with a darkish longi- tudinal line. The space between cycles of marginal and labial tentacles brown coloured. Labial tentacles 49, about 15 mm long, brown with a white longitudinal line, directive labial absent, arrangement of marginal tentacles 1212.1212.1212. . . and labial tentacles 4231.4231.4231. . . . Oral disk 25 mm wide, stomodeum 60 mm long, light brown, siphonoglyph narrow and elongate with 2 mesenteries attached, hyposulcus 5 mm long with short hemisulci 2 mm long. Free part of dir- ective mesenteries inconspicuous, without mesenterial fila- ment. Second protomesenteries almost reach aboral pole, fertile, bearing ciliated tract with bundle of craspedonemes. Third protomesenteries sterile, longer than half directives, with craspedonemes. M and m-metamesenteries long, fertile; M1 reach 2/3 of total body length and M2 reaching almost aboral pore (longer than M1), with bundle of craspedonemes; B and b-mesenteries, sterile, with almost same length. Morphological remarks The observed specimens of Ceriantheopsis lineata sp. nov. varied much more on the colouration of tentacles than in relation to internal anatomy. The mesenteries have the same size ratio in relation to column length of the observed speci- men. The small specimen (MZUSP 2687) has fewer mesenter- ies and tentacles. The mesenterial filaments have from 0.5 to 5 mm in length and these are always arranged in the upper region of the mesentery. The amount of these structures varies greatly between mesenteries of the same type in the same specimen. The single paratype has different column length in relation to holotype; however the ratio between the length of mesenteries and column length is the same. The arrangement of the tentacles was also constant in both observed specimens. Some specimens were taken out from the tube and main- tained alive during several days in laboratory inside an artifi- cial tube (PVC) where the animal reconstructed the tube in less than 2 days. It is consistent with information about the reconstruction of the tube of Ceriantheomorphe brasiliensis (see more in Stampar et al., 2015). Comparison with other Ceriantheopsis species morphological aspects A review of the three species of the genus was recently per- formed by Molodtsova et al. (2011), and a comparison of Ceriantheopsis lineata sp. nov. is possible. With this new species, the genus Ceriantheopsis again confirmed to be restricted to Atlantic waters, two species occurring in the African coast and other two from the American coast. The first morphological trait that was possible to compare is the number of marginal tentacles. Based on this feature there are two different observed patterns; more than 100 Fig. 3. Estimated distributional area of Ceriantheopsis lineata sp. nov. along the Warm Temperate South-western Atlantic (Grey line). 1 – Quequén, Argentina (Holotype); 2 – off Joinville (Paratype) and 3 – Laje de Santos, Brazil (photographic records). 1478 se’ rgio n. stampar et al. https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core (C. americana) and fewer than 70 (other three species) tenta- cles. A second character that can be used to distinguish species is the presence of directive labial tentacle. This feature is observed in all species of Ceriantheopsis, except C. lineata sp. nov. (Table 2). Another interesting characteristic is the number of mesenteries attached to the siphonoglyph region. Ceriantheopsis lineata sp. nov. has only 2 mesenteries while all other species have 4 mesenteries attached to the Fig. 4. Cnidome of Ceriantheopsis lineata sp. nov. (A) ptychocysts I, scale bar 40 mm; (B) ptychocysts II, scale bar 20 mm; (C) holotrichs, scale bar 20 mm; (D) microbasic b-mastigophores I, scale bar 15 mm; (E) microbasic b-mastigophores II, scale bar 18 mm; (F) microbasic b-mastigophores III, scale bar 16 mm; (G) microbasic b-mastigophores VI, scale bar 30 mm. Table 1. Cnidome of Ceriantheopsis lineata sp. nov. based on two specimens, the holotype (MZUSP 2686) and paratype (MZUSP 2687); data based on 30 measurements of each type, values are presented as the mean and range in parentheses. Length (mm) Width (mm) Ptychocysts I 88.65 (84–93.6) 31.86 (25.2–34.8) Ptychocysts II 65.73 (61.8–69.6) 15.15 (15–15.6) COLUMN Holotrichs 37.92 (36–39.6) 7.2 (7.2) b-mastigophores I 30.6 (27.6–35.4) 4.98 (4.8–6) b-mastigophores II 35.16 (31.2–37.2) 5.94 (5.4–6) MARGINAL TENTACLES b-mastigophores I 28.98 (24–31.2) 4.8 (4.8) b-mastigophores II 35.16 (31.2–37.2) 5.94 (5.4–6) b-mastigophores VI 59 (54–65.4) 2.56 (2.4–3) LABIAL TENTACLES b-mastigophores I 34.3 (30–40.2) 6 (6) b-mastigophores II 25.68 (25.2–27) 3.54 (3–3.6) b-mastigophores III 32.16 (29.4–34.8) 4.8 (4.8) CNIDO-GLANDULAR TRACT b-mastigophores I 25.5 (24.6–27) 6 (6) b-mastigophores II 22.92 (21.6–24.6) 3.6 (3.6) b-mastigophores III 17.1 (15.6–18.6) 3.6 (3.6) ACTINOPHARYNX b-mastigophores I 41.94 (39.6–44.4) 6 (6) b-mastigophores II 31.56 (29.4–33.6) 3.6 (3.6) b-mastigophores III 23.34 (22.8–24.6) 4.92 (4.8–5.4) Holotrichs 41.3 (38–43.34) 8.3 (7–8.6) a new species of ceriantheopsis ( ceriantharia ) 1479 https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core siphonoglyph (Table 2). The presence of an exclusive type of b-mastigophore (type VI) nematocyst can also be assigned as a defining character of Ceriantheopsis lineata sp. nov. (Table 2). Nomenclatural remarks As previously mentioned, the genus Ceriantheopsis was created by Carlgren, 1912 to accommodate the described species Cerianthus americanus. However, this modification was performed without the specific name being suited to the agreement of correct gender. Names ending in ‘—opsis’ are feminine (ICZN – Article 30.1.2 – Examples; International Commission on Zoological Nomenclature, 1999). Thus, two specific names (C. americanus and C. austroafricanus) of the genus need to be suitable for this gender concordance. Thus, the genus composition should be as listed below. Ceriantheopsis americana (Agassiz in Verrill, 1864) Ceriantheopsis austroafricana Molodtsova et al. 2011 Ceriantheopsis lineata sp. nov. Ceriantheopsis nikitai Molodtsova, 2001 A C K N O W L E D G E M E N T S We would like to thank Dr Estefania Rodrı́guez (American Museum of Natural History) for providing specimens. We are grateful to Dr Mark Gibbons (Western Cape University) and Michael Farquhar (Two Oceans Aquarium) for the help with African specimens. We are also indebted to Dr Aline Benetti and Dr Luiz Ricardo L. Simone (Museu de Zoologia – USP) by their assistance in the curation of materials. We are also thankful to Noeli Lara Ribeiro, Marcelo Krause and Armando de Luca Jr (Nautilus Dive Center) for remarkable images, Dr Dale Calder (Royal Ontario Museum) for the help about nomenclature concordance and two anonymous reviewers for useful comments. F I N A N C I A L S U P P O R T SNS was supported by CAPES through Programa de Pós-Graduação em Zoologia, IBUSP. This work was partly supported by São Paulo Research Foundation (FAPESP 2012/01771-8 to SNS, and 2010/50174-7 to ACM), and CNPq 481549/2102-9 to SNS, 301039/2013-5 and 476339/ 2013-8 to ACM. This is a contribution of the NP-BioMar, USP. R E F E R E N C E S Carlgren O. (1912) Ceriantharia. The Danish Ingolf-Expedition 5, 1–78. Daly M., Brugler M.R., Cartwright P., Collins A.G., Dawson M.N., Fautin D.G., France S.C., McFadden C.S., Opresko D.M., Rodriguez E., Romano S.L. and Stake J.L. (2007) The phylum Cnidaria: a review of phylogenetic patterns and diversity 300 years after Linnaeus. Zootaxa 1668, 127–182. England K.W. (1991) Nematocysts of sea anemones (Actiniaria, Ceriantharia and Corallimorpharia: Cnidaria): nomenclature. Hydrobiologia 216/217, 691–697. T ab le 2. C om pa ri so n of an at om ic al fe at ur es of th e va lid sp ec ie s of C er ia nt he op si s (m od ifi ed fr om M ol od ts ov a et al ., 20 11 ). C . am er ic an a C . ni ki ta i C . au st ro af ri ca na C . li ne at a sp .n ov . M ar gi na l te nt ac le s U p to 10 0 – 12 0 U p to 70 U p to 70 U p to 60 D ir ec ti ve la bi al te nt ac le P re se nt P re se nt P re se nt A bs en t A rr an ge m en t of la bi al te nt ac le s (2 )4 13 .4 23 2. 43 12 (4 )4 13 .4 23 1. 43 12 .4 31 2 (3 )4 23 .4 23 2. 43 12 .4 31 2 (2 )3 13 .4 34 3. 43 24 .3 12 4 42 31 .4 23 1. 42 31 .4 23 1 A ct in op ha ry nx 1/ 12 – 1/ 8 of ga st ri c ca vi ty 1/ 5 – 1/ 4 of ga st ri c ca vi ty 1/ 10 – 1/ 8 of ga st ri c ca vi ty 1/ 6 – 1/ 5 of ga st ri c ca vi ty O ra ld is c 0. 7 – 1. 0 cm � 0. 6 – 0. 7 cm W id e, � 1. 5 cm in pr es er ve d 1. 0 – 1. 5 cm in pr es er ve d Si ph on og ly ph N ar ro w ,4 m es en te ri es at ta ch ed W id e, 4 m es en te ri es at ta ch ed W id e, 4 m es en te ri es at ta ch ed N ar ro w ,2 m es en te ri es at ta ch ed D ir ec ti ve m es en te ri es , A ct in op ha ry nx � A ct in op ha ry nx � A ct in op ha ry nx , A ct in op ha ry nx P 2 T o ab or al po le T o ab or al po le T o ab or al po le A lm os t to ab or al po le P 3 ¼ B ¼ B ¼ B ¼ B M . B , 2B . B ≥ B M 3 ≤ M 2 . M 2 ≤ M 2 , H al f M 2 C ni do -g la nd ul ar tr ac t at fe rt ile m es en te ri es of fir st qu ar te ts P re se nt N ot pr es en t P re se nt P re se nt C ra sp ed io n tr ac t at fe rt ile m es en te ri es 6/ 7 – 8/ 9 3/ 5 6/ 7 � 6/ 7 – 8/ 9 C ni do -g la nd ul ar tr ac t at B ≪ b ¼ b , b , b C ra sp ed on em es of cr as pe di on at fe rt ile m es en te ri es So m et im es pr es en t A bs en t A bs en t A bs en t 1480 se’ rgio n. stampar et al. https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core den Hartog J.C. (1977) Descriptions of two new Ceriantharia from Caribbean Region, Pachycerianthus curacaoensis n. sp. and Arachnanthus nocturnus n. sp., with a discussion of the cnidom and of the classification of the Ceriantharia. Zoologische Mededelingen 51, 211–248. Hertwig R. (1882) Report on the Actiniaria dredged by H.M.S. Challenger during the years 1873–1876. Report on the Scientific Results of the Voyage of the H.M.S. Challenger during the years 1873–76 (Zoology) 6, 1–136. International Commission on Zoological Nomenclature (1999) International code of zoological nomenclature, 4th edn. London: The International Trust for Zoological Nomenclature. Molodtsova T. (2001) Cerianthids (Anthozoa, Cnidaria) from Bengual upwelling region. 1. Ceriantheopsis nikitai. Zoologicheskii Zhurnal 80, 773–780. [In Russian] Molodtsova T.N., Griffiths C.L. and Acuna F.H. (2011) A new species of shallow-water cerianthid (Cnidaria: Anthozoa) from South Africa, with remarks on the genus Ceriantheopsis. African Natural History 7, 1–8. Scarabino F. (2006) Faunı́stica y taxonomı́a de invertebrados bentónicos marinos y estuarinos de la costa uruguaya. In Menafra R., Rodrı́guez-Gallego L., Scarabino F. and Conde D. (eds) Bases para la conservación y el manejo y de la costa uruguaya. Montevideo: VIDA SILVESTRE (Sociedad Uruguaya para la Conservación de la Naturaleza), pp. 113–142. Shepard A.N., Theroux R.B., Cooper R.A. and Uzmann J.R. (1986) Ecology of Ceriantharia (Coelenterata, Anthozoa) of the Northwest Atlantic from Cape Hatteras to Nova Scotia. Fishery Bulletin 84, 625–646. Spalding M.D., Fox H.E., Allen G.R., Davidson N., Ferdaña Z.A., Finlayson M., Halpern B.S., Martin K.D., McManus E., Molnar J., Recchia C.A. and Robertson J. (2007) Marine ecoregions of the world: a bioregionalization of coast and shelf areas. Bioscience 57, 573–583. Stampar S.N., Beneti J.S., Acuña F.H. and Morandini A.C. (2015) Ultrastructure and tube formation in Ceriantharia (Cnidaria, Anthozoa). Zoologischer Anzeiger 254, 67–71. doi: 10.1016/ j.jcz.2014.11.004 Stampar S.N., Emig C., Morandini A.C., Kodja G., Pinto A.P.B. and Silveira F.L. (2010) Is there any risk in a symbiotic species associating with an endangered one? A case of a phoronid worm growing on a Ceriantheomorphe tube. Cahiers de Biologie Marine 51, 207–211. Stampar S.N., Maronna M.M., Kitahara M.V., Reimer J.D. and Morandini A.C. (2014a) Fast-evolving mitochondrial DNA in Ceriantharia: a reflection of Hexacorallia paraphyly? PLoS ONE 9, e86612. doi: 10.1371/journal.pone.0086612. Stampar S.N., Maronna M.M., Vermeij M.J., Silveira F.L. and Morandini A.C. (2012) Evolutionary diversification of banded tube-dwelling anemones (Cnidaria; Ceriantharia; Isarachnanthus) in the Atlantic Ocean. PLoS ONE 7, e41091. doi: 10.1371/ journal.pone.0041091. Stampar S.N., Morandini A.C. and Silveira F.L. (2014b) A new species of Pachycerianthus (Cnidaria, Anthozoa, Ceriantharia) from Tropical Southwestern Atlantic. Zootaxa 3827, 343–354. doi: 10.11646/ zootaxa.3827.3.4 Tiffon Y. (1987) Ordre des Cérianthaires. In Grassé P. (ed.) Traité de Zoologie: Anatomie, Systematique, Biologie – Cnidaires/Anthozoaires, Tome III(3). Paris: Masson, pp. 210–256. van Beneden E. (1897) Les Anthozoaires de la ‘Plankton-Expedition’. Ergebnisse der Plankton-Expedition der Humbolt-Stiftung 2, 1–222. Verrill A.E. (1864) List of the polyps and corals sent by the Museum of Comparative Zoölogy to other institutions in exchange, with annota- tions. Bulletin of the Museum of Comparative Zoology 1, 29–60. and Vieira L.M. and Stampar S.N. (2014) A new Fenestrulina (Bryozoa, Cheilostomata) commensal with tube-dwelling anemones (Cnidaria, Ceriantharia) in the tropical southwestern Atlantic. Zootaxa 3780, 365–374. doi: 10.11646/zootaxa.3780.2.8. Correspondence should be addressed to: S.N. Stampar Universidade Estadual Paulista, UNESP/Assis, Departamento de Ciências Biológicas, Laboratório de Evolução e Diversidade Aquática – LEDA, Av. Dom Antônio, 2100, Assis, SP – 19806-900, Brazil email: stampar@assis.unesp.br a new species of ceriantheopsis ( ceriantharia ) 1481 https://www.cambridge.org/core/terms. https://doi.org/10.1017/S0025315415001745 Downloaded from https://www.cambridge.org/core. UNESP-Universidade Estadual Paulista, on 28 Jun 2019 at 19:33:45, subject to the Cambridge Core terms of use, available at mailto:stampar@assis.unesp.br https://www.cambridge.org/core/terms https://doi.org/10.1017/S0025315415001745 https://www.cambridge.org/core A new species of tube-dwelling anemone (Cnidaria, Anthozoa, Ceriantharia, Ceriantheopsis) from the Warm Temperate South-western Atlantic INTRODUCTION MATERIALS AND METHODS Morphological study RESULTS AND DISCUSSION Outline placeholder SYSTEMATICS DIAGNOSIS Holotype description (MZUSP 2686) (Figures 1 &’; 2) Morphological remarks Comparison with other &emphasis type= Outline placeholder Outline placeholder MORPHOLOGICAL ASPECTS Nomenclatural remarks ACKNOWLEDGEMENTS FINANCIAL SUPPORT REFERENCES