Virulence ISSN: (Print) (Online) Journal homepage: www.tandfonline.com/journals/kvir20 Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans Paulo Henrique Fonseca Do Carmo, Anna Carolina Pinheiro Lage, Maíra Terra Garcia, Newton Soares da Silva, Daniel Assis Santos, Eleftherios Mylonakis & Juliana Campos Junqueira To cite this article: Paulo Henrique Fonseca Do Carmo, Anna Carolina Pinheiro Lage, Maíra Terra Garcia, Newton Soares da Silva, Daniel Assis Santos, Eleftherios Mylonakis & Juliana Campos Junqueira (2024) Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans, Virulence, 15:1, 2416550, DOI: 10.1080/21505594.2024.2416550 To link to this article: https://doi.org/10.1080/21505594.2024.2416550 © 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Published online: 19 Oct 2024. Submit your article to this journal View related articles View Crossmark data Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=kvir20 https://www.tandfonline.com/journals/kvir20?src=pdf https://www.tandfonline.com/action/showCitFormats?doi=10.1080/21505594.2024.2416550 https://doi.org/10.1080/21505594.2024.2416550 https://www.tandfonline.com/action/authorSubmission?journalCode=kvir20&show=instructions&src=pdf https://www.tandfonline.com/action/authorSubmission?journalCode=kvir20&show=instructions&src=pdf https://www.tandfonline.com/doi/mlt/10.1080/21505594.2024.2416550?src=pdf https://www.tandfonline.com/doi/mlt/10.1080/21505594.2024.2416550?src=pdf http://crossmark.crossref.org/dialog/?doi=10.1080/21505594.2024.2416550&domain=pdf&date_stamp=19%20Oct%202024 http://crossmark.crossref.org/dialog/?doi=10.1080/21505594.2024.2416550&domain=pdf&date_stamp=19%20Oct%202024 https://www.tandfonline.com/action/journalInformation?journalCode=kvir20 RESEARCH ARTICLE Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans Paulo Henrique Fonseca Do Carmo a*, Anna Carolina Pinheiro Lage b*, Maíra Terra Garcia a, Newton Soares da Silva a, Daniel Assis Santos c, Eleftherios Mylonakis d, and Juliana Campos Junqueira a aDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, SP, Brazil; bDepartment of Medicine, René Rachou Research Centre, Fiocruz Minas, Belo Horizonte, MG, Brazil; cDepartment of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; dDepartment of Medicine, Houston Methodist Hospital, Houston, TX, USA ABSTRACT Candida albicans is an opportunistic yeast capable of causing a wide range of mucosal, cutaneous, and systemic infections. However, therapeutic strategies are limited to a few anti- fungal agents. Inorganic nanoparticles have been investigated as carrier systems for antifungals as potential new treatments. In this study, we focused on the antifungal activity of gold nanorods, a specific rod-shaped gold nanoparticle, produced by green synthesis using resvera- trol as a metal-reducing agent. The synthesis method resulted in stable control nanoparticles (AuNp) and resveratrol-coated gold nanoparticles (AuNpRSV) with medium sizes of 32.4 × 15.9 nm for AuNp, and 33.5 × 15.3 nm for AuNpRSV. Both AuNp and AuNpRSV inhibited the C. albicans grown at 2.46 µg/mL, exhibited fungicidal effects at 4.92 µg/mL, and significantly decreased filamentation, biofilm viability, reactive oxygen species production and ergosterol levels of C. albicans. In addition, exposure to AuNpRSV reduced the ability of C. albicans to grow in the presence of cell membrane stressors. Transmission electron microscopy revealed enlarge- ment of the cell wall and retraction of the cell membrane after treatment with AuNp and AuNpRSV. Promisingly, in vivo toxicity analysis demonstrated that both nanoparticles maintained the full viability of Galleria mellonella larvae at 49.20 µg/mL. In conclusion, both gold nanopar- ticles exhibited antifungal activity; however, these effects were enhanced by AuNpRSV. Altogether, AuNps and AuNpRSVs are potential antifungal agents for the treatment of C. albicans infections. ARTICLE HISTORY Received 20 December 2023 Revised 7 June 2024 Accepted 5 September 2024 KEYWORDS Gold nanoparticles; Biofilm; Ergosterol; Filamentation; Reactive oxygen species Introduction Candida albicans is an opportunistic yeast that colo- nizes the oral cavity, skin, gastrointestinal tract, and reproductive tract in approximately 50% of healthy individuals [1,2]. Alterations in the host immune response, changes in the resident microbiota, and other factors can lead to C. albicans overgrowth, result- ing in a wide range of infections [3]. The pathogenicity of C. albicans is associated with hyphal formation, secretion of proteolytic enzymes, haemolytic activity, and biofilm formation [4,5]. The ability to form bio- films on host tissue surfaces, medical devices, and den- tal prostheses is considered one of the most important virulence factors of C. albicans [6,7]. Biofilm structure protects C. albicans cells from antifungal therapies [8,9] and impairs treatment success [8,10]. C. albicans infections are usually treated with polyenes, azoles, and echinocandins [11]. However, these antifungal agents have limitations in terms of toxicity, recalcitrance, route of administration, high cost, and resistance [4,12,13]. To overcome these limitations, new therapeutic strategies are under investigation. One of these strategies involves studying the antifungal activity of polyphenols and the use of carrier systems [14–17]. Polyphenols are natural organic compounds with one or more phenolic groups of low molecular weight that exhibit anticancer, anti-inflammatory, antioxidant, and antimicrobial activities [18,19]. Previous studies have shown that resveratrol inhibits the planktonic and biofilm growth of C. albicans [16,20]. Among car- rier systems, gold nanoparticles have been widely stu- died because of their adjustable physicochemical properties and the potential to deliver antifungal com- pounds [21–23]. Additionally, gold nanoparticles can have a multi-target mode of action, which may reduce the occurrence of antifungal resistance [15,24]. CONTACT Anna Carolina Pinheiro Lage anna.lage@fiocruz.br *Both authors contributed equally and shared first authorship. VIRULENCE 2024, VOL. 15, NO. 1, 2416550 https://doi.org/10.1080/21505594.2024.2416550 © 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. http://orcid.org/0000-0003-3186-885X http://orcid.org/0000-0003-1663-369X http://orcid.org/0000-0002-1193-2909 http://orcid.org/0000-0001-6452-9278 http://orcid.org/0000-0002-1108-5666 http://orcid.org/0000-0002-4624-0777 http://orcid.org/0000-0001-6646-6856 http://www.tandfonline.com https://crossmark.crossref.org/dialog/?doi=10.1080/21505594.2024.2416550&domain=pdf&date_stamp=2024-10-17 Recently, green synthetic methods have emerged as innovative approaches for the production of metal nano- particles. Green synthesis employs compounds of natural origin (plant or microorganism extracts) to reduce metal ions and obtain more stable nanoparticles [25]. Green synthesis is an eco-friendly and sustainable approach that uses an environmentally favourable solvent system instead of toxic reducing agents. Some studies have inves- tigated the effects of gold nanoparticles produced by green synthesis on C. albicans. Gold nanoparticles asso- ciated with olive leaf extract inhibited the planktonic growth of C. albicans and showed enhanced activity com- pared to the antifungal nystatin [26]. In a separate study, Judan et al. [27] synthesized gold nanoparticles using extracts from 15 ethnobotanicals and observed their abil- ity to reduce biofilm formation by C. albicans [15,24]. Since resveratrol is considered a bioactive compound against C. albicans and gold nanoparticles are an effec- tive carrier system for antifungal agents, in this study, we employed resveratrol to produce green gold rod- shaped nanoparticles. Control gold nanoparticles (AuNps) and resveratrol-coated gold nanoparticles (AuNpRSVs) were synthesized and characterized. Their effects were evaluated against planktonic cells, biofilms, and virulence factors of C. albicans. Additionally, its mode of action on fungal cells and toxicity in Galleria mellonella model were investigated. Material and methods Chemicals The chemicals employed for each assay are described as follow: chloroauric acid (HAuCl4), silver nitrate (AgNO3), cetyltrimethylammonium bromide (CTAB), resveratrol (RSV), and sodium borohydride (NaBH4) for the synthesis of gold nanorods; potassium bromide (KBr) tablets for the infrared spectroscopy; fluconazole (FCZ), amphotericin B (AMB), CTAB and RSV for the susceptibility assay; sodium bicarbonate (NaHCO3) and morpholino- propanesulfonic acid (MOPS) for preparation of RPMI- 1640 medium; potassium hydroxide (KOH) and n-heptane for the ergosterol analysis; antifungals AMB, FCZ, and the reagents sodium chloride (NaCl), Congo red (RED), and calcofluor white (CFW) for stressor agents in the spot tests; paraformaldehyde, glutaraldehyde, osmium tetroxide (OsO4), potassium ferricyanide (C6N6FeK3), and calcium chloride (CaCl2) for microscopic analysis. Green synthesis of gold nanoparticles Gold nanoparticles were synthesized as described pre- viously [28]. The growth solution was prepared using 50 μL of 0.65 mm HAuCl4, 13 μL of 0.11 mm AgNO3, 50 mm CTAB, and 5 mm RSV (Sigma-Aldrich, St. Louis, MO). Then, 600 μL of 3.0 mm NaBH4 solu- tion was added, and the reaction mixture was heated to 70°C. The gold-to-silver ratio (6:1) was kept constant, and after 4 h, the reaction medium was centrifuged for 20 min at 10,000 × g. Then, the pellet was collected and resuspended in 100 mL distilled water [28]. This pro- cess was performed three times to eliminate residues. Characterization of gold nanoparticles Ultraviolet-visible spectroscopy Gold nanoparticles were initially characterized by UV – vis absorption spectroscopy using a Rayleigh VIS 723-G spectrophotometer to confirm the characteristic spec- trum. The particles were also characterized in relation to the concentration of Au° using the optical density (O.D.) at 400 nm removed from the spectrum, with a validated mathematical treatment [29,30]. The long- itudinal surface plasmonic resonance peak was used to estimate the aspect ratio (AR) [31]. Transmission electron microscopy (TEM) Transmission electron microscopy (TEM) images were obtained using a Tecnai G2–12 electron microscope (SpiritBiotwin FEI, 120 kV). Size distribution analysis and standard deviation were obtained using Image J software (National Institutes of Health [NIH], Bethesda, MD, USA), using TEM images of at least 100 particles. The data obtained were plotted using OriginPro 8.5 for statistical analysis [28]. Zeta potential AuNps and AuNpRSVs were characterized by the zeta potential in an Anton Paar Litesizer 500 device, using an electrophoretic mobility cell for the device model [28]. Infrared spectroscopy Owing to the amphiphilic nature of polyphenols, they can be intercalated with CTAB on the surface of gold nanoparticles when introduced into the synthesis [32,33]. Therefore, through infrared spectroscopy ana- lysis (FTIR), the presence of resveratrol on the surface of gold nanoparticles produced by the seedless method can be evaluated. FTIR spectroscopy was performed using a Shimadzu FTIR 8400 apparatus, and 5 mL of each gold nanoparticle sample was centrifuged, resus- pended in 1 mL of Milli-Q water, and lyophilized. Each lyophilized sample was incorporated into KBr tablets and inserted into the device for readings from 4000 to 500 cm−1, with 200 reading accumulations [28]. 2 P. H. FONSECA DO CARMO ET AL. C. albicans strains We used two strains of C. albicans, a reference strain (C. albicans SC5314) and a strain isolated from the oropharyngeal candidiasis of a patient living with HIV (C. albicans 60) [34]. These strains were cultured on Sabouraud dextrose agar (SDA; Difco, Detroit, MI, USA) at 37°C for 24 h prior to each assay. Experimental groups To study C. albicans, control gold nanoparticles (AuNps) and resveratrol-coated gold nanoparticles (AuNpRSV) were evaluated. Prior to each assay, AuNp and AuNpRSV were centrifuged at 3500 × g at 20°C for 30 min. After this period, at least 95% of the supernatant was removed, and the remaining content was transferred to a new tube. Then, the equivalent volume of the removed supernatant was replaced in distilled water. After this, the stock solution was diluted again to reach the concentration of the working solu- tion. The antifungals fluconazole (FCZ; Sigma-Aldrich, St. Louis, MO), amphotericin B (AMB; Sigma-Aldrich), the surfactant CTAB, and the polyphenol RSV were also included as control in the experiments. Susceptibility of C. albicans planktonic cells to AuNp and AuNpRSV To determine the susceptibility of C. albicans planktonic cells to the gold nanoparticle (AuNp), gold nanoparticle with resveratrol (AuNpRSV), AMB, FCZ and RSV, we employed the broth microdilution method proposed by the Clinical and Laboratory Standards Institute (CLSI) document M27-A3. We used concentrations ranging from 16.00 to 0.01 µg/mL for AMB and FCZ, from 256.00 to 0.25 µg/mL for RSV, from 157.44 to 0.15 µg/ mL for AuNp and AuNpRSV. Concentrations ranging from 350 to 0.68 µM of CTAB were tested. C. albicans cultures previously grown for 24 h at 37°C were suspended in saline, counted using a haemocytometer, and diluted in RPMI-1640 medium (Sigma-Aldrich; with L-glutamine, without glucose and NaHCO3, buffered to pH 7.0 with 0.165 M MOPS) to reach a final concentration of 103 cells/mL. Microplates containing 100 µL of 2-fold serial dilutions of AMB, FCZ, RSV, CTAB, AuNp, and AuNpRSV were inoculated with 100 μL of the adjusted inoculum and incubated at 37°C for 48 h. The minimum inhibitory concentration (MIC) was visually determined as the lowest concentration cap- able of inhibiting 80% of fungal growth for FCZ and 100% for AMB, RSV, CTAB, AuNp, and AuNpRSV. Fungus-free sterility and growth controls were included, and C. parapsilosis ATCC 22,019 was used as a quality control. Additionally, 10 μL was removed from each well with- out visible growth after the MIC assay, cultured on SDA plates, and incubated for 24 hours at 37°C. The minimum fungicidal concentration (MFC) was determined as the lowest concentration of AuNp and AuNpRSV capable of preventing 100% fungal growth after plating. The com- pound was considered fungicidal if the MFC/MIC ratio was ≤ 4 and fungistatic if the ratio was > 4 [35]. Time-kill curves Time-kill experiments were conducted using the RPMI medium as the growth medium. Based on the MIC values, C. albicans SC5314 and C. albicans 60 (103 cells/mL) were exposed to AMB, FCZ, RSV, AuNps, and AuNpRSV at their MIC and 2× MIC. Aliquots were taken at 0, 2, 8, 24, and 48 h after exposure and plated on SDA to determine the colony-forming units (CFU/mL) [36]. Non-treated cells were used as negative controls. Effects of AuNp and AuNpRSV against mature biofilms of C. albicans After the antifungal activity of AuNp and AuNpRSV against C. albicans planktonic was determined, we eval- uated the effects of AuNp and AuNpRSV against mature biofilms. Suspensions of C. albicans SC5314 and C. albicans 60 were counted using a haemocytometer and adjusted to 107 cells/mL. Initially, 100 μL of the standardized suspension was added to microplates containing Yeast Nitrogen Peptone (YNB; Difco) with 100 mm glucose and incu- bated for 90 min at 37°C with shaking. The supernatant was then removed, and each well was washed twice with PBS to remove non-adherent cells, 200 µL of fresh YNB was added, and the microplates were incu- bated for 24 h at 37°C with shaking [37]. After this period, fresh YNB containing AuNp and AuNpRSV at 1× MIC, and 5× MIC was added, and the microplates were incubated for 24 h at 37°C. The untreated group was used for each strain. After treatment, the biofilms were disrupted using an ultrasonic homogenizer (Sonopuls HD 2200, Bandelin Electronic, Berlin, Germany) at 50 W for 30 s. The suspension was plated on SDA, and the plates were incubated at 37°C for 24 h to determine the colony-forming units (CFU/mL). Influence of AuNp and AuNpRSV on C. albicans filamentation In a 24-well culture plate, 2 mL of deionized water was mixed with 10% foetal bovine serum (FBS) (Sigma- Aldrich) and 100 μL of standardized C. albicans SC5314 or C. albicans 60 suspensions (107 viable cells/mL). Groups were treated with 1× and 5× MIC of AuNp or AuNpRSV, VIRULENCE 3 and a control group treated with PBS was used. The plates were incubated at 37°C for 24 h (5% CO2), and then 10 μL of the inoculum was transferred to glass slides. The number of blastoconidia and hyphae was determined by averaging the structure counts per field under a light microscope at 400 × magnification. Four fields were analysed for each slide [38]. Effects of AuNp and AuNpRSV in the production of reactive oxygen species (ROS) and ergosterol levels of C. albicans The endogenous amount of reactive oxygen species (ROS) was measured using a fluorometric assay with a specific probe. C. albicans SC5314 and C. albicans 60 (103 cells/mL) were exposed to AuNPs and AuNpRSV at sub-inhibitory concentrations (½ MIC) diluted in RPMI medium without phenol red. The suspension was incubated for 3 h and 24 h with 2,7-dichlorofluor- escein diacetate (10 µM; DCFH-DA; Life Technologies) to quantify ROS [39]. Fluorescence was measured on a fluorimeter (Synergy 2 SL Luminescence Microplate Reader; Biotek) at excitation and emission wavelengths of 485 and 530 nm, respectively. To determine the effects of AuNp and AuNpRSV on ergosterol levels in C. albicans, SDA plates were sup- plemented with AuNp and AuNpRSV at 1× and 5× the MIC. Then, fungal inoculum (108 CFU) was added, and the plates were incubated at 37°C for 24 h. Fungal mass (20.0 mg) was collected, lysed with an ethanolic solu- tion of potassium hydroxide (25% KOH), mixed for 1 min, and incubated in a water bath at 85°C for 1 h. Sterols were extracted by adding a mixture of n-hep- tane (Sigma-Aldrich) and distilled water (3:1), followed by vigorous homogenization for 3 min. The superna- tant was collected and the ergosterol content was deter- mined by spectrophotometry at 282 nm. A calibration curve using an ergosterol standard (Sigma-Aldrich) was constructed and used to calculate the amount of ergos- terol (µg/mL) [40]. Effect of exposure of C. albicans to AuNp and AuNpRSV under favorable and stress conditions Fungal growth was evaluated by spot testing on SDA (favourable control conditions) and SDA supplemented with different stressors. The cell membrane stressors used were AMB (2.00 µg/mL), FCZ (4.00 µg/mL), and sodium chloride (NaCl; 1.00 M). The cell wall stressors used were Congo red (10 µg/mL) and calcofluor white (CFW; 100 µg/mL). Suspensions of C. albicans SC5314 and C. albicans 60 (108 cells/mL) in RPMI medium were treated with AuNp and AuNpRSV at their MIC values for 24 h. Non-treated groups were also included. After treatment, the suspension was counted using a haemocytometer and adjusted accordingly. Then, 10 µL of C. albicans suspension (104, 103 and 102 cells) was placed on drug-free SDA or SDA supplemen- ted with xenobiotics. Petri dishes were incubated at 37°C for 24 h, and fungal growth was photographed [41,42]. Transmission electron microscopy (TEM) Suspensions of C. albicans SC5314 (108 cells/mL) in RPMI medium were treated with AuNp or AuNpRSV at their MIC values for 24 h. The untreated group was included as a negative control. Then, C. albicans cells were fixed for at least 2 h at room temperature in paraformaldehyde 4.0%, glutaraldehyde 2.5% in caco- dylate buffer 0.1 M, pH 7.2. After fixation, cells were washed in PBS and postfixed in OsO4 1% in cacodylate buffer 0.1 M, pH 7.2 with potassium ferricyanide 1%, and CaCl2 5 mm at room temperature in the dark. The cells were then washed in PBS, dehydrated in acetone, and embedded in Epon. Thin sections were stained with uranyl acetate and lead citrate and observed under a Tecnai G2 Spirit BioTWIN 120 kV (FEI) Transmission Electron Microscope. Toxicity of AuNp and AuNpRSV on Galleria mellonella model The toxicity of AuNp and AuNpRSV was assessed in Galleria mellonella. AuNp and AuNpRSV were injected at different concentrations (1×, 10× and 20× MIC) directly into G. mellonella haemolymph and monitored for 7 days to generate survival curves. Groups that were not injected and those injected with PBS were included as controls. Ten larvae were used for each group. Larvae were considered dead if they did not respond to touch [43]. Statistical analysis Data were analysed in the Prism 6 software (GrapPad Inc., San Diego, CA, USA) using analysis of variance (ANOVA), followed by Tukey’s test. Values of p < 0.05 were considered statistically significant. Results Synthesis and characterization of AuNp and AuNpRSV Both AuNp and AuNpRSV synthesized by the seedless method presented a blue-coloured colloid dispersion (Figure 1a,c). UV-Vis absorption spectroscopy demon- strated the characteristic spectrum of gold nanoparti- cles with two plasmonic bands. AuNP and AuNpRSV 4 P. H. FONSECA DO CARMO ET AL. exhibited a transverse surface plasmon resonance band between 500–520 nm, and a longitudinal surface plas- mon resonance band between 630–650 nm (Figure 1b, d). The theoretical approach revealed an AR value of approximately 2. TEM images demonstrated rod-shaped gold nano- particles (Figure 2) and confirmed an AR of approxi- mately 2, which matches the AR acquired in the UV- Vis spectrum. The medium sizes found were 32.4 ± 3 nm (length) X 15.9 ± 1,8 nm (diameter) for AuNp, and 33.5 ± 1.5 nm (length) X 15.3 ± 1.8 nm (diameter) for AuNpRSV, as shown in sizes distribution histograms (Figure 3). The infrared spectra of CTAB and free resveratrol (Figure 4a), AuNp and AuNpRSV were determined, verifying that the FTIR spectra of AuNp contained only CTAB on its surface, whereas AuNpRSV con- tained a mixture of CTAB and RSV. In the AuNp, we observed stretches at 3017.7 cm−1 that refer to the NH bond of CTAB quaternary ammo- nium group, stretches at 2917.4 and 2849.9 cm−1 indi- cate presence of CH bonds from alkanes group, and 1474.3 cm−1 represent – CH3 stretching at the end of CTAB structure (Figure 4b). In AuNpRSV, we also observed a band at 3243.4 cm−1 due to the presence of the hydroxyl O – H group present in RSV. In these spectra, we found characteristic bands of the aromatic polyphenolic skeleton (stretch C – C) in the region of 1600 to 1400 cm−1, different bands referring to the vibration of the C – H group (1300 to 1000 cm−1), and a C – O connection stretch close to 1200.0 cm−1, indicating the presence of RSV on AuNpRSV produced Figure 1. Visual and UV-Vis characteristics of gold nanoparticles. a) aspects and b) UV-Vis spectrum of synthetized of gold nanoparticles (AuNp). c) aspects and d) UV-Vis spectrum of synthetized of gold nanoparticles with resveratrol (AuNpRSV). VIRULENCE 5 by the seedless method and the exclusive presence of CTAB on AuNps (Figure 4c). Zeta potential values also confirmed the differences in AuNp surfaces, indicating the effective presence of RSV in AuNpRSV. Values were + 35 (s.d. 1.6) mV and + 53 (s.d. 2.0) mV for AuNp and AuNpRSV, respec- tively (Figure 5a,b). AuNp and AuNpRSV exhibited activity against C. albicans planktonic cells In the broth microdilution tests, we found MIC values of 2.46 µg/mL for AuNp and AuNpRSV, > 256.00 µg/mL for RSV, 0.50 µg/mL for FCZ, and 0.12 µg/mL for AMB. MIC of CTAB was 5.46 µM for both strains. MFC values were 4.92 µg/mL for AuNp Figure 2. The gold nanoparticles were rod-shaped. TEM images of (a) gold nanoparticles (AuNp) and (b) gold nanoparticles with resveratrol (AuNpRSV). Figure 3. Gold nanoparticles have similar sizes. Histograms of size distribution (diameter and length) of a and b) gold nanoparticles (AuNp), and c and d) gold nanoparticles with resveratrol (AuNpRSV). 6 P. H. FONSECA DO CARMO ET AL. and AuNpRSV, respectively, with an MFC/MIC ratio of 2, indicating fungicidal activity of AuNp and AuNpRSV against C. albicans strains (Figure 6a). We then evaluated the effects of AuNp and AuNpRSV on C. albicans viability over time. AuNp (1× MIC) completely decreased the viability of C. albicans SC5314 at 24 h and C. albicans 60 at 8 h. AuNpRSV (2× MIC) reduced the total viability of both C. albicans SC5314 and 60 at 8 h. Microbial reductions remained until the end of the experiment (48 h after treatment) (Figure 6b–e). AuNp and AuNpRSV decreased viability of mature biofilms and inhibited the filamentation of C. albicans Based on the C. albicans susceptibility results to AuNp and AuNpRSV, we studied their effects on mature biofilms and filamentation ability of C. albicans. AuNps significantly reduced the biofilm viability of C. albicans SC5314 at 1× MIC and 5× MIC. For C. albicans 60, the viability was reduced only at 5× MIC. AuNpRSV significantly decreased the viability of C. albicans SC5314 and 60 at both concentrations (Figure 7a,b). In relation to filamentation assays, AuNps reduced the filamentation of both C. albicans strains at 1× and 5× MIC. AuNpRSV reduced the number of hyphae at 1× MIC, and completely inhibited filamentation at 5× MIC (Figure 7c,d). AuNp and AuNpRSV reduced ROS production and ergosterol levels of C. albicans ROS production decreased significantly with AuNp treatment for C. albicans 60 at 2 h and for C. albicans SC5314 at 24 h. More specifically, treatment with AuNpRSV reduced ROS for both strains at 2 h and for C. albicans SC5314 at 24 h (Figure 8a,b). In relation to ergosterol levels, both AuNp and AuNpRSV treat- ments reduced the ergosterol content of C. albicans SC5314 at 5× MIC, and for C. albicans 60 at 1× and 5× MIC. Interestingly, Candida cells treated with AuNpRSV had a significantly higher reduction in ergosterol levels than those treated with AuNp (Figure 8c,d). Exposure to AuNp and AuNpRSV increased the susceptibility of C. albicans to cell membrane and cell wall stressors As AuNp and AuNpRSV decreased ergosterol levels in C. albicans, we studied the effect of AuNp and AuNpRSV exposure on C. albicans growth under favourable and stressful conditions. Exposure to AuNps did not affect C. albicans SC5314 on SDA, but slightly reduced fungal growth in cell membrane stressors (AMB, FCZ, and NaCl). However, exposure of C. albicans SC5314 to AuNpRSV significantly reduced fungal growth on SDA and inhibited growth of both cell membrane and cell wall stressors. Figure 4. Infrared spectroscopy analysis (FTIR) confirmed synthesis of gold nanoparticles with resveratrol. Infrared spectrum of a) CTAB and free resveratrol, and of b) gold nanoparticles (AuNp) and c) gold nanoparticles with resveratrol (AuNpRSV). VIRULENCE 7 In relation to C. albicans 60, exposure to AuNp reduced cell viability on SDA, as well as on SDA supplemented with cell wall and, mainly, cell mem- brane stressors (AMB, FCZ, and NaCl). Exposure of C. albicans 60 to AuNpRSV decreased the viability of SDA, AMB, RED, and CFW, but the reduction was marked in the FCZ and NaCl stressors (Figure 9). Exposure to AuNpRSV affected the morphology of C. albicans cells C. albicans SC5314 cells (non-treated group) displayed well-conserved morphological features with a typical and distinctive membrane and cell wall (Figure 10a). In contrast, cells exposed to AuNps were generally Figure 5. Zeta potential of gold nanoparticles. Zeta potential (mV) of a) gold nanoparticles (AuNp) and b) gold nanoparticles with resveratrol (AuNpRSV). 8 P. H. FONSECA DO CARMO ET AL. enlarged, with alterations in the cell membrane and cell wall, which showed an increase in thickness and loss of electron-dense characteristics. TEM images also revealed a thin deposition of AuNps, represented by an electrodense line between the cell wall and cell membrane (Figure 10b). Interestingly, exposure to AuNpRSV enhanced cell damage. We observed fungal cells with significant cell wall changes, represented by enlargement and retraction of the cell membrane (Figure 10c). TEM images were used to measure cell wall width. Non-treated fungal cells exhibited a width of 120 nm, whereas cells treated with AuNpRSV were 250 nm in width, confirming the action of AuNpRSV on the cell membrane of C. albicans. AuNp and AuNpRSV maintained full viability of G. mellonella larvae The survival of G. mellonella after inoculation with AuNp or AuNpRSV was determined to assess the Figure 6. Susceptibility of C. albicans strains to AuNp and AuNpRSV. a) minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and MFC/MIC ratio of gold nanoparticles (AuNp), resveratrol (RSV), gold nanoparticles with resveratrol (AuNpRSV), amphotericin b (AMB) and fluconazole (FCZ) against C. albicans strains determined by the broth microdilution method. Time-kill curves for C. albicans SC 5314 at B) 1× MIC and c) 2× MIC (MFC), and for C. albicans 60 at d) 1× MIC and e) 2× MIC (MFC) during 48 hours of treatment. * significant difference compared to non-treated (NT) at each established time (p < 0.05). AMB: amphotericin B; AuNp: gold nanoparticles; RSV: resveratrol; AuNpRSV: gold nanoparticles with resveratrol; FCZ: fluconazole; MFC: minimum fungicidal concentration; MIC: minimum inhibitory concentration; NG: no growth; NT: non-treated. VIRULENCE 9 Fi gu re 7 . G ol d na no pa rt ic le s re du ce d th e fil am en ta tio n an d vi ab ili ty o f C. a lb ic an s bi of ilm s. F un ga l v ia bi lit y of a ) C. a lb ic an s SC 5 31 4 an d b) C . a lb ic an s 60 b io fil m s no t tr ea te d (N T) a nd tr ea te d w ith g ol d na no pa rt ic le s (A uN p) a nd g ol d na no pa rt ic le s w ith r es ve ra tr ol ( Au N pR SV ) at 1 × a nd 5 × M IC . P er ce nt ag e of h yp ha e an d bl as to co ni di a of c ) C. a lb ic an s SC 5 31 4 an d d) C. a lb ic an s 60 n ot t re at ed a nd t re at ed w ith A uN p an d Au N pR SV a t 1× a nd 5 × M IC . * : s ig ni fic an t di ffe re nc e co m pa re d to n on -t re at ed ( N T) . # : s ig ni fic an t di ffe re nc e co m pa re d to A uN p (p < 0 .0 5) . A uN p: g ol d na no pa rt ic le s; A uN pR SV : r es ve ra tr ol -c oa te d go ld n an op ar tic le s; N T: n on -t re at ed . 10 P. H. FONSECA DO CARMO ET AL. Figure 8. Treatment with gold nanoparticles reduced ROS production and ergosterol levels. Production of reactive oxygen species (ROS) of C. albicans SC5314 (SC5314) and C. albicans 60 (Ca60) not treated and treated with gold nanoparticles (AuNp), gold nanoparticles with resveratrol (AuNpRSV) and hydrogen peroxide (HP) after a) 2 and b) 24 hours. Ergosterol (µg/mL) levels of c) C. albicans SC5314 and d) C. albicans 60 not treated (NT) and treated with AuNp and AuNpRSV at 1× and 5× MIC, and with fluconazole (FCZ). *: significant difference compared to non-treated (NT). #: significant difference compared to AuNp (p < 0.05). AuNp: gold nanoparticles; AuNpRSV: resveratrol-coated gold nanoparticles; NT: non-treated. Figure 9. Exposure of C. albicans to AuNpRSV affected fungal growth in cell membrane stressors. Spot tests of C. albicans SC5314 and C. albicans 60 at 104, 103 and 102 cells not exposed and exposed to gold nanoparticles (AuNp) and to gold nanoparticles with resveratrol (AuNpRSV), and plated on sabouraud dextrose agar (SDA) pure, and supplemented with amphotericin B (AMB) fluconazole (FCZ), sodium chloride (NaCl), Congo red (RED) and calcofluor white (CFW). VIRULENCE 11 toxicity of the nanoparticles. Both nanoparticles main- tained 100% viability of G. mellonella larvae at 1×, 10× and even 20× MIC (Figure 11). Discussion Gold nanoparticles have been widely studied for the treatment of infectious diseases because of their potential to deliver drugs, including antifungals [21]. Associated with an intrinsic antifungal activity, gold nanoparticles present good physicochemical properties, chemical resistivity, ease of synthesis, and minimal size [44,45]. In this study, we synthesized and characterized gold nanoparticle rod-shaped with resveratrol. Gold atoms tended to be deposited on the tips of the nanostructure Figure 10. C. albicans cell membrane was damaged after exposure to gold nanoparticles. Transmission electronic microscopy (TEM) images of a) cells not treated, and treated with b) gold nanoparticles (AuNp) and c) gold nanoparticles with resveratrol (AuNpRSV). Black arrows indicate a thin deposition of AuNp between of cell membrane and cell wall. Figure 11. AuNp and AuNpRSV were not toxic to G. mellonella larvae. Survival percentage of galleria mellonella larvae inoculated with a) gold nanoparticles (AuNp) and b) gold nanoparticles with resveratrol (AuNpRSV) at MIC, 10× MIC and 20× MIC. MIC: minimum inhibitory concentration. 12 P. H. FONSECA DO CARMO ET AL. during the growth process, mainly because of the greater binding affinity of CTAB for the lateral facets of the nanostructure. In addition, green synthesis using phenolic compounds, such as RSV, increases the affinity of CTAB for the lateral facets of gold nanorods, facilitating the maintenance of the nanorod structure [46,47]. Although the use of CTAB during the synthesis may be a concern for the biomedical application of nanoparticles [48], washing and dilution processes provide a reduction of this compound to residual concentrations [49], that do not show activity against C. albicans. The synthesis method was successful, according to the characterization data. The seedless method effec- tively addresses the limitations inherent to conven- tional approaches, such as variable reproducibility, prolonged reaction times, and inefficient scaling [50,51]. An enhancement strategy for this synthesis method involves the introduction of phenolic com- pounds, such as resveratrol, as auxiliary reducers. The inclusion of these substances has streamlined particle production, resulting in excellent reproduci- bility, uniform size characteristics, high yield, and a capacity for large-volume production [28,52]. Moreover, the utilization of resveratrol as a reducing agent aligns with the principles of green synthesis and offers several advantages. This includes the use of reduced amounts of potentially harmful chemicals and production of more stable and monodispersed nanoparticles [49,52,53]. The UV-Vis scanning spectra show the characteristic absorption profiles of AuNp and AuNpRSV, with two distinct plasmon resonance bands. The sizes of the nanoparticles were confirmed using AR analysis and TEM images. Since particle suspensions with zeta potential higher than | 30 | mV is considered stable [54], we obtained good stability for the AuNp (+35 mV) and AuNpRSV (+53 mV) for AuNp and AuNpRSV, respectively. AuNpRSV exhibited a higher zeta potential than AuNp, which confirmed its enhanced colloidal stability. XPs oxygen signals increased significantly in AuNpRSV analysis when compared to AuNp, suggesting the presence of RSV on the nanorods surface, and corroborating the results of the FTIR spectra [28,55]. In addition, both analyzes indicated a low amount of CTAB, mainly in AuNpRSV. Altogether, these data suggest that a good carrier sys- tem for RSV was constructed using a green synthesis method [28,56]. The FTIR spectra associated with the XPS data confirmed the presence of RSV on the nano- particles surfaces [55], suggesting that a good carrier system for RSV was constructed using a green synthesis method [28,56]. Green synthesis is an eco-friendly and inexpensive method that uses natural compounds or microbial metabolites as the reducing and stabilizing agents for gold nanoparticles [25,57]. Furthermore, nanoparticles produced by green synthesis can reduce toxicity [58]. In relation to antifungal activity, we verified that resveratrol was not able to inhibit fungal growth at the highest concentration analysed in this study (256 µg/ mL). Indeed, previous studies have demonstrated that concentrations higher than 2000 µg/mL are necessary to reduce fungal growth by 20 % [59,60]. Resveratrol is a phenolic compound present in natural food products, including grapes, wine, peanuts, and berries [61,62]. Furthermore, the low solubility of resveratrol in water and its rapid clearance make it difficult to maintain its bioeffective concentrations in the blood and target tis- sues of humans [63,64]. Nanoformulation has been pro- posed as a strategy to overcome the physicochemical limitations of resveratrol [65]. The antifungal effects of AuNps and AuNpRSVs were studied against C. albicans. AuNp and AuNpRSV inhibited C. albicans growth at 2.46 µg/mL and no fungal burden was detected after 8 h of treat- ment. In addition, AuNp and AuNpRSV exhibited fun- gicidal activities against C. albicans at low concentrations (4.92 ug/mL). These results indicated that AuNps had an intrinsic effect against C. albicans because the MIC and MFC values did not change after functionalization with resveratrol. Ultra Performance Liquid Chromatography (UPLC) analyzes calculated 43.45 μg/mL of RSV on AuNpRSV [49]. However, after the successive washing and dilution processes, probably the final concentration of RSV was minimal. Nevertheless, it contributes to the improved perfor- mance of the constructed nanosystem by providing greater stability and enabling synthesis in large volumes. Nidhin et al. [66] also observed an inhibitory effect of non-functionalized gold nanoparticles against C. albicans cells at 0.5 mm. By contrast, Yu et al. [14] found no inhibitory effect of gold nanoparticles (160 ppm) on C. albicans growth and hyphal development. In studies with silver nanoparticles (AgNps), Lee et al. [67] and Ahamad et al. [68] demonstrated that treat- ment of C. albicans cells caused a delay in the G1 phase and directly affects the cell cycle. In this phase, the synthesis of RNA, proteins and cellular organelles was observed, that are fundamental structures for fungal replication and growth [69]. Although in our study the growth of C. albicans was affected after treatment with AuNp and especially AuNpRSV, the effects of treatment with gold nanoparticles on the cell cycle of C. albicans need further investigation. VIRULENCE 13 After the assays in planktonic cells, we studied AuNp and AuNpRSV against mature biofilms and filamenta- tion of C. albicans. The ability to change morphology and form biofilms is the chief virulence factor of C. albicans and is a central strategy in its pathogenesis [8,70]. Atiencia-Carrera et al. [71] studied the mortality rate in Candida-related bloodstream infections and observed that 70% were biofilm-associated. C. albicans biofilms are complex structures encapsulated in a self- secreted polymeric extracellular matrix that protects cells from the surrounding environment and provides resistance to conventional antifungal therapy and host immune system components [10,72]. In addition, C. albicans forms biofilms on biotic and abiotic sur- faces, including host tissues, medical devices, dentures, and catheters, which contribute to recurrent candidiasis and represent a challenge in antifungal therapy [27,70,71]. Therefore, it is important that potential antifungal agents have activity against both the plank- tonic and biofilm stages of C. albicans. In our study, AuNp reduced biofilm viability and C. albicans filamentation at 5× MIC, whereas AuNpRSV decreased viability and filamentation at 1× MIC. Therefore, decreased biofilm viability may be associated with a reduction in the number of hyphae after treatment with gold nanoparticles. Yu et al. showed that non-functionalized gold nanoparticles strongly inhibited C. albicans biofilm formation, although no alterations in the expression levels of hypha-specific genes (HWP1, ECE1 and ALS3) were detected [14]. In other studies, gold nanoparticles func- tionalized with fucoidan and β-caryophyllene were able to inhibit polymicrobial biofilms of C. albicans, Staphylococcus aureus and Streptococcus mutans [73], C. albicans and S. aureus, respectively [74], demonstrat- ing the broad-spectrum antimicrobial activity of gold nanoparticles. Regarding the effects of AuNp and AuNpRSV on ROS production in Candida cells, we observed decreased ROS levels after treatment. Numerous stu- dies have suggested that ROS is intrinsically related to apoptosis in C. albicans [39,75,76]. Indeed, ROS are short-lived and highly reactive molecules, and their overexpression causes damage to proteins, nucleic acids, membranes, and organelles, triggering cell death processes including apoptosis [77,78]. This effect was observed by Ahamad et al. [68] and Lee et al. [67] when treating C. albicans cells with AgNps. Flow cytometry analyzes revealed that the treatment with AgNps increased cell membrane permeability and reduced the integrity of fungal DNA, resulting in nuclear abnormalities associated with ROS production and apoptosis. However, in this study, we observed reduced ROS production after treatment with AuNp and AuNpRSV, suggesting an ROS-independent apop- totic pathway. Similarly, Seong and Lee [79] demon- strated a pathway in which ROS signalling did not influence the mechanisms of AuNp-induced cell damage. In their study, the mode of action of AuNps was related to DNA damage, mitochondrial dysfunc- tion, and apoptosis via metacaspase activation. However, further investigation is necessary to confirm the mechanism of action of the gold nanorods on ROS production. In our study, we also verified that AuNp and AuNpRSV reduced ergosterol levels in C. albicans cells after treatment with 5× MIC. Interestingly, this reduction was enhanced by treatment with AuNpRSV. Ergosterol is the major sterol in the fungal plasma membrane, contributes to a variety of cellular func- tions, and is distributed in both the cell membrane and intracellular endomembrane components [80]. Ergosterol is the target of polyene antifungals, and its biosynthetic pathway is the target of allylamines and azole antifungals [81]. However, the effects of resvera- trol treatment on C. albicans ergosterol levels have not yet been explored. Sun et al. [82] observed that the combination of resveratrol and fluconazole reduced ergosterol levels and increased lanosterol content, a precursor of ergosterol, in C. albicans. In addition, studies on other polyphenols, such as honokiol and curcumin, have proven their ability to reduce ergosterol levels in C. albicans [80,83]. Interestingly, C. albicans cells exposed to AuNpRSV showed a reduced ability to grow on cell membrane stressors (AMB, FCZ, and NaCl), as well as on cell wall stressors (CFW and RED). In contrast, C. albicans cells treated with AuNps grew normally in the xenobiotics evaluated. Additionally, TEM images confirmed the interaction of AuNp and AuNpRSV with the cell mem- brane and revealed significant damage to the treated cells, mainly characterized by enlargement of the cell wall and retraction of the plasma membrane. Although inorganic nanoparticles exhibit antifungal effects, their cytotoxicity remains a concern [84]. In a previous study by our group [49], AuNps and AuNpRSVs showed reduced cytotoxicity in mammalian cells. The cytotoxic concentrations for 50% of the cells (CC50) were 50 µg/mL and higher than 50 µg/mL for AuNp and AuNpRSV, respectively. We then evaluated the toxicity of both types of nanoparticles using the G. mellonella model. This model provides a fast and convenient means of studying in vivo toxicity of poten- tial antifungal compounds. In addition, the results showed a strong correlation with the mammalian hosts [85]. In our study, we observed that AuNp and 14 P. H. FONSECA DO CARMO ET AL. AuNpRSV did not affect the survival of G. mellonella larvae at concentrations up to 49.20 µg/mL, 20 times the MIC value, confirming their reduced toxicity in vivo. In summary, we synthesized and characterized two stable gold nanorods, AuNps and AuRSVs. AuNps exhibited intrinsic activity against planktonic cells and biofilms of C. albicans, reducing fungal filamentation, ROS production, and ergosterol levels. However, the antifungal effects of AuNpRSV were enhanced. In addi- tion to its fungicidal activity on C. albicans cells and biofilms, treatment with AuNpRSV significantly reduced the levels of ergosterol, and this effect was observed in C. albicans cells observed in the TEM images. Importantly, AuNp and AuNpRSV exhibited antifungal effects with no in vivo toxicity, reinforcing their potential as agents for treating C. albicans infections. These results suggest that the gold nanoparticles produced by green synthesis with resveratrol can be a valuable approach for antifungal therapy against C. albicans. Acknowledgements We thank Professor Anita Shukla of Brown University for her support. PHFC was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP (2022/06127-1) and Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (152378/2022-7). JCJ was supported by the FAPESP (2022/15548-0). Disclosure statement No potential conflict of interest was reported by the author(s). Funding This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP [2022/15548-0 and 2022/06127-1] and Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq [152378/2022-7]. Author’s Contributions Conception and design: PHFC, ACPL, JCJ. Methodology: PHFC, ACPL, MTG, NSS, DAS, EM and JCJ. Analysis and interpretation of the data: PHFC, ACP, MTG, NSS, DAS, JCJ. Financial support and supervision: JCJ. Drafting of the paper: PHFC and ACPL. All authors contributed to revision of the manuscript and final approval of the version to be published. Data availability statement The data that support the findings of this study are openly available at https://repositorio.unesp.br/items/4645d920- 13ee-49ef-b11f-3278b0e81a74. ORCID Paulo Henrique Fonseca Do Carmo http://orcid.org/0000- 0003-3186-885X Anna Carolina Pinheiro Lage http://orcid.org/0000-0003- 1663-369X Maíra Terra Garcia http://orcid.org/0000-0002-1193-2909 Newton Soares da Silva http://orcid.org/0000-0001-6452- 9278 Daniel Assis Santos http://orcid.org/0000-0002-1108-5666 Eleftherios Mylonakis http://orcid.org/0000-0002-4624- 0777 Juliana Campos Junqueira http://orcid.org/0000-0001- 6646-6856 References [1] Nobile CJ, Johnson AD. Candida albicans biofilms and human disease. Annu Rev Microbiol. 2015;69(1):71–92. doi: 10.1146/annurev-micro-091014-104330 [2] Talapko J, Juzbašić M, Matijević T, et al. Candida albicans—the virulence factors and clinical manifesta- tions of infection. J Fungi. 2021;7(2):79. doi: 10.3390/ jof7020079 [3] Vallabhaneni S, Mody RK, Walker T, et al. The global burden of fungal diseases. Infect Dis Clin North Am. 2016;30(1):1–11. doi: 10.1016/j.idc.2015.10.004 [4] Wuyts J, Van Dijck P, Holtappels M, et al. 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FONSECA DO CARMO ET AL. https://doi.org/10.2147/IJN.S205736 https://doi.org/10.2147/IJN.S205736 https://doi.org/10.3389/fmicb.2021.741493 https://doi.org/10.3389/fmicb.2021.741493 https://doi.org/10.1111/mmi.14097 https://doi.org/10.1111/jam.14949 https://doi.org/10.1371/journal.pone.0263522 https://doi.org/10.1099/jmm.0.045054-0 https://doi.org/10.3390/md21020123 https://doi.org/10.3390/antibiotics12040726 https://doi.org/10.1128/spectrum.03183-22 https://doi.org/10.1371/journal.pone.0172228 https://doi.org/10.1016/j.bbamcr.2016.09.012 https://doi.org/10.1016/j.bbamcr.2016.09.012 https://doi.org/10.1093/mmy/myac058 https://doi.org/10.1093/mmy/myac058 https://doi.org/10.1016/j.micres.2017.11.003 https://doi.org/10.1016/j.micres.2017.11.003 https://doi.org/10.4014/jmb.2008.08019 https://doi.org/10.4014/jmb.2008.08019 https://doi.org/10.1016/S0966-842X(03)00117-3 https://doi.org/10.1016/j.ab.2004.06.038 https://doi.org/10.2741/e451 https://doi.org/10.3390/antibiotics10121545 https://doi.org/10.3390/antibiotics10121545 Abstract Introduction Material and methods Chemicals Green synthesis of gold nanoparticles Characterization of gold nanoparticles Ultraviolet-visible spectroscopy Transmission electron microscopy (TEM) Zeta potential Infrared spectroscopy C.albicans strains Experimental groups Susceptibility of C.albicans planktonic cells to AuNp and AuNpRSV Time-kill curves Effects of AuNp and AuNpRSV against mature biofilms of C.albicans Influence of AuNp and AuNpRSV on C.albicans filamentation Effects of AuNp and AuNpRSV in the production of reactive oxygen species (ROS) and ergosterol levels of C.albicans Effect of exposure of C.albicans to AuNp and AuNpRSV under favorable and stress conditions Transmission electron microscopy (TEM) Toxicity of AuNp and AuNpRSV on Galleria mellonella model Statistical analysis Results Synthesis and characterization of AuNp and AuNpRSV AuNp and AuNpRSV exhibited activity against C.albicans planktonic cells AuNp and AuNpRSV decreased viability of mature biofilms and inhibited the filamentation of C.albicans AuNp and AuNpRSV reduced ROS production and ergosterol levels of C.albicans Exposure to AuNp and AuNpRSV increased the susceptibility of C.albicans to cell membrane and cell wall stressors Exposure to AuNpRSV affected the morphology of C.albicans cells AuNp and AuNpRSV maintained full viability of G.mellonella larvae Discussion Acknowledgements Disclosure statement Funding Author’s Contributions Data availability statement References