metadata Paper: ADRENERGIC SIGNALING AND PERIODONTITIS: INSIGHTS ON INNATE IMMUNITY AND Porphyromonas gingivalis VIRULENCE IN AN INVERTEBRATE MODEL Authors: Renata Mendonça Moraes, Maíra Terra Garcia, Fabio Stossi, Patrícia Pimentel de Barros, Juliana Campos Junqueira, Ana Lia Anbinder Porphyromonas gingivalis Pg or P. gingivalis Galleria mellonella G. mellonella Isoproterenol ISO Norepinephrine NE Octopamine OCT PgISO Group in which P. gingivalis was cultivated with Isoproterenol in the broth PgNOR or PgNE Group in which P. gingivalis was cultivated with norepinephrine in the broth CT cycle threshold Circulating hemocytes Cells that are freely circulating in the insect hemolymph Sessile hemocytes Cells that are attached to internal organs of the larvae Pg susceptibility Susceptibility of G. mellonella to defferent concentrations of P. gingivalis cultivated in agar Healthy index Days PBS 107UFC/mL 108UFC/mL Day 1 Day2 Day3 Day4 Day5 Day6 Day7 3 1 1= dead larvae PBS 10 9 9 9 10 10 10 4 1 0= alive larvae 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 9 7 0 0 0 0 0 7 0 10 10 6 0 0 0 0 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 7 0 10 10 10 10 10 10 10 4 1 107 UFC/mL 6 6 4 0 0 0 0 4 1 6 6 6 4 0 0 0 5 1 7 6 6 5 0 0 0 5 1 7 6 6 6 0 0 0 5 1 7 6 6 6 6 0 0 5 1 7 6 6 6 6 0 0 5 1 7 6 6 6 6 6 6 5 1 7 7 7 7 6 6 6 6 1 7 8 7 7 6 6 6 6 1 7 7 7 7 6 6 6 6 1 6.5 7 8 3 0 0 0 6 1 6.5 7 6 3 0 0 0 7 0 6.5 5 3 5 0 0 0 7 0 6 6 5 6 6 0 0 7 0 6 5 3 4 5 0 0 7 0 6 6 3 4 7 6 6 7 0 6 5 6 0 0 0 0 7 0 6 6 5 3 7 6 6 7 0 6 6 4 5 7 6 6 7 0 6 6 7.5 6.5 7 6 6 1 1 108 UFC/mL 0 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0 1 1 5 0 0 0 0 0 0 2 1 5 0 0 0 0 0 0 2 1 5 0 0 0 0 0 0 2 1 6 4 0 0 0 0 0 2 1 6 4 0 0 0 0 0 2 1 6 4 0 0 0 0 0 2 1 6 4 0 0 0 0 0 2 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 3 1 4 0 0 0 0 0 0 3 1 4 0 0 0 0 0 0 3 1 4 0 0 0 0 0 0 3 1 4 0 0 0 0 0 0 4 1 4 4 3 0 0 0 0 4 1 4 6 3 0 0 0 0 4 1 4 6 4 3 0 0 0 ISO susceptibility Susceptibility of G. mellonella to different concentrations of Isoproterenol- Healthy index and survival curve Susceptibility ISOPROTERENOL Days PBS 10pM 50pM 100pM 500pM 1nM 10nM 100nM 1uM 10uM 100uM 1= dead larvae Healthy index 5 0 0= alive larvae PBS 10pM 50pM 100pM 500pM 1nM 10nM 100nM 1uM 10uM 100uM 5 0 Day 1 9 9 9.5 8.5 9 9 9 10 9 9 9 5 0 9 10 9.5 9.5 9.5 9 9 10 9 10 10 5 0 9 10 9.5 9.5 9.5 10 10 10 9 10 10 5 0 9 10 9.5 9.5 10 10 10 10 9 10 10 5 0 9 10 10 9 10 10 10 10 8 10 10 5 0 9 10 10 10 10 10 10 10 10 10 10 5 0 9 10 10 10 10 10 10 10 10 10 10 5 0 9 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 9 10 10 10 10 10 10 5 0 10 10 10 10 9 10 10 10 10 10 10 5 0 9.5 9 9 9 8 8 9 8 8.5 8.5 9 5 0 9.5 9 9 9 8 8 8.5 8 8.5 8.5 10 5 0 9.5 9 9 10 9 9.5 9.5 8 8.5 10 8.5 5 0 9.5 10 10 10 10 9.5 9.5 9.5 9 8 9 5 0 9.5 10 10 10 10 9.5 9.5 10 9 9 10 5 0 9.5 10 10 10 10 9.5 9.5 10 9 9.5 10 5 0 9.5 9 10 10 10 9.5 9.5 10 10 9.5 10 5 0 9.5 9 10 10 10 9.5 9.5 9 10 10 10 5 0 9.5 9 10 10 10 9.5 9.5 9 10 9 10 4 1 9.5 9 10 10 10 9.5 9.5 9 10 10 10 5 0 Day 2 9 10 9 8 10 9 9 10 0 0 10 5 0 9 10 9 9.5 10 9 10 10 9 9.5 10 5 0 9 10 9.5 10 10 10 10 10 10 9.5 10 5 0 9 10 10 10 10 10 10 10 10 10 10 5 0 9.5 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 8 9 9 8.5 8.5 9 8 8.5 8 8 8 5 0 8 10 9 9.5 9.5 10 8.5 8.5 8 8 9 5 0 9 10 9 9 9 9 8 8.5 8 8.5 8 5 0 9 10 10 9 9 10 8.5 9.5 8.5 8.5 9 5 0 10 10 10 9 9 9 8.5 9 10 8.5 8.5 5 0 10 10 10 9 9 10 9.5 9 9.5 8.5 9 5 0 10 10 10 10 10 9 9.5 10 9.5 10 9.5 5 0 10 10 10 10 10 9 10 10 10 10 10 5 0 10 10 10 10 10 9 10 10 10 10 10 5 0 10 10 10 10 10 9 10 10 9 10 10 5 0 Day 3 9 10 9 9 9 10 9 9 0 0 9 5 0 9 10 10 9 10 10 10 10 8 9 9 5 0 9 10 10 9.5 10 10 10 10 9 9 9 5 0 10 10 10 10 10 10 10 10 10 9 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 9.5 10 10 10 10 10 10 10 10 10 10 5 0 8.5 10 10 10 10 10 10 10 10 10 10 5 0 9 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 9 8 9 8.5 9.5 9 8 8.5 9.5 8.5 0 5 0 8 9 9 9 9.5 9 9 9.5 9 8.5 8.5 5 0 8 9 9 9 9 9 9 9 8.5 9 9.5 5 0 9.5 9 9 10 9 10 9 9 10 9 10 5 0 9.5 9 10 10 10 10 9 9 10 9 10 5 0 10 10 10 10 10 10 9 9 10 9 8.5 5 0 9 10 10 10 10 10 9 9 10 10 9 5 0 10 10 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 Day 4 9 9 9 8 9 9 8 9 0 0 9 5 0 9 9 9 9 10 9 9 9 9 10 9 5 0 9 10 9 9 10 10 9 9 9 10 9 5 0 9 10 9 10 10 10 9 10 10 10 10 5 0 9.5 10 9 10 10 10 10 10 10 10 10 5 0 9.5 10 9 10 10 10 10 10 10 10 10 5 0 9.5 10 9 10 10 10 10 10 10 10 10 5 0 10 10 9 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 8.5 0 10 9 8 8 7 9 9 9 0 5 0 8.5 9 10 9 9.5 10 9 9 9 9 8 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 10 9 10 9 9 9 9 9 5 0 9 9 9 10 9 10 10 10 9 9 10 5 0 9 9 9 10 9 9 10 10 10 9 10 5 0 9 9 9 10 9 9 10 10 10 9 10 5 0 9 9 9 10 9 9 10 10 10 10 10 5 0 Day 5 8 8 9 8 9 8 9 8 0 0 8 5 0 8 9 8 9 9 9 9 8 8 9 8 5 0 10 10 9 9 9 9 9 9 9 9 8 5 0 10 10 9 9 9 10 9 9 9 9 9 5 0 10 10 10 10 10 10 9 9 9 9 9 5 0 10 10 10 10 10 10 9 10 9 10 9 5 0 10 10 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 10 10 5 0 8.5 0 10 9 8 8 7 9 9 9 0 5 0 8.5 9 10 9 9.5 10 9 9 9 9 8 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 9 10 10 9 9 9 9 9 5 0 9 9 9 10 9 10 9 9 9 9 9 5 0 9 9 9 10 9 10 10 10 9 9 10 5 0 9 9 9 10 9 9 10 10 10 9 10 5 0 9 9 9 10 9 9 10 10 10 9 10 5 0 9 9 9 10 9 9 10 10 10 10 10 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 2 1 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 2 1 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 4 1 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 ISO hemocytes 30 min Number of circulating hemocytes 30 min after the injection of different concentrations of Isoproterenol or PBS (Control) Isoproterenol concentrations- Raw data PBS 10pM 50pM 100pM 500pM 1nM 10nM 100nM 1uM 10uM 100uM 25,500,000 21,250,000 25,500,000 47,500,000 22,750,000 37,750,000 17,250,000 24,250,000 46,750,000 28,250,000 31,750,000 23,500,000 17,750,000 16,500,000 36,250,000 28,250,000 36,500,000 31,000,000 42,000,000 37,750,000 10,750,000 13,750,000 21,750,000 29,750,000 8,500,000 17,000,000 32,250,000 22,750,000 37,750,000 34,250,000 21,250,000 42,000,000 18,000,000 22,500,000 25,500,000 12,000,000 55,500,000 19,500,000 27,000,000 26,500,000 27,250,000 9,250,000 36,250,000 18,250,000 26,750,000 25,500,000 21,500,000 52,500,000 29,750,000 17,000,000 19,000,000 24,000,000 19,250,000 27,500,000 22,750,000 18,000,000 11,750,000 9,250,000 22,000,000 28,000,000 35,000,000 29,250,000 13,250,000 14,000,000 32,250,000 5,750,000 10,750,000 21,750,000 29,000,000 37,250,000 39,500,000 32,750,000 24,250,000 23,250,000 23,000,000 17,500,000 7,750,000 7,750,000 24,000,000 17,500,000 48,750,000 23,500,000 22,500,000 23,750,000 8,000,000 14,000,000 9,250,000 25,000,000 14,000,000 28,750,000 9,500,000 25,750,000 35,750,000 28,750,000 26,750,000 20,750,000 11,750,000 11,500,000 5,500,000 20,000,000 22,750,000 14,000,000 40,750,000 49,500,000 4,500,000 16,250,000 2,500,000 13,250,000 27,250,000 8,250,000 8,250,000 12,750,000 21,000,000 38,750,000 22,500,000 10,000,000 29,500,000 7,750,000 22,500,000 10,250,000 22,500,000 14,250,000 5,500,000 16,000,000 17,250,000 12,000,000 11,750,000 14,500,000 5,250,000 750,000 3,000,000 29,250,000 19,000,000 17,000,000 15,500,000 19,500,000 26,750,000 9,750,000 17,000,000 7,000,000 5,500,000 18,750,000 16,750,000 21,750,000 4,000,000 7,750,000 48,250,000 15,750,000 8,750,000 15,250,000 12,000,000 15,250,000 17,000,000 5,250,000 21,000,000 16,750,000 20,250,000 52,250,000 28,000,000 9,250,000 10,500,000 17,750,000 17,000,000 7,500,000 4,500,000 18,000,000 8,250,000 5,750,000 45,750,000 43,750,000 10,250,000 24,500,000 14,000,000 9,500,000 19,500,000 3,750,000 3,750,000 2,250,000 8,250,000 21,750,000 29,750,000 26,750,000 12,250,000 5,750,000 5,750,000 5,250,000 3,500,000 18,250,000 6,250,000 14,750,000 32,000,000 12,750,000 13,000,000 6,000,000 3,500,000 24,250,000 6,750,000 2,250,000 1,500,000 2,500,000 13,750,000 18,750,000 55,000,000 28,500,000 11,500,000 17,000,000 14,750,000 13,000,000 1,500,000 7,750,000 1,750,000 3,500,000 44,000,000 29,750,000 17,250,000 6,750,000 14,500,000 11,250,000 9,500,000 4,750,000 ISO hemocytes 90 min Number of circulating hemocytes 90 min after the injection of different concentrations of Isoproterenol or PBS (Control) Isoproterenol concentrations- raw data PBS 10pM 50pM 100pM 500pM 1nM 10nM 100nM 1uM 10uM 100uM 33250000 23750000 33250000 27500000 38500000 12250000 10500000 16250000 7500000 15500000 15750000 21750000 21500000 36000000 27250000 24750000 11500000 30250000 15000000 7750000 21500000 9250000 21000000 18000000 39000000 37000000 35500000 13250000 15000000 12000000 9500000 20250000 6500000 21750000 35250000 34000000 36750000 37500000 7250000 28500000 11500000 4250000 28500000 11750000 23000000 15500000 33000000 39000000 28500000 16750000 17000000 9750000 15500000 17500000 5750000 22250000 34000000 19250000 30500000 27000000 27250000 8500000 28500000 7500000 11750000 6750000 26250000 26250000 9250000 39500000 35500000 17500000 9750000 22500000 8250000 6750000 15500000 8000000 23250000 9250000 24750000 29750000 32250000 7500000 5500000 8000000 11500000 14750000 7000000 16500000 14250000 26500000 21750000 22750000 23250000 4250000 7750000 8000000 5000000 13750000 8750000 7250000 21750000 28750000 17250000 20750000 38750000 6750000 8000000 7000000 26750000 12750000 7750000 23750000 20000000 26250000 19000000 29500000 25250000 26500000 31500000 18000000 8250000 7500000 28000000 14250000 14750000 30250000 29750000 21250000 24500000 17750000 12750000 6500000 9500000 18250000 24250000 17000000 16500000 18750000 27500000 29000000 27250000 21750000 24500000 11750000 20000000 16500000 6750000 25750000 16500000 18750000 7500000 24750000 15750000 16500000 19000000 14000000 15750000 21250000 17250000 21000000 14500000 9250000 26000000 21750000 12750000 6500000 18750000 18250000 24000000 17750000 21250000 33000000 13250000 16750000 17500000 22500000 36250000 17500000 28000000 36500000 18750000 23750000 25250000 25500000 29750000 28750000 37250000 14750000 34000000 31250000 23750000 15750000 13250000 20750000 6250000 23500000 11750000 21250000 28750000 18750000 17250000 16500000 11500000 7750000 26750000 20250000 28250000 14000000 11000000 21750000 21000000 19750000 20250000 9250000 12250000 20750000 24250000 17500000 ISO hemocytes 180 min Number of circulating hemocytes 180 min after the injection of different concentrations of Isoproterenol or PBS (Control) Isoproterenol concentrations PBS 10pM 50pM 100pM 500pM 1nM 10nM 100nM 1uM 10uM 100uM 15,750,000 11,000,000 15,750,000 13,000,000 38,250,000 32,750,000 13,250,000 35,250,000 16,000,000 6,500,000 15,250,000 sample lost 20,250,000 37,750,000 27,500,000 13,500,000 16,000,000 24,250,000 14,250,000 21,500,000 21,750,000 15,750,000 34,500,000 26,000,000 18,750,000 26,750,000 37,500,000 32,500,000 6,750,000 5,500,000 24,750,000 11,000,000 29,500,000 28,500,000 59,500,000 19,000,000 23,750,000 36,000,000 22,250,000 11,250,000 10,500,000 36,000,000 14,000,000 15,500,000 22,000,000 12,000,000 32,500,000 27,500,000 20,000,000 36,000,000 13,500,000 12,750,000 23,500,000 17,250,000 11,750,000 15,500,000 12,500,000 13,500,000 33,500,000 24,500,000 41,750,000 9,250,000 12,250,000 21,000,000 15,250,000 11,000,000 26,750,000 12,250,000 43,750,000 20,250,000 48,250,000 17,500,000 8,750,000 35,250,000 28,750,000 30,500,000 22,250,000 11,000,000 13,500,000 22,000,000 16,250,000 22,000,000 38,750,000 9,750,000 23,500,000 22,750,000 6,750,000 27,250,000 12,250,000 23,750,000 26,000,000 5,750,000 15,250,000 20,000,000 27,500,000 16,000,000 20,500,000 21,250,000 11,750,000 29,500,000 15,250,000 8,500,000 22,500,000 7,000,000 27,250,000 9,750,000 18,000,000 14,250,000 21,000,000 11,750,000 11,500,000 12,750,000 31,000,000 17,500,000 9,000,000 9,250,000 14,250,000 40,750,000 15,750,000 20,000,000 15,500,000 13,250,000 12,500,000 31,250,000 23,500,000 14,750,000 18,000,000 24,750,000 41,750,000 14,750,000 9,750,000 31,250,000 8,500,000 24,250,000 23,750,000 26,500,000 14,000,000 19,000,000 28,500,000 21,500,000 11,500,000 22,750,000 22,250,000 15,500,000 17,250,000 14,750,000 23,250,000 12,000,000 36,000,000 28,250,000 22,000,000 36,250,000 15,250,000 25,500,000 14,000,000 26,250,000 6,500,000 7,250,000 49,750,000 24,000,000 26,750,000 30,500,000 16,000,000 9,250,000 34,750,000 13,750,000 21,000,000 12,750,000 16,000,000 30,500,000 19,250,000 40,500,000 41,500,000 24,750,000 15,750,000 21,000,000 27,000,000 11,500,000 21,000,000 24,500,000 29,500,000 8,000,000 22,750,000 20,750,000 15,750,000 8,250,000 22,250,000 34,250,000 14,500,000 16,250,000 22,500,000 27,250,000 6,750,000 22,250,000 18,000,000 27,000,000 7,250,000 23,000,000 15,500,000 16,500,000 22,000,000 38,250,000 18,000,000 22,000,000 14,750,000 19,000,000 34,250,000 36,000,000 36,250,000 15,000,000 27,000,000 17,250,000 32,000,000 25,750,000 15,250,000 22,750,000 23,250,000 17,000,000 19,500,000 27,500,000 NE susceptibility Susceptibility of G. mellonella to different concentrations of Norepinephrine- Healthy index and survival curve PBS 100pM 1nM 10nM 100nM 1uM 10uM 100uM dia 1 9 0 9 10 9 9 8 9 dia PBS 100pM 1nM 10nM 100nM 1uM 10uM 100uM 9 8 9 10 8 8 9 9 5 0 0= Alive larvae 9.5 8.5 9.5 10 8 8 8 9 5 0 1= Dead larvae 9.5 8 9.5 10 8 8 9 9.5 5 0 9.5 8 10 9 8 9.5 9 9.5 5 0 9.5 10 10 9 9 9.5 9 9.5 5 0 9.5 10 10 9 9.5 9.5 9 9.5 5 0 9.5 10 10 9 10 9.5 9.5 9.5 5 0 9.5 10 10 9 10 10 9 9.5 5 0 10 10 10 8 10 9.5 9 9.5 5 0 10 10 10 7.5 9.5 9.5 8 9.5 5 0 9 10 10 9 9.5 10 8.5 10 5 0 10 10 10 10 10 10 10 10 5 0 10 10 10 10 9.5 10 10 10 5 0 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 5 0 10 10 10 9.5 9.5 10 10 10 5 0 10 10 10 10 9.5 10 10 9 5 0 10 10 10 10 10 10 10 9 5 0 10 9 10 10 9 10 10 9 5 0 dia 2 9 0 0 0 8 9 7 0 5 0 9 9 10 8 8 9 7.5 7.5 1 1 9.5 9 10 8 8 9 7 9 5 0 9.5 9 10 9.5 8 9 9 10 5 0 9.5 9 10 10 8 9 10 10 5 0 9.5 9 10 8.5 9 9 10 10 5 0 10 9.5 10 9.5 9 9 10 9.5 5 0 10 9.5 10 9.5 10 9 10 8 5 0 10 9 10 9 9 9 10 9 5 0 10 9 10 9 8 9 10 9 5 0 10 10 10 9.5 9 9 10 9 5 0 10 10 10 10 9 9 10 9 5 0 10 10 10 10 9 9 9 9 5 0 10 10 10 10 9 9 9 9 5 0 10 10 10 9 9 9 9 9 5 0 10 10 10 9 9 9 9 9 5 0 10 10 10 9 10 8 10 9 5 0 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 9 5 0 9 10 10 10 10 10 10 9 5 0 Dia 3 9 0 0 0 8 9 7 0 5 0 9 9 10 8 8 9 7.5 7.5 2 1 9.5 9 10 8 8 9 7 9 5 0 9.5 9 10 9.5 8 9 9 10 5 0 9.5 9 10 10 8 9 10 10 5 0 9.5 9 10 8.5 9 9 10 10 5 0 10 9.5 10 9.5 9 9 10 9.5 5 0 10 9.5 10 9.5 10 9 10 8 5 0 10 9 10 9 9 9 10 9 5 0 10 9 10 9 8 9 10 9 5 0 10 10 10 9.5 9 9 10 9 5 0 10 10 10 10 9 9 10 9 5 0 10 10 10 10 9 9 9 9 5 0 10 10 10 10 9 9 9 9 5 0 10 10 10 9 9 9 9 9 5 0 10 10 10 9 9 9 9 9 5 0 10 10 10 9 10 8 10 9 5 0 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 9 5 0 9 10 10 10 10 10 10 9 5 0 dia 4 9 0 0 0 9 8 9 0 5 0 9 9 8 9 8 8 8.5 8 5 0 9 9 8.5 9 8 9 8.5 8 2 1 10 9 8.5 9 9 9 8.5 9 5 0 10 10 9.5 9 9 9 8 9 5 0 10 10 9.5 9 9 9 8 9 5 0 10 10 9 10 10 9 10 9 5 0 10 10 9 10 10 9 10 9 5 0 10 10 8.5 10 10 9 10 9 5 0 10 10 9.5 10 10 9 10 9 5 0 10 10 9.5 10 10 9 9 9 5 0 10 10 9.5 10 10 9 9 9 5 0 10 10 9 10 10 9 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 dia 5 9.5 0 0 0 9 8 8 0 5 0 9.5 9 9 8 9 8 8 8 5 0 10 8 10 8 8 8 8 8 5 0 10 8 9 9 8 8 8 8 5 0 10 8 9 10 9 9 9 8.5 5 0 10 9 9 10 9 9 9 8.5 5 0 10 9 9 10 9 9 9 10 5 0 10 9 9 9 9 9 9 9 5 0 10 9 9 9 9 9 9 9 5 0 10 9 10 9 9 9 9 9 5 0 10 9 10 10 9 9 9 9 5 0 10 9 10 9 9 9 9 9 5 0 10 10 10 9 9 9 9 9 5 0 10 10 10 9 10 10 9 9 5 0 10 10 10 9 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 10 10 10 10 10 10 9 9 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 NE hemocytes 30 min Number of circulating hemocytes 30 min after the injection of different concentrations of norepinephrine or PBS (Control) hemocytes 30 min PBS 100pM 1nM 10nM 100nM 1uM 10uM 100uM 33250000 26500000 12750000 17250000 2750000 9000000 20000000 13000000 sample lost 11750000 31000000 35000000 18000000 23250000 1000000 12250000 17750000 13750000 27000000 25500000 5000000 22000000 3000000 18750000 15250000 38000000 19250000 20250000 12750000 15750000 12500000 21500000 13000000 9500000 11000000 16500000 3250000 19500000 2500000 18000000 16750000 30750000 31250000 7000000 12000000 14500000 12000000 7500000 12000000 26000000 25750000 41500000 1750000 7750000 12500000 5500000 8000000 24000000 22000000 33750000 15250000 17250000 8750000 11250000 19750000 10250000 21500000 17750000 11250000 18250000 12750000 23250000 19500000 9750000 21500000 39750000 20750000 16750000 6250000 15500000 17500000 21000000 16750000 44000000 10500000 11750000 6500000 18000000 17500000 16500000 19750000 28000000 16000000 7000000 7500000 8500000 25000000 13750000 27750000 9500000 22000000 10000000 13250000 12500000 11500000 14750000 29750000 25250000 24500000 6250000 7250000 8000000 14250000 14000000 12250000 16250000 18750000 8500000 2500000 15750000 12750000 15750000 16500000 16000000 15000000 10250000 7000000 16250000 8500000 3500000 11000000 34000000 29250000 13250000 22750000 11000000 29250000 15250000 22000000 16250000 14750000 15750000 13000000 15750000 5500000 20750000 12000000 22750000 13500000 22250000 8000000 15000000 16750000 8500000 3000000 34000000 24000000 7750000 7000000 11250000 NE hemocytes 90 min Number of circulating hemocytes 90 min after the injection of different concentrations of norepinephrine or PBS (Control) hemocytes 90 min PBS 100pM 1nM 10nM 100nM 1uM 10uM 100uM lost sample 8000000 41500000 16500000 28750000 26500000 30500000 19250000 20000000 9250000 26500000 17000000 26750000 23750000 23500000 11750000 32250000 8250000 20750000 3500000 22500000 20750000 19750000 7250000 17750000 16250000 18500000 13500000 14500000 15750000 20750000 14750000 10000000 31750000 32750000 4250000 17250000 29500000 15750000 23750000 8000000 8500000 18250000 30250000 34750000 6750000 11500000 17750000 9250000 5750000 9000000 10500000 8500000 9500000 13000000 7000000 10750000 8250000 8500000 9000000 11000000 30500000 19750000 10750000 19750000 42750000 36750000 22000000 15250000 10750000 9000000 9750000 6250000 12000000 42750000 17750000 20750000 12250000 8250000 5000000 26500000 8000000 14250000 40750000 14250000 18250000 28250000 6000000 19500000 9750000 7250000 40250000 32000000 20750000 20750000 24500000 36750000 26000000 38750000 16500000 50750000 34000000 16750000 18750000 7500000 28500000 30500000 20750000 17500000 9750000 31500000 28000000 11750000 10500000 28500000 16250000 19750000 12500000 30000000 20250000 26750000 23750000 8000000 18000000 15500000 4000000 6000000 23000000 13750000 21500000 17000000 13250000 27250000 7500000 24500000 15500000 16000000 27500000 13500000 10500000 18250000 29000000 13000000 5000000 18000000 20750000 2750000 9250000 26500000 21250000 9250000 17500000 11500000 28750000 7750000 3500000 21250000 2750000 3750000 NE hemocytes 180 min Number of circulating hemocytes 180 min after the injection of different concentrations of norepinephrine or PBS (Control) hemocytes 180 min PBS 100pM 1nM 10nM 100nM 1uM 10uM 100uM 28750000 8750000 28750000 10250000 12000000 16000000 33500000 12500000 18500000 15000000 7250000 10500000 21250000 14250000 14000000 21250000 8750000 46250000 8500000 21500000 17000000 18750000 18250000 16000000 20500000 39500000 11250000 27250000 16250000 16000000 23000000 9250000 31250000 13500000 19250000 16250000 4250000 45750000 30000000 14000000 21250000 5750000 23250000 17750000 5750000 24250000 19250000 15000000 14250000 14000000 37500000 6000000 6500000 9500000 25500000 35250000 8750000 4500000 36000000 6250000 9000000 10750000 10750000 5500000 23500000 15500000 15500000 16250000 12500000 7500000 7500000 8500000 22500000 17750000 22250000 16000000 17500000 8250000 8250000 22500000 28000000 15000000 11250000 32000000 10500000 14500000 29500000 18750000 16250000 10750000 17000000 34500000 13500000 11500000 18250000 18500000 15000000 10500000 15750000 38000000 15750000 25750000 20000000 22750000 7250000 10750000 20750000 22750000 24000000 16000000 28500000 31000000 41750000 7500000 12000000 11500000 25750000 21750000 44500000 28000000 19250000 10250000 5250000 9500000 17750000 4750000 9000000 15500000 21750000 8750000 5500000 12500000 13000000 5500000 8000000 23250000 30500000 17250000 8000000 18000000 22000000 16250000 16750000 11250000 11500000 5750000 6250000 9500000 5250000 15500000 35500000 8250000 14750000 6250000 15000000 21250000 12500000 12500000 14250000 9500000 OCT susceptibility Susceptibility of G. mellonella to different concentrations of Octopamine- Healthy index and survival curve PBS 10pM 100pM 1nM 10nM 100nM 1uM 10uM 100uM Day 1 9 10 10 10 10 9 7 0 8 survival 10 10 10 10 10 10 7 9 8 Days PBS 10pM 100pM 1nM 10nM 100nM 1uM 10uM 100uM 0= alive 10 10 10 10 10 10 7 9 8 5 0 1- dead 10 10 10 10 10 10 7 9 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 9 10 10 10 10 9 7 0 8 5 0 10 10 10 10 10 10 7 9 8 5 0 10 10 10 10 10 10 7 9 8 5 0 10 10 10 10 10 10 7 9 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 Day 2 10 10 10 10 10 10 10 0 10 5 0 10 9 9 10 9 10 9 9 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 10 9 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 0 10 5 0 10 9 9 10 9 10 9 9 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 9 10 10 10 9 5 0 10 10 10 10 10 10 10 10 9 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 5 0 Day 3 9 9 0 9 9 0 10 0 7 3 1 9 10 10 9 10 10 10 9 9 3 1 9 10 10 10 10 10 10 9 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 9 9 0 9 9 0 10 0 7 5 0 9 10 10 9 10 10 10 9 9 5 0 9 10 10 10 10 10 10 9 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 10 10 10 10 10 10 10 10 10 5 0 5 0 Day 4 9 9 0 9 9 0 8 0 0 5 0 9 9 10 9 9.5 9 8 8 9 5 0 9 10 10 10 9.5 10 8 8 9 5 0 10 10 10 10 9.5 10 8 10 10 5 0 10 10 10 10 9.5 10 8 10 10 5 0 10 10 10 10 8.5 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 9 9 0 9 9 0 8 0 0 5 0 9 9 10 9 9.5 9 8 8 9 5 0 9 10 10 10 9.5 10 8 8 9 5 0 10 10 10 10 9.5 10 8 10 10 5 0 10 10 10 10 9.5 10 8 10 10 5 0 10 10 10 10 8.5 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 10 10 10 10 10 10 9 10 10 5 0 5 0 Day 5 10 10 0 9 9.5 0 8 0 0 5 0 10 10 10 9 9.5 10 8 9 0 5 0 10 10 10 10 9.5 10 8 8.5 8 5 0 10 10 10 10 9.5 10 8 10 9 5 0 10 10 10 10 10 10 8 10 9 5 0 10 10 10 10 10 10 8 10 9 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 0 9 9.5 0 8 0 0 5 0 10 10 10 9 9.5 10 8 9 8 5 0 10 10 10 10 9.5 10 8 8.5 8 5 0 10 10 10 10 9.5 10 8 10 9 5 0 10 10 10 10 10 10 8 10 9 5 0 10 10 10 10 10 10 8 10 9 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 5 0 10 10 10 10 10 10 8 10 10 3 1 10 10 10 10 10 10 8 10 10 3 1 5 0 Day 6 9 9 0 8 7 0 7 0 0 5 0 9 9 8 8 9 9 7 8 0 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 0 8 7 0 7 0 0 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 5 0 Day 7 9 9 0 8 7 0 7 0 0 5 0 9 9 8 8 9 9 7 8 0 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 0 8 7 0 7 0 0 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 8 9 9 7 8 9 5 0 9 9 8 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 1 1 9 9 10 9 10 9 7 8 9 1 1 9 9 10 9 10 9 7 8 9 5 0 9 9 10 9 10 9 7 8 9 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 4 1 4 1 5 1 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 OCT hemocytes 30 min Number of circulating hemocytes 30 min after the injection of different concentrations of Octopamine or PBS (Control) Octopamine 30 min PBS 10pM 100pM 1nM 10nM 100nM 1uM 10uM 100uM 17000000 11500000 17250000 25000000 7750000 25000000 10000000 11750000 7000000 17500000 13500000 21750000 24750000 33500000 19500000 11750000 4500000 14750000 13000000 5000000 31250000 36500000 16500000 2450000 10000000 11000000 14500000 9500000 11750000 34750000 18500000 21750000 13250000 19500000 10750000 17000000 21000000 16750000 28000000 27750000 18250000 35750000 28250000 22000000 12250000 16750000 16000000 11750000 21000000 22000000 25000000 19000000 23250000 23000000 13250000 13500000 16500000 25750000 34750000 15000000 21500000 17750000 16000000 11000000 13250000 29500000 42500000 19250000 19250000 2750000 10500000 27500000 12500000 16750000 25000000 16250000 18250000 15250000 5500000 8000000 7000000 14000000 12500000 19750000 25500000 16750000 9500000 17750000 5750000 6000000 27000000 13250000 28000000 19750000 18250000 18000000 4250000 12750000 16500000 12000000 12250000 36000000 19000000 9250000 13750000 5000000 20750000 21250000 9000000 6000000 34000000 22750000 19750000 3250000 15250000 8500000 18000000 11750000 26250000 26250000 26500000 12250000 2500000 9000000 8750000 12000000 23250000 15750000 27000000 15500000 22750000 8250000 15000000 8500000 8500000 19750000 29000000 31500000 32500000 31500000 7250000 12500000 17750000 26500000 20000000 24750000 15500000 22500000 36250000 10500000 7500000 18500000 14250000 17750000 26750000 28500000 26750000 9250000 31750000 9250000 12750000 12750000 26250000 13000000 15000000 38250000 8500000 26000000 12500000 18500000 13500000 31250000 8000000 26500000 28000000 9000000 34500000 8750000 15750000 14250000 100pM ISO +P. gingivalis Susceptibility of G. mellonella to the co-injection of ISO and P. gingivalis- Healthy index and survival curve Survival Healthy index Day PBS +PBS 100pM +PBS PBS + 108 P. gingivalis 108 P. gingivalis + 100pM PBS +PBS 100pM +PBS PBS + 108 P. gingivalis 108 P. gingivalis + 100pM 5 0 0= alive Day 1 9 9 0 0 5 0 1= dead 9 9 0 0 5 0 9 9 0 0 5 0 9 9.5 0 7 5 0 9.5 9.5 0 6 5 0 9.5 10 0 5 5 0 9.5 10 0 7 5 0 9.5 10 7 7 5 0 9 10 7 7 5 0 10 10 7 7 5 0 9 9 0 0 5 0 9 9 0 0 5 0 9 9 0 0 5 0 9 9.5 0 0 5 0 9 9.5 0 0 5 0 9 9.5 0 7.5 5 0 9 10 0 7.5 5 0 9 10 0 4 5 0 10 10 6 4 5 0 10 10 7.5 4 5 0 Day 2 9.5 9 0 0 5 0 9.5 9 5 6 5 0 9.5 9 5 7 5 0 9.5 9 0 6 5 0 9.5 9 0 5 5 0 10 9 0 7 5 0 10 10 0 7 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 9 9 0 0 5 0 9 9 0 0 5 0 9 9.5 0 0 5 0 9 10 0 0 5 0 9.5 10 0 0 5 0 10 10 0 7 5 0 10 10 0 8 5 0 10 10 0 8 5 0 10 10 0 0 5 0 10 10 0 0 1 1 Day 3 9 8 7.5 6 1 1 9.5 9 6.5 6 1 1 9.5 9 0 6 1 1 9.5 9.5 0 6 1 1 9.5 9.5 0 6 1 1 9.5 9.5 0 5 1 1 9.5 9.5 0 0 1 1 9.5 10 0 0 1 1 9.5 10 0 0 1 1 10 10 0 0 1 1 9 9 0 7.5 1 1 9 9 0 6.5 1 1 9 10 0 6.5 1 1 10 10 0 0 1 1 10 9.5 0 0 2 1 9.5 9.5 0 0 2 1 8.5 10 0 0 5 0 9 10 0 0 5 0 10 10 0 0 1 1 10 10 0 0 1 1 Day 4 10 9 7.5 5 1 1 10 9 6.5 6 1 1 10 9 0 6 1 1 10 9 0 6 1 1 10 9 0 6.5 1 1 10 9 0 7 1 1 10 9.5 0 0 2 1 10 10 0 0 2 1 10 9.5 0 0 2 1 10 10 0 0 5 0 9 9 0 7 5 0 9 9 0 7 5 0 9 10 0 8 5 0 9 10 0 0 5 0 9.5 9.5 0 0 5 0 9.5 9 0 0 5 0 9.5 9 0 0 5 0 10 9.5 0 0 5 0 10 9.5 0 0 10 9 0 0 Day 5 7 8 6.5 6 10 8 6.5 6 10 9 0 6 9 9.5 0 6 9 9.5 0 6.5 10 10 0 6.5 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 8 9 0 6 8 9 0 6 10 9 0 7.5 10 9 0 0 10 9 0 0 10 9 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 Hemocytes Pg +100pM ISO Number of circulating hemocytes 30 min after the injection of 100pM ISO and P. gingivalis or PBS Total hemocytes in 30 min PBS +PBS 100pM +PBS PBS + 108 P. gingivalis 108 P. gingivalis+ 100pM ISO 19000000 41750000 12750000 8250000 24500000 30750000 18750000 28000000 27250000 34750000 9500000 28250000 33750000 49750000 11250000 15000000 11750000 46000000 12750000 8750000 11750000 15250000 8250000 14500000 8750000 13500000 11500000 9750000 15500000 12250000 5000000 21500000 14250000 17500000 6500000 16750000 23500000 41500000 9750000 3750000 11000000 33000000 8750000 7250000 14750000 12750000 9000000 7250000 17000000 28500000 6250000 13250000 10500000 44250000 5500000 15750000 17000000 14000000 5250000 15750000 13250000 19000000 8750000 24000000 25750000 28500000 8500000 9250000 21000000 23000000 7500000 18000000 12250000 37750000 4750000 14250000 25250000 43250000 4250000 21500000 melanization Pg +100pM ISO Optical density (405nm) of hemolymph 30 min after the injection of 100pM ISO and P. gingivalis or PBS PBS +PBS 100pM +PBS PBS + 108 P. gingivalis 108 P. gingivalis+ 100pM ISO 0.3448333 0.1322 0.5872 0.2178333 0.5612 0.1322 0.6918333 0.2708333 0.4012 0.2218333 0.4318333 0.3688333 0.0912 0.1672 0.1522 0.3318333 0.3172 0.1322 0.3738333 0.3988333 0.2172 0.2988333 0.4328333 0.3688333 0.2388333 0.06883333 0.4358333 0.3988333 0.6382 0.2188333 0.3738333 0.2048333 0.1018333 0.1182 0.2998333 0.3668333 0.3448333 0.1182 0.5872 0.5322 0.6118333 0.3392 0.6918333 0.3668333 0.2108333 -0.00116667 0.4358333 0.1692 0.1458333 0.2658333 0.4048333 1.329833 0.1158333 0.3392 1.9832 0.5322 0.00083333 0.3692 0.4328333 0.2212 0.2388333 0.08883333 1.9832 0.2708333 0.4012 0.1972 0.1452 0.5322 0.2108333 0.2988333 1.5672 0.2048333 0.6382 0.1182 0.3918333 0.1842 0.3428333 0.1182 0.3572 0.2518333 Sessile-circulating Hem ISO Hemocyte count of sessile and circulating cells after 100pM ISO injection or PBS (Control) Collum statistics Hemocytes count- raw data PBS 100pM PBS Circulating hemocytes Sessile hemocytes Number of values 20 20 17,500,000 52,250,000 16,250,000 81,000,000 Minimum -1.873 -2.066 8,750,000 85,750,000 25% Percentile -1.165 -0.7747 17,500,000 38,000,000 Median -0.2833 -0.2158 5,500,000 38,500,000 75% Percentile 0.5454 0.1118 15,000,000 12,750,000 Maximum 3.87 1.962 18,750,000 12,250,000 26,250,000 9,750,000 Mean -0.000000014 -0.3132 6,250,000 37,250,000 Std. Deviation 1.557 1 8,250,000 14,500,000 Std. Error of Mean 0.3481 0.2236 28,500,000 28,000,000 8,000,000 21,000,000 Lower 95% CI of mean -0.7285 -0.7812 15,250,000 19,500,000 Upper 95% CI of mean 0.7285 0.1549 3,250,000 25,500,000 28,000,000 18,250,000 D'Agostino & Pearson omnibus normality test 14,500,000 35,250,000 K2 9.745 0.6185 31,000,000 12,250,000 P value 0.0077 0.734 18,750,000 23,000,000 Passed normality test (alpha=0.05)? No Yes 9,250,000 29,750,000 P value summary ** ns 25,500,000 24,500,000 100pM 32,250,000 26,000,000 Sum -0.0000002794 -6.263 20,250,000 25,000,000 Mann-whitney 63,750,000 8,750,000 Table Analyzed Hemolymph+fat body hemocytes 24,500,000 19,500,000 19,500,000 5,000,000 Column B 100pM 27,000,000 9,750,000 vs. vs. 37,250,000 8,000,000 Column A PBS 42,500,000 2,750,000 31,500,000 12,750,000 Mann Whitney test 34,750,000 3,750,000 P value 0.952 19,000,000 4,500,000 Exact or approximate P value? Exact 33,250,000 13,750,000 P value summary ns 33,500,000 9,000,000 Significantly different? (P < 0.05) No 45,000,000 17,500,000 One- or two-tailed P value? Two-tailed 16,750,000 10,500,000 Sum of ranks in column A,B 412.5 , 407.5 39,250,000 9,750,000 Mann-Whitney U 197.5 31,500,000 12,750,000 35,750,000 15,500,000 Difference between medians 9,750,000 10,500,000 Median of column A -0.2833 28,250,000 9,500,000 Median of column B -0.2158 Difference: Actual 0.06745 Difference: Hodges-Lehmann -0.04818 Sessile-circulating Hem OCT Hemocyte count of sessile and circulating cells after 100pM OCT injection or PBS (Control) Circulating hemocytes Sessile hemocytes PBS 100pM OCT PBS 100pM 11000000 20750000 45250000 9000000 22750000 13750000 24250000 7250000 11250000 20250000 32500000 12750000 28750000 47250000 24500000 18500000 27250000 39000000 26000000 18250000 18500000 18000000 18500000 8000000 16250000 51500000 23750000 16000000 9500000 39500000 21250000 6500000 3000000 45250000 29250000 8750000 15500000 33750000 23750000 22750000 17500000 27500000 19500000 7250000 10000000 11750000 33750000 17750000 20500000 21000000 28250000 7250000 29750000 35500000 32750000 13750000 13500000 31250000 22750000 11250000 15500000 30000000 26750000 9000000 23000000 32000000 340000000 12000000 18250000 38000000 28750000 11250000 20000000 34750000 25500000 7250000 14250000 41500000 29250000 11250000 hemocytes +propanolol Effect of propornaolol administration on sessile and circulating hemocytes numbers after OCT or ISO injection Circulating hemocytes PBS +PBS 100pM OCT + PBS 1nM OCT + PBS 100pM ISO +PBS 100pM OCT+ Propanolol 1nM OCT +Propanolol 100pM ISO +Propanolol Propanolol +PBS 9500000 51500000 44000000 41000000 17250000 35750000 27750000 13250000 3000000 23250000 32000000 35000000 11250000 27500000 15000000 14250000 8000000 39500000 31000000 30500000 14000000 33750000 17750000 11000000 15500000 45250000 46000000 40250000 9250000 21000000 15500000 15000000 11000000 33750000 18250000 40250000 12250000 11250000 14750000 21500000 17500000 27500000 22500000 13750000 36250000 13750000 17250000 21750000 32250000 13500000 26000000 30500000 8500000 11500000 31000000 14000000 10000000 11750000 20250000 15250000 8000000 15000000 13750000 12750000 20500000 10500000 8250000 16250000 8250000 6750000 13750000 21500000 29750000 21000000 16250000 29250000 7250000 8000000 12750000 24750000 30250000 23250000 31500000 17750000 13000000 24250000 13250000 8750000 13500000 35500000 30250000 31250000 24000000 18000000 15000000 15250000 15500000 31250000 23750000 31750000 25500000 10500000 20750000 29500000 23000000 30000000 18750000 33750000 16500000 3750000 17500000 29750000 24750000 32000000 32750000 37750000 12750000 16250000 33250000 23750000 18250000 30000000 39000000 26500000 23500000 14000000 24750000 23750000 17500000 38000000 38000000 26250000 8000000 23000000 17500000 12500000 20000000 34750000 52000000 42250000 11500000 18000000 17000000 33250000 23000000 29750000 27750000 23250000 14750000 16000000 25000000 23750000 14250000 41500000 30500000 22500000 15500000 23500000 22250000 25500000 Sessile hemocytes PBS +PBS 100pM OCT + PBS 1nM OCT + PBS 100pM ISO +PBS 100pM OCT+ Propanolol 1nM OCT +Propanolol 100pM ISO +Propanolol Propanolol +PBS 21250000 16000000 9750000 20000000 21750000 12250000 13500000 16500000 29250000 19250000 12500000 11250000 15500000 22000000 34750000 13250000 28500000 6500000 11250000 11750000 18000000 11750000 25000000 30250000 23750000 8750000 22750000 13750000 26000000 30750000 21250000 45250000 35750000 22750000 15500000 23500000 20250000 33250000 31250000 48750000 19500000 7250000 14500000 17750000 10500000 28750000 21000000 49250000 22250000 9750000 10500000 17500000 44750000 26750000 17250000 14000000 33750000 17750000 8500000 21250000 38000000 22750000 15500000 26250000 28250000 16000000 9000000 5250000 39000000 23750000 26500000 35250000 32750000 7250000 15750000 17000000 45000000 30000000 26250000 21750000 14500000 14250000 11000000 10250000 35750000 24500000 18250000 26250000 22750000 13750000 16250000 10250000 21500000 27000000 14250000 24250000 26750000 11250000 13750000 4250000 12250000 27250000 17500000 17000000 340000000 9000000 27500000 5500000 37500000 36500000 18750000 14250000 15750000 12000000 15500000 5000000 37250000 14750000 12000000 20500000 28750000 12000000 9750000 7250000 13750000 42750000 20000000 21750000 16750000 11250000 9500000 7750000 33250000 15750000 17000000 32750000 25500000 7250000 11500000 7500000 38500000 33250000 16250000 18750000 22250000 15000000 17750000 12250000 32750000 15750000 13750000 22000000 29250000 11250000 11750000 7250000 40000000 17250000 17750000 15000000 NE +P.gingivalis Susceptibility of G. mellonella to the co-injection of NE and P. gingivalis- Healthy index, survival curve and hemocyte count Healthy index survival Hemocytes PBS +PBS 1uM NE + PBS 108 P. gingivalis + PBS P. gingivalis +1uM NE days PBS +PBS 1uM NE + PBS 108 P. gingivalis + PBS P. gingivalis +1uM NE PBS +PBS 1uM NE + PBS 108 P. gingivalis + PBS P. gingivalis +1uM NE dia 1 9 8 0 0 5 0 0= alive 16750000 13250000 2750000 5250000 10 8 0 0 5 0 1= dead 17750000 4250000 6000000 4250000 10 8 0 0 5 0 16500000 5250000 4000000 3500000 10 9 0 0 5 0 22000000 13250000 3000000 1500000 10 9 0 0 5 0 12500000 20000000 3500000 2750000 10 9 0 0 5 0 14500000 13500000 2250000 4250000 10 9 5 0 5 0 13750000 14250000 7750000 3000000 10 9 6 0 5 0 17250000 23000000 3750000 8500000 10 9 6 0 5 0 13500000 18000000 5500000 16500000 10 9 7 3 5 0 32250000 10000000 11000000 7750000 9 9 0 0 5 0 28750000 8250000 13750000 7750000 10 10 0 0 5 0 3350000 6250000 11250000 1500000 10 10 0 0 5 0 18000000 15250000 13250000 1750000 10 10 0 0 5 0 33000000 21500000 9250000 16500000 10 10 0 0 5 0 24750000 9500000 2750000 2500000 10 10 0 0 5 0 27500000 9250000 2250000 750000 10 10 0 0 5 0 16750000 18500000 5000000 1250000 10 10 8 0 5 0 26250000 15250000 7250000 2000000 10 10 6 0 5 0 23500000 23500000 8250000 4000000 10 10 3 0 5 0 16750000 18500000 2000000 3000000 dia 2 10 8 0 0 5 0 10 8 0 0 5 0 10 8 0 0 5 0 10 9.5 0 0 5 0 10 9 0 0 5 0 10 9 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 7 0 5 0 10 10 4 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 0 0 5 0 10 10 5 0 5 0 10 10 5 0 5 0 dia 3 9 8.5 0 0 1 1 9 8.5 0 0 1 1 9 8.5 0 0 1 1 9 9 0 0 1 1 10 9 0 0 1 1 10 9 0 0 1 1 10 9 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 6 0 1 1 9 9 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 2 1 10 10 0 0 2 1 10 10 0 0 2 1 10 10 0 0 3 1 10 10 0 0 3 1 10 10 0 0 5 1 10 10 5 0 5 0 dia 4 9 8.5 0 0 1 1 9 8.5 0 0 1 1 9 8.5 0 0 1 1 9 9 0 0 1 1 10 9 0 0 1 1 10 9 0 0 1 1 10 9 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 7 0 1 1 9 9 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 0 0 1 1 10 10 5 0 2 1 dia 5 9 8 0 0 9 8 0 0 9 8 0 0 8 9 0 0 8 9 0 0 10 9 0 0 10 9 0 0 10 10 0 0 10 10 0 0 10 10 0 0 9 9 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 5 0 Ne+ Pg melanization Optical density (405nm) of hemolymph 30 min after the injection of 1uM NE and P. gingivalis or PBS OD 405nm PBS +PBS 1uM NE +PBS 108 P. gingivalis +PBS 108 P.gingivalis+1uM NE lost sample 0.216 0.2 0.781 0.187 0.3448333 0.143 0.305 0.3738333 0.142 0.187 0.341 0.478 0.2172 0.125 0.219 0.444 0.172 0.642 0.5872 0.4358333 0.193 0.175 0.932 0.531 0.075 0.239 1.29 0.329 0.025 1.053 1.007 0.347 0.1158333 0.17 0.6918333 0.235 0.543 0.553 0.16 0.00083333 0.168 0.4318333 0.611 0.1018333 0.203 0.3572 0.269 0.172 0.711 0.6918333 0.406 -0.01 0.109 0.424 0.3738333 0.09 0.2 0.686 1.358 0.271 0.223 0.655 1.5672 0.266 0.472 0.274 0.078 0.316 0.634 0.486 0.001 0.517 0.55 0.582 0.097 0.895 1.9832 Pg recovery hemolymph Number of colonies forming units in the hemolymph of G. mellonella larvae 30 min after injection. CFU/mL 108 CFU/mL P.gingivalis +PBS 108 CFU/mL P.gingivalis + 100pM ISO 108 CFU/mL P.gingivalis + 1µM NE 26700000 23800000 21200000 29800000 4400000 20400000 20400000 22300000 32800000 22600000 25000000 8600000 10400000 6200000 15100000 22800000 2100000 9100000 39600000 7200000 13100000 29200000 14800000 10300000 9000000 11000000 16400000 7800000 4400000 6300000 nodules formation Number of nodules on hystological sections P. gingivalis 108CFU/mL + PBS P. gingivalis 108CFU/mL +100pM ISO P. gingivalis 108CFU/mL +1uM NE transversal transversal half 1 half 2 half 1 half 2 half 1 half 2 PGNE7 1 19 24 PG 1 1 32 33 PGISO 6 1 15 11 2 22 28 2 33 33 2 14 20 3 17 24 3 33 20 3 15 16 PGNE14 1 23 29 PG2 1 8 7 PGISO 14 1 15 22 2 14 21 2 9 19 2 14 18 3 14 20 3 9 19 3 17 20 PGNE1 1 19 20 PG15 1 20 25 PGISO16 1 14 14 2 18 21 2 23 28 2 10 11 3 18 24 3 16 24 3 15 14 PGNE 1 15 20 PG8 1 23 24 PGISO15 1 12 18 2 15 19 2 22 24 2 12 16 3 16 18 3 18 25 3 11 9 PGNE10 1 18 15 PG20 1 25 23 PGISO3 1 9 22 2 19 14 2 35 24 2 11 19 3 19 16 3 25 30 3 12 17 PGNE18 1 12 20 PG13 1 23 31 PGISO11 1 8 9 2 17 15 2 25 22 2 4 10 3 16 25 3 28 17 3 9 11 PGNE13 1 23 28 PG19 1 18 16 PGISO13 1 14 14 2 21 33 2 16 19 2 15 15 3 27 21 3 16 12 3 16 16 PGNE12 1 7 6 PG18 1 22 17 PGISO10 1 13 16 2 9 12 2 16 21 2 22 15 3 5 18 3 16 23 3 15 16 PGNE4 1 13 14 PG14 1 21 16 PGISO1 1 10 21 2 7 16 2 28 29 2 13 21 3 25 10 3 27 10 3 15 25 PGNE19 1 6 18 PGISO 4 1 15 15 2 9 12 2 19 14 3 7 11 3 15 16 PGISO 2 1 9 14 2 8 9 3 10 12 AMPs ef-1a gloverin ef-1a cecropin CT1 CT2 mean CT1 CT2 mean CT1 CT2 mean CT1 CT2 mean PBS1 20.6022319794 18.6908493042 19.6465406418 PBS1 29.0215549469 28.6207275391 28.821141243 PBS1 20.6022319794 18.6908493042 19.6465406418 PBS1 24.0209751129 24.5910396576 24.3060073853 PBS3 16.3365592957 16.334695816 16.3356275558 PBS3 24.965057373 25.1456508636 25.0553541183 PBS3 16.3365592957 16.334695816 16.3356275558 PBS3 21.4471435547 21.5767784119 21.5119609833 PBS4 21.9389724731 21.8830795288 21.911026001 PBS4 31.2023029327 31.7333927155 31.4678478241 PBS4 21.9389724731 21.8830795288 21.911026001 PBS4 26.9583415985 26.7368373871 26.8475894928 PBS5 19.8707675934 19.6872692108 19.7790184021 PBS5 26.4860610962 25.9344158173 26.2102384567 PBS5 19.8707675934 19.6872692108 19.7790184021 PBS5 25.2359218597 24.9431476593 25.0895347595 PBS1_1 16.8620948792 16.6889533997 16.7755241394 PBS1_1 28.430305481 28.3562583923 28.3932819366 PBS1_1 16.8620948792 16.6889533997 16.7755241394 PBS1_1 22.9611263275 23.4079341888 23.1845302582 PBS3_1 17.0363845825 16.9968070984 17.0165958405 PBS3_1 27.0236682892 26.9567718506 26.9902200699 PBS3_1 17.0363845825 16.9968070984 17.0165958405 PBS3_1 22.302822113 22.2166843414 22.2597532272 PBS6 19.1667690277 18.7743282318 18.9705486298 PBS6 28.8532867432 28.9581222534 28.9057044983 PBS6 19.1667690277 18.7743282318 18.9705486298 PBS6 24.5706882477 22.9613552094 23.7660217285 PBS7 Undetermined 18.4874897003 18.4874897003 PBS7 28.9293746948 27.6332111359 28.2812929153 PBS7 Undetermined 18.4874897003 18.4874897003 PBS7 23.5147953033 23.3258113861 23.4203033447 PBS8 16.6485061646 17.4252281189 17.0368671417 PBS8 24.2792987823 24.7927131653 24.5360059738 PBS8 16.6485061646 17.4252281189 17.0368671417 PBS8 22.5524749756 21.3345088959 21.9434919357 PBS9 17.2095584869 18.2367801666 17.7231693268 PBS9 25.3612442017 27.3185596466 26.3399019241 PBS9 17.2095584869 18.2367801666 17.7231693268 PBS9 23.2727222443 23.371301651 23.3220119476 PBS10 19.2405643463 18.6249256134 18.9327449799 PBS10 28.5151042938 26.7820301056 27.6485671997 PBS10 19.2405643463 18.6249256134 18.9327449799 PBS10 23.5163135529 24.0808467865 23.7985801697 PG1 17.8885269165 17.8634090424 17.8759679794 PG1 21.6219959259 20.9890842438 21.3055400848 PG1 17.8885269165 17.8634090424 17.8759679794 PG1 18.7658939362 18.3202552795 18.5430746078 PG3 20.7569084167 20.7417926788 20.7493505478 PG3 28.0954189301 27.7842597961 27.9398393631 PG3 20.7569084167 20.7417926788 20.7493505478 PG3 24.8908119202 24.6298961639 24.7603540421 PG4 17.2379684448 17.0773124695 17.1576404572 PG4 24.9044475555 24.2407779694 24.5726127625 PG4 17.2379684448 17.0773124695 17.1576404572 PG4 20.2374916077 20.2505016327 20.2439966202 PG5 16.736989975 17.4413375854 17.0891637802 PG5 21.9090118408 22.1667728424 22.0378923416 PG5 16.736989975 17.4413375854 17.0891637802 PG5 20.4437160492 20.4852485657 20.4644823074 PG6 16.9437656403 16.8941497803 16.9189577103 PG6 20.4524784088 20.4313850403 20.4419317245 PG6 16.9437656403 16.8941497803 16.9189577103 PG6 18.818403244 18.7448730469 18.7816381454 PG7 16.6938209534 16.8890857697 16.7914533615 PG7 20.2936897278 20.0723972321 20.1830434799 PG7 16.6938209534 16.8890857697 16.7914533615 PG7 18.2354698181 18.1405105591 18.1879901886 PG8 20.8524112701 18.0086688995 19.4305400848 PG8 22.7923030853 20.627822876 21.7100629807 PG8 20.8524112701 18.0086688995 19.4305400848 PG8 20.8878154755 21.1387405396 21.0132780075 PG9 17.2661247253 16.5598392487 16.912981987 PG9 20.2662601471 19.6971912384 19.9817256927 PG9 17.2661247253 16.5598392487 16.912981987 PG9 19.6705474854 19.4556179047 19.563082695 PG10 17.8739242554 18.60247612 18.2382001877 PG10 22.0839195251 20.7569141388 21.420416832 PG10 17.8739242554 18.60247612 18.2382001877 PG10 22.4921474457 20.7718524933 21.6319999695 PG11 17.9465999603 17.9028301239 17.9247150421 PG11 19.353012085 19.5057086945 19.4293603897 PG11 17.9465999603 17.9028301239 17.9247150421 PG11 20.3652095795 20.397687912 20.3814487457 PG12 26.1346950531 26.3067245483 26.2207098007 PG12 29.5532569885 29.3903064728 29.4717817307 PG12 26.1346950531 26.3067245483 26.2207098007 PG12 30.5960636139 30.823223114 30.709643364 PG13 26.1505756378 26.100894928 26.1257352829 PG13 29.1423339844 29.2580909729 29.2002124786 PG13 26.1505756378 26.100894928 26.1257352829 PG13 30.4713134766 30.5365829468 30.5039482117 PGISO1 16.1895446777 16.3449859619 16.2672653198 PGISO1 19.9412059784 19.9581336975 19.949669838 PGISO1 16.1895446777 16.3449859619 16.2672653198 PGISO1 21.6894702911 21.6057319641 21.6476011276 PGISO2 16.7815170288 16.5900344849 16.6857757568 PGISO2 18.6597213745 18.6317024231 18.6457118988 PGISO2 16.7815170288 16.5900344849 16.6857757568 PGISO2 20.2531375885 20.0347576141 20.1439476013 PGISO3 16.178571701 16.0142173767 16.0963945389 PGISO3 20.1127758026 20.0121860504 20.0624809265 PGISO3 16.178571701 16.0142173767 16.0963945389 PGISO3 23.1418933868 22.7316970825 22.9367952347 PGISO4 17.699672699 17.6106796265 17.6551761627 PGISO4 19.6000213623 19.4393577576 19.5196895599 PGISO4 17.699672699 17.6106796265 17.6551761627 PGISO4 20.879201889 20.8766708374 20.8779363632 PGISO5 16.6272716522 16.5339107513 16.5805912018 PGISO5 18.2061653137 18.1357383728 18.1709518433 PGISO5 16.6272716522 16.5339107513 16.5805912018 PGISO5 19.9724063873 19.7172279358 19.8448171616 PGISO6 17.3498077393 17.4041538239 17.3769807816 PGISO6 18.9800167084 19.0655841827 19.0228004456 PGISO6 17.3498077393 17.4041538239 17.3769807816 PGISO6 20.8675231934 20.6299819946 20.748752594 PGISO6 13.7643623352 12.7194604874 13.2419114113 PGISO6 20.0192966461 19.3067188263 19.6630077362 PGISO6 13.7643623352 12.7194604874 13.2419114113 PGISO6 20.7047157288 21.8470668793 21.275891304 PGISO7 28.2497119904 27.4224472046 27.8360795975 PGISO7 34.7307510376 33.2966423035 34.0136966705 PGISO7 28.2497119904 27.4224472046 27.8360795975 PGISO7 33.586517334 34.9466056824 34.2665615082 PGISO8 16.3713512421 17.0241889954 16.6977701187 PGISO8 25.2747802734 23.7339134216 24.5043468475 PGISO8 16.3713512421 17.0241889954 16.6977701187 PGISO8 25.4771823883 26.1973590851 25.8372707367 PGISO9 14.2997055054 14.4406919479 14.3701987267 PGISO9 20.8521347046 20.9608097076 20.9064722061 PGISO9 14.2997055054 14.4406919479 14.3701987267 PGISO9 23.1753921509 21.8237838745 22.4995880127 PGISO10 14.9291782379 12.0197095871 13.4744439125 PGISO10 18.5404682159 19.2115573883 18.8760128021 PGISO10 14.9291782379 12.0197095871 13.4744439125 PGISO10 20.944519043 20.5526981354 20.7486085892 PG+ISO 12 22.9483623505 22.9767513275 22.962556839 PG+ISO 12 29.2932949066 29.5517940521 29.4225444794 PG+ISO 12 22.9483623505 22.9767513275 22.962556839 PG+ISO 12 30.7135162354 30.3952941895 30.5544052124 PG+ISO 13 23.0354537964 23.1600627899 23.0977582932 PG+ISO 13 26.5879383087 26.5875244141 26.5877313614 PG+ISO 13 23.0354537964 23.1600627899 23.0977582932 PG+ISO 13 26.9529018402 27.0073947906 26.9801483154 PG+ISO 14 17.2037372589 17.3961849213 17.2999610901 PG+ISO 14 21.251127243 21.0969352722 21.1740312576 PG+ISO 14 17.2037372589 17.3961849213 17.2999610901 PG+ISO 14 20.4351634979 20.4206504822 20.4279069901 PGNE1 17.357624054 17.0510482788 17.2043361664 PGNE1 20.0875358582 20.0678253174 20.0776805878 PGNE1 17.357624054 17.0510482788 17.2043361664 PGNE1 18.093996048 18.277589798 18.185792923 PGNE2 19.7962665558 19.7962665558 PGNE2 18.8478393555 18.967918396 18.9078788757 PGNE2 19.7962665558 19.7962665558 PGNE2 17.5125274658 18.1683101654 17.8404188156 PGNE3 16.5845546722 16.5823955536 16.5834751129 PGNE3 25.5642108917 25.3917140961 25.4779624939 PGNE3 16.5845546722 16.5823955536 16.5834751129 PGNE3 23.1118736267 23.201379776 23.1566267014 PGNE4 17.4503440857 17.3906211853 17.4204826355 PGNE4 20.3027172089 20.1498184204 20.2262678146 PGNE4 17.4503440857 17.3906211853 17.4204826355 PGNE4 18.8125343323 18.7454662323 18.7790002823 PGNE6 14.6893911362 13.3573818207 14.0233864784 PGNE6 21.2150268555 22.1444416046 21.67973423 PGNE6 14.6893911362 13.3573818207 14.0233864784 PGNE6 18.4762744904 20.0633125305 19.2697935104 PGNE7 14.8842029572 14.678396225 14.7812995911 PGNE7 23.1734409332 23.4035606384 23.2885007858 PGNE7 14.8842029572 14.678396225 14.7812995911 PGNE7 19.4014644623 18.6539211273 19.0276927948 PGNE8 14.5013580322 14.2236871719 14.3625226021 PGNE8 20.1366539001 22.6839237213 21.4102888107 PGNE8 14.5013580322 14.2236871719 14.3625226021 PGNE8 20.8922386169 21.3173294067 21.1047840118 PGNE9 12.5554800034 13.4228239059 12.9891519547 PGNE9 20.5704135895 21.6667556763 21.1185846329 PGNE9 12.5554800034 13.4228239059 12.9891519547 PGNE9 18.8783187866 17.4137592316 18.1460390091 PGNE10 13.4041004181 13.0236949921 13.2138977051 PGNE10 20.4705924988 20.4618644714 20.4662284851 PGNE10 13.4041004181 13.0236949921 13.2138977051 PGNE10 19.6711654663 17.9706993103 18.8209323883 PG+NE 11 22.4105453491 22.4642791748 22.437412262 PG+NE 11 26.2321414948 26.3973121643 26.3147268295 PG+NE 11 22.4105453491 22.4642791748 22.437412262 PG+NE 11 27.8705749512 27.9151611328 27.892868042 PG+NE 12 17.3680667877 17.4958877563 17.431977272 PG+NE 12 21.8931846619 21.6927776337 21.7929811478 PG+NE 12 17.3680667877 17.4958877563 17.431977272 PG+NE 12 19.814907074 19.8048019409 19.8098545074 PG+NE 13 17.4596729279 17.6824989319 17.5710859299 PG+NE 13 19.9876079559 19.9917507172 19.9896793365 PG+NE 13 17.4596729279 17.6824989319 17.5710859299 PG+NE 13 20.7577438354 20.7761974335 20.7669706345 PG+NE 14 16.8464756012 16.9019241333 16.8741998672 PG+NE 14 19.9667930603 20.0379581451 20.0023756027 PG+NE 14 16.8464756012 16.9019241333 16.8741998672 PG+NE 14 20.5480422974 20.5272674561 20.5376548767 ligand action on PG Susceptibility of G. mellonella to P. gingivalis grown in the presence of NE Susceptibility of G. mellonella to P. gingivalis grown in the presence of ISO Hours PBS 109 CFU/mL P. gingivalis 109 CFU/mL PgNOR 100uM 109 CFU/mL PgNOR 1uM 0= Alive Healthy index PgNOR Hours PBS + PBS 109 CFU/mLP. Gingivalis +PBS 109 CFU/mL PgISO 100µM + PBS 109CFU/mL PgISO 100pM +PBS 109 CFU/mL PgISO 100pM +100pM ISO 0= Alive Healthy index PgISO 24 0 1= Dead larvae PBS 109 CFU/mL P. gingivalis 109 CFU/mL PgNOR 100uM 109 CFU/mL PgNOR 1uM 24 0 1= Dead larvae PBS 109 CFU/mL P. gingivalis 109 CFU/mL PgISO 100µM 109 CFU/mL PgISO 100pM 109 CFU/mL PgISO 100pM +100pM ISO 24 0 24 horas 10 0 0 0 24 0 24 horas 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 24 0 10 0 0 0 24 0 10 0 0 0 0 1 1 10 0 0 0 1 1 10 0 0 0 0 1 1 0 0 0 1 1 0 0 0 0 1 1 0 0 0 1 1 0 0 0 0 5 1 3 0 0 5 1 3 0 0 0 5 1 4.5 0 3 5 1 4.5 0 0 0 5 1 4 4 3 5 1 4 0 0 0 5 1 4 0 0 5 1 4 0 0 0 6 1 4 0 0 6 1 4 0 0 0 6 1 4 0 0 6 1 4 0 0 0 7 1 4 0 0 7 1 4 0 0 4 7 1 4 0 0 7 1 4 0 0 4 8 1 10 0 8 1 10 0 0 0 24 1 10 0 24 1 10 0 0 0 24 1 10 0 24 1 10 0 0 0 24 1 10 0 24 1 10 0 0 0 1 1 10 0 1 1 10 0 0 0 1 1 10 0 1 1 10 0 0 0 1 1 10 0 1 1 10 0 0 0 4 1 10 0 4 1 10 0 0 0 24 1 10 0 24 1 10 0 0 0 24 1 10 4.5 24 1 10 4.5 0 0 24 1 4 24 1 4 0 0 24 1 4 24 1 4 0 0 24 1 4 24 1 4 0 0 24 1 4 24 1 4 0 0 24 1 4 24 1 4 0 0 24 1 0 24 1 0 0 0 24 0 0 24 0 0 0 0 24 0 0 24 0 0 0 0 24 0 0 24 0 0 0 0 24 0 0 24 0 0 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 24 0 0 1 1 0 1 1 0 1 1 0 1 1 0 3 1 0 3 1 0 5 1 0 5 1 0 5 1 0 5 1 0 5 1 0 5 1 0 6 1 0 6 1 0 7 1 0 7 1 0 7 1 0 7 1 0 8 1 0 8 1 0 8 1 0 8 1 0 8 1 0 8 1 0 8 1 0 8 1 0 24 1 4 24 1 4 24 1 4 24 1 4 24 1 24 1 24 1 24 1 24 1 24 1 24 0 24 0 24 0 24 0 1 1 1 1 1 1 1 1 2 1 1 1 2 1 1 1 5 1 1 1 5 1 2 1 5 1 2 1 5 1 4 1 6 1 4 1 7 1 4 1 7 1 4 1 8 1 5 1 24 1 6 1 24 1 7 1 24 1 24 1 24 1 24 1 24 1 24 1 24 0 24 1 24 0 24 1 24 0 24 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 3 1 2 1 3 1 4 1 5 1 4 1 5 1 4 1 7 1 4 1 8 1 8 1 24 1 24 1 24 1 24 1 24 1 24 1 24 1 24 1 24 1 24 1 24 0 24 1 24 0 24 1 24 0 24 1 24 0 24 0 24 0 24 0 1 1 1 1 1 1 1 1 3 1 4 1 5 1 6 1 6 1 6 1 7 1 7 1 7 1 7 1 7 1 8 1 8 1 24 1 24 1 24 1 1 1 3 1 5 1 5 1 6 1 8 1 8 1 8 1 8 1 8 1 8 1 8 1 24 1 24 1 24 1 24 0 24 0 24 0 24 0 24 0 Virulence gene expression 16S rgbp 16S FimA CT1 CT2 average CT1 CT2 average CT1 CT2 average CT1 CT2 average PG2 10.5606918335 10.2551698685 10.407930851 PG2 25.3388614655 25.4582481384 25.3985548019 PG2 10.5606918335 10.2551698685 10.407930851 PG2 26.3294582367 26.7206077576 26.5250329971 PG3 9.1937379837 9.6171207428 9.4054293633 PG3 24.8348579407 24.8956031799 24.8652305603 PG3 9.1937379837 9.6171207428 9.4054293633 PG3 26.6410903931 26.5895252228 26.6153078079 PG4 9.771194458 9.2838230133 9.5275087357 PG4 23.7927970886 23.5386257172 23.6657114029 PG4 9.771194458 9.2838230133 9.5275087357 PG4 25.9307479858 25.7142181396 25.8224830627 PG5 10.2547397614 10.2440757751 10.2494077682 PG5 24.8974685669 24.0309391022 24.4642038345 PG5 10.2547397614 10.2440757751 10.2494077682 PG5 26.4014415741 26.2820091248 26.3417253494 PG6 12.1903495789 12.1247749329 12.1575622559 PG6 26.6536865234 26.7881450653 26.7209157944 PG6 12.1903495789 12.1247749329 12.1575622559 PG6 28.4335002899 28.7713470459 28.6024236679 PG7 11.9189682007 12.0145339966 11.9667510986 PG7 25.8894519806 25.5417633057 25.7156076431 pg7 11.9189682007 12.0145339966 11.9667510986 PG7 28.6383934021 28.1930427551 28.4157180786 PgNOR 100um 3 9.8521327972 9.8521327972 9.8521327972 PgNOR 100um 3 19.0704097748 20.9602603912 20.015335083 PgNOR 100um 3 9.8521327972 9.8521327972 9.8521327972 PgNOR 100um 3 22.1417350769 22.3057594299 22.2237472534 PgNOR 100um 4 8.6097631454 8.7654361725 8.687599659 PgNOR 100um 4 19.5078792572 19.412147522 19.4600133896 PgNOR 100um 4 8.6097631454 8.7654361725 8.687599659 PgNOR 100um 4 21.5738735199 21.5515975952 21.5627355576 PgNOR 100uM 6 9.2269859314 9.0205507278 9.1237683296 PgNOR 100uM 6 18.8648490906 19.7971076965 19.3309783936 PgNOR 100uM 6 9.2269859314 9.0205507278 9.1237683296 PgNOR 100uM 6 21.2093639374 21.7344646454 21.4719142914 PgNOR 100uM 7 10.2341 9.876532 10.055316 PgNOR 100uM 7 21.0982780457 20.0614566803 20.579867363 PgNOR 100uM 7 10.2341 9.876532 10.055316 PgNOR 100uM 7 22.317111969 22.3368701935 22.3269910812 PgNOR 100uM 8 9.9653787613 9.4055948257 9.6854867935 PgNOR 100uM 8 20.841753006 19.9348545074 20.3883037567 PgNOR 100uM 8 9.9653787613 9.4055948257 9.6854867935 PgNOR 100uM 8 22.0185928345 22.2842082977 22.1514005661 PgNOR 100uM 9 10.2365379333 9.2181196213 9.7273287773 PgNOR 100uM 9 20.1009044647 19.9660129547 20.0334587097 PgNOR 100uM 9 10.2365379333 9.2181196213 9.7273287773 PgNOR 100uM 9 20.9045448303 22.0440921783 21.4743185043 PgNOR 1uM 3 8.9374370575 9.0871534348 9.0122952461 PgNOR 1uM 3 19.3978652954 19.8797111511 19.6387882233 PgNOR 1uM 3 8.9374370575 9.0871534348 9.0122952461 PgNOR 1uM 3 20.932264328 21.0075187683 20.9698915482 PgNOR 1uM 4 8.2236204147 8.1324644089 8.1780424118 PgNOR 1uM 4 19.1591262817 18.8231010437 18.9911136627 PgNOR 1uM 4 8.2236204147 8.1324644089 8.1780424118 PgNOR 1uM 4 21.1115150452 21.3698616028 21.240688324 PgNOR 1uM 5 7.0790491104 7.2612695694 7.1701593399 PgNOR 1uM 5 17.1996173859 19.132900238 18.166258812 PgNOR 1uM 5 7.0790491104 7.2612695694 7.1701593399 PgNOR 1uM 5 21.5298023224 19.4803028107 20.5050525665 PgNOR 1uM 6 8.6000490189 7.2594542503 7.9297516346 PgNOR 1uM 6 19.0908164978 19.0897350311 19.0902757645 PgNOR 1uM 6 8.6000490189 7.2594542503 7.9297516346 PgNOR 1uM 6 21.0593700409 20.744638443 20.9020042419 PgNOR 1uM 7 9.3244466782 9.3396511078 9.332048893 PgNOR 1uM 7 20.7018928528 19.2981758118 20.0000343323 PgNOR 1uM 7 9.3244466782 9.3396511078 9.332048893 PgNOR 1uM 7 21.3806018829 22.7813835144 22.0809926987 PgNOR 1uM 8 9.7995090485 9.3855018616 9.592505455 PgNOR 1uM 8 20.0960712433 20.0033779144 20.0497245789 PgNOR 1uM 8 9.7995090485 9.3855018616 9.592505455 PgNOR 1uM 8 21.629945755 21.6763439178 21.6531448364 PgISO100um 3 9.202791214 10.5992212296 9.9010062218 PgISO100um 3 20.5303516388 21.1565589905 20.8434553146 PgISO100um 3 9.202791214 10.5992212296 9.9010062218 PgISO100um 3 21.6819477081 21.7505168915 21.7162322998 PgISO100um 4 9.1503095627 9.1300382614 9.140173912 PgISO100um 4 19.3610076904 19.7635002136 19.562253952 PgISO100um 4 9.1503095627 9.1300382614 9.140173912 PgISO100um 4 23.2199268341 23.7919139862 23.5059204102 PgISO100um 5 9.160399437 9.7818717957 9.4711356163 PgISO100um 5 20.7320766449 19.0204582214 19.8762674332 PgISO100um 5 9.160399437 9.7818717957 9.4711356163 PgISO100um 5 22.8599853516 22.8335914612 22.8467884064 PgISO100um 6 10.5097751617 10.4465742111 10.4781746864 PgISO100um 6 21.2809867859 20.4408245087 20.8609056473 PgISO100um 6 10.5097751617 10.4465742111 10.4781746864 PgISO100um 6 23.9500637054 23.5359897614 23.7430267334 PgISO100um 7 10.2966918945 10.5461616516 10.4214267731 PgISO100um 7 21.5615520477 21.2715549469 21.4165534973 PgISO100um 7 10.2966918945 10.5461616516 10.4214267731 PgISO100um 7 22.305727005 23.6388587952 22.9722929001 PgISO100um 8 10.9541358948 10.9590625763 10.9565992355 PgISO100um 8 22.2700920105 21.307718277 21.7889051437 PgISO100um 8 10.9541358948 10.9590625763 10.9565992355 PgISO100um 8 22.4994678497 21.7352180481 22.1173429489 PgISO 100pM 3 9.9180011749 9.5029296875 9.7104654312 PgISO 100pM 3 20.3370857239 20.0881462097 20.2126159668 PgISO 100pM 3 9.9180011749 9.5029296875 9.7104654312 PgISO 100pM 3 22.4391126633 22.2513065338 22.3452095985 PgISO 100pM 4 9.2956323624 9.3150444031 9.3053383827 PgISO 100pM 4 21.0911560059 20.0381526947 20.5646543503 PgISO 100pM 4 9.2956323624 9.3150444031 9.3053383827 PgISO 100pM 4 22.7850837708 22.9003868103 22.8427352905 PgISO 100pM 5 7.035276413 6.4739766121 6.7546265125 PgISO 100pM 5 17.7296905518 17.7815265656 17.7556085587 PgISO 100pM 5 7.035276413 6.4739766121 6.7546265125 PgISO 100pM 5 21.4203605652 21.7286834717 21.5745220184 PgISO 100pM 6 7.4083714485 7.1922330856 7.3003022671 PgISO 100pM 6 18.8875370026 17.5487861633 18.2181615829 PgISO 100pM 6 7.4083714485 7.1922330856 7.3003022671 PgISO 100pM 6 21.3548183441 21.5234069824 21.4391126633 PgISO 100pM 7 9.5879535675 9.6487112045 9.618332386 PgISO 100pM 7 19.8930778503 19.8432788849 19.8681783676 PgISO 100pM 7 9.5879535675 9.6487112045 9.618332386 PgISO 100pM 7 22.5527629852 22.1498851776 22.3513240814 PgISO 100pM 8 9.9407787323 9.7244720459 9.8326253891 PgISO 100pM 8 20.6256809235 20.5466861725 20.586183548 PgISO 100pM 8 9.9407787323 9.7244720459 9.8326253891 PgISO 100pM 8 23.3032951355 23.423784256 23.3635396957 image1.tiff image41.png