Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections Mariana Fávero Bonesso1,2, Silvio Alencar Marques3, Carlos Henrique Camargo1, Carlos Magno Castelo Branco Fortaleza2, Maria de Lourdes Ribeiro de Souza da Cunha1 1Departamento de Microbiologia e Imunologia, Instituto de Biociencias de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, SP, Brazil. 2Departamento de Doenças Tropicais, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, SP, Brazil. 3Departamento de Dermatologia e Radiologia, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, SP, Brazil. Submitted: September 17, 2013; Approved: April 17, 2014. Abstract The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medi- cal School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types detected in the seven mecA-positive S. aureus strains were type Ia in one, type II in three, and type IV in three. The PVL gene was detected in 10 (15.1%) in sensitive strains. Pulsed field gel electrophoresis revealed non-clonal diversity among the isolates. The risk factors associated with MRSA acquisition in this study were previous ciprofloxacin use and working in a healthcare environment. The risk factors in- dicate plausible routes of CA-MRSA transmission among the subjects studied. Key words: Staphylococcus aureus, resistance, skin infections, virulence, epidemiology. Introduction Staphylococcus aureus is a versatile pathogen associ- ated with infections in both hospital and community setting (Miranda et al., 2007, Schuenck et al., 2009, D’Agata et al., 2009). Due to its widespread use, treatment with penicillin became ineffective due to the high rate of resistance; conse- quently, semi-synthetic penicillin, such as methicillin, was developed as the main alternative against penicillin resis- tant strains. However soon before its introduction, Methicillin-Resistant S. aureus (MRSA) strains emerged in hospital environments (Jevons 1961) and then in the com- munity about 20 years later (Gosbell 2004). The main methicillin resistance mechanism is the production of PBP2a, a transpeptidase involved in bacterial wall synthesis, codified by the mecA gene (Chambers 1997) and carried in a mobile element called the staphylo- coccal cassette chromosome mec (SCCmec). SCCmec is classified according to the presence or absence of specific DNA regions (Turlej et al., 2011). So far, 11 main SCCmec types (I to XI, and subtypes) have been described (IWG- SCC). Usually, SCCmec types I, II and III are found in hos- pital environments (Hospital-Associated Methicillin Resis- tant S. aureus) while types IV, V and VI are associated with community settings (Community-Associated Methicillin Brazilian Journal of Microbiology 45, 4, 1401-1407 (2014) Copyright © 2014, Sociedade Brasileira de Microbiologia ISSN 1678-4405 www.sbmicrobiologia.org.br Send correspondence to: M.F. Bonesso. Departamento de Microbiologia e Imunologia, Instituto de Biociencias de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Caixa Postal 510, 18618-970 Botucatu, SP, Brazil. E-mail: mfbonesso@hotmail.com. Research Paper Resistant S. aureus). However, according to recent data, a change in the epidemiology of CA-MRSA has been re- ported (Cook and Brown 2010). Community-associated methicillin-resistant S. aureus (CA-MRSA)cause a broad range of skin and soft tissue infections such as furuncles, boils, carbuncles (Yao et al., 2010), cellulitis, and necrotizing fasciitis (Miller et al., 2005) in patients without traditional risk factors (Camp- bell et al., 2004). CA-MRSA strains usually carry the Pan- ton Valentine leukocidin (PVL) gene as the main virulence factor. PVL is a two-component pore-forming cytotoxin that acts on mitochondria and polymorphonuclear leuco- cytes wich indirectly leads to epithelial necrosis and in- vasiveness (Boyle-Vavra and Daum 2007). In addition to reports demonstrating the importance of PVL for MRSA infections (David et al., 2008; Goering et al., 2008; Ca- margo et al., 2013), methicillin-sensitive S. aureus (MSSA) strains have also been shown to carry the PVL gene and to be responsible for severe infections (D’Aze- vedo et al., 2009; Lee et al., 2010; Kreienbuehl et al., 2011). The objectives of the present study were to determine the prevalence of MRSA from a population attending an outpatient dermatology clinic, to evaluate associated risk factors, and to determine the virulence profile of S. aureus strains. Material and Methods Study design This was a prospective cross-sectional study involv- ing a convenience sample of outpatients presenting skin and/or soft tissue infections. The patients were seen at an emergency and/or outpatient dermatology clinic of the Bo- tucatu Medical School, between September 2008 and Sep- tember 2009. The patients provided written informed consent to participate in this study. The researcher was trained to interview the patients using a questionnaire to collect the following data: age, gender, residence area (ur- ban or rural), working in a healthcare environment, previ- ous institutional admission in the last 12 months (hospital, clinics, prison, sports team facilities), diabetes diagnosis, medical procedure in the last 12 months (surgery, dialysis, catheterization, drainage), illicit drug intake, sexual orien- tation, length of infection, antibiotic usage, sports practice, work status and income. We defined working in healthcare environment (healthcare worker) as a professional who di- rectly assists patients. Inclusion criteria The patients were screened by the dermatologist dur- ing a medical consultation. Patients presenting active infec- tions at the time of consultation who met the inclusion criterion for infections were included in the study. Infec- tions included in this criterion were diagnoses for: boils, cellulitis, erysipelas, abscesses, hidradenitis, folliculitis, diabetes-related infected foot, suppurative osteomyelitis, secondarily infected dermatitis, secondarily infected trau- matic lesions, and bullous impetigo. We considered pa- tients who were hospitalized and healthcare workers to be at risk. Collection and bacterial isolation A moistened (sterile saline) swab was used to collect the clinical material. The cultures were immediately sent to the laboratory and streaked onto Sheep Blood Agar and Mannitol Salt agar and incubated at 37 °C. Staphylococcus spp. colonies were identified based on Gram stain, catalase and coagulase tube production. The isolates were stored in nutrient broth with glycerol at -70 °C. Detection of methicillin resistance and antimicrobial susceptibility testing The disk diffusion test was performed using oxacillin (1 �g) and cefoxitin (30 �g) disks as described by the Clini- cal Laboratory Standards Institute (CLSI 2009). Addi- tionally, we used the mecA gene detection as the gold standard for evaluating MRSA. The plates were incubated at 35 °C. Susceptibility to penicillin (10 U), erythromycin (15 �g), clindamycin (2 �g), sulfamethoxazole/trimetho- prim (125 �g/23.75 �g), gentamicin (10 �g)and linezolid (30 �g) was tested according to CLSI guidelines. The mecA gene was detected by polymerase chain reaction (PCR) (Murakami et al., 1991) after bacterial DNA extraction (Pereira et al., 2009), and a positive result defined MRSA. Reference strains were included in all reactions as positive (S. aureus ATCC 33591) and negative (S. aureus ATCC 25923) controls for PCR reactions. Characterization of S. aureus SCCmec SCCmec typing was performed for mecA positive isolates only, using the primers and multiplex PCR parame- ters described by Milheiriço et al. (2007). The following reference strains were used: COL for SCCmec type I; N315 for SCCmec type Ia; PER34 for SCCmec type II; AN546 for SCCmec type III; HU25 for SCCmec type IIIa, and MW2 for SCCmec type IV. PCR detection of lukF-PV and lukS-PV and TSST-1 genes The genes and parameters standardized by Lina et al. (1999) were used for detecting the PVL genes (lukF-PV and lukS-PV). The toxic shock syndrome toxin 1 (TSST-1)gene was detected as proposed by Johnson (1991) and optimized by Cunha et al. (2006). S. aureus ATCC 49775 and S. aureus ATCC 29213 were used as positive and negative controls, respectively, in all reactions. 1402 Bonesso et al. PFGE of MRSA isolates MRSA typing was performed by macrorestriction of genomic DNA of the strains, followed by PFGE according to McDougal et al. (2003). The gels were stained with the GelRed system (10,000x in water; Biotium, USA) for 1 h and photographed under UV transillumination. The Bio- Numerics software, version 6.1 (AppliedMaths, Belgium) was used for similarity analysis, calculation of the Dice cor- relation coefficient, and construction of a dendrogram by UPGMA (unweighted pair group method using arithmetic averages). Band position tolerance and optimization were set at 1.25% and 0.5%, respectively. A similarity coeffi- cient of 80% was chosen for the determination of clusters (McDougal et al., 2003). CA-MRSA representative clones (USA 500, USA400, USA 100, USA 800, USA 300, HU-25, EMRSA 15, JCSC 4469, OSPC, MR 108, NCTC 10422) were used for comparison in PFGE analysis. Statistical analysis of risk factors Risk factors were analyzed according to the demo- graphic data collected (described in study design) from the population studied. The data were analyzed using the Epi Info® 3.5.2 (Centers for Disease Control and Prevention, Atlanta, USA) and SPSS 15.0 programs. The results were submitted to univariate analysis. Di- chotomous variables were analyzed by the chi-squared or Fisher’s exact test. The Mann-Whitney test was applied to the analysis of numerical variables and to multivariate anal- ysis using a logistic regression model. p values < 0.05 and > 0.1 were used for inclusion and exclusion of the variables in the model, respectively. Results A total of 127 swabs,one per patient,were obtained from patients seen in the emergency department and/or der- matology outpatient clinic during the study period, from which S. aureus was isolated in 66 (52%). Coagulase- negative Staphylococcus strains were isolated in 42 (33%) patients and the remaining 19 (15%) patients presented negative culture for Staphylococcus. S. aureus were iso- lated from secondarily infected dermatitis/secondarily in- fected traumatic lesions (26 strains, 39.4%), ingrown toe- nail infection (13 strains, 19.7%), boils (8 strains, 12.1%), bullous impetigo (6 strains, 9.1%), diabetes-related in- fected feet (4 strains, 6.1%) cellulitis, osteomyelitis (3 strains, 4.5%, each), folliculitis, erysipelas and necrotic tis- sue (1 strain, 1.5%, each). Identification of MRSA by the disk diffusion method and mecA gene detection Nine (13.6%) S. aureus strains were resistant to oxa- cillin and eight (12.1%) were resistant to cefoxitin. The mecA gene was detected in seven (10.6%) isolates. Of these, six (85.7%) were resistant to oxacillin and six (85.7%) to cefoxitin. On the other hand, among the mecA negative strains, 3 presented oxacillin resistance and 2 pre- sented cefoxitin resistance. SCCmec typing of S. aureus and susceptibility profile of MRSA Among the seven S. aureus strains carrying the mecA gene, one (14.2%) was classified as type Ia, three (42.9%) as type II, and three (42.9%) as type IV. S. aureus carrying SCCmec type Ia was only resistant to penicillin. The three strains carrying type II were resistant to cefoxitin, clin- damycin, erythromycin, oxacillin, and penicillin. The three SCCmec type-IV isolates were resistant to cefoxitin, oxa- cillin and penicillin, but only one strain each (33.3%) was resistant to clindamycin and erythromycin. Detection of the lukS-PV/lukF-PV genes and TSST-1 gene in S. aureus The TSST-1 gene was not detected in any of the strains studied. The lukSF-PV gene was detected in 10 (15.1%) strains, only in methicillin-sensitive strains. Among the 10 S. aureus strains carrying the PVL genes, six (60%) were recovered from furuncles, two (20%) from sec- ondary infections, and two (20%) from impetigo. PFGE of MRSA Four isolates containing SCCmec II presented the same pulsotype in PFGE, including a bloodstream infection strain (070H) recovered from a patient with suppurative osteomyelites. Strains with SCCmec IV presented other band profiles and were grouped in another cluster with sim- ilarity of 80.8%. The only SCCmec Ia strain was not be al- located to any cluster. None of the evaluated strains were grouped with circulating known clones (Figure 1). Analysis of the results One risk factor for MRSA acquisition identified in the present study by univariate analysis was previous use of ciprofloxacin [OR: 8.75 (1.59-48.29), p = 0.04]. In addition to ciprofloxacin use, multivariate analysis revealed that working in a healthcare environment [OR:17.5 (1.22-250.36), p = 0.04]was also associated with the acqui- sition of MRSA. However, the latter finding is questionable since only one patient infected with MRSA worked in a healthcare setting (Table 1). Discussion In a non-outbreak context of outpatients with skin and soft tissue infection, S. aureus was the most frequently iso- lated Staphylococcus species. The presence of S. aureus as the main agent of skin and soft tissue infections highlights the importance of identifying this pathogen. The high fre- quency of isolation of S. aureus is a common phenomenon in patients with infections such as cellulitis, impetigo, fu- CA-MRSA non outbreak infection 1403 runcles, carbuncles, and folliculitis (Stullberg et al., 2002), reaching a frequency of up to 72% (Issartel et al., 2002). The PVL gene was detected in 15.1% of the S. aureus strains studied here. The presence of leukocidin in commu- nity-acquired strains has been extensively documented and is associated with methicillin resistance (Francis et al., 2005; Miller et al., 2005). However, in the present study PVL was not detected in methicillin-resistant strains, only in methicillin-sensitive strains. Demir et al. (2012) detected the PVL gene in 9% of S. aureus strains isolated from skin and soft tissue infections, but in other studies, the gene was absent in MRSA strains (Takizawa et al., 2005; Lee et al., 2010), supporting our findings. None of the S. aureus or MRSA isolates carried the TSST-1 gene. The absence of PVL and TSST-1 gene in CA-MRSA strains isolated from skin and soft tissue infec- tions was also verified in another study (Lee et al., 2010) suggesting involvement of other pathogenicity factors in such isolates. The mecA gene was detected in seven strains isolated from skin infections of outpatients. SCCmec typing re- vealed the presence of types Ia, II and IV. A higher preva- lence of types II and IV was also reported by Kikuta et al. (2011) in Japanese children with impetigo. In Brazil, detec- tion of SCCmec type II was previously shown to be associ- ated with hospital settings (Miranda et al., 2007; Schuenck et al., 2009). However, our findings indicate that patients who presented MRSA with this SCCmec type did not pres- ent an association with hospital environments nor with the healthcare worker included in our analysis. The presence of such diversity types of SCCmec could be explained by the patient origin. They came from a community previously seen in an ambulatory care center, suggesting that this may constitute an intermediate zone. This zone may have the most variable types of SCCmec, characterizing by the prevalence and likely a coexistence of both CA- and HA-MRSA (Kouyos et al., 2013). In a Brazilian study published in 2005, Ribeiro et al., described the first cases of CA-MRSA infections in Brazil. The MRSA isolates were collected from two patients pre- senting skin and soft tissue infections, and from another pa- tient presenting septic arthritis. The three patients were from the community and had no risk factors for MRSA ac- quisition, such as the presence of medical devices, hospital- ization or surgery in the last 12 months before MRSA isolation. Due to these characteristics, the origin of infec- tion was considered community acquired. The samples were obtained between June 2002 and September 2003 in the outpatient clinic from two different hospitals in Porto Alegre, Rio Grande do Sul, Brasil. All samples harbored the SCCmec IV and presented identical profiles compared to the Ocean Southeast Pacific Clone (OSPC). In another study, Ribeiro and cols (2007) characterized CA-MRSA isolated in Rio de Janeiro and Porto Alegre. Besides the 1404 Bonesso et al. Figure 1 - PFGE analysis of MRSA isolates from skin and soft tissue infections. Three SCCmec II samples (070, 064 and 126) had identical profiles, while three SCCmec IV samples (064, 022 and 038) grouped with high similarity, and one sample (016) could not be allocated in any group. OSPC clone, they have found the USA300 and USA400 (Ribeiro et al., 2007). Other Brazilian studies verified that most samples carrying SCCmec IV presented identical pro- files as the pediatric clone/USA800 (Miranda et al., 2007; Scribel et al., 2009; Carmo et al., 2011) and OSPC (Scribel et al., 2009) and strains highly associated with the USA400 clone (Schuenck et al., 2009). In Sao Paulo, Brazil, a new ST1176 strain emerged from a mutation in the yqiL locus, probably originating from the pediatric clone (Carmo et al., 2011). In our study the strains harboring the SCCmec IV were highly associated with each other, but lacked associa- tion with the most common clones by PFGE. In a study conducted in Europe, a large number of strains could not be related to previously known clones, a finding that might be explained by the wide diversity of CA-MRSA strains (Rolo et al., 2012). The SCCmec II strains presented identical PFGE pro- files. The strain SCCmec II isolated from one patient (pa- tient number 070), that was isolated from osteomyelitis and bloodstream infection as well. It seems to be an invasive strain that deserves further characterization. The most con- CA-MRSA non outbreak infection 1405 Table 1 - Statistical analysis ofdemographic data. Univariate and Multivariate analysis reveal the previous use of ciprofloxacin as a risk factor for CA-MRSA acquisition, and multivariate analysis showed that healthcare work was a risk factor. The risk factor in univariate analysis is highlighted in red and the values (number of cases, non-cases, OR and p values) are in bold. We highlighted in yellow the additional risk factors according to multivatiate analysis. Risk factor Univariate analysis Multivariate analysis Cases (n = 7) Non-cases (n = 119) OR (95%CI) p OR (95%CI) p Demographic data Female gender 6 (89.7) 57 (47.9) 6.53 (0.76-55.88) 0.052 Age in years mean 48 (13-72) 46 (0-82) ... 0.64 Urban residence 7 (100.0) 107 (89.9) ... 1.00 Working in a rural area 1 (14.3) 16 (13.4) 1.07 (0.12-9.51) 1.00 Working in a healthcare environment 1 (14.3) 2 (1.7) 9.75 (0.77-123.23) 0.16 17.5 (1.22-250.36) 0.04 Retired 2 (28.6) 18 (15.1) 2.24 (0.40-12.57) 0.31 Wage > R$ 1 000.00 per month* 2 (28.6) 35 (29.4) 0.96 (0.18-5.19) 1.00 Previous hospital admission and procedures Previous hospital admission 2 (28.6) 20 (16.8) 1.98 (0.36-10.93) 0.61 Previous surgery 1 (14.3) 10 (8.4) 1.82 (0.20-16.62) 0.48 Abscess drainage 0 (0.0) 6 (5.0) 0.0 (...) 1.00 Underlying comorbidity Diabetes mellitus 2 (28.6) 21 (17.6) 1.87 (0.34-10.28) 0.61 Use of insulin 1 (14.3) 6 (5.0) 3.14 (0.32-30.40) 0.34 Underlying dermatological disease 0 (0.0) 4 (3.4) 0.0 (...) 1.00 Characteristics of present infection Secondary to local trauma 3 (42.9) 49 (41.2) 1.07 (0.23-5.00) 1.00 Duration > 30 days 4 (57.1) 56 (47.1) 1.50 (0.32-6.99) 0.78 History of antibiotic use Any antibiotic 4 (57.1) 40 (33.6) 2.63 (0.56-12.34) 0.24 Penicillin benzathine 0 2 (1.7) 0.0 (...) 1.00 Amoxicillin 2 (18.6) 16 (13.4) 2.58 (0.46-14.14) 0.26 Cephalexin 0 (0.0) 26 (21.4) 0.0 (...) 0.34 Clindamycin 2 (28.6) 11 (9.2) 3.93 (0.68-22.67) 0.15 Ciprofloxacin 3 (42.9) 12 (10.1) 6.69 (1.34-33.41) 0.04 8.75 (1.59-48.29) 0.01 Macrolides 0 (0.0) 3 (2.5) 0.0 (...) 1.00 Other Participation in sports 0 (0.0) 17 (14.3) 0.0 (...) 0.59 Use of illicit drugs 0 (0.0) 12 (10.1) 0.0 (...) 1.00 Results are numbers (percentage), unless otherwise stated. *Approximately US$ 500.00 per month. OR, odds ratio; CI, confidence interval. cerning fact is its high similarity and presence in patients not related to each other. Risk factor analysis revealed that patients carrying MRSA had a history of ciprofloxacin use and worked in a healthcare environment. The use of fluoroquinolones, par- ticularly ciprofloxacin, has been shown to contribute to col- onization with MRSA. S. aureus is highly resistant to fluoroquinolones while in sub-inhibitory concentrations can increase the adhesin expression (Bisognano et al., 2004, 1997) thus leading to the success on MRSA coloniza- tion. Healthcare workers play an important role in the dis- semination of CA-MRSA in hospital settings and have been responsible for hospital outbreaks(Maltezou et al., 2009, Tang et al., 2007). Healthcare workers are prone to contracting community-associated resistant strains and rep- resent an important source of infection with CA-MRSA for patients (Alia et al., 2012; Maltezou et al., 2009; Wagenlehner et al., 2008; Tang et al.; 2007; Raab et al., 2006). In our study, we verified that healthcare working was a risk factor for acquiring MRSA. However, this is a matter that needs to be validated by more specific studies and careful analysis in this regard. In conclusion, we verified a low prevalence of MRSA in outpatients with skin infections, with strains not related with known MRSA clones, indicating a possible geo- graphic clonal pattern. Risk factors indicate plausible routes of transmission since the use of ciprofloxacin and working in a healthcare environment are associated with the persistence of strains and dynamics of pathogen trans- mission. These findings have significant importance in CA-MRSA epidemiology. 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