Bergamo, Debora Cristina B. [UNESP]Kato, Massuo JorgeBolzani, Vanderlan da Silva [UNESP]Furlan, Maysa [UNESP]2014-05-272014-05-272005-11-01Journal of the Brazilian Chemical Society, v. 16, n. 6 B, p. 1406-1409, 2005.0103-5053http://hdl.handle.net/11449/68470The biosynthetic origins of the isoprene units of 4-nerolidylcatechol (1), the major constituent of Potomorphe umbellata, have been studied through feeding experiments with [14C]- and [13C]-glucose, and with precursors of the mevalonic acid and triose/pyruvate pathways, namely, [2- 14C]-mevalonolactone and [U-14C]-glyceraldehyde-3- phosphate, respectively. The pattern of incorporation of label from [1- 13C]-glucose into 1 was determined by quantitative 13C NMR spectroscopy. The labelling pattern revealed that the additive was specifically incorporated, and that the isoprene units of the sesquiterpenoid moiety of 4-nerolidylcatechol were derived from both the mevalonic acid and the triose/pyruvate pathways. The results indicate that both plastidic and cytoplasmic pathways are able to provide isopentenyl diphosphate units for the biosynthesis of 1. ©2005 Sociedade Brasileira de Química.1406-1409eng4-nerolidylcatecholBiosynthesis, sesquiterpenoidIsopentenyl diphosphatePiperaceaePotomorphe umbellataArtigo10.1590/S0103-50532005000800018S0103-50532005000800018WOS:000234078700018Acesso aberto2-s2.0-308444740042-s2.0-30844474004.pdf44840836852516731308042794786872