Garavello, I. [UNESP]Baranauskas, V.Da Cruz-Höfling, Maria Alice2014-05-272014-05-272004-01-01Histology and Histopathology, v. 19, n. 1, p. 43-48, 2004.0213-3911http://hdl.handle.net/11449/67615The influence of He-Ne laser radiation on the formation of new blood vessels in the bone marrow compartment of a regenerating area of the mid-cortical diaphysis of the tibiae of young adult rats was studied. A small hole was surgically made with a dentistry burr in the tibia and the injured area received a daily laser therapy over 7 or 14 days transcutaneously starting 24 h from surgery. Incident energy density dosages of 31.5 and 94.5 Jcm-2 were applied during the period of the tibia wound healing investigated. Light microscopic examination of histological sections of the injured area and quantification of the newly-formed blood vessels were undertaken. Low-level energy treatment accelerated the deposition of bone matrix and histological characteristics compatible with an active recovery of the injured tissue. He-Ne laser therapy significantly increased the number of blood vessels after 7 days irradiation at an energy density of 94.5 Jcm-2, but significantly decreased the number of vessels in the 14-day irradiated tibiae, independent of the dosage. These effects were attributed to laser treatment, since no significant increase in blood vessel number was detected between 8 and 15 non-irradiated control tibiae. Molecular mechanisms involved in low-level laser therapy of angiogenesis in post-traumatic bone regeneration needs further investigation.43-48engAngiogenesisBone wound healingHe-Ne laserRatTibiaangiogenesisanimal experimentanimal modelanimal tissuebone injurybone marrowbone matrixbone regenerationcomparative studycontrolled studycortical bonedensitydiaphysishelium neon laserhistologylow level laser therapymalemicroscopymolecular dynamicsnonhumanquantitative analysisradiation doserattibiawound healingAnimalsHeliumLaser Therapy, Low-LevelLasersMaleNeonNeovascularization, PhysiologicRatsRats, WistarTime FactorsWound HealingAnimaliaArtigoAcesso aberto2-s2.0-16424744142-s2.0-1642474414.pdf