Sanita, Paula Volpato [UNESP]Zago, Chaiene Evelin [UNESP]Oliveira Mima, Ewerton Garcia de [UNESP]Pavarina, Ana Claudia [UNESP]Jorge, Janaina Habib [UNESP]Machado, Ana Lucia [UNESP]Vergani, Carlos Eduardo [UNESP]2014-12-032014-12-032014-07-01Oral Surgery Oral Medicine Oral Pathology Oral Radiology. New York: Elsevier Science Inc, v. 118, n. 1, p. 84-91, 2014.2212-4403http://hdl.handle.net/11449/112544Objective. To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates).Study Design. After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (alpha = .05).Results. C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata.Conclusions. C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.84-91engArtigo10.1016/j.oooo.2014.03.020WOS:000338192600017Acesso restrito8867670539105403800024878184258730031305224278200000-0002-7375-4714