Castelli, E.C. [UNESP]Gil, D.S. [UNESP]Veiga, L.C.S.de Camargo, J.L.V. [UNESP]2014-05-202014-05-202005-06-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 6, p. 837-842, 2005.0100-879Xhttp://hdl.handle.net/11449/12834In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.837-842engHuman leukocyte antigen-AHLA-ATypingRFLPPolymorphismMajor histocompatibility complexArtigo10.1590/S0100-879X2005000600004S0100-879X2005000600004Acesso abertoS0100-879X2005000600004.pdf