Magalhaes, Lizandra G.de Souza, Julia M.Wakabayashi, Kamila A. L.Laurentiz, Rosangela da S. [UNESP]Vinholis, Adriana H. C.Rezende, Karen C. S.Simaro, Guilherme V.Bastos, Jairo K.Rodrigues, VanderleiEsperandim, Viviane R.Ferreira, Daniele S.Crotti, Antonio E. M.Cunha, Wilson R.Silva, Marcio L. A. E.2014-05-202014-05-202012-05-01Parasitology Research. New York: Springer, v. 110, n. 5, p. 1747-1754, 2012.0932-0113http://hdl.handle.net/11449/10109In this paper, cercariae, schistosomula, and adult Schistosoma mansoni worms were incubated in vitro with the essential oil of Piper cubeba (PC-EO) at concentrations from 12.5 to 200 mu g/mL, and the viability was evaluated using an inverted microscopy. The effects of PC-EO at 100 and 200 mu g/mL on the stages of S. mansoni were similar to those of the positive control (PZQ at 12.5 mu g/mL), with total absence of mobility after 120 h. However, at concentrations from 12.5 to 50 mu g/mL, PC-EO caused a reduction in the viability of cercariae and schistosomula when compared with the negative control groups (RPMI 1640 or dechlorinated water) or (RPMI 1640 + 0.1% DMSO or dechlorinated water + 0.1% DMSO). on the other hand, adult S. mansoni worms remained normally active when incubated with PC-EO at concentrations of 12.5 and 25 mu g/mL, and their viabilities were similar to those of the negative control groups. In addition, at concentrations ranging from 50 to 200 mu g/mL, separation of all the coupled adult worms was observed after 24 h of incubation, which is related to the fact of the reduction in egg production at this concentration. The main chemical constituents of PC-EO were identified by gas chromatography-mass spectrometry as being sabinene (19.99%), eucalyptol (11.87%), 4-terpineol (6.36%), beta-pinene (5.81%), camphor (5.61%), and delta-3-carene (5.34%). The cytotoxicity of the PC-EO was determined, and a significant cytotoxicity was only obtained in the concentration of 200 mu g/mL after 24 h treatment. The results suggest that PC-EO possesses an effect against cercariae, schistosomula, and adult worms of the S. mansoni.1747-1754engArtigo10.1007/s00436-011-2695-7WOS:000302814700020Acesso restrito