Buzinaro, M. G.Freitas, PPSKisiellius, J. J.Ueda, M.Jerez, J. A.2014-05-202014-05-202003-09-01Veterinary Journal. London: Bailliere Tindall, v. 166, n. 2, p. 185-187, 2003.1090-0233http://hdl.handle.net/11449/2423To determine the incidence of rotavirus infection among dairy herds in the State of Sdo Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil. (C) 2003 Elsevier B.V. Ltd. All rights reserved.185-187engpicobirnavirusdiarrhoeaCattlePAGEArtigo10.1016/S1090-0233(03)00031-5WOS:000184882400011Acesso restrito