Sonon, PaulinSadissou, IbrahimTokplonou, LéonidasM'po, Kuumaaté K.G.Glitho, Sonya S.C.Agniwo, PrivatIbikounlé, MoudachirouMassaro, Juliana DoblasMassougbodji, AchilleMoreau, PhilippeSabbagh, AudreyMendes-Junior, Celso T.Moutairou, Kabirou A.Castelli, Erick C. [UNESP]Courtin, DavidDonadi, Eduardo A.2019-10-062019-10-062018-12-01Molecular Immunology, v. 104, p. 108-127.1872-91420161-5890http://hdl.handle.net/11449/189672HLA-G/E/F genes exhibit immunomodulatory properties and are expressed in placenta. Little attention has been devoted to the study of these genes in sub-Saharan African populations, which are yet the most diverse. To fill this gap, we evaluated the complete gene variability, approximately 5.1 kb for HLA-G (n = 149), 7.7 kb for HLA-E (n = 150) and 6.2 kb for HLA-F (n = 152) in the remote Beninese Toffin population, using massive parallel sequencing. Overall, 96, 37 and 68 variable sites were detected along the entire HLA-G, -E and -F, respectively, arranged into region-specific haplotypes; i.e., promoter haplotypes (16, 19, and 15 respectively), coding haplotypes (19, 15, and 29 respectively), 3’ untranslated region (3′UTR) haplotypes (12, 7 and 2, respectively) and extended haplotypes (33, 31 and 32 respectively). All promoter/coding/3'UTR haplotypes followed the patterns already described in worldwide populations. HLA-E was the most conserved, exhibiting mainly two full-length encoded-molecules (E*01:01 and E*01:03), followed by HLA-F, three full-length proteins (F*01:01, F*01:02 and F*01:03) and HLA-G, four proteins: three full-length (G*01:01, G*01:03 and G*01:04) and one truncated (G*01:05N). Although HLA-G/E/F alleles in the Toffin population were the most frequently observed worldwide, the frequencies of the coding haplotypes were closely similar to those described for other African populations (Guinea-Conakry and Burkina-Faso), when compared to non-African ones (Brazilian), indicating that variable sites along these genes were present in Africa before human dispersion.108-127engAfricanHaplotypesHLA-EHLA-FHLA-GMassive parallel sequencingArtigo10.1016/j.molimm.2018.08.016Acesso aberto2-s2.0-85056528348