Silva Rascado, T. da [UNESP]Rigatto Martins, L. [UNESP]Watanabe Minto, B. [UNESP]Sa Lorena, S. E. R. de [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]2013-09-302014-05-202013-09-302014-05-202010-09-01Theriogenology. New York: Elsevier B.V., v. 74, n. 4, p. 596-601, 2010.0093-691Xhttp://hdl.handle.net/11449/14590The objective was to evaluate the parthenogenetic activation of domestic cat oocytes. Cumulus-oocyte complexes matured for 36 h were subjected to three protocols of parthenogenetic activation: Group 1 - ionomycin + cycloheximide; Group 2 - ionomycin + roscovitine; and Group 3 - ionomycin + strontium. As a control, a fourth group of oocytes were cultured in the absence of any activation agent. In all groups, embryos were cultured in SOFaa for 72 h after activation and evaluated for activation rate, cleavage, and embryonic development using Hoechst 33342. There were no significant differences among the three treated groups for rates of activated oocytes (70.1 +/- 4.3, 75.5 +/- 4.7, and 61.9 +/- 7.2%, for Treatments 1, 2, and 3 respectively; mean +/- SEM), or cleavage (48.1 +/- 5.9, 47.4 +/- 3.8, and 33.3 +/- 6.8%). However, activation and cleavage rates were higher (P < 0.05) than those in the control group (35.5 +/- 6.4 and 11.8 +/- 4.0%). There were no significant differences among treatment groups for proportion of embryos with 2-10 cells, 10-16 cells, and morulas. In the Control group, the embryo production rate was lower (P < 0.05), although the activation rate was high. The authors concluded that all three treatments effectively induced parthenogenetic activation of domestic cat oocytes. However, to optimize the use of strontium and roscovitine, a dose response and the effect of the presence of Ca(++) in the medium requires further study. (C) 2010 Elsevier B.V. All rights reserved.596-601engStrontiumRoscovitineCycloheximideArtificial activationFeline oocytesArtigo10.1016/j.theriogenology.2010.03.010WOS:000281261600014Acesso restrito