Peres, O.Oliveira, C. H.Barrientos-Astigarraga, R. E.Rezende, V. M.Mendes, G. D.Nucci, G. de2015-03-182015-03-182004-01-01Arzneimittel-forschung-drug Research. Aulendorf: Ecv-editio Cantor Verlag Medizin Naturwissenschaften, v. 54, n. 6, p. 314-319, 2004.0004-4172http://hdl.handle.net/11449/116876An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection was developed for the determination of pantoprazole (CAS 102625-70-7) in human plasma using lansoprazole (CAS 103577-45-3) as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid/liquid extraction using diethyl-ether/dichloromethane (70:30; v/v) and chromatographed on a C-8 analytical column. The mobile phase consisted of acetonitrile/water/methanol (57:25:18; v/v/v) + 10 mmol/l acetic acid + 20 mmol/l ammonium acetate. The method has a chromatographic total run time of 4.5 min and was linear within the range 5.0-5,000 ng/mL. Detection was performed on a triple quadrupole tandem mass spectrometer by Multiple Reaction Monitoring (MRM). The intra- and inter-run precisions calculated from quality control (QC) samples were 4.2% and 3.2%, respectively. The accuracies as determined from QC samples were -5.0% (intra-run) and 2.0% (inter-run). The method herein described was employed in a bioequivalence study of two tablet formulations of pantoprazole.314-319engCAS 102625-70-7pantoprazole, determination in human plasmaproton pump inhibitorArtigo10.1055/s-0031-1296977WOS:000222617400002Acesso restrito