Schwardt, Tatianna Frate [UNESP]Vides, Juliana Peloi [UNESP]Pacheco, Acácio Duarte [UNESP]Langoni, Hélio [UNESP]Generoso, Diego [UNESP]Machado, Gisele Fabrino [UNESP]Laurenti, Márcia DalastraMarcondes, Mary [UNESP]2014-05-272014-05-272012-12-01Brazilian Journal of Veterinary Research and Animal Science, v. 49, n. 6, p. 442-451, 2012.1413-95961678-4456http://hdl.handle.net/11449/74028Visceral leishmaniasis in dogs is described as a chronic disease whose main symptoms are progressive weigth loss, cachexy and dermatologic lesions. Recently, the disease has been associated to neurologic disorders. A total of 40 dogs with visceral leishmaniasis were divided into two groups. The first composed of dogs without neurological signs (n=30) and the second by dogs with neurological disorders (n=10). Brain samples were collected, stored in 10% buffered formalin and subjected to immunohistochemical examination for amastigotes forms of Leishmania (Leishmania) infantum chagasi, CD3+, CD4+ and CD8+ T lymphocytes and macrophages. Imunnohistochemistry evaluation revealed no amastigote forms of the parasite. CD3+ T lymphocytes were present in 24/30 (80%) dogs without neurological signs and in all dogs from the second group (p=0.0011). CD4+ and CD8+ were rarely observed, with CD4+ immunostaining in 10/40 (25%) dogs, from which half of them had neurological disease (p=0.0090). The presence of CD8+ was detected only in 4/10 (40%) dogs from neurological group (p=0.0021). Macrophages were detected in 38/40 (95%) dogs, without significant differences between groups (p=0.7664).442-451engLeishmania (Leishmania) infantum chagasiMacrophagesTCD3+TCD4+TCD8+ArtigoAcesso aberto2-s2.0-848764983702-s2.0-84876498370.pdf031040555812563418179466710900101817946671090010