Baron, Alessandra MachadoRodrigues, Ricardo de SousaSante, Luis Guilherme GianninaKister, Jocácia Muriele de MirandaNascimento, Valéria Marta Gomes do [UNESP]Bail, Alesandro2023-03-012023-03-012022-01-01Biocatalysis and Biotransformation.1029-24461024-2422http://hdl.handle.net/11449/239978Metal-organic frameworks (MOFs) are versatile materials because they have a large internal surface area and tuneable pores, making them suitable for enzyme immobilization. In this study, we prepared a typical microporous Fe-BDC MOF through a thermal treatment to produce additional meso and macropores interconnected to each other, capable of immobilizing the Burkholderia lata LBBIO-BL02 (BLL) lipase by entrapment and physical adsorption. The immobilization efficiency (E) was 90%, and the activity retention (R) was 400% (pNPP hydrolysis). The immobilized lipase (BLL@BDC) also showed excellent activity in the hydrolysis of vegetable oils in aqueous medium, achieving up to 3,200 U g−1 for olive oil, as well as high stability in organic solvents, especially for polar ones, such as iso-propanol (101.5 ± 2.6%), ethanol (103.0 ± 6.0%) and acetone (107.7 ± 8.3%). The results indicate that the multiporous Fe-BDC MOF is a promising support for lipase immobilization and further application in biocatalysis performed in organic media.engbiocatalysishydrolysisimmobilizationLipasesmetal–organic frameworksArtigo10.1080/10242422.2022.20683712-s2.0-85129285590