David, S.Sutre, A. F.Sanca, A.Mane, A.Henriques, V.Portugal, C.Sancho, L.Cardoso, A.Paixao, E.Duarte, E. L.Leite, Clarice Queico Fujimura [UNESP]Salem, J. I.Antunes, A.2014-05-202014-05-202012-11-01International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.1027-3719http://hdl.handle.net/11449/42034The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.1535-1537engMycobacterium tuberculosisrpoBsputum smear microscopysodium hypochloritedenaturing high-performance liquid chromatography (DHPLC) sequencingArtigo10.5588/ijtld.11.0658WOS:000310173900020Acesso aberto2114570774349859