Lopez, Silvia Noeli [UNESP]Lopes, Adriana Aparecida [UNESP]Batista, Joao Marcos [UNESP]Flausino, Otavio [UNESP]Bolzani, Vanderlan da Silva [UNESP]Kato, Massuo JorgeFurlan, Maysa [UNESP]2014-05-202014-05-202010-06-01Bioresource Technology. Oxford: Elsevier B.V., v. 101, n. 12, p. 4251-4260, 2010.0960-8524http://hdl.handle.net/11449/26077The ability to carry out geranylations on aromatic substrates using enzymatic extracts from the leaves of Piper crassinervium (Piperaceae) was evaluated. A literature analysis pointed out its importance as a source of prenylated bioactive molecules. The screening performed on aromatic acceptors (benzoic acids, phenols and phenylpropanoids) including geranyl diphosphate as prenyl donor, showed the biotransformation of the 3,4-dihydroxybenzoic acid by the crude extract, and the p-hydroxybenzoic acid by both the microsomal fraction and the crude extract, after treating leaves with glucose. The analysis of the products allowed the identification of C- and O-geranylated derivatives, and the protease (subtilisin and pepsin) inhibition performed on the O-geranylated compounds showed weak inhibition. Electrophoretic profiles indicated the presence of bands/spots among 56-58 kDa and pI 6-7, which are compatible with prenyltransferases. These findings show that P. crassinervium could be considered as a source of extracts with geranyltransferase activity to perform biotransformations on aromatic substrates. (C) 2010 Elsevier Ltd. All rights reserved.4251-4260engPiper crassinerviumPiperaceaeBiotransformationGeranylationArtigo10.1016/j.biortech.2010.01.041WOS:000276374500001Acesso restrito44840836852516731308042794786872