Gremiao, MPDCelli, C. M.Chaimovich, H.2014-05-202014-05-201996-04-01Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 29, n. 4, p. 489-494, 1996.0100-879Xhttp://hdl.handle.net/11449/31581Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 mu g/ml) from the same sera, not containing beta(2)GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.489-494enganticardiolipin antibodiescardiolipinvesiclessyphilissystemic lupus erythematosuscarboxyfluoresceinArtigoWOS:A1996UE87000011Acesso restrito