Silva, Roberta Fernanda da [UNESP]Sertório, Jonas Tadeu CauLacchini, RiccardoTrapé, Atila AlexandreTanus-santos, José EduardoRush, James W. E.Amaral, Sandra Lia do [UNESP]Zago, Anderson Saranz [UNESP]2015-08-062015-08-062014Aging Clinical and Experimental Research, v. 26, n. 6, p. 591-598, 2014.1720-8319http://hdl.handle.net/11449/126068The purpose of this study was to evaluate the relationship between 3 eNOS gene polymorphisms and training status (TS) in affecting plasma nitrite concentration (NO2) in normotensive adults over 50 years old. Resting blood pressure (BP) was measured in all participants (n = 101). Plasma was taken to analyze: lipid profile, nitrite concentration (NO2) and lipid peroxide levels (T-BARS). Also, genomic DNA was extracted from plasma for genotyping NOS3 polymorphisms (-786T[C; 894G[T; and VNTR in intron 4). TS was determined by one-mile walk test and Functional Fitness Test Battery from AAHPERD (TS1—regular TS; TS2—good TS; and TS3—very good TS). BP was not influenced by TS, but NO2 was 15 % higher in TS3 (123 ± 27 nM) compared to TS-2 (106 ± 22 nM). No differences were found in plasma NO2 in the haplotype analyses. However, the presence of the C allele (T-786C) and ASP allele (Glu298Asp) was found to enhance the correlation between TS and NO2 levels (r = 0.492 in C/4b/ASP haplotype and r = 0.855 in C/4a/ASP haplotype). This study thus identifies NOS3 polymorphism-dependent sensitivity to the effects of physical training on plasma NO2. Maintenance of good levels of training status, in carriers of C allele for T-786C polymorphism, combined with ASP allele for Glu298Asp polymorphism, may result in an increase in the NO2 plasma concentrations, which may reflect improved NO bioavailability in older adult normotensive individuals.591-598engTraining statusNitric oxideeNOS haplotypeAdultsElderlyNormotensive IntArtigo10.1007/s40520-014-0218-yAcesso restrito2030708742766455